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- PDB-5z73: Crystal structure of alkaline/neutral invertase InvB from Anabaen... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5z73 | ||||||
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Title | Crystal structure of alkaline/neutral invertase InvB from Anabaena sp. PCC 7120 | ||||||
![]() | Alr0819 protein | ||||||
![]() | HYDROLASE / alkaline/neutral invertases / cyanobacteria / glycoside hydrolase family 100 / sucrose hydrolysis | ||||||
Function / homology | Glycosyl hydrolase family 100 / Alkaline and neutral invertase / endo-alpha-N-acetylgalactosaminidase activity / beta-fructofuranosidase activity / beta-fructofuranosidase / Six-hairpin glycosidase-like superfamily / Six-hairpin glycosidase superfamily / carbohydrate metabolic process / beta-fructofuranosidase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Xie, J. / Hu, H.X. / Cai, K. / Yang, F. / Jiang, Y.L. / Chen, Y. / Zhou, C.Z. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural and enzymatic analyses of Anabaena heterocyst-specific alkaline invertase InvB. Authors: Xie, J. / Hu, H.X. / Cai, K. / Xia, L.Y. / Yang, F. / Jiang, Y.L. / Chen, Y. / Zhou, C.Z. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 182.7 KB | Display | ![]() |
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PDB format | ![]() | 152.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 446.7 KB | Display | ![]() |
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Full document | ![]() | 453 KB | Display | |
Data in XML | ![]() | 32.3 KB | Display | |
Data in CIF | ![]() | 45.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 52609.516 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: PCC 7120 / SAG 25.82 / UTEX 2576 / Gene: invB, alr0819 / Production host: ![]() ![]() #2: Chemical | ChemComp-GOL / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.71 Å3/Da / Density % sol: 54.6 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 6 Details: 0.7M lithium chloride, 6% polyethylene glycol 6000, 0.1M MES, pH 6.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Mar 27, 2016 |
Radiation | Monochromator: SAGITALLY FOCUSED Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97916 Å / Relative weight: 1 |
Reflection | Resolution: 1.93→50 Å / Num. obs: 82269 / % possible obs: 97.6 % / Redundancy: 2.7 % / Rmerge(I) obs: 0.078 / Net I/σ(I): 15.1 |
Reflection shell | Resolution: 1.93→2 Å / Redundancy: 2.7 % / Rmerge(I) obs: 0.505 / Mean I/σ(I) obs: 2.3 / Num. unique obs: 8274 / % possible all: 98.9 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 41.334 Å2
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Refinement step | Cycle: 1 / Resolution: 1.93→31.41 Å
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Refine LS restraints |
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