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- PDB-6fj5: New Insights into the Role of DNA Shape on Its Recognition by p53... -

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Basic information

Entry
Database: PDB / ID: 6fj5
TitleNew Insights into the Role of DNA Shape on Its Recognition by p53 Proteins (complex p53DBD-AGG-HG)
Components
  • Cellular tumor antigen p53
  • DNA
KeywordsTRANSCRIPTION / P53 / TRANSCRIPTION FACTOR / DNA BINDING / DNA RECOGNITION / HOOGSTEEN BASE-PAIRING / TRANSCRIPTION REGULATION / APOPTOSIS / BIOLOGICAL RHYTHMS / CELL CYCLE / NUCLEUS / TUMOR SUPPRESSOR / ANTIGEN NY-CO-13 / PHOSPHOPROTEIN
Function / homology
Function and homology information


Loss of function of TP53 in cancer due to loss of tetramerization ability / Regulation of TP53 Expression / negative regulation of helicase activity / signal transduction by p53 class mediator / negative regulation of G1 to G0 transition / negative regulation of glucose catabolic process to lactate via pyruvate / Transcriptional activation of cell cycle inhibitor p21 / regulation of intrinsic apoptotic signaling pathway by p53 class mediator / negative regulation of pentose-phosphate shunt / ATP-dependent DNA/DNA annealing activity ...Loss of function of TP53 in cancer due to loss of tetramerization ability / Regulation of TP53 Expression / negative regulation of helicase activity / signal transduction by p53 class mediator / negative regulation of G1 to G0 transition / negative regulation of glucose catabolic process to lactate via pyruvate / Transcriptional activation of cell cycle inhibitor p21 / regulation of intrinsic apoptotic signaling pathway by p53 class mediator / negative regulation of pentose-phosphate shunt / ATP-dependent DNA/DNA annealing activity / Activation of NOXA and translocation to mitochondria / regulation of cell cycle G2/M phase transition / regulation of fibroblast apoptotic process / intrinsic apoptotic signaling pathway in response to hypoxia / oligodendrocyte apoptotic process / negative regulation of miRNA processing / positive regulation of thymocyte apoptotic process / oxidative stress-induced premature senescence / regulation of tissue remodeling / glucose catabolic process to lactate via pyruvate / positive regulation of mitochondrial membrane permeability / positive regulation of programmed necrotic cell death / mRNA transcription / bone marrow development / circadian behavior / regulation of mitochondrial membrane permeability involved in apoptotic process / germ cell nucleus / RUNX3 regulates CDKN1A transcription / TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain / TP53 Regulates Transcription of Death Receptors and Ligands / Activation of PUMA and translocation to mitochondria / regulation of DNA damage response, signal transduction by p53 class mediator / histone deacetylase regulator activity / negative regulation of glial cell proliferation / Regulation of TP53 Activity through Association with Co-factors / negative regulation of neuroblast proliferation / T cell lineage commitment / mitochondrial DNA repair / Formation of Senescence-Associated Heterochromatin Foci (SAHF) / ER overload response / B cell lineage commitment / thymocyte apoptotic process / TP53 Regulates Transcription of Caspase Activators and Caspases / negative regulation of mitophagy / cardiac septum morphogenesis / negative regulation of DNA replication / entrainment of circadian clock by photoperiod / PI5P Regulates TP53 Acetylation / negative regulation of telomere maintenance via telomerase / Zygotic genome activation (ZGA) / positive regulation of release of cytochrome c from mitochondria / Association of TriC/CCT with target proteins during biosynthesis / necroptotic process / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / rRNA transcription / TFIID-class transcription factor complex binding / SUMOylation of transcription factors / TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain / intrinsic apoptotic signaling