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- PDB-6chv: Proteus vulgaris HigA antitoxin bound to DNA -

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Basic information

Entry
Database: PDB / ID: 6chv
TitleProteus vulgaris HigA antitoxin bound to DNA
Components
  • Antitoxin HigA
  • pHigCryst3
  • pHigCryst4
KeywordsANTITOXIN/DNA / Helix-turn-helix DNA binding protein / DNA-protein complex / toxin-antitoxin systems / ANTITOXIN / ANTITOXIN-DNA complex
Function / homology
Function and homology information


toxin sequestering activity / transcription cis-regulatory region binding / DNA-binding transcription factor activity / protein heterodimerization activity / regulation of DNA-templated transcription
Similarity search - Function
Toxin-antitoxin system, antidote protein, HigA / Helix-turn-helix / Helix-turn-helix XRE-family like proteins / Cro/C1-type HTH domain profile. / lambda repressor-like DNA-binding domains / Cro/C1-type helix-turn-helix domain / 434 Repressor (Amino-terminal Domain) / Lambda repressor-like, DNA-binding domain superfamily / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
DNA / DNA (> 10) / Antitoxin HigA
Similarity search - Component
Biological speciesProteus vulgaris (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.9 Å
AuthorsSchureck, M.A. / Hoffer, E.D. / Onuoha, N. / Dunham, C.M.
CitationJournal: Mol.Microbiol. / Year: 2019
Title: Structural basis of transcriptional regulation by the HigA antitoxin.
Authors: Schureck, M.A. / Meisner, J. / Hoffer, E.D. / Wang, D. / Onuoha, N. / Ei Cho, S. / Lollar 3rd, P. / Dunham, C.M.
History
DepositionFeb 23, 2018Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 27, 2019Provider: repository / Type: Initial release
Revision 1.1Mar 13, 2019Group: Data collection / Database references
Category: citation / citation_author ...citation / citation_author / pdbx_database_proc / pdbx_seq_map_depositor_info
Item: _citation.journal_abbrev / _citation.pdbx_database_id_DOI ..._citation.journal_abbrev / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.name / _pdbx_seq_map_depositor_info.one_letter_code_mod
Revision 1.2Apr 17, 2019Group: Author supporting evidence / Data collection / Category: pdbx_audit_support
Revision 1.3Jun 26, 2019Group: Data collection / Database references / Category: citation / citation_author
Item: _citation.journal_abbrev / _citation.journal_volume ..._citation.journal_abbrev / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.name
Revision 1.4Oct 4, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Antitoxin HigA
C: Antitoxin HigA
I: pHigCryst3
J: pHigCryst4
B: Antitoxin HigA
D: Antitoxin HigA
E: pHigCryst3
F: pHigCryst4
G: Antitoxin HigA
H: Antitoxin HigA
K: pHigCryst3
L: pHigCryst4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)118,07618
Polymers117,93012
Non-polymers1466
Water1,78399
1
A: Antitoxin HigA
C: Antitoxin HigA
I: pHigCryst3
J: pHigCryst4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)39,3345
Polymers39,3104
Non-polymers241
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Antitoxin HigA
D: Antitoxin HigA
E: pHigCryst3
F: pHigCryst4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)39,3345
Polymers39,3104
Non-polymers241
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
G: Antitoxin HigA
H: Antitoxin HigA
K: pHigCryst3
L: pHigCryst4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)39,4078
Polymers39,3104
Non-polymers974
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)79.449, 79.449, 399.427
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number170
Space group name H-MP65

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Components

#1: Protein
Antitoxin HigA / Antidote protein / Host inhibition of growth protein A


Mass: 13213.810 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Proteus vulgaris (bacteria) / Gene: higA / Production host: Escherichia coli (E. coli) / References: UniProt: Q7A224
#2: DNA chain pHigCryst3


Mass: 6390.175 Da / Num. of mol.: 3 / Source method: obtained synthetically / Source: (synth.) Proteus vulgaris (bacteria)
#3: DNA chain pHigCryst4


Mass: 6492.219 Da / Num. of mol.: 3 / Source method: obtained synthetically / Source: (synth.) Proteus vulgaris (bacteria)
#4: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: Mg
#5: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 99 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.37 Å3/Da / Density % sol: 63.51 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.2 M CaCl2 and 10-25% w/v PEG 3,350

