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- PDB-6bxa: Crystal structure of N-terminal fragment of Zebrafish Toll-Like R... -

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Basic information

Entry
Database: PDB / ID: 6bxa
TitleCrystal structure of N-terminal fragment of Zebrafish Toll-Like Receptor 5 (TLR5) with Lamprey Variable Lymphocyte Receptor 2 (VLR2) bound
Components
  • Toll-like receptor 5b, Variable lymphocyte receptor B chimera
  • Variable Lymphocyte Receptor 2
KeywordsIMMUNE SYSTEM / VLR / LEUCINE-RICH REPEAT / antibody
Function / homology
Function and homology information


toll-like receptor 5 signaling pathway / activation of immune response / toll-like receptor signaling pathway / transmembrane signaling receptor activity / signaling receptor activity / inflammatory response / innate immune response / membrane / plasma membrane
Similarity search - Function
Variable lymphocyte receptor, C-terminal / Domain of unknown function (DUF3439) / : / Toll-like receptor / Leucine rich repeat N-terminal domain / Leucine-rich repeat N-terminal domain / Leucine rich repeat N-terminal domain / TIR domain / Cysteine-rich flanking region, C-terminal / Leucine rich repeat C-terminal domain ...Variable lymphocyte receptor, C-terminal / Domain of unknown function (DUF3439) / : / Toll-like receptor / Leucine rich repeat N-terminal domain / Leucine-rich repeat N-terminal domain / Leucine rich repeat N-terminal domain / TIR domain / Cysteine-rich flanking region, C-terminal / Leucine rich repeat C-terminal domain / Toll - interleukin 1 - resistance / TIR domain profile. / Leucine-rich repeat, SDS22-like subfamily / Toll/interleukin-1 receptor homology (TIR) domain / Toll/interleukin-1 receptor homology (TIR) domain superfamily / Leucine rich repeat / Leucine-rich repeat, typical subtype / Leucine-rich repeats, typical (most populated) subfamily / Leucine-rich repeat profile. / Leucine-rich repeat / Leucine-rich repeat domain superfamily
Similarity search - Domain/homology
Toll-like receptor 5b / Variable lymphocyte receptor B
Similarity search - Component
Biological speciesDanio rerio (zebrafish)
Eptatretus burgeri (inshore hagfish)
Petromyzon marinus (sea lamprey)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.298 Å
AuthorsGunn, R.J. / Wilson, I.A. / Cooper, M.D. / Herrin, B.R.
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)A1042266 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI072435 United States
CitationJournal: J. Mol. Biol. / Year: 2018
Title: VLR Recognition of TLR5 Expands the Molecular Characterization of Protein Antigen Binding by Non-Ig-based Antibodies.
Authors: Gunn, R.J. / Herrin, B.R. / Acharya, S. / Cooper, M.D. / Wilson, I.A.
History
DepositionDec 18, 2017Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 9, 2018Provider: repository / Type: Initial release
Revision 1.1Feb 20, 2019Group: Author supporting evidence / Data collection / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.2Dec 18, 2019Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 2.0Jul 29, 2020Group: Atomic model / Data collection ...Atomic model / Data collection / Derived calculations / Structure summary
Category: atom_site / chem_comp ...atom_site / chem_comp / entity / pdbx_branch_scheme / pdbx_chem_comp_identifier / pdbx_entity_branch / pdbx_entity_branch_descriptor / pdbx_entity_branch_link / pdbx_entity_branch_list / pdbx_entity_nonpoly / pdbx_nonpoly_scheme / pdbx_struct_assembly_gen / pdbx_struct_special_symmetry / struct_asym / struct_conn / struct_site / struct_site_gen
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_asym_id / _atom_site.auth_atom_id / _atom_site.auth_comp_id / _atom_site.auth_seq_id / _atom_site.label_asym_id / _atom_site.label_atom_id / _atom_site.label_comp_id / _atom_site.label_entity_id / _atom_site.type_symbol / _chem_comp.name / _chem_comp.type / _pdbx_entity_nonpoly.entity_id / _pdbx_entity_nonpoly.name / _pdbx_struct_assembly_gen.asym_id_list / _pdbx_struct_special_symmetry.label_asym_id / _struct_conn.pdbx_role / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 2.1Mar 23, 2022Group: Author supporting evidence / Database references / Structure summary
Category: chem_comp / database_2 / pdbx_audit_support
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI ..._chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_audit_support.funding_organization
Revision 2.2Oct 4, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 2.3Oct 16, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Toll-like receptor 5b, Variable lymphocyte receptor B chimera
B: Toll-like receptor 5b, Variable lymphocyte receptor B chimera
C: Variable Lymphocyte Receptor 2
D: Variable Lymphocyte Receptor 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)150,28115
Polymers147,7164
Non-polymers2,56511
Water6,107339
1
A: Toll-like receptor 5b, Variable lymphocyte receptor B chimera
C: Variable Lymphocyte Receptor 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)75,2117
Polymers73,8582
Non-polymers1,3535
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Toll-like receptor 5b, Variable lymphocyte receptor B chimera
D: Variable Lymphocyte Receptor 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)75,0708
Polymers73,8582
Non-polymers1,2126
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)98.694, 112.102, 148.356
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number18
Space group name H-MP21212
Components on special symmetry positions
IDModelComponents
11A-1113-

