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- PDB-5w0z: Crystal structure of MBP fused activation-induced cytidine deamin... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5w0z | ||||||
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Title | Crystal structure of MBP fused activation-induced cytidine deaminase (AID) | ||||||
![]() | MBP fused activation-induced cytidine deaminase | ||||||
![]() | HYDROLASE / Class switch recombination / Cytidine deaminase | ||||||
Function / homology | ![]() somatic diversification of immunoglobulins / regulation of nuclear cell cycle DNA replication / single-stranded DNA cytosine deaminase / DNA cytosine deamination / : / cytidine to uridine editing / cytidine deaminase activity / negative regulation of single stranded viral RNA replication via double stranded DNA intermediate / : / positive regulation of gene expression via chromosomal CpG island demethylation ...somatic diversification of immunoglobulins / regulation of nuclear cell cycle DNA replication / single-stranded DNA cytosine deaminase / DNA cytosine deamination / : / cytidine to uridine editing / cytidine deaminase activity / negative regulation of single stranded viral RNA replication via double stranded DNA intermediate / : / positive regulation of gene expression via chromosomal CpG island demethylation / isotype switching / carbohydrate transmembrane transporter activity / maltose binding / maltose transport / maltodextrin transmembrane transport / somatic hypermutation of immunoglobulin genes / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / B cell differentiation / Chromatin modifications during the maternal to zygotic transition (MZT) / P-body / mRNA processing / outer membrane-bounded periplasmic space / cellular response to lipopolysaccharide / defense response to virus / defense response to bacterium / ubiquitin protein ligase binding / protein-containing complex / RNA binding / zinc ion binding / identical protein binding / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Qiao, Q. / Wang, L. / Wu, H. | ||||||
![]() | ![]() Title: AID Recognizes Structured DNA for Class Switch Recombination. Authors: Qiao, Q. / Wang, L. / Meng, F.L. / Hwang, J.K. / Alt, F.W. / Wu, H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 221.6 KB | Display | ![]() |
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PDB format | ![]() | 176.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 440.7 KB | Display | ![]() |
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Full document | ![]() | 446.9 KB | Display | |
Data in XML | ![]() | 37.1 KB | Display | |
Data in CIF | ![]() | 50.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5w0rSC ![]() 5w0uC ![]() 5w1cC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: _ / Ens-ID: 1 / Beg auth comp-ID: ILE / Beg label comp-ID: ILE / End auth comp-ID: GLN / End label comp-ID: GLN / Refine code: _ / Auth seq-ID: 3 - 1181 / Label seq-ID: 3 - 549
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Components
#1: Protein | Mass: 61717.914 Da / Num. of mol.: 2 Fragment: UNP P0AEY0 residues 27-392,UNP Q9GZX7 residues 13-181 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: malE, Z5632, ECs5017, AICDA, AID / Production host: ![]() ![]() References: UniProt: P0AEY0, UniProt: Q9GZX7, single-stranded DNA cytosine deaminase #2: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50.72 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6 / Details: 0.26 M NaCl, 0.1 M MES pH 6.0, 12% PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Mar 20, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 3.6→188.3 Å / Num. obs: 12574 / % possible obs: 99.8 % / Redundancy: 7.9 % / Net I/σ(I): 6.4 |
Reflection shell | Resolution: 3.61→3.74 Å / Redundancy: 8.1 % / Mean I/σ(I) obs: 1 / Num. unique obs: 11572 / CC1/2: 0.688 / Rpim(I) all: 0.743 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5W0R Resolution: 3.61→25 Å / Cor.coef. Fo:Fc: 0.855 / Cor.coef. Fo:Fc free: 0.821 / SU B: 60.783 / SU ML: 0.85 / Cross valid method: THROUGHOUT / ESU R Free: 0.967 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 64.687 Å2
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Refinement step | Cycle: 1 / Resolution: 3.61→25 Å
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Refine LS restraints |
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