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: RAYONIX MX300-HS / Detector: CCD / Date: Feb 22, 2016
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.9→40.41 Å / Num. obs: 76031 / % possible obs: 100 % / Redundancy: 13.8 % / Biso Wilson estimate: 61 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.127 / Rpim(I) all: 0.035 / Rrim(I) all: 0.132 / Net I/σ(I): 11.1
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique obsCC1/2Rpim(I) allRrim(I) all% possible all
2.9-3.0613.80.38946730.9870.1090.404100
9.17-40.4112.80.06210200.9990.0190.06598.9

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Processing

Software
NameVersionClassification
xia20.5.474data reduction
DIALS1.8.1data reduction
Aimless0.6.2data scaling
PHENIXphasing
PHASERphasing
PHENIX1.13-2998refinement
Coot0.8.9model building
PDB_EXTRACT3.24data extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4MCT

4mct


Resolution: 2.9→39.724 Å / SU ML: 0.39 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 34.96
RfactorNum. reflection% reflection
Rfree0.2601 1588 4.94 %
Rwork0.2248 --
obs0.2265 76031 98.21 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 178.97 Å2 / Biso mean: 91.3467 Å2 / Biso min: 35.74 Å2
Refinement stepCycle: final / Resolution: 2.9→39.724 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4074 2565 6 99 6744
Biso mean--87.86 63.47 -
Num. residues----659
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 27

