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- PDB-6anr: Crystal structure of a self resistance protein ClbS from colibact... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6anr | ||||||
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Title | Crystal structure of a self resistance protein ClbS from colibactin biosynthetic gene cluster | ||||||
![]() | Colibactin self-protection protein ClbS | ||||||
![]() | HYDROLASE / Self resistance protein | ||||||
Function / homology | Protein of unknown function DUF1706 / Protein of unknown function (DUF1706) / dinb family like domain / DinB/YfiT-like putative metalloenzymes / Four Helix Bundle (Hemerythrin (Met), subunit A) / Up-down Bundle / Mainly Alpha / Colibactin self-protection protein ClbS![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Tripathi, P. / Bruner, S.D. | ||||||
![]() | ![]() Title: ClbS Is a Cyclopropane Hydrolase That Confers Colibactin Resistance. Authors: Tripathi, P. / Shine, E.E. / Healy, A.R. / Kim, C.S. / Herzon, S.B. / Bruner, S.D. / Crawford, J.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 51.9 KB | Display | ![]() |
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PDB format | ![]() | 35.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 419.7 KB | Display | ![]() |
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Full document | ![]() | 421 KB | Display | |
Data in XML | ![]() | 9.6 KB | Display | |
Data in CIF | ![]() | 13.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4n6cS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 20747.664 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 39.24 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 0.2 M Ammonium acetate, 0.1 M Sodium acetate trihydrate pH 4.6, 30% w/v Polyethylene glycol 4,000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Nov 16, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9787 Å / Relative weight: 1 |
Reflection | Resolution: 2.098→38.37 Å / Num. obs: 10332 / % possible obs: 99.89 % / Redundancy: 14.2 % / Rmerge(I) obs: 0.0968 / Net I/σ(I): 25.37 |
Reflection shell | Resolution: 2.098→2.173 Å / Redundancy: 14 % / Rmerge(I) obs: 0.4939 / Num. unique obs: 1014 / % possible all: 98.93 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4N6C Resolution: 2.098→38.37 Å / SU ML: 0.22 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 21.53
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.098→38.37 Å
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Refine LS restraints |
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LS refinement shell |
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