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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 6be6 | ||||||||||||
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タイトル | ADAM10 Extracellular Domain | ||||||||||||
![]() | Disintegrin and metalloproteinase domain-containing protein 10 | ||||||||||||
![]() | MEMBRANE PROTEIN / ADAM10 | ||||||||||||
機能・相同性 | ![]() ADAM10 endopeptidase / constitutive protein ectodomain proteolysis / regulation of vasculature development / epidermal growth factor receptor ligand maturation / monocyte activation / metalloendopeptidase activity involved in amyloid precursor protein catabolic process / postsynapse organization / Constitutive Signaling by NOTCH1 t(7;9)(NOTCH1:M1580_K2555) Translocation Mutant / protein catabolic process at postsynapse / pore complex assembly ...ADAM10 endopeptidase / constitutive protein ectodomain proteolysis / regulation of vasculature development / epidermal growth factor receptor ligand maturation / monocyte activation / metalloendopeptidase activity involved in amyloid precursor protein catabolic process / postsynapse organization / Constitutive Signaling by NOTCH1 t(7;9)(NOTCH1:M1580_K2555) Translocation Mutant / protein catabolic process at postsynapse / pore complex assembly / perinuclear endoplasmic reticulum / positive regulation of T cell chemotaxis / tetraspanin-enriched microdomain / regulation of Notch signaling pathway / NOTCH4 Activation and Transmission of Signal to the Nucleus / metallodipeptidase activity / negative regulation of cell adhesion / adherens junction organization / regulation of postsynapse organization / clathrin-coated vesicle / Golgi-associated vesicle / regulation of neurotransmitter receptor localization to postsynaptic specialization membrane / cochlea development / Signaling by EGFR / pore complex / amyloid precursor protein catabolic process / Collagen degradation / tertiary granule membrane / membrane protein ectodomain proteolysis / response to tumor necrosis factor / EPH-ephrin mediated repulsion of cells / extracellular matrix disassembly / specific granule membrane / Notch signaling pathway / Degradation of the extracellular matrix / Constitutive Signaling by NOTCH1 HD Domain Mutants / NOTCH2 Activation and Transmission of Signal to the Nucleus / Activated NOTCH1 Transmits Signal to the Nucleus / integrin-mediated signaling pathway / adherens junction / NOTCH3 Activation and Transmission of Signal to the Nucleus / Post-translational protein phosphorylation / synaptic membrane / protein processing / metalloendopeptidase activity / SH3 domain binding / Constitutive Signaling by NOTCH1 PEST Domain Mutants / Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / metallopeptidase activity / integrin binding / cell-cell signaling / positive regulation of cell growth / endopeptidase activity / in utero embryonic development / postsynaptic density / positive regulation of cell migration / protein phosphorylation / Amyloid fiber formation / endoplasmic reticulum lumen / axon / Golgi membrane / signaling receptor binding / negative regulation of gene expression / focal adhesion / intracellular membrane-bounded organelle / positive regulation of cell population proliferation / dendrite / Neutrophil degranulation / protein kinase binding / glutamatergic synapse / cell surface / Golgi apparatus / protein homodimerization activity / extracellular exosome / metal ion binding / nucleus / membrane / plasma membrane / cytoplasm 類似検索 - 分子機能 | ||||||||||||
生物種 | ![]() | ||||||||||||
手法 | ![]() ![]() ![]() | ||||||||||||
![]() | Seegar, T.C.M. | ||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structural Basis for Regulated Proteolysis by the alpha-Secretase ADAM10. 著者: Seegar, T.C.M. / Killingsworth, L.B. / Saha, N. / Meyer, P.A. / Patra, D. / Zimmerman, B. / Janes, P.W. / Rubinstein, E. / Nikolov, D.B. / Skiniotis, G. / Kruse, A.C. / Blacklow, S.C. | ||||||||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 355.9 KB | 表示 | ![]() |
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PDB形式 | ![]() | 289.9 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 1.7 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 1.7 MB | 表示 | |
XML形式データ | ![]() | 75.7 KB | 表示 | |
CIF形式データ | ![]() | 98.4 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
-タンパク質 , 1種, 4分子 ABCD
#1: タンパク質 | 分子量: 49562.371 Da / 分子数: 4 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() 発現宿主: ![]() ![]() 参照: UniProt: O14672, ADAM10 endopeptidase |
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-糖 , 4種, 4分子
#2: 多糖 | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-4)-2-acetamido-2- ...alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose |
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#3: 多糖 | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose |
#4: 多糖 | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-4)-2-acetamido-2- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose |
#5: 多糖 | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose |
-非ポリマー , 4種, 398分子 






#6: 化合物 | ChemComp-ZN / #7: 化合物 | ChemComp-CA / #8: 化合物 | ChemComp-SO4 / #9: 水 | ChemComp-HOH / | |
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-詳細
Has protein modification | Y |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.65 Å3/Da / 溶媒含有率: 53.56 % |
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結晶化 | 温度: 295 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 7.5 詳細: 0.26M Ammonium Sulfate 100mM HEPES pH 7.5 21.75 % PEG8000 |
-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: DECTRIS PILATUS 6M-F / 検出器: PIXEL / 日付: 2016年10月20日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.979 Å / 相対比: 1 |
反射 | 解像度: 2.8→47.476 Å / Num. obs: 52456 / % possible obs: 97.37 % / 冗長度: 3.1 % / CC1/2: 0.959 / Net I/av σ(I): 4.8 / Net I/σ(I): 4.8 |
反射 シェル | 解像度: 2.8→2.9 Å / 冗長度: 3.1 % / Mean I/σ(I) obs: 1.35 / Num. unique obs: 5184 / CC1/2: 0.323 / % possible all: 97.99 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: 1BKC 解像度: 2.8→47.476 Å / SU ML: 0.54 / 交差検証法: FREE R-VALUE / σ(F): 1.34 / 位相誤差: 30.59 / 立体化学のターゲット値: ML
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溶媒の処理 | 減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 2.8→47.476 Å
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拘束条件 |
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LS精密化 シェル |
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