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.7001-2.73430.37251440.3992720286498
2.7343-2.77020.45241340.36732615274998
2.7702-2.80820.35011400.36852701284198
2.8082-2.84830.34531480.35392671281999
2.8483-2.89080.32091190.33972705282499
2.8908-2.93590.36911690.33812705287498
2.9359-2.98410.36881160.31642671278799
2.9841-3.03550.35891390.30972638277798
3.0355-3.09070.30261280.32442727285598
3.0907-3.15010.38141590.29392623278298
3.1501-3.21440.32651380.30822607274597
3.2144-3.28420.35141960.27242598279496
3.2842-3.36060.26321610.24122522268395
3.3606-3.44460.22791270.24312751287897
3.4446-3.53770.3371940.2482646274099
3.5377-3.64170.30911480.25792658280696
3.6417-3.75910.29411220.23752645276797
3.7591-3.89340.2951600.24242634279499
3.8934-4.04910.26211580.20882679283799
4.0491-4.23320.19731240.180127332857100
4.2332-4.45610.2471640.18662701286599
4.4561-4.73490.22051060.18392730283699
4.7349-5.09980.2381620.18872686284899
5.0998-5.61170.24111290.182127272856100
5.6117-6.42080.16541280.174427082836100
6.4208-8.07830.1761200.190927962916100
8.0783-39.72860.20761230.17212678280198
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
17.1863-1.951-0.09285.8402-0.27132.74150.36751.39750.3422-0.1093-0.2588-0.780.28461.761-0.22621.0450.03490.2220.9621-0.05740.721216.2074-34.1175-60.6546
25.4082-4.66165.39876.8014-4.71175.39910.62182.3787-0.9316-2.1192-0.4325-0.1591.55631.303-0.19771.08370.08990.09660.9518-0.00980.57077.8245-36.7814-73.1172
30.70771.405-0.90813.2175-0.58976.9850.38171.33010.5053-0.4994-0.0221-0.12190.0185-0.8809-0.25510.94130.1870.13051.14650.12610.49713.6582-25.8608-71.9493
45.09383.0866-1.13243.3603-3.65327.77010.68321.37190.0753-0.43880.49630.61640.4047-0.9897-0.83850.7918-0.0636-0.00661.30320.08020.3484-4.3532-33.2718-67.6554
58.275-0.93046.34654.8137-0.25396.0140.82680.3371-0.4447-0.0884-0.1003-0.35970.89760.0606-0.60280.92840.06440.08710.7209-0.06550.39494.5169-35.6854-56.1854
68.6204-0.792-5.2466.1426-0.84873.54250.1739-1.2887-0.26091.46990.2366-0.0540.82131.0422-0.43281.19420.1171-0.16640.8027-0.1710.53876.0686-35.4309-35.3627
78.73415.74775.56756.60765.5614.83180.4614-0.4889-0.00131.766-0.56380.74541.1681-0.6213-0.41591.7214-0.2780.36521.1630.06650.9365-16.4602-35.1736-50.4766
89.4425-0.6741-1.74521.833-1.0076.6698-0.0132-0.67820.02820.6525-0.13720.15220.2169-0.68330.08241.1257-0.00720.19220.65020.00250.4341-6.8702-29.498-36.6622
97.7223-5.5965-3.41748.13315.66964.03580.3619-0.7525-0.22580.36180.0410.47810.3010.4891-0.35820.75870.0784-0.02780.5191-0.07360.32521.1242-35.3934-41.4904
102.60280.2969-1.36822.15711.95723.59020.65161.05970.07850.0302-0.33560.52960.3962-1.7352-0.30650.80310.0040.03271.09790.09040.513-8.5511-32.978-59.6079
110.70260.52790.40381.8649-1.68422.81090.24640.75340.7034-0.3540.09780.1835-0.7704-0.3187-0.31411.28990.05350.3991.34250.25550.574310.935-20.8949-77.7186
122.55321.7423-0.18643.44660.23450.06590.68370.63411.148-0.13920.6482-0.0275-1.6461-0.367-0.6081.82860.11920.55941.00330.15230.8151.4719-13.7293-55.2148
137.25430.4534-0.74073.4297-2.26932.080.6336-1.03591.76461.40730.46960.5139-1.8152-0.5552-0.99942.06260.18880.55771.0975-0.13630.9153-8.2378-12.1142-30.4694
143.7273-1.5772.56453.062-1.11813.7570.5179-0.12850.5137-0.0628-0.1282-0.15060.55310.1612-0.3591.35380.05980.3231.0641-0.04550.5609-10.6455-20.2464-30.3577
159.3112-2.75012.36517.63-0.02764.1794-1.07390.80620.6321-0.02361.2949-0.8282-1.7749-0.4933-0.06442.1990.01970.60660.92350.0011.0474-1.6273-9.8525-44.1655
162.96250.5228-2.3616.3697-3.12523.07721.2945-0.01281.26390.5624-0.11250.2444-1.7946-0.6365-1.00941.41660.17870.36921.05280.07860.69083.4078-17.7875-60.0511
178.7129-1.9248-3.88753.64764.74956.46140.53610.57121.6176-0.88170.973-0.6373-1.55630.5453-0.8511.90920.04560.60681.36820.24310.929210.8101-12.3991-76.9059
182.6412.8515-3.99177.9813-4.60736.32910.76480.0632-0.0389-0.47470.4620.68591.5148-0.4422-0.83110.96520.2183-0.11960.74230.0190.42175.1061-56.3429-55.4373
