- PDB-6ec0: Crystal structure of the wild-type heterocomplex between coil 1B ... -
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Entry
Database: PDB / ID: 6ec0
Title
Crystal structure of the wild-type heterocomplex between coil 1B domains of human intermediate filament proteins keratin 1 (KRT1) and keratin 10 (KRT10)
positive regulation of epidermis development / structural constituent of skin epidermis / keratin filament / Keratinization / intermediate filament organization / Formation of the cornified envelope / peptide cross-linking / cornified envelope / protein heterotetramerization / intermediate filament ...positive regulation of epidermis development / structural constituent of skin epidermis / keratin filament / Keratinization / intermediate filament organization / Formation of the cornified envelope / peptide cross-linking / cornified envelope / protein heterotetramerization / intermediate filament / epidermis development / keratinocyte differentiation / epithelial cell differentiation / cytoskeleton / protein heterodimerization activity / cell surface / extracellular space / extracellular exosome / membrane / nucleus / plasma membrane / cytoplasm / cytosol Similarity search - Function
Keratin type II cytoskeletal 1, tail / Keratin type II cytoskeletal 1 tail / Keratin, type I / Keratin, type II / Keratin type II head / Keratin type II head / : / Intermediate filament protein, conserved site / Intermediate filament protein / Intermediate filament (IF) rod domain signature. ...Keratin type II cytoskeletal 1, tail / Keratin type II cytoskeletal 1 tail / Keratin, type I / Keratin, type II / Keratin type II head / Keratin type II head / : / Intermediate filament protein, conserved site / Intermediate filament protein / Intermediate filament (IF) rod domain signature. / Intermediate filament, rod domain / Intermediate filament (IF) rod domain profile. / Intermediate filament protein Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 35 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 4.67 Å3/Da / Density % sol: 73.66 % / Description: thin, rod-shaped needles
Crystal grow
Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 11% PEG 3350, 0.1 M HEPES, 5 mM cobalt chloride, 5 mM cadmium chloride, 5 mM magnesium chloride, 5 mM nickel chloride
Method to determine structure: MOLECULAR REPLACEMENT Starting model: Phase problem initially solved using SAD on an isomorphous crystal whose data was collected at the cadmium edge (8500eV). This structure was used as starting model in MolRep to obtain ...Starting model: Phase problem initially solved using SAD on an isomorphous crystal whose data was collected at the cadmium edge (8500eV). This structure was used as starting model in MolRep to obtain the higher resolution KRT1/KRT10 1B structure. Resolution: 2.983→46.196 Å / SU ML: 0.72 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 46.21
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