HOH

21A-1141-

HOH

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Components

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Protein , 2 types, 4 molecules ABCD

#1: Protein Toll-like receptor 5b, Variable lymphocyte receptor B chimera


Mass: 51169.230 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Danio rerio (zebrafish), (gene. exp.) Eptatretus burgeri (inshore hagfish)
Gene: tlr5b, VLRB / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: F8W3J5, UniProt: Q4G1L2
#2: Protein Variable Lymphocyte Receptor 2


Mass: 22688.521 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Petromyzon marinus (sea lamprey) / Production host: Spodoptera frugiperda (fall armyworm)

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Sugars , 2 types, 8 molecules

#3: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 3 / Source method: isolated from a natural source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}}LINUCSPDB-CARE
#4: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 2 types, 342 molecules

#5: Chemical ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C2H6O2
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 339 / Source method: isolated from a natural source / Formula: H2O

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Details

Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.9 Å3/Da / Density % sol: 57.58 %
Crystal growTemperature: 293.15 K / Method: vapor diffusion, sitting drop
Details: 16% PEG 8K, 180 mM ammonium citrate, 90 mM MES pH 6.0, 10 mM urea

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 23-ID-D / Wavelength: 1.0332 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Oct 17, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.0332 Å / Relative weight: 1
ReflectionResolution: 2.298→50 Å / Num. obs: 72974 / % possible obs: 99.2 % / Redundancy: 5.7 % / CC1/2: 0.89 / Rmerge(I) obs: 0.09 / Rpim(I) all: 0.04 / Net I/σ(I): 20.2
Reflection shellResolution: 2.298→2.35 Å / Redundancy: 2.5 % / Mean I/σ(I) obs: 1.3 / Num. unique obs: 3213 / CC1/2: 0.45 / Rpim(I) all: 0.53 / % possible all: 88

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Processing

Software
NameVersionClassification
PHENIX1.9_1692refinement
HKL-2000data reduction
SCALEPACKdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3V47

