WASP homologue-associated protein with actin, membranes and microtubules / JMY/WHAMM, middle domain / JMY/WHAMM, N-terminal / Junction-mediating and -regulatory protein / N-terminal of Junction-mediating and WASP homolog-associated / WH2 domain / WH2 domain profile. 類似検索 - ドメイン・相同性
WASP homolog-associated protein with actin, membranes and microtubules 類似検索 - 構成要素
ジャーナル: J Mol Biol / 年: 2017 タイトル: Structural Insights of WHAMM's Interaction with Microtubules by Cryo-EM. 著者: Tianyang Liu / Anbang Dai / Yong Cao / Rui Zhang / Meng-Qiu Dong / Hong-Wei Wang / 要旨: WASP homolog associated with actin, membranes, and microtubules (WHAMM) is a vertebrate protein functioning in membrane tubulation for intracellular membrane trafficking and specific organelle ...WASP homolog associated with actin, membranes, and microtubules (WHAMM) is a vertebrate protein functioning in membrane tubulation for intracellular membrane trafficking and specific organelle formation. Composed of multiple domains, WHAMM can bind to membrane and microtubule (MT) and promote actin polymerization nucleation. Previous work revealed that WHAMM's activity to promote actin nucleation is repressed upon binding to MTs. Here, we discovered that WHAMM interacts with αβ-tubulin through a small peptide motif within its MT-binding domain. We reconstructed a high-resolution structure of WHAMM's MT-binding motif (MBM) assembling around MTs using cryo-electron microscopy and verified it with chemical cross-linking and mass spectrometry analysis. We also detected a conformational switch of this motif between the non-MT-bound state and the MT-bound state. These discoveries provide new insights into the mechanism by which WHAMM coordinates actin and MT networks, the two major cytoskeletal systems involved in membrane trafficking and membrane remodeling.
WASPhomolog-associatedproteinwithactin, membranesandmicrotubules / WAS protein homology region 2 domain-containing protein 1 / WH2 domain-containing protein 1