pathway by p53 class mediator / T cell proliferation involved in immune response / negative regulation of reactive oxygen species metabolic process / positive regulation of execution phase of apoptosis / Transcriptional Regulation by VENTX / replicative senescence / cellular response to UV-C / general transcription initiation factor binding / intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress / cellular response to actinomycin D / neuroblast proliferation / positive regulation of RNA polymerase II transcription preinitiation complex assembly / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / response to X-ray / type II interferon-mediated signaling pathway / hematopoietic stem cell differentiation / Pyroptosis / chromosome organization / viral process / embryonic organ development / somitogenesis / TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest / glial cell proliferation / hematopoietic progenitor cell differentiation / core promoter sequence-specific DNA binding / negative regulation of stem cell proliferation / cellular response to glucose starvation / cis-regulatory region sequence-specific DNA binding / mitophagy / negative regulation of fibroblast proliferation / positive regulation of cardiac muscle cell apoptotic process / positive regulation of intrinsic apoptotic signaling pathway / tumor necrosis factor-mediated signaling pathway / negative regulation of proteolysis / Regulation of TP53 Activity through Acetylation / mitotic G1 DNA damage checkpoint signaling / gastrulation / 14-3-3 protein binding / response to salt stress / MDM2/MDM4 family protein binding / cardiac muscle cell apoptotic process / transcription repressor complex
Similarity search - Function
Immunoglobulin-like - #720 / Cellular tumor antigen p53, transactivation domain 2 / Transactivation domain 2 / p53 transactivation domain / P53 transactivation motif / p53 family signature. / p53, tetramerisation domain / P53 tetramerisation motif / p53, DNA-binding domain / P53 DNA-binding domain ...Immunoglobulin-like - #720 / Cellular tumor antigen p53, transactivation domain 2 / Transactivation domain 2 / p53 transactivation domain / P53 transactivation motif / p53 family signature. / p53, tetramerisation domain / P53 tetramerisation motif / p53, DNA-binding domain / P53 DNA-binding domain / p53 tumour suppressor family / p53-like tetramerisation domain superfamily / p53/RUNT-type transcription factor, DNA-binding domain superfamily / p53-like transcription factor, DNA-binding / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
DNA / DNA (> 10) / Cellular tumor antigen p53
Similarity search - Component
Biological speciesHomo sapiens (human)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.051 Å
AuthorsGolovenko, D. / Rozenberg, H. / Shakked, Z.
CitationJournal: Structure / Year: 2018
Title: New Insights into the Role of DNA Shape on Its Recognition by p53 Proteins.
Authors: Golovenko, D. / Brauning, B. / Vyas, P. / Haran, T.E. / Rozenberg, H. / Shakked, Z.
History
DepositionJan 20, 2018Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jun 27, 2018Provider: repository / Type: Initial release
Revision 1.1Jan 2, 2019Group: Data collection / Database references / Structure summary
Category: citation / citation_author / struct
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _struct.title
Revision 1.2Jan 17, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Cellular tumor antigen p53
B: Cellular tumor antigen p53
C: Cellular tumor antigen p53
D: Cellular tumor antigen p53
F: DNA
G: DNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)104,03124
Polymers102,9016
Non-polymers1,13118
Water13,241735
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: native gel electrophoresis
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area12240 Å2
ΔGint-29 kcal/mol
Surface area40960 Å2
MethodPISA
Unit cell
Length a, b, c (Å)68.517, 98.024, 73.171
Angle α, β, γ (deg.)90.000, 93.390, 90.000
Int Tables number4
Space group name H-MP1211

