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- PDB-5xu2: Crystal Structure of Transketolase in complex with TPP_III and fr... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5xu2 | ||||||
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Title | Crystal Structure of Transketolase in complex with TPP_III and fructose-6-phosphate from Pichia Stipitis | ||||||
![]() | Transketolase | ||||||
![]() | TRANSFERASE / transketolase | ||||||
Function / homology | ![]() transketolase / transketolase activity / pentose-phosphate shunt / nucleus / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Li, T.L. / Hsu, N.S. / Wang, Y.L. | ||||||
![]() | ![]() Title: Evidence of Diradicals Involved in the Yeast Transketolase Catalyzed Keto-Transferring Reactions. Authors: Hsu, N.S. / Wang, Y.L. / Lin, K.H. / Chang, C.F. / Ke, S.C. / Lyu, S.Y. / Hsu, L.J. / Li, Y.S. / Chen, S.C. / Wang, K.C. / Li, T.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 307.2 KB | Display | ![]() |
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PDB format | ![]() | 240.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.7 MB | Display | ![]() |
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Full document | ![]() | 2.7 MB | Display | |
Data in XML | ![]() | 33.6 KB | Display | |
Data in CIF | ![]() | 53.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5xpsC ![]() 5xqaC ![]() 5xqkC ![]() 5xrvC ![]() 5xt0C ![]() 5xt4C ![]() 5xtxC ![]() 5xu9C ![]() 5hyvS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 75102.453 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: CBS 6054 / Gene: TKT, TKT1, PICST_67105 / Production host: ![]() ![]() |
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-Non-polymers , 6 types, 792 molecules ![](data/chem/img/CA.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/F6R.gif)
![](data/chem/img/8EL.gif)
![](data/chem/img/TPP.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/F6R.gif)
![](data/chem/img/8EL.gif)
![](data/chem/img/TPP.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-CA / | ||||||||
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#3: Chemical | #4: Chemical | ChemComp-F6R / | #5: Chemical | ChemComp-8EL / | #6: Chemical | ChemComp-TPP / | #7: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.09 Å3/Da / Density % sol: 60.19 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: 0.1M MES, 0.1M NaCl, PEG 400 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX300HE / Detector: CCD / Date: Apr 14, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 0.97→30 Å / Num. obs: 542392 / % possible obs: 99 % / Redundancy: 10.7 % / Rmerge(I) obs: 0.1 / Net I/σ(I): 19.9 |
Reflection shell | Resolution: 0.97→1 Å / Rmerge(I) obs: 0.66 / Num. unique obs: 53961 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5HYV Resolution: 0.97→30 Å / Cor.coef. Fo:Fc: 0.977 / Cor.coef. Fo:Fc free: 0.974 / SU B: 0.401 / SU ML: 0.01 / Cross valid method: THROUGHOUT / ESU R: 0.015 / ESU R Free: 0.016 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 10.945 Å2
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Refinement step | Cycle: 1 / Resolution: 0.97→30 Å
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Refine LS restraints |
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