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- PDB-5ye1: structure of endo-lysosomal TRPML1 channel inserting into amphipo... -

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Database: PDB / ID: 5ye1
Titlestructure of endo-lysosomal TRPML1 channel inserting into amphipol: state 2
KeywordsMEMBRANE PROTEIN / mTRPML1 / mucolipidosis type IV / structual comparisons / combined regulation mechanism
Specimen sourceMus musculus / mammal / House mouse /
MethodElectron microscopy (5.8 Å resolution / Particle / Single particle) / Transmission electron microscopy
AuthorsYang, M. / Gao, N.
CitationProtein Cell, 2017, 8, 834-847

Protein Cell, 2017, 8, 834-847 Yorodumi Papers
Cryo-EM structures of the mammalian endo-lysosomal TRPML1 channel elucidate the combined regulation mechanism.
Sensen Zhang / Ningning Li / Wenwen Zeng / Ning Gao / Maojun Yang

Validation Report
SummaryFull reportAbout validation report
DateDeposition: Sep 15, 2017 / Release: Dec 27, 2017

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Deposited unit
A: Mucolipin-1
B: Mucolipin-1
C: Mucolipin-1
D: Mucolipin-1

Theoretical massNumber of molelcules
Total (without water)262,2944

TypeNameSymmetry operationNumber
identity operation1_5551


#1: Protein/peptide
Mucolipin-1 / Mucolipidin / Transient receptor potential-mucolipin 1 / TRPML1

Mass: 65573.617 Da / Num. of mol.: 4 / Source: (gene. exp.) Mus musculus / mammal / House mouse / / Gene: Mcoln1 / Production host: Homo sapiens / References: UniProt: Q99J21

Experimental details


EM experimentAggregation state: PARTICLE / Reconstruction method: SINGLE PARTICLE

Sample preparation

ComponentName: mammalian endo-lysosomal TRPML1 channel inserting into amphipol
Source (natural)Organism: Mus musculus
Source (recombinant)Organism: Homo sapiens
Buffer solutionpH: 7.4
SpecimenEmbedding applied: YES / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
EM embeddingMaterial: Amorphous ice
VitrificationCryogen name: ETHANE

Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyMicroscope model: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD
Image recordingElectron dose: 50 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k)


EM softwareName: RELION / Version: 2 / Category: RECONSTRUCTION
3D reconstructionResolution: 5.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 167000 / Symmetry type: POINT

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