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Components

#1: Protein
Cellular tumor antigen p53 / Antigen NY-CO-13 / Phosphoprotein p53 / Tumor suppressor p53


Mass: 22505.582 Da / Num. of mol.: 4 / Fragment: P53 DNA BINDING DOMAIN, UNP residues 94-293
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TP53, P53 / Plasmid: PET27-B / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P04637
#2: DNA chain DNA


Mass: 6439.159 Da / Num. of mol.: 2 / Fragment: DNA / Source method: obtained synthetically / Details: SYNTHESISED BY IDT / Source: (synth.) synthetic construct (others)
#3: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 4 / Fragment: ZN / Source method: obtained synthetically / Formula: Zn
#4: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 14 / Fragment: 1,2-ETHANEDIOL / Source method: obtained synthetically / Formula: C2H6O2
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 735 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.38 Å3/Da / Density % sol: 48.4 % / Mosaicity: 0.222 °
Crystal growTemperature: 292 K / Method: evaporation / pH: 7
Details: 0.1 M Sodium formate pH 7.0, 12% w/v Polyethylene glycol 3,350

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: ID29 / Wavelength: 0.8856 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 21, 2013
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.8856 Å / Relative weight: 1
ReflectionResolution: 2.05→50 Å / Num. obs: 59807 / % possible obs: 99 % / Observed criterion σ(I): -3 / Redundancy: 4.8 % / Rmerge(I) obs: 0.186 / Rsym value: 0.186 / Net I/av σ(I): 10.087 / Net I/σ(I): 3.1
Reflection shell
Resolution (Å)Redundancy (%)Rmerge(I) obsDiffraction-ID% possible all
2.05-2.094.50.475199.3
2.09-2.124.60.466199
2.12-2.164.50.43199.3
2.16-2.214.60.409199.3
2.21-2.264.40.365198.7
2.26-2.314.30.36197.9
2.31-2.3750.35199.6
2.37-2.435.10.333199.7
2.43-2.55.10.322199.5
2.5-2.585.10.306199.5
2.58-2.685.10.275199.3
2.68-2.7850.253199.1
2.78-2.914.70.226199.1
2.91-3.064.50.193198.1
3.06-3.255.20.173199.7
3.25-3.5150.142199.3
3.51-3.864.80.126198.7
3.86-4.424.30.106196.8
4.42-5.564.80.11199.3
5.56-504.60.108198.3

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Phasing

PhasingMethod: molecular replacement
Phasing MR
Highest resolutionLowest resolution
Rotation2.05 Å49.01 Å
Translation2.05 Å49.01 Å

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Processing

Software
NameVersionClassification
PHENIXrefinement
PHASER2.5.7phasing
HKL-2000data collection
HKL-2000data scaling
PDB_EXTRACT3.2data extraction
HKL-2000data reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1TSR

1tsr


Resolution: 2.051→49.012 Å / SU ML: 0.29 / Cross valid method: FREE R-VALUE / σ(F): 1.41 / Phase error: 32.31
Details: Translational non-crystallographic symmetry (tNCS) correction
RfactorNum. reflection% reflectionSelection details
Rfree0.2438 2001 3.37 %Random selection
Rwork0.1987 ---
obs0.2002 59381 98.17 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 120.41 Å2 / Biso mean: 23.7163 Å2 / Biso min: 4.4 Å2
Refinement stepCycle: final / Resolution: 2.051→49.012 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6103 820 60 735 7718
Biso mean--22.71 29.28 -
Num. residues----836
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0097271
X-RAY DIFFRACTIONf_angle_d1.02810006
X-RAY DIFFRACTIONf_chiral_restr0.061105
X-RAY DIFFRACTIONf_plane_restr0.0071170
X-RAY DIFFRACTIONf_dihedral_angle_d21.0694284
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 14