3v47


Resolution: 2.298→49.452 Å / SU ML: 0.26 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 22.47
RfactorNum. reflection% reflection
Rfree0.2178 3616 4.96 %
Rwork0.1854 --
obs0.187 72923 98.64 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Refinement stepCycle: LAST / Resolution: 2.298→49.452 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms9776 0 166 339 10281
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00610157
X-RAY DIFFRACTIONf_angle_d0.9313794
X-RAY DIFFRACTIONf_dihedral_angle_d12.7633633
X-RAY DIFFRACTIONf_chiral_restr0.0371644
X-RAY DIFFRACTIONf_plane_restr0.0051765
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.2982-2.32840.3267980.30331954X-RAY DIFFRACTION74
2.3284-2.36030.31921200.28522516X-RAY DIFFRACTION93
2.3603-2.3940.33411140.26592650X-RAY DIFFRACTION98
2.394-2.42980.30711390.24772629X-RAY DIFFRACTION99
2.4298-2.46770.2741440.22692699X-RAY DIFFRACTION100
2.4677-2.50820.25681390.22092658X-RAY DIFFRACTION100
2.5082-2.55140.29451450.22062674X-RAY DIFFRACTION100
2.5514-2.59780.26241540.21052649X-RAY DIFFRACTION100
2.5978-2.64780.27671160.21652686X-RAY DIFFRACTION100
2.6478-2.70180.24881400.21442690X-RAY DIFFRACTION100
2.7018-2.76060.24281450.20662667X-RAY DIFFRACTION100
2.7606-2.82480.26551280.2042684X-RAY DIFFRACTION100
2.8248-2.89540.21961360.20652690X-RAY DIFFRACTION100
2.8954-2.97370.26551420.20842690X-RAY DIFFRACTION100
2.9737-3.06120.27031290.22142701X-RAY DIFFRACTION100
3.0612-3.160.25981220.21552706X-RAY DIFFRACTION100
3.16-3.27290.27921410.21452680X-RAY DIFFRACTION100
3.2729-3.40390.23651400.19842728X-RAY DIFFRACTION100
3.4039-3.55880.19951530.19512685X-RAY DIFFRACTION100
3.5588-3.74630.18881630.16182674X-RAY DIFFRACTION100
3.7463-3.9810.18731570.15472709X-RAY DIFFRACTION100
3.981-4.28820.17231550.14372715X-RAY DIFFRACTION100
4.2882-4.71940.1621280.14092746X-RAY DIFFRACTION100
4.7194-5.40160.1741400.14712766X-RAY DIFFRACTION100
5.4016-6.80260.21271580.18462770X-RAY DIFFRACTION100
6.8026-49.46340.2091700.18342891X-RAY DIFFRACTION100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.3412-0.3448-0.45641.78330.1591.93940.03680.0659-0.2012-0.12170.0019-0.13660.34740.2070.00510.33570.0345-0.06130.2366-0.04530.348514.271816.584249.1584
21.4611-0.1837-0.31611.29811.17451.6832-0.08610.35620.04480.2807-0.13640.2303-0.3771-0.15450.00020.34230.0095-0.04080.30260.00790.32770.133639.366762.2215
32.10051.21470.91682.22080.70731.3989-0.0039-0.02430.16850.2227-0.0159-0.1451-0.09110.0959-00.36540.0319-0.04390.2305-0.00550.276514.95452.899873.8898
41.31570.47541.14971.73020.04411.09660.33150.6263-0.1344-0.115-0.17850.6232-0.0689-1.03550.16790.37240.2083-0.03631.0415-0.00680.7764-28.304941.462537.0818
50.6798-0.15160.01710.94810.02160.14290.06220.4062-0.1282-0.2076-0.05330.62980.177-1.053-0.03680.3296-0.1077-0.13230.8356-0.03970.6468-21.832727.530636.3357
