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- PDB-6eb4: OhrB (Organic Hydroperoxide Resistance protein) from Chromobacter... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6eb4 | ||||||
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Title | OhrB (Organic Hydroperoxide Resistance protein) from Chromobacterium violaceum | ||||||
![]() | Organic hydroperoxide resistance protein | ||||||
![]() | OXIDOREDUCTASE / Ohr / thiol-proxidase | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Domingos, R.M. / Teixeira, R.D. / Alegria, T.G.P. / Vieira, P.S. / Murakami, M.T. / Netto, L.E.S. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Substrate and product-assisted catalysis: molecular aspects behind structural switches along Organic Hydroperoxide Resistance Protein catalytic cycle Authors: Domingos, R.M. / Teixeira, R.D. / Zeida, A. / Agudelo, W.A. / Alegria, T.G.P. / da Silva Neto, J.F. / Vieira, P.S. / Murakami, M.T. / Farah, C.S. / Estrin, D.A. / Netto, L.E.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 223.9 KB | Display | ![]() |
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PDB format | ![]() | 181 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 465.1 KB | Display | ![]() |
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Full document | ![]() | 473.9 KB | Display | |
Data in XML | ![]() | 23.4 KB | Display | |
Data in CIF | ![]() | 31.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6ebcC ![]() 6ebdC ![]() 6ebgC ![]() 6ecyC ![]() 6ed0C ![]() 1n2fS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: _ / Beg auth comp-ID: SER / Beg label comp-ID: SER / Refine code: _
NCS ensembles :
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Components
#1: Protein | Mass: 16852.906 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-PEG / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.92 Å3/Da / Density % sol: 63.49 % |
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Crystal grow | Temperature: 291.15 K / Method: vapor diffusion, hanging drop / pH: 7.4 Details: Purified protein at 10mg/ml diluted in 5mM Tris-Hcl pH 7.4 Crystallisation condition: Magnesium Clhoride 200mM, BIS-TRIS 100mM pH 5.5, PEG 3,350 25%w/v |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Oct 13, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.45866 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→46.8 Å / Num. obs: 43780 / % possible obs: 97.5 % / Redundancy: 14.3 % / CC1/2: 0.99 / Rmerge(I) obs: 0.07 / Rpim(I) all: 0.03 / Rrim(I) all: 0.08 / Net I/σ(I): 22.5 |
Reflection shell | Resolution: 2.1→2.16 Å / Redundancy: 8.5 % / Rmerge(I) obs: 2.61 / Mean I/σ(I) obs: 0.9 / Num. unique obs: 3282 / CC1/2: 0.37 / Rpim(I) all: 1.16 / Rrim(I) all: 2.46 / % possible all: 89.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1N2F Resolution: 2.1→35 Å / Cor.coef. Fo:Fc: 0.964 / Cor.coef. Fo:Fc free: 0.952 / SU B: 11.009 / SU ML: 0.137 / Cross valid method: THROUGHOUT / ESU R: 0.173 / ESU R Free: 0.153 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 54.888 Å2
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Refinement step | Cycle: 1 / Resolution: 2.1→35 Å
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Refine LS restraints |
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