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.0511-2.10230.30591320.24414057418997
2.1023-2.15920.27361480.23584107425599
2.1592-2.22270.36711500.22734124427499
2.2227-2.29450.25911290.22214065419498
2.2945-2.37650.26011530.21634118427199
2.3765-2.47160.26511420.210341294271100
2.4716-2.58410.26341470.2144131427899
2.5841-2.72030.28391430.20434142428599
2.7203-2.89070.22991550.20574074422999
2.8907-3.11390.24441370.19244115425298
3.1139-3.42720.21741460.1834126427299
3.4272-3.92290.23381390.17234118425798
3.9229-4.94180.19641400.16834114425498
4.9418-49.0260.21021400.19873960410093
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.41770.2193-0.21330.31680.15660.39980.0612-0.1165-0.02590.05460.01660.00470.1486-0.34840.0080.2154-0.02790.03510.3363-0.01870.2031-11.87492.8762-21.3417
20.7039-0.21730.68261.0369-0.26990.6528-0.03170.1684-0.0676-0.1922-0.05030.0166-0.00710.1489-0.13820.20550.17860.02590.05320.12960.1601-6.59273.4809-19.5167
30.16030.02430.25590.4707-0.11380.4692-0.0144-0.0787-0.089-0.1570.07920.2124-0.0313-0.14221.40920.02420.11540.0178-0.0021-0.02460.1219-18.998413.8355-12.3346
40.4795-0.1556-0.06852.11252.21932.34990.1597-0.0370.03690.00070.1678-0.4155-0.26150.282.02460.1867-0.0151-0.0190.05040.01050.1068-8.180822.5693-7.4917
51.9-0.5873-0.16770.2293-0.04360.24640.05710.14110.36780.26680.01360.0138-0.1128-0.03750.40530.12850.01650.03910.04780.01060.1766-11.154120.2573-19.7202
60.89180.11580.75391.1682-0.07360.73590.06870.1170.0269-0.02820.02950.0395-0.03090.14550.00070.1634-0.01040.03060.15620.01730.1719-10.70289.3247-18.2782
70.3927-0.20590.15830.56380.20980.26030.0859-0.14230.0079-0.0845-0.17780.1640.3744-0.0358-0.22780.22450.00230.02640.1409-0.04060.1099-13.9791-7.805-5.369
80.8051-0.14790.06480.9898-0.21560.05610.1750.1548-0.0729-0.49460.02960.0204-0.0172-0.28440.58060.32640.0358-0.07720.18460.00120.132717.32355.5597-28.1564
90.43420.0452-0.09080.21440.20.2275-0.0204-0.15880.09640.1284-0.08890.07730.16440.087-0.79330.17120.10490.12060.2193-0.00910.199631.8228-2.1247-8.6852
100.10280.0079-0.05150.04950.03020.320.0408-0.0343-0.0101-0.0264-0.0315-0.1388-0.03540.3274-0.01980.229-0.0507-0.00420.2165-0.04690.165127.66563.482-19.5076
110.2528-0.2290.12580.45070.03690.73620.0673-0.14710.3046-0.0746-0.0428-0.0002-0.19840.00730.00010.2405-0.01910.02250.3061-0.01340.206921.422617.0358-12.1075
120.06390.0807-0.06180.37560.04570.1114-0.06740.44390.3665-0.3503-0.01910.3181-0.48080.0498-0.00190.31250.0276-0.07230.28280.04790.25616.118822.3439-20.3423
130.6128-0.3224-0.56370.9380.19310.55270.0815-0.0889-0.2789-0.19280.0714-0.11860.17050.16280.07970.16540.0634-0.02630.3029-0.07430.17831.595810.9689-27.3856
140.7756-0.10640.5510.88670.15990.791-0.08110.01950.0858-0.05480.0267-0.08360.0068-0.1051-0.02440.17840.0361-0.02440.16960.00950.116721.42518.888-15.865
150.0425-0.0282-0.03440.56820.02940.2148-0.058-0.0191-0.04660.02950.01420.11230.22740.0184-0.92160.29130.0486-0.07590.09720.15320.354820.2843-7.7988-5.3835
162.27220.6994-0.54612.21160.05440.61660.1078-0.44490.08030.23440.0277-0.5946-0.25270.18550.03360.25190.0441-0.02010.2029-0.06530.22117.63327.747533.407
170.1508-0.143-0.0120.14840.02340.0181-0.0418-0.0191-0.04290.0371-0.00590.07190.0232-0.0115-0.21090.08370.01720.03760.12290.03680.47713.16938.562123.2195
181.4595-0.09960.05181.20030.27090.6946-0.02320.02420.1473-0.00340.0308-0.0601-0.1876-0.0081-0.00010.21420.0059-0.01960.1976-0.01210.231812.557925.922519.2721
190.8331-0.56690.44580.3974-0.3040.23440.0188-0.43420.00420.00930.1855-0.0113-0.1613-0.2246-0.0370.2647-0.0403-0.00310.3189-0.01250.25683.464831.23430.5882
200.53680.36390.3260.8038-0.11040.3625-0.17470.0334-0.1806-0.06880.0914-0.03090.042-0.01030.00120.176-0.0146-0.01080.2142-0.03740.171613.248319.394216.2731
