Entry | Database: PDB / ID: 6bcm |
---|
Title | Structure of a Self-inhibited N475A variant of the Venezuelan Equine Encephalitis Virus (VEEV) nsP2 cysteine protease |
---|
Components | Protease nsP2 |
---|
Keywords | VIRAL PROTEIN / alphavirus / nsP2 / nonstructural protein / cysteine protease / self-inhibited / N475A / S-adenosyl-L-methionine methyltransferase |
---|
Function / homology | Function and homology information
polynucleotide 5'-phosphatase / ADP-ribosyl-[dinitrogen reductase] hydrolase activity / : / O-acetyl-ADP-ribose deacetylase activity / host cell filopodium / ADP-ribose 1''-phosphate phosphatase / mRNA methyltransferase activity / mRNA 5'-triphosphate monophosphatase activity / mRNA 5'-phosphatase / polynucleotide 5'-phosphatase activity ...polynucleotide 5'-phosphatase / ADP-ribosyl-[dinitrogen reductase] hydrolase activity / : / O-acetyl-ADP-ribose deacetylase activity / host cell filopodium / ADP-ribose 1''-phosphate phosphatase / mRNA methyltransferase activity / mRNA 5'-triphosphate monophosphatase activity / mRNA 5'-phosphatase / polynucleotide 5'-phosphatase activity / polynucleotide adenylyltransferase / poly(A) RNA polymerase activity / symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity / 7-methylguanosine mRNA capping / cysteine-type peptidase activity / ribonucleoside triphosphate phosphatase activity / Transferases; Transferring one-carbon groups; Methyltransferases / host cell cytoplasmic vesicle membrane / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / nucleoside-triphosphate phosphatase / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / RNA helicase activity / RNA helicase / RNA-directed RNA polymerase / viral RNA genome replication / RNA-dependent RNA polymerase activity / DNA-templated transcription / host cell nucleus / GTP binding / host cell plasma membrane / ATP hydrolysis activity / proteolysis / RNA binding / ATP binding / membrane / metal ion bindingSimilarity search - Function Alphavirus nsP2 protease domain / : / : / : / Non-structural protein 3, zinc-binding domain / Tomato mosaic virus helicase, N-terminal domain / Alphavirus nsP2 protease domain superfamily / Alphavirus nsp2 protease (nsp2pro) domain / Peptidase family C9 / Alphavirus nsp2 protease (nsp2pro) domain profile. ...Alphavirus nsP2 protease domain / : / : / : / Non-structural protein 3, zinc-binding domain / Tomato mosaic virus helicase, N-terminal domain / Alphavirus nsP2 protease domain superfamily / Alphavirus nsp2 protease (nsp2pro) domain / Peptidase family C9 / Alphavirus nsp2 protease (nsp2pro) domain profile. / Viral methyltransferase / Alphavirus-like methyltransferase (MT) domain / Alphavirus-like methyltransferase (MT) domain profile. / Tymovirus, RNA-dependent RNA polymerase / RNA dependent RNA polymerase / Viral (Superfamily 1) RNA helicase / Cathepsin B; Chain A / Vaccinia Virus protein VP39 / (+) RNA virus helicase core domain / (+)RNA virus helicase core domain profile. / Non-structural protein 3, X-domain-like / Macro domain / Appr-1"-p processing enzyme / Macro domain / Macro domain profile. / Macro domain-like / RNA-directed RNA polymerase, catalytic domain / RdRp of positive ssRNA viruses catalytic domain profile. / S-adenosyl-L-methionine-dependent methyltransferase superfamily / DNA/RNA polymerase superfamily / Alpha-Beta Complex / Rossmann fold / P-loop containing nucleoside triphosphate hydrolase / 3-Layer(aba) Sandwich / Alpha BetaSimilarity search - Domain/homology |
---|
Biological species | Venezuelan equine encephalitis virus |
---|
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.1 Å |
---|
Authors | Compton, J.R. / Legler, P.M. |
---|
Funding support | United States, 2items Organization | Grant number | Country |
---|
Defense Threat Reduction Agency (DTRA) | CBCall4-CBM-05-2-0019 | United States | Defense Threat Reduction Agency (DTRA) | CB-SEED-SEED09-2-0061 | United States |
|
---|
Citation | Journal: Biochemistry / Year: 2017 Title: Mutation of Asn-475 in the Venezuelan Equine Encephalitis Virus nsP2 Cysteine Protease Leads to a Self-Inhibited State. Authors: Compton, J.R. / Mickey, M.J. / Hu, X. / Marugan, J.J. / Legler, P.M. |
---|
History | Deposition | Oct 20, 2017 | Deposition site: RCSB / Processing site: RCSB |
---|
Revision 1.0 | Nov 8, 2017 | Provider: repository / Type: Initial release |
---|
Revision 1.1 | Dec 13, 2017 | Group: Database references / Category: citation / citation_author Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.title / _citation_author.name |
---|
Revision 1.2 | Jan 17, 2018 | Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization |
---|
Revision 1.3 | Apr 18, 2018 | Group: Data collection / Category: diffrn_detector / Item: _diffrn_detector.detector |
---|
Revision 1.4 | Jan 1, 2020 | Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization |
---|
Revision 1.5 | Oct 4, 2023 | Group: Data collection / Database references / Refinement description Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession |
---|
|
---|