+Open data
-Basic information
Entry | Database: PDB / ID: 6evi | ||||||
---|---|---|---|---|---|---|---|
Title | solution NMR structure of EB1 C terminus (191-260) | ||||||
Components | Microtubule-associated protein RP/EB family member 1 | ||||||
Keywords | PROTEIN BINDING / End-binding protein / EB1 / microtubules / SxIP / Microtubule-associated protein RP/EB family member 1 / MAPRE1 / APC-binding protein EB1 | ||||||
Function / homology | Function and homology information protein localization to astral microtubule / cortical microtubule cytoskeleton / mitotic spindle astral microtubule end / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Resolution of Sister Chromatid Cohesion / The role of GTSE1 in G2/M progression after G2 checkpoint / RHO GTPases Activate Formins / protein localization to microtubule ...protein localization to astral microtubule / cortical microtubule cytoskeleton / mitotic spindle astral microtubule end / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Resolution of Sister Chromatid Cohesion / The role of GTSE1 in G2/M progression after G2 checkpoint / RHO GTPases Activate Formins / protein localization to microtubule / Separation of Sister Chromatids / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of NuMA to mitotic centrosomes / microtubule plus-end / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / cell projection membrane / Regulation of PLK1 Activity at G2/M Transition / attachment of mitotic spindle microtubules to kinetochore / microtubule plus-end binding / non-motile cilium assembly / microtubule bundle formation / protein localization to centrosome / microtubule organizing center / negative regulation of microtubule polymerization / mitotic spindle pole / microtubule polymerization / establishment of mitotic spindle orientation / spindle assembly / regulation of microtubule polymerization or depolymerization / spindle midzone / cytoplasmic microtubule / positive regulation of microtubule polymerization / ciliary basal body / cell projection / microtubule cytoskeleton / cell migration / microtubule / molecular adaptor activity / cell division / focal adhesion / centrosome / protein kinase binding / Golgi apparatus / identical protein binding Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Barsukov, I.L. / Almeida, T.B. | ||||||
Citation | Journal: Sci Rep / Year: 2017 Title: Targeting SxIP-EB1 interaction: An integrated approach to the discovery of small molecule modulators of dynamic binding sites. Authors: Almeida, T.B. / Carnell, A.J. / Barsukov, I.L. / Berry, N.G. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 6evi.cif.gz | 941.3 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb6evi.ent.gz | 811.4 KB | Display | PDB format |
PDBx/mmJSON format | 6evi.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ev/6evi ftp://data.pdbj.org/pub/pdb/validation_reports/ev/6evi | HTTPS FTP |
---|
-Related structure data
Related structure data | 6evqC C: citing same article (ref.) |
---|---|
Similar structure data | |
Other databases |
|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 8049.979 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Mapre1 / Production host: Escherichia coli (E. coli) / References: UniProt: Q61166 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details | Type: solution Contents: 1 mM [U-13C; U-15N] End binding protein 1 (EB1), 90% H2O/10% D2O Label: 13C, 15N / Solvent system: 90% H2O/10% D2O |
---|---|
Sample | Conc.: 1 mM / Component: End binding protein 1 (EB1) / Isotopic labeling: [U-13C; U-15N] |
Sample conditions | Ionic strength: 50 mM / Label: H2O / pH: 6.5 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
|
---|
-Processing
NMR software |
| |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: closest to the average | |||||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 20 / Conformers submitted total number: 20 |