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- PDB-5z9a: Crystal structure of chorismate synthase from Pseudomonas aeruginosa -
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Open data
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Basic information
Entry | Database: PDB / ID: 5z9a | ||||||
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Title | Crystal structure of chorismate synthase from Pseudomonas aeruginosa | ||||||
![]() | Chorismate synthase | ||||||
![]() | LYASE | ||||||
Function / homology | ![]() chorismate synthase / chorismate synthase activity / chorismate biosynthetic process / aromatic amino acid family biosynthetic process Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Iqbal, N. / Chaudhary, A. / Kaur, P. / Sharma, S. / Singh, T.P. | ||||||
![]() | ![]() Title: Crystal structure of chorismate synthase from Pseudomonas aeruginosa Authors: Iqbal, N. / Chaudhary, A. / Kaur, P. / Sharma, S. / Singh, T.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 198.4 KB | Display | ![]() |
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PDB format | ![]() | 159.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 442.3 KB | Display | ![]() |
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Full document | ![]() | 454.5 KB | Display | |
Data in XML | ![]() | 19.2 KB | Display | |
Data in CIF | ![]() | 26 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1r53S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 1 / Ens-ID: 1 / Beg auth comp-ID: MET / Beg label comp-ID: MET / End auth comp-ID: GLN / End label comp-ID: GLN / Refine code: 1 / Auth seq-ID: 1 - 349 / Label seq-ID: 1 - 349
NCS oper:
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Components
#1: Protein | Mass: 39036.066 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: LESB58 / Gene: aroC, PLES_36461 / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.11 Å3/Da / Density % sol: 41.62 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion / pH: 7.5 / Details: 10mM HEPES pH 7.5, 8% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Sep 3, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9724 Å / Relative weight: 1 |
Reflection | Resolution: 2.79→66.28 Å / Num. obs: 17307 / % possible obs: 96.36 % / Redundancy: 5.92 % / Rmerge(I) obs: 0.132 / Net I/σ(I): 11.05 |
Reflection shell | Resolution: 2.791→2.891 Å / Rmerge(I) obs: 0.43 / Num. measured obs: 7302 / Num. unique obs: 887 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1R53 Resolution: 2.79→66.28 Å / Cor.coef. Fo:Fc: 0.955 / Cor.coef. Fo:Fc free: 0.927 / SU B: 34.162 / SU ML: 0.297 / Cross valid method: THROUGHOUT / ESU R: 0.898 / ESU R Free: 0.377 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 96.169 Å2
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Refinement step | Cycle: 1 / Resolution: 2.79→66.28 Å
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Refine LS restraints |
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