61.0492-0.5933-0.48410.9110.35910.84280.04780.033-0.2756-0.351-0.25420.52760.5245-0.7082-0.01850.5627-0.1259-0.1670.5478-0.08620.5403-9.639317.656127.9821
70.4322-0.4577-0.08020.48550.13260.35560.04690.2456-0.3551-0.3142-0.02620.08580.5691-0.1907-0.00680.6007-0.0572-0.11430.3858-0.10320.38212.064914.145825.1127
80.5882-0.3712-0.44350.5127-0.12190.90930.34010.3887-0.121-0.5962-0.24560.15620.1246-0.1344-00.72080.0264-0.05880.5122-0.13860.45362.36818.935317.2926
90.7591-0.6516-0.48210.89190.13120.56340.10930.43770.1182-0.6573-0.084-0.31580.17810.5737-0.00040.85660.0932-0.02170.7906-0.11550.55899.388521.731612.2638
100.546-0.552-0.22670.6379-0.0080.79090.34070.7854-0.0472-0.7187-0.3509-0.0677-0.1187-0.1007-0.00181.03910.15590.04330.9095-0.09930.46597.680624.57392.4774
111.1776-0.605-0.10540.47080.13380.04980.37980.74850.537-0.9996-0.1035-0.1234-0.3480.00160.0061.49540.17360.06791.184-0.07360.58838.566930.3002-5.9683
120.8106-0.78120.13460.7851-0.08740.08270.55720.92980.222-0.4831-0.1426-0.2454-0.56840.09880.071.46680.2530.03691.3830.08130.534.565835.6699-10.6623
133.02280.775-0.05970.47510.440.7508-0.05450.71421.1971-0.8476-0.08850.0606-0.3969-0.0643-0.37811.75810.18750.22921.45990.38750.84846.763747.3802-14.9607
140.0218-0.02310.02290.0377-0.05240.08350.2287-0.55390.02040.606-0.6120.0388-0.64120.57590.00070.62010.0470.00510.7574-0.05290.651732.251120.697856.3743
150.79080.0777-0.44480.1757-0.18310.36490.3748-0.9451-0.25020.4855-0.281-0.22360.22470.781-0.03540.4755-0.02530.01850.8633-0.23580.715133.676526.479154.2774
160.22320.1174-0.21540.3388-0.10950.54640.1561-0.37690.6150.2719-0.253-0.1307-0.48760.6673-0.00030.5239-0.10330.05450.839-0.19660.81936.942332.811148.3501
170.22490.2867-0.11190.3661-0.15730.18470.243-0.43610.34120.2329-0.2703-0.2305-0.07250.3732-0.00020.4904-0.08170.02851.12990.02890.912140.725329.421443.548
180.986-0.8155-0.93070.69380.79230.90470.018-0.3926-0.08870.613-0.1329-0.8594-0.63221.162-0.0140.5761-0.28380.20970.8019-0.03170.995743.394939.645340.4565
190.17690.27230.07370.43950.18310.2491-0.2593-0.40380.53840.38140.2418-0.2302-0.19650.6798-0.00020.43130.01980.07870.8788-0.06590.852142.316930.026138.0366
200.0158-0.05-0.04670.16110.14960.13890.1332-0.16560.2750.30880.3125-0.2083-0.5780.61260.00230.5214-0.20750.17231.07210.06340.96745.506539.873333.7097
210.1113-0.12290.29591.0349-0.38720.78980.3349-0.49130.2247-0.74770.2076-0.104-0.03540.14680.02910.63580.03150.13920.89580.02710.821441.977234.455230.3701
220.00870.017-0.01940.0624-0.04960.04780.0153-0.32240.3031-0.1039-0.6344-0.70.19490.3628-0.00110.5788-0.09830.19041.2102-0.05750.907448.664739.021726.439
230.1461-0.07520.11650.2707-0.10120.10030.3142-0.3274-0.7554-0.8129-0.31170.56720.74210.42120.00020.83370.17720.02560.9667-0.20310.913343.041725.605526.546
240.0435-0.0109-0.01340.00880.00680.01740.0770.7940.097-0.67880.07250.60960.2796-0.26420.00140.9553-0.035-0.00791.05430.01061.035637.504236.17120.475
