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Open data
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Basic information
Entry | Database: PDB / ID: 6fya | ||||||
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Title | The crystal structure of EncM under anaerobic conditions | ||||||
![]() | Putative FAD-dependent oxygenase EncM | ||||||
![]() | FLAVOPROTEIN / monooxygenase / flavin-N5-oxide / FAD / EncM / oxygenating species / oxygen binding | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Saleem-Batcha, R. / Teufel, R. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Enzymatic control of dioxygen binding and functionalization of the flavin cofactor. Authors: Saleem-Batcha, R. / Stull, F. / Sanders, J.N. / Moore, B.S. / Palfey, B.A. / Houk, K.N. / Teufel, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 184.9 KB | Display | ![]() |
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PDB format | ![]() | 147.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 34.5 KB | Display | |
Data in CIF | ![]() | 47.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6foqC ![]() 6fowC ![]() 6fp3C ![]() 6fy8C ![]() 6fy9C ![]() 6fybC ![]() 6fycC ![]() 6fydC ![]() 6fyeC ![]() 6fyfC ![]() 6fygC ![]() 4xloS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 50074.371 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.14 Å3/Da / Density % sol: 42.51 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop Details: 0.1M HEPES-Na, 0.2M Calcium Acetate, 20-30% PEG 3350, pH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Feb 17, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→126.62 Å / Num. obs: 26304 / % possible obs: 97.5 % / Redundancy: 4.2 % / Rmerge(I) obs: 0.18 / Net I/σ(I): 4.8 |
Reflection shell | Resolution: 2.6→2.74 Å / Rmerge(I) obs: 0.46 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4XLO Resolution: 2.6→85.399 Å / SU ML: 0.4 / Cross valid method: NONE / σ(F): 1.34 / Phase error: 33.22
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→85.399 Å
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Refine LS restraints |
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LS refinement shell |
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