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Yorodumi- PDB-1d4c: CRYSTAL STRUCTURE OF THE UNCOMPLEXED FORM OF THE FLAVOCYTOCHROME ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1d4c | |||||||||
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Title | CRYSTAL STRUCTURE OF THE UNCOMPLEXED FORM OF THE FLAVOCYTOCHROME C FUMARATE REDUCTASE OF SHEWANELLA PUTREFACIENS STRAIN MR-1 | |||||||||
Components | FLAVOCYTOCHROME C FUMARATE REDUCTASE | |||||||||
Keywords | OXIDOREDUCTASE / TETRAHEME FLAVOCYTOCHROME C | |||||||||
Function / homology | Function and homology information fumarate reductase (cytochrome) / steroid metabolic process / FMN binding / periplasmic space / oxidoreductase activity / metal ion binding Similarity search - Function | |||||||||
Biological species | Shewanella oneidensis (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.9 Å | |||||||||
Authors | Leys, D. / Tsapin, A.S. / Meyer, T.E. / Cusanovich, M.A. / Van Beeumen, J.J. | |||||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1999 Title: Structure and mechanism of the flavocytochrome c fumarate reductase of Shewanella putrefaciens MR-1. Authors: Leys, D. / Tsapin, A.S. / Nealson, K.H. / Meyer, T.E. / Cusanovich, M.A. / Van Beeumen, J.J. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1d4c.cif.gz | 440 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1d4c.ent.gz | 369.3 KB | Display | PDB format |
PDBx/mmJSON format | 1d4c.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1d4c_validation.pdf.gz | 1.7 MB | Display | wwPDB validaton report |
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Full document | 1d4c_full_validation.pdf.gz | 1.9 MB | Display | |
Data in XML | 1d4c_validation.xml.gz | 73.4 KB | Display | |
Data in CIF | 1d4c_validation.cif.gz | 99.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/d4/1d4c ftp://data.pdbj.org/pub/pdb/validation_reports/d4/1d4c | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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4 |
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Unit cell |
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-Components
#1: Protein | Mass: 59974.734 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Shewanella oneidensis (bacteria) / Strain: MR1 / References: UniProt: P83223, succinate dehydrogenase #2: Chemical | ChemComp-HEC / #3: Chemical | ChemComp-FAD / #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.77 Å3/Da / Density % sol: 55.6 % | ||||||||||||||||||||
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: ammonium sulfate with added PEG 400, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 21K | ||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: SYNCHROTRON / Site: LURE / Beamline: D41A |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.9→20 Å / Num. all: 266443 / Num. obs: 59920 / % possible obs: 93.7 % / Rmerge(I) obs: 0.083 |
Reflection | *PLUS Num. measured all: 266443 |
-Processing
Software |
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Refinement | Resolution: 2.9→15 Å
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Refinement step | Cycle: LAST / Resolution: 2.9→15 Å
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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