- PDB-6a79: Crystal structure of the fifth immunoglobulin domain (Ig5) of hum... -
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基本情報
登録情報
データベース: PDB / ID: 6a79
タイトル
Crystal structure of the fifth immunoglobulin domain (Ig5) of human Robo1 in complex with the mutant scFv fragment (P103A) of murine monoclonal antibody B5209B
要素
Heavy chain of the anti-human Robo1 antibody B5209B scFv
Light chain region of the anti-human Robo1 antibody B5209B scFv
Roundabout homolog 1
キーワード
IMMUNE SYSTEM / hepatocellular carcinoma antigen / angiogenesis / antibody drug / single-chain variable fragment
機能・相同性
機能・相同性情報
chemorepulsion involved in postnatal olfactory bulb interneuron migration / negative regulation of negative chemotaxis / Regulation of cortical dendrite branching / negative regulation of mammary gland epithelial cell proliferation / LRR domain binding / negative regulation of chemokine-mediated signaling pathway / heart induction / axon guidance receptor activity / Netrin-1 signaling / positive regulation of vascular endothelial growth factor signaling pathway ...chemorepulsion involved in postnatal olfactory bulb interneuron migration / negative regulation of negative chemotaxis / Regulation of cortical dendrite branching / negative regulation of mammary gland epithelial cell proliferation / LRR domain binding / negative regulation of chemokine-mediated signaling pathway / heart induction / axon guidance receptor activity / Netrin-1 signaling / positive regulation of vascular endothelial growth factor signaling pathway / Role of ABL in ROBO-SLIT signaling / Regulation of commissural axon pathfinding by SLIT and ROBO / SLIT2:ROBO1 increases RHOA activity / Roundabout signaling pathway / Inactivation of CDC42 and RAC1 / endocardial cushion formation / Signaling by ROBO receptors / pulmonary valve morphogenesis / outflow tract septum morphogenesis / positive regulation of vascular endothelial growth factor receptor signaling pathway / cell migration involved in sprouting angiogenesis / aortic valve morphogenesis / Activation of RAC1 / axon midline choice point recognition / aorta development / positive regulation of Rho protein signal transduction / positive regulation of axonogenesis / ventricular septum morphogenesis / positive regulation of Notch signaling pathway / homophilic cell adhesion via plasma membrane adhesion molecules / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of MAP kinase activity / negative regulation of cell migration / activation of cysteine-type endopeptidase activity involved in apoptotic process / Regulation of expression of SLITs and ROBOs / nervous system development / cell adhesion / neuron projection / axon / negative regulation of gene expression / positive regulation of gene expression / cell surface / identical protein binding / plasma membrane / cytoplasm 類似検索 - 分子機能
A: Roundabout homolog 1 L: Light chain region of the anti-human Robo1 antibody B5209B scFv H: Heavy chain of the anti-human Robo1 antibody B5209B scFv B: Roundabout homolog 1 M: Light chain region of the anti-human Robo1 antibody B5209B scFv I: Heavy chain of the anti-human Robo1 antibody B5209B scFv ヘテロ分子
A: Roundabout homolog 1 L: Light chain region of the anti-human Robo1 antibody B5209B scFv H: Heavy chain of the anti-human Robo1 antibody B5209B scFv ヘテロ分子
B: Roundabout homolog 1 M: Light chain region of the anti-human Robo1 antibody B5209B scFv I: Heavy chain of the anti-human Robo1 antibody B5209B scFv ヘテロ分子
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.9 Å / 相対比: 1
反射
解像度: 2.3→50 Å / Num. obs: 30034 / % possible obs: 95.1 % / 冗長度: 5.6 % / Net I/σ(I): 7.4
反射 シェル
解像度: 2.3→2.38 Å
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解析
ソフトウェア
名称
バージョン
分類
REFMAC
5.8.0158
精密化
HKL-2000
データ削減
Coot
モデル構築
PHASER
位相決定
精密化
構造決定の手法: 分子置換 / 解像度: 2.31→50 Å / Cor.coef. Fo:Fc: 0.885 / Cor.coef. Fo:Fc free: 0.871 / SU B: 14.124 / SU ML: 0.291 / 交差検証法: THROUGHOUT / ESU R: 0.545 / ESU R Free: 0.303 / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS