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- PDB-6g01: Complex of neuraminidase from H1N1 influenza virus with tamiphosp... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6g01 | |||||||||
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Title | Complex of neuraminidase from H1N1 influenza virus with tamiphosphor monomethyl ester | |||||||||
![]() | Neuraminidase | |||||||||
![]() | VIRAL PROTEIN / neuraminidase / influenza / complex / inhibitor | |||||||||
Function / homology | ![]() exo-alpha-(2->3)-sialidase activity / exo-alpha-(2->6)-sialidase activity / exo-alpha-(2->8)-sialidase activity / exo-alpha-sialidase / viral budding from plasma membrane / carbohydrate metabolic process / host cell plasma membrane / virion membrane / membrane / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() ![]() | |||||||||
![]() | Pachl, P. / Pokorna, J. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: DNA-linked inhibitor antibody assay (DIANA) as a new method for screening influenza neuraminidase inhibitors. Authors: Kozisek, M. / Navratil, V. / Rojikova, K. / Pokorna, J. / Berenguer Albinana, C. / Pachl, P. / Zemanova, J. / Machara, A. / Sacha, P. / Hudlicky, J. / Cisarova, I. / Rezacova, P. / Konvalinka, J. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 185.5 KB | Display | ![]() |
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PDB format | ![]() | 147.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.3 MB | Display | ![]() |
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Full document | ![]() | 2.3 MB | Display | |
Data in XML | ![]() | 38.3 KB | Display | |
Data in CIF | ![]() | 58.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 42598.449 Da / Num. of mol.: 2 / Fragment: UNP residues 82-468 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: NA / Production host: ![]() ![]() |
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-Sugars , 4 types, 6 molecules ![](data/chem/img/NAG.gif)
#2: Polysaccharide | Source method: isolated from a genetically manipulated source #3: Polysaccharide | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #4: Polysaccharide | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1- ...alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #7: Sugar | |
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-Non-polymers , 4 types, 837 molecules ![](data/chem/img/CA.gif)
![](data/chem/img/EEW.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/HOH.gif)
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![](data/chem/img/EDO.gif)
![](data/chem/img/HOH.gif)
#5: Chemical | ChemComp-CA / #6: Chemical | #8: Chemical | ChemComp-EDO / #9: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.83 Å3/Da / Density % sol: 56.56 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 8 / Details: 0.1 M HEPES, pH 7.75, 5% PEG 8000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Sep 29, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.918409 Å / Relative weight: 1 |
Reflection | Resolution: 1.61→44.89 Å / Num. obs: 121339 / % possible obs: 98.2 % / Redundancy: 3.13 % / Biso Wilson estimate: 22.4 Å2 / CC1/2: 0.997 / Rrim(I) all: 0.095 / Net I/σ(I): 10.29 |
Reflection shell | Resolution: 1.61→1.71 Å / Redundancy: 2.79 % / Mean I/σ(I) obs: 1.88 / Num. unique obs: 19127 / CC1/2: 0.758 / Rrim(I) all: 0.645 / % possible all: 96.4 |
-Phasing
Phasing | Method: ![]() | ||||||
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Phasing MR | R rigid body: 0.52
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 17.716 Å2
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Refinement step | Cycle: 1 / Resolution: 1.61→44.89 Å
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Refine LS restraints |
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