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Open data
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Basic information
Entry | Database: PDB / ID: 5wx9 | ||||||
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Title | Crystal Structure of AtERF96 with GCC-box | ||||||
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![]() | DNA BINDING PROTEIN/DNA / Protein-DNA complex / DNA BINDING PROTEIN-DNA complex | ||||||
Function / homology | ![]() positive regulation of cellular defense response / stomatal closure / positive regulation of abscisic acid-activated signaling pathway / ethylene-activated signaling pathway / sequence-specific DNA binding / DNA-binding transcription factor activity / positive regulation of DNA-templated transcription / nucleus Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chen, C.Y. / Cheng, Y.S. | ||||||
![]() | ![]() Title: Structural insights into Arabidopsis ethylene response factor 96 with an extended N-terminal binding to GCC box. Authors: Chen, C.Y. / Lin, P.H. / Chen, K.H. / Cheng, Y.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 62.4 KB | Display | ![]() |
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PDB format | ![]() | 41.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 439.1 KB | Display | ![]() |
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Full document | ![]() | 460.6 KB | Display | |
Data in XML | ![]() | 15.4 KB | Display | |
Data in CIF | ![]() | 23 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1gccS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 14364.763 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: DNA chain | Mass: 3319.175 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
#3: DNA chain | Mass: 3390.209 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.53 Å3/Da / Density % sol: 51.47 % / Mosaicity: 0.633 ° / Mosaicity esd: 0.01 ° |
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Crystal grow | Temperature: 297 K / Method: vapor diffusion / pH: 7 Details: 0.02M Magnesium chloride hexahydrate &, 0.05M MOPS pH 7.0 &, 55% Tacsimate pH 7.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Mar 25, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97622 Å / Relative weight: 1 |
Reflection | Resolution: 1.76→50 Å / Num. obs: 20152 / % possible obs: 97.3 % / Redundancy: 3.4 % / Biso Wilson estimate: 9.01 Å2 / Rmerge(I) obs: 0.051 / Net I/σ(I): 14.3 |
Reflection shell | Resolution: 1.76→1.82 Å / Redundancy: 3.5 % / Rmerge(I) obs: 0.341 / % possible all: 98.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1GCC Resolution: 1.76→21.12 Å / SU ML: 0.15 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 18.05
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 19.18 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.76→21.12 Å
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Refine LS restraints |
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LS refinement shell |
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