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- PDB-6bzx: Structure of the artificial complex alpha-Rep/Octarellin V.1 crys... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6bzx | ||||||
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Title | Structure of the artificial complex alpha-Rep/Octarellin V.1 crystallized by counter diffusion in a capillary | ||||||
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![]() | DE NOVO PROTEIN / protein design / artificial protein | ||||||
Biological species | synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Aedo, F. / Contreras-Martel, C. / Martinez-Oyanedel, J. / Bunster, M. / Minard, P. / Van de Weerdt, C. / Figueroa, M. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystallization of the artificial complex alpha-Rep/Octarellin V.1 by counter diffusion allowed to have a most complete structure Authors: Aedo, F. / Figueroa, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 202.5 KB | Display | ![]() |
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PDB format | ![]() | 162.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4zv6S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 32362.977 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: ![]() ![]() |
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#2: Protein | Mass: 24867.068 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: ![]() ![]() |
#3: Chemical | ChemComp-NA / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.98 Å3/Da / Density % sol: 37.8 % |
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Crystal grow | Temperature: 291 K / Method: liquid diffusion / pH: 8 / Details: HEPES 0.1 M, MgCl 0.2 M, PEG 400 25% |
-Data collection
Diffraction | Mean temperature: 273 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Nov 20, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→45.14 Å / Num. obs: 8115 / % possible obs: 96.3 % / Redundancy: 3.7 % / CC1/2: 1 / Rrim(I) all: 0.108 / Net I/σ(I): 25.42 |
Reflection shell | Resolution: 3.1→3.29 Å |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4zv6 Resolution: 3.107→38.941 Å / SU ML: 0.63 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 37.81
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.107→38.941 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -0.2954 Å / Origin y: 0.4091 Å / Origin z: -20.9493 Å
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Refinement TLS group | Selection details: all |