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- PDB-5zf3: Crystal Structures of Endo-beta-1,4-xylanase II Complexed with Xy... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5zf3 | |||||||||
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Title | Crystal Structures of Endo-beta-1,4-xylanase II Complexed with Xylotriose | |||||||||
![]() | Endo-1,4-beta-xylanase 2 | |||||||||
![]() | HYDROLASE / xylanase II / complex / Co-crystallization / Xylotriose | |||||||||
Function / homology | ![]() endo-1,4-beta-xylanase activity / endo-1,4-beta-xylanase / xylan catabolic process / extracellular region Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Zhang, X. / Wan, Q. / Li, Z. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal Structures of Endo-beta-1,4-xylanase II Complexed with Xylotriose Authors: Zhang, X. / Wan, Q. / Li, Z. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 130.5 KB | Display | ![]() |
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PDB format | ![]() | 101.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6jxlC ![]() 2dfcS S: Starting model for refinement C: citing same article ( |
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Similar structure data | |
Experimental dataset #1 | Data reference: ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 20727.338 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 56765 / BCRC 32924 / NRRL 11460 / Rut C-30 / Gene: xyn2, M419DRAFT_124931 / Production host: ![]() ![]() | ||
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#2: Polysaccharide | beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose / 4beta-beta-xylotriose | ||
#3: Chemical | ChemComp-GOL / | ||
#4: Chemical | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.37 Å3/Da / Density % sol: 48.02 % |
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Crystal grow | Temperature: 291 K / Method: evaporation / pH: 6 / Details: 20% PEG 8000, 0.2M NaI, 0.1M MES |
-Data collection
Diffraction | Mean temperature: 80 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jan 20, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.2→39.684 Å / Num. obs: 61740 / % possible obs: 99.09 % / Redundancy: 6.1 % / Biso Wilson estimate: 11.1 Å2 / Net I/σ(I): 22.92 |
Reflection shell | Resolution: 1.2→1.243 Å / Redundancy: 6 % / Mean I/σ(I) obs: 3.87 / Num. unique obs: 5884 / % possible all: 94.84 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: 2DFC Resolution: 1.2→39.684 Å / SU ML: 0.1 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 13.44 Details: The structure factor file contains friedel pairs in F_PLUS/MINUS and I_PLUS/MINUS columns.
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 66.43 Å2 / Biso mean: 15.2352 Å2 / Biso min: 6.09 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.2→39.684 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 22
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