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Yorodumi- PDB-5xql: Crystal structure of a Pseudomonas aeruginosa transcriptional reg... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5xql | ||||||
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Title | Crystal structure of a Pseudomonas aeruginosa transcriptional regulator | ||||||
Components | Multidrug-efflux transporter 1 regulator | ||||||
Keywords | TRANSCRIPTION / Pseudomonas aeruginosa / transcriptional regulator | ||||||
Function / homology | Function and homology information DNA-binding transcription activator activity / protein-DNA complex / transcription cis-regulatory region binding / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / positive regulation of DNA-templated transcription / DNA binding Similarity search - Function | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.494 Å | ||||||
Authors | Raju, H. / Sharma, R. | ||||||
Citation | Journal: Biochem. Biophys. Res. Commun. / Year: 2017 Title: Crystal structure of BrlR with c-di-GMP Authors: Raju, H. / Sharma, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5xql.cif.gz | 126 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5xql.ent.gz | 98.3 KB | Display | PDB format |
PDBx/mmJSON format | 5xql.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xq/5xql ftp://data.pdbj.org/pub/pdb/validation_reports/xq/5xql | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 33439.750 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) Gene: bmrR, AO964_25340, AOY09_00728, B0B20_02615, PAERUG_E15_London_28_01_14_00871, PAERUG_P32_London_17_VIM_2_10_11_01154 Production host: Escherichia coli (E. coli) / References: UniProt: A0A069Q416, UniProt: Q9HUT5*PLUS |
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#2: Chemical |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.29 Å3/Da / Density % sol: 62.57 % |
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Crystal grow | Temperature: 293.15 K / Method: evaporation / pH: 6.5 / Details: 0.15 M Mg Cl2, 0.1 M Bis-Tris pH 6.5, 25% PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: BM14 / Wavelength: 0.98 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Nov 16, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 2.49→35.07 Å / Num. obs: 12920 / % possible obs: 81.4 % / Redundancy: 3.6 % / Net I/σ(I): 20.8 |
-Processing
Software |
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Refinement | Resolution: 2.494→35.051 Å / SU ML: 0.34 / Cross valid method: FREE R-VALUE / σ(F): 1.38 / Phase error: 29.37
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.494→35.051 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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