191.29872.1709-0.58437.18853.59146.1799-0.14081.0897-0.3426-0.53710.6906-0.6532-0.42331.2956-0.37560.83940.331-0.12371.08-0.18890.394415.8604-52.8793-65.2621
201.0556-1.70470.13265.03372.56053.36760.2127-0.3418-0.15980.60440.1795-0.48711.10181.0397-0.2150.79650.2784-0.1410.6839-0.16210.430318.1363-58.0946-57.1452
216.817-5.4041-2.09169.24060.84640.78280.1882-0.1974-0.26830.67440.1579-0.99481.19030.5529-0.53780.97290.3314-0.15810.6085-0.10240.453715.997-57.5664-49.3324
224.2581-2.1267-5.25287.32274.57297.14940.83460.1822-0.0008-0.5529-0.1524-0.14120.29010.5387-0.78790.73590.0799-0.0740.6291-0.0960.332313.8464-45.7984-54.2497
235.5158-1.66692.00279.7274-6.63844.8-0.0401-0.263-0.51380.72340.8483-0.33440.3506-1.3424-0.94891.01780.1873-0.17470.9759-0.09680.43978.5771-52.056-43.5719
247.255-2.813-3.03626.91625.83728.56250.2644-0.5732-0.5861.1393-0.81170.51911.4523-1.47120.3391.2673-0.1441-0.12180.96780.09210.58768.6094-56.491-23.7697
253.226-0.336-4.00422.74412.79087.89070.326-0.61420.98670.2472-0.6843-0.1272-1.99210.26230.01491.36530.152-0.18640.9175-0.14170.614717.849-38.4271-26.3157
266.72411.55490.21614.0653-0.0541.48370.2569-0.59-0.05450.7239-0.2952-0.470.5151-0.2868-0.08451.08750.0632-0.20990.8095-0.11590.468921.8023-49.7684-23.533
274.37130.2733.06866.7285-4.23235.0630.7001-1.6499-0.07231.07310.45570.21750.6191-0.7247-0.87320.89650.0185-0.09440.8691-0.04010.488111.1978-50.8685-27.9156
283.94630.9823-1.02851.3222-2.55295.27580.04490.3890.57290.39030.2683-0.1287-0.5562-0.3402-0.29221.19480.2522-0.04930.6899-0.12770.4214.4053-43.2296-37.7677
298.70211.74274.13651.9419-1.54178.0440.6962-1.01061.50920.7633-0.47-0.5114-1.44750.5472-0.29870.83460.03680.08411.0122-0.13910.609521.6675-39.8306-54.8919
308.3467-4.82162.60143.7804-0.33422.72820.60143.2397-0.7304-2.93670.43372.08111.1492-1.1142-1.23321.3108-0.1262-0.22321.43410.04360.94097.2802-44.7318-67.8234
311.05070.94490.69761.12380.09473.09490.6362-0.027-0.44980.33170.7955-0.77871.22241.27490.12531.55540.6279-0.40381.3869-0.52940.633124.5101-64.6827-57.9431
323.7221-1.1701-1.45824.43671.71620.98060.8660.0655-0.25610.19770.1962-1.04590.36781.01180.05791.55880.1894-0.45031.2169-0.29590.677232.8006-54.3086-25.9275
331.82730.42420.87720.9679-0.55411.10590.0814-0.4402-0.18510.03360.1685-0.49860.50820.00760.11631.94520.1599-0.72481.0133-0.2695-0.171630.5632-47.6861-17.6765
342.9704-0.28980.70441.16261.08934.38270.8036-0.1409-0.40920.44340.4542-0.60610.69780.67710.74031.64020.5651-0.6261.391-0.45490.751729.888-59.6032-40.0445
352.83591.53382.37361.63861.09272.04380.70620.1661-0.7637-0.43710.191-0.56431.2250.8783-0.09861.69330.6399-0.45491.522-0.65830.859225.3391-68.2668-64.7812
363.10150.2903-3.07863.2597-1.42893.57230.45760.7940.0761-1.1182-0.6656-0.09530.21131.34870.20511.12930.1518-0.13021.6180.23480.5746-16.633-50.962-85.5291
373.5710.7195-1.78535.5674.29899.70020.15131.02730.3358-0.57080.2982-0.4304-0.03382.0366-0.26120.9190.1657-0.05661.61010.29250.6032-9.9509-52.8996-74.6912
388.48050.1727-1.41578.11795.30466.87870.294-1.46680.5190.94210.28220.22970.21150.7503-0.49910.8327-0.127-0.01190.67350.11290.7521-6.4483-48.0954-46.963
393.9895-1.9092.43625.8065-1.40581.50551.2242-0.5216-0.6520.539-0.7151-0.5333.68421-0.39861.3867-0.0531-0.22210.96570.17650.6084-4.0306-65.0336-52.7595
406.8321-1.2429-0.82916.6966-0.50430.23070.1476-0.1296-0.06720.5946-0.08530.14230.7514-0.636-0.21920.9179-0.1378-0.1580.93110.20260.487-14.1761-60.2983-50.414
411.99612.1682-3.74487.9382-1.17429.02540.44580.10850.34870.66860.12970.33340.47970.1738-0.54260.6464-0.1101-0.05180.59060.16190.3854-7.9481-52.2487-56.1017
424.79765.28444.75976.32275.65545.16280.36351.6774-0.5192-0.53280.358-0.42791.35021.7402-0.82991.08550.1784-0.21311.45030.06270.5834-6.1995-59.481-65.6887
438.34555.154-5.58788.7205-6.57545.4887-0.92260.1595-0.2747-1.00590.4667-0.14182.54880.33380.46381.55190.2606-0.13471.2052-0.18720.8571-10.6906-65.9995-83.892