252.8203-1.28311.00931.1129-0.10430.5997-0.49130.0261.25710.7986-0.2096-0.11380.03870.5245-0.12691.037-0.02290.20721.19520.23351.093244.363447.546521.8222
260.05570.053-0.01320.06540.00040.0129-0.16961.19290.0278-1.13970.44820.42520.03380.8120.00031.1916-0.1085-0.28451.5458-0.05270.8825-23.835935.264118.9799
270.508-0.6581-0.20170.87050.26110.07990.72220.8581-0.0386-0.8456-0.71160.8190.1165-0.49230.00420.86720.2119-0.10771.18320.01580.6032-20.644540.55317.7487
280.18130.27280.1890.50690.26980.37740.02820.77760.7033-1.06410.20320.23290.0019-0.84390.00050.91880.1882-0.03871.09670.27230.8542-17.480549.140919.0332
290.35830.1827-0.31210.47680.00380.8669-0.18440.18280.2765-0.47120.09560.007-0.50050.12880.00010.8799-0.0042-0.15080.90960.18521.1399-18.883459.091826.9083
300.20010.00240.01960.23560.14730.09360.302-0.86440.7041-0.3319-0.3720.94610.1274-0.07050.0011.1179-0.06960.22180.92770.04111.1824-19.711366.571234.9199
310.08210.09520.02670.28470.21590.20160.3439-0.19380.08430.16840.1-0.1029-0.50740.21150.00061.2576-0.1924-0.13671.26550.02391.2683-9.01371.045635.8269
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 24 through 267 )
2X-RAY DIFFRACTION2chain 'A' and (resid 268 through 303 )
3X-RAY DIFFRACTION3chain 'A' and (resid 304 through 466 )
4X-RAY DIFFRACTION4chain 'B' and (resid 24 through 63 )
5X-RAY DIFFRACTION5chain 'B' and (resid 64 through 152 )
6X-RAY DIFFRACTION6chain 'B' and (resid 153 through 203 )
7X-RAY DIFFRACTION7chain 'B' and (resid 204 through 232 )
8X-RAY DIFFRACTION8chain 'B' and (resid 233 through 267 )
9X-RAY DIFFRACTION9chain 'B' and (resid 268 through 303 )
10X-RAY DIFFRACTION10chain 'B' and (resid 304 through 356 )
11X-RAY DIFFRACTION11chain 'B' and (resid 357 through 385 )
12X-RAY DIFFRACTION12chain 'B' and (resid 386 through 418 )
13X-RAY DIFFRACTION13chain 'B' and (resid 419 through 464 )
14X-RAY DIFFRACTION14chain 'C' and (resid 23 through 36 )
15X-RAY DIFFRACTION15chain 'C' and (resid 37 through 57 )
16X-RAY DIFFRACTION16chain 'C' and (resid 58 through 96 )
17X-RAY DIFFRACTION17chain 'C' and (resid 97 through 110 )
18X-RAY DIFFRACTION18chain 'C' and (resid 111 through 120 )
19X-RAY DIFFRACTION19chain 'C' and (resid 121 through 134 )
20X-RAY DIFFRACTION20chain 'C' and (resid 135 through 144 )
21X-RAY DIFFRACTION21chain 'C' and (resid 145 through 163 )
22X-RAY DIFFRACTION22chain 'C' and (resid 164 through 175 )
23X-RAY DIFFRACTION23chain 'C' and (resid 176 through 186 )
24X-RAY DIFFRACTION24chain 'C' and (resid 187 through 199 )
25X-RAY DIFFRACTION25chain 'C' and (resid 200 through 212 )
26X-RAY DIFFRACTION26chain 'D' and (resid 23 through 36 )
27X-RAY DIFFRACTION27chain 'D' and (resid 37 through 57 )
28X-RAY DIFFRACTION28chain 'D' and (resid 58 through 96 )
29X-RAY DIFFRACTION29chain 'D' and (resid 97 through 163 )
30X-RAY DIFFRACTION30chain 'D' and (resid 164 through 186 )
31X-RAY DIFFRACTION31chain 'D' and (resid 187 through 212 )

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