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Open data
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Basic information
Entry | Database: PDB / ID: 6e4o | ||||||
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Title | Structure of apo T. brucei RRM: P4(1)2(1)2 form | ||||||
![]() | RNA-binding protein, putative | ||||||
![]() | RNA BINDING PROTEIN / RRM / RNA binding | ||||||
Function / homology | ![]() mitochondrial mRNA processing / mitochondrial mRNA editing complex / cytoplasmic side of mitochondrial outer membrane / kinetoplast / RNA processing / mRNA binding / mitochondrion / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Schumacher, M.A. | ||||||
![]() | ![]() Title: The RRM of the kRNA-editing protein TbRGG2 uses multiple surfaces to bind and remodel RNA. Authors: Travis, B. / Shaw, P.L.R. / Liu, B. / Ravindra, K. / Iliff, H. / Al-Hashimi, H.M. / Schumacher, M.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 125.7 KB | Display | ![]() |
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PDB format | ![]() | 97.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6e4nSC ![]() 6e4pC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 7798.736 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.47 Å3/Da / Density % sol: 50.26 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / Details: 1.2-1.4 M trisodium citrate, HEPES buffer |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210r / Detector: CCD / Date: Feb 24, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→38.676 Å / Num. obs: 28420 / % possible obs: 96 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 2.6 % / CC1/2: 0.997 / Rpim(I) all: 0.035 / Rsym value: 0.05 / Net I/σ(I): 12.5 |
Reflection shell | Resolution: 1.8→1.97 Å / Redundancy: 1.4 % / Mean I/σ(I) obs: 2.3 / CC1/2: 0.977 / Rpim(I) all: 0.208 / Rsym value: 0.212 / % possible all: 80.3 |
-Phasing
Phasing | Method: ![]() |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6E4N Resolution: 1.8→38.676 Å / SU ML: 0.19 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 18.34
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Solvent computation | Shrinkage radii: 0.72 Å / VDW probe radii: 1 Å / Bsol: 40.113 Å2 / ksol: 0.389 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 89.64 Å2 / Biso mean: 23.49 Å2 / Biso min: 6.07 Å2
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Refinement step | Cycle: final / Resolution: 1.8→38.676 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 10
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Refinement TLS params. | Method: refined / Origin x: 23.5625 Å / Origin y: -4.4708 Å / Origin z: -0.5355 Å
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Refinement TLS group |
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