210.4633-0.34510.54180.398-0.15481.1145-0.1297-0.26980.5780.0812-0.12590.0446-0.38770.0304-0.33680.2735-0.06810.03520.2112-0.08680.440215.537532.096528.8707
220.5643-0.2563-0.00930.335-0.11320.34280.0504-0.1591-0.12640.3537-0.03720.02790.1336-0.0375-0.04680.3069-0.03910.04440.1850.02220.241815.021211.582525.2189
230.2027-0.28760.01870.4214-0.02680.00380.0426-0.36560.12310.3138-0.0223-0.1074-0.01360.0827-0.22890.2992-0.1493-0.00160.3908-0.05990.1966-16.617627.729533.4157
240.13140.11230.03120.20330.09460.06510.0294-0.1407-0.00880.1939-0.16470.06890.0575-0.0687-0.58510.2702-0.20660.07080.3001-0.08280.2884-31.05518.569723.2281
251.22860.0273-0.08091.11290.09720.83910.0749-0.02360.0576-0.0386-0.1130.0732-0.0356-0.0504-0.2190.1539-0.03530.00720.11890.00630.1188-21.579823.671919.6336
261.2214-0.11770.45731.2318-0.26891.26450.0045-0.19140.13110.0983-0.0228-0.0080.1209-0.0316-0.06070.0965-0.01430.00330.1356-0.02770.1697-21.897223.568822.9117
270.21120.0197-0.10370.14450.06670.1098-0.0533-0.0407-0.06090.0458-0.1414-0.12650.07370.1403-2.05130.0196-0.09590.14980.04570.11820.0154-17.0240.36189.2185
280.231-0.0933-0.24130.1516-0.00080.30910.0999-0.3093-0.44430.1291-0.15590.16920.15330.1403-0.00240.3462-0.0523-0.1810.38550.07270.551517.23080.35479.2102
290.24990.02530.05030.53240.17110.26240.04640.0476-0.13160.0195-0.0221-0.06530.21010.11520.09790.1950.02340.0618-0.0159-0.05070.095718.21990.19298.8216
300.1410.0014-0.19380.3980.01270.25770.34640.2442-0.1798-0.2465-0.4230.31830.06270.0157-0.00060.3473-0.0238-0.12080.43810.0430.4344-16.04340.19578.8335
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 94 through 123 )A94 - 123
2X-RAY DIFFRACTION2chain 'A' and (resid 124 through 155 )A124 - 155
3X-RAY DIFFRACTION3chain 'A' and (resid 156 through 180 )A156 - 180
4X-RAY DIFFRACTION4chain 'A' and (resid 181 through 194 )A181 - 194
5X-RAY DIFFRACTION5chain 'A' and (resid 195 through 213 )A195 - 213
6X-RAY DIFFRACTION6chain 'A' and (resid 214 through 277 )A214 - 277
7X-RAY DIFFRACTION7chain 'A' and (resid 278 through 292 )A278 - 292
8X-RAY DIFFRACTION8chain 'B' and (resid 94 through 112 )B94 - 112
9X-RAY DIFFRACTION9chain 'B' and (resid 113 through 123 )B113 - 123
10X-RAY DIFFRACTION10chain 'B' and (resid 124 through 155 )B124 - 155
11X-RAY DIFFRACTION11chain 'B' and (resid 156 through 203 )B156 - 203
12X-RAY DIFFRACTION12chain 'B' and (resid 204 through 213 )B204 - 213
13X-RAY DIFFRACTION13chain 'B' and (resid 214 through 228 )B214 - 228
14X-RAY DIFFRACTION14chain 'B' and (resid 229 through 277 )B229 - 277
15X-RAY DIFFRACTION15chain 'B' and (resid 278 through 292 )B278 - 292
16X-RAY DIFFRACTION16chain 'C' and (resid 94 through 113 )C94 - 113
17X-RAY DIFFRACTION17chain 'C' and (resid 114 through 123 )C114 - 123
18X-RAY DIFFRACTION18chain 'C' and (resid 124 through 213 )C124 - 213
19X-RAY DIFFRACTION19chain 'C' and (resid 214 through 229 )C214 - 229
20X-RAY DIFFRACTION20chain 'C' and (resid 230 through 250 )C230 - 250
21X-RAY DIFFRACTION21chain 'C' and (resid 251 through 263 )C251 - 263
22X-RAY DIFFRACTION22chain 'C' and (resid 264 through 292 )C264 - 292
23X-RAY DIFFRACTION23chain 'D' and (resid 94 through 113 )D94 - 113
24X-RAY DIFFRACTION24chain 'D' and (resid 114 through 123 )D114 - 123
25X-RAY DIFFRACTION25chain 'D' and (resid 124 through 194 )D124 - 194
26X-RAY DIFFRACTION26chain 'D' and (resid 195 through 292 )D195 - 292
27X-RAY DIFFRACTION27chain 'F' and (resid 1 through 10 )F1 - 10
28X-RAY DIFFRACTION28chain 'F' and (resid 11 through 20 )F11 - 20
29X-RAY DIFFRACTION29chain 'G' and (resid 1 through 10 )G1 - 10
30X-RAY DIFFRACTION30chain 'G' and (resid 11 through 20 )G11 - 20

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