+Open data
-Basic information
Entry | Database: PDB / ID: 6eyy | ||||||
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Title | Anti-CRISPR AcrIIa6 cubic form | ||||||
Components | AcrIIa6 | ||||||
Keywords | VIRAL PROTEIN / Anti-CRISPR / HTH fold / DNA binding | ||||||
Function / homology | Uncharacterized protein Function and homology information | ||||||
Biological species | Streptococcus phage 73 (virus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | ||||||
Authors | Cambillau, C. / Amigues, B. / Moineau, S. | ||||||
Citation | Journal: Nat Commun / Year: 2018 Title: Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins. Authors: Hynes, A.P. / Rousseau, G.M. / Agudelo, D. / Goulet, A. / Amigues, B. / Loehr, J. / Romero, D.A. / Fremaux, C. / Horvath, P. / Doyon, Y. / Cambillau, C. / Moineau, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6eyy.cif.gz | 164.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6eyy.ent.gz | 132.9 KB | Display | PDB format |
PDBx/mmJSON format | 6eyy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6eyy_validation.pdf.gz | 428.1 KB | Display | wwPDB validaton report |
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Full document | 6eyy_full_validation.pdf.gz | 433.4 KB | Display | |
Data in XML | 6eyy_validation.xml.gz | 18.2 KB | Display | |
Data in CIF | 6eyy_validation.cif.gz | 26.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ey/6eyy ftp://data.pdbj.org/pub/pdb/validation_reports/ey/6eyy | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 21464.391 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptococcus phage 73 (virus) / Variant: Steptococcus thermophilus phage D1811 / Production host: Escherichia coli BL21 (bacteria) / References: UniProt: A0A1S5PRR0*PLUS #2: Water | ChemComp-HOH / | Source details | Streptococcus thermophilus phage D1176 | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 5.23 Å3/Da / Density % sol: 76.46 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.2M Magnesium chloride, 0.1M tris pH6.5-7.5, 5-15%(w/v) PEG 8000. PH range: 6.5 - 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 1 / Wavelength: 0.9789 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Sep 22, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9789 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→50 Å / Num. obs: 32420 / % possible obs: 99.9 % / Redundancy: 26 % / Biso Wilson estimate: 78.08 Å2 / CC1/2: 0.995 / Rrim(I) all: 0.189 / Net I/σ(I): 13.3 |
Reflection shell | Resolution: 2.5→2.65 Å / Redundancy: 26 % / Mean I/σ(I) obs: 2.2 / Num. unique obs: 32420 / CC1/2: 0.91 / Rrim(I) all: 1 / % possible all: 99.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.5→46.84 Å / Cor.coef. Fo:Fc: 0.9288 / Cor.coef. Fo:Fc free: 0.9275 / SU R Cruickshank DPI: 0.239 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.259 / SU Rfree Blow DPI: 0.202 / SU Rfree Cruickshank DPI: 0.195
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Displacement parameters | Biso mean: 64.9 Å2
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Refine analyze | Luzzati coordinate error obs: 0.414 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: 1 / Resolution: 2.5→46.84 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.5→2.58 Å / Total num. of bins used: 16
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Refinement TLS params. | S33: -0.0392 Å ° / Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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