442.50461.2309-1.06692.36552.00034.25590.9421-0.1191-0.8626-0.4553-0.4050.99740.2865-0.1579-0.28641.16240.0882-0.34461.67590.35550.8564-31.0336-52.9846-83.4266
453.5043-0.4503-1.26175.7265-2.81772.05250.6628-0.4032-0.81520.49750.41960.89250.9637-1.1386-0.94831.3956-0.3984-0.22941.41760.39940.8546-25.3509-64.5829-51.1445
466.5067-1.91635.02860.6959-0.82917.37640.8803-0.7299-0.98620.03950.33410.55790.2115-0.9137-1.0251.3264-0.2657-0.27541.1280.30870.7139-17.997-67.7762-43.7668
471.69310.1397-0.44944.6327-3.62913.66370.9936-0.2095-0.58190.31010.35321.14010.9963-1.9332-1.23981.4124-0.296-0.22961.67080.43380.8863-29.0315-59.2132-65.1344
486.25241.1035-2.65647.80851.82633.79121.1121.1862-0.3708-1.33660.72662.33310.3298-1.2344-1.42281.3495-0.0573-0.4991.71230.42641.0972-34.9504-51.5403-89.9908
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 4 through 9 )A4 - 9
2X-RAY DIFFRACTION2chain 'A' and (resid 10 through 20 )A10 - 20
3X-RAY DIFFRACTION3chain 'A' and (resid 21 through 49 )A21 - 49
4X-RAY DIFFRACTION4chain 'A' and (resid 50 through 63 )A50 - 63
5X-RAY DIFFRACTION5chain 'A' and (resid 64 through 81 )A64 - 81
6X-RAY DIFFRACTION6chain 'A' and (resid 82 through 92 )A82 - 92
7X-RAY DIFFRACTION7chain 'C' and (resid 4 through 8 )C4 - 8
8X-RAY DIFFRACTION8chain 'C' and (resid 9 through 49 )C9 - 49
9X-RAY DIFFRACTION9chain 'C' and (resid 50 through 63 )C50 - 63
10X-RAY DIFFRACTION10chain 'C' and (resid 64 through 91 )C64 - 91
11X-RAY DIFFRACTION11chain 'I' and (resid 1 through 5 )I1 - 5
12X-RAY DIFFRACTION12chain 'I' and (resid 6 through 15 )I6 - 15
13X-RAY DIFFRACTION13chain 'I' and (resid 16 through 21 )I16 - 21
14X-RAY DIFFRACTION14chain 'J' and (resid 1 through 5 )J1 - 5
15X-RAY DIFFRACTION15chain 'J' and (resid 6 through 10 )J6 - 10
16X-RAY DIFFRACTION16chain 'J' and (resid 11 through 15 )J11 - 15
17X-RAY DIFFRACTION17chain 'J' and (resid 16 through 21 )J16 - 21
18X-RAY DIFFRACTION18chain 'B' and (resid 5 through 20 )B5 - 20
19X-RAY DIFFRACTION19chain 'B' and (resid 21 through 31 )B21 - 31
20X-RAY DIFFRACTION20chain 'B' and (resid 32 through 43 )B32 - 43
21X-RAY DIFFRACTION21chain 'B' and (resid 44 through 49 )B44 - 49
22X-RAY DIFFRACTION22chain 'B' and (resid 50 through 63 )B50 - 63
23X-RAY DIFFRACTION23chain 'B' and (resid 64 through 79 )B64 - 79
24X-RAY DIFFRACTION24chain 'B' and (resid 80 through 92 )B80 - 92
25X-RAY DIFFRACTION25chain 'D' and (resid 5 through 20 )D5 - 20
26X-RAY DIFFRACTION26chain 'D' and (resid 21 through 49 )D21 - 49
27X-RAY DIFFRACTION27chain 'D' and (resid 50 through 63 )D50 - 63
28X-RAY DIFFRACTION28chain 'D' and (resid 64 through 78 )D64 - 78
29X-RAY DIFFRACTION29chain 'D' and (resid 79 through 88 )D79 - 88
30X-RAY DIFFRACTION30chain 'D' and (resid 89 through 95 )D89 - 95
31X-RAY DIFFRACTION31chain 'E' and (resid 1 through 10 )E1 - 10
32X-RAY DIFFRACTION32chain 'E' and (resid 11 through 21 )E11 - 21
33X-RAY DIFFRACTION33chain 'F' and (resid 1 through 5 )F1 - 5
34X-RAY DIFFRACTION34chain 'F' and (resid 6 through 15 )F6 - 15
35X-RAY DIFFRACTION35chain 'F' and (resid 16 through 21 )F16 - 21
36X-RAY DIFFRACTION36chain 'G' and (resid 6 through 49 )G6 - 49
37X-RAY DIFFRACTION37chain 'G' and (resid 50 through 81 )G50 - 81
38X-RAY DIFFRACTION38chain 'G' and (resid 82 through 96 )G82 - 96
39X-RAY DIFFRACTION39chain 'H' and (resid 7 through 20 )H7 - 20
40X-RAY DIFFRACTION40chain 'H' and (resid 21 through 49 )H21 - 49
41X-RAY DIFFRACTION41chain 'H' and (resid 50 through 63 )H50 - 63
42X-RAY DIFFRACTION42chain 'H' and (resid 64 through 77 )H64 - 77
43X-RAY DIFFRACTION43chain 'H' and (resid 78 through 92 )H78 - 92
44X-RAY DIFFRACTION44chain 'K' and (resid 1 through 10 )K1 - 10
45X-RAY DIFFRACTION45chain 'K' and (resid 11 through 21 )K11 - 21
46X-RAY DIFFRACTION46chain 'L' and (resid 1 through 5 )L1 - 5
47X-RAY DIFFRACTION47chain 'L' and (resid 6 through 15 )L6 - 15
48X-RAY DIFFRACTION48chain 'L' and (resid 16 through 21 )L16 - 21

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