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- PDB-5v0t: Crystal structure of an alpha,alpha-trehalose-phosphate synthase ... -

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Basic information

Entry
Database: PDB / ID: 5v0t
TitleCrystal structure of an alpha,alpha-trehalose-phosphate synthase (UDP-forming) from Burkholderia xenovorans in complex with glucose-6-phosphate
ComponentsAlpha,alpha-trehalose-phosphate synthase (UDP-forming)Alpha,alpha-trehalose-phosphate synthase (UDP-forming)
KeywordsTRANSFERASE / SSGCID / Burkholderia xenovorans / alpha / alpha-trehalose-phosphate synthase / UDP / glucose-6-phosphate / Structural Genomics / Seattle Structural Genomics Center for Infectious Disease
Function / homology
Function and homology information


alpha,alpha-trehalose-phosphate synthase (UDP-forming) / alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity / trehalose biosynthetic process / nucleotide binding
Similarity search - Function
Alpha,alpha-trehalose-phosphate synthase / Glycosyl transferase, family 20 / Glycosyltransferase family 20 / Glycogen Phosphorylase B; / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
ACETATE ION / FORMIC ACID / 6-O-phosphono-alpha-D-glucopyranose / OXALIC ACID / D(-)-TARTARIC ACID / URIDINE-5'-DIPHOSPHATE / Trehalose-6-phosphate synthase
Similarity search - Component
Biological speciesBurkholderia xenovorans (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.95 Å
AuthorsSeattle Structural Genomics Center for Infectious Disease (SSGCID)
CitationJournal: to be published
Title: Crystal structure of an alpha,alpha-trehalose-phosphate synthase (UDP-forming) from Burkholderia xenovorans in complex with glucose-6-phosphate
Authors: Mayclin, S.J. / Dranow, D.M. / Lorimer, D. / Edwards, T.E.
History
DepositionFeb 28, 2017Deposition site: RCSB / Processing site: RCSB
Revision 1.0Apr 5, 2017Provider: repository / Type: Initial release
Revision 1.1Jul 29, 2020Group: Data collection / Derived calculations / Structure summary
Category: chem_comp / entity ...chem_comp / entity / pdbx_chem_comp_identifier / pdbx_entity_nonpoly / struct_site / struct_site_gen
Item: _chem_comp.mon_nstd_flag / _chem_comp.name ..._chem_comp.mon_nstd_flag / _chem_comp.name / _chem_comp.type / _entity.pdbx_description / _pdbx_entity_nonpoly.name
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 1.2Oct 4, 2023Group: Data collection / Database references ...Data collection / Database references / Refinement description / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)
B: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)
C: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)
D: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)
E: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)
F: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)
G: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)
H: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)450,42187
Polymers441,4478
Non-polymers8,97479
Water51,3432850
1
A: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)56,45414
Polymers55,1811
Non-polymers1,27313
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)56,69318
Polymers55,1811
Non-polymers1,51217
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)56,2109
Polymers55,1811
Non-polymers1,0308
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)56,27111
Polymers55,1811
Non-polymers1,09110
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
5
E: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)56,45312
Polymers55,1811
Non-polymers1,27211
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
6
F: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)56,25310
Polymers55,1811
Non-polymers1,0739
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
7
G: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)55,9665
Polymers55,1811
Non-polymers7854
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
8
H: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)56,1208
Polymers55,1811
Non-polymers9407
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)84.920, 105.950, 135.710
Angle α, β, γ (deg.)91.360, 90.370, 89.970
Int Tables number1
Space group name H-MP1

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Components

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Protein / Sugars , 2 types, 16 molecules ABCDEFGH

#1: Protein
Alpha,alpha-trehalose-phosphate synthase (UDP-forming) / Alpha,alpha-trehalose-phosphate synthase (UDP-forming) / Osmoregulatory trehalose synthesis protein A / Trehalose-6-phosphate synthase


Mass: 55180.852 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Burkholderia xenovorans (strain LB400) (bacteria)
Strain: LB400 / Gene: Bxe_A1242 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)
References: UniProt: Q13W28, alpha,alpha-trehalose-phosphate synthase (UDP-forming)
#3: Sugar
ChemComp-G6P / 6-O-phosphono-alpha-D-glucopyranose / ALPHA-D-GLUCOSE-6-PHOSPHATE / 6-O-phosphono-alpha-D-glucose / 6-O-phosphono-D-glucose / 6-O-phosphono-glucose


Type: D-saccharide, alpha linking / Mass: 260.136 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Formula: C6H13O9P
IdentifierTypeProgram
a-D-Glcp6PO3IUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0

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Non-polymers , 7 types, 2921 molecules

#2: Chemical
ChemComp-UDP / URIDINE-5'-DIPHOSPHATE / Uridine diphosphate


Type: RNA linking / Mass: 404.161 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C9H14N2O12P2 / Comment: UDP*YM
#4: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol


Mass: 62.068 Da / Num. of mol.: 15 / Source method: obtained synthetically / Formula: C2H6O2
#5: Chemical
ChemComp-ACT / ACETATE ION / Acetate


Mass: 59.044 Da / Num. of mol.: 15 / Source method: obtained synthetically / Formula: C2H3O2
#6: Chemical
ChemComp-OXD / OXALIC ACID / Oxalic acid


Mass: 90.035 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C2H2O4
#7: Chemical...
ChemComp-FMT / FORMIC ACID / Formic acid


Mass: 46.025 Da / Num. of mol.: 27 / Source method: obtained synthetically / Formula: CH2O2
#8: Chemical ChemComp-TAR / D(-)-TARTARIC ACID / Tartaric acid


Mass: 150.087 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C4H6O6
#9: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 2850 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.77 Å3/Da / Density % sol: 56 %
Crystal growTemperature: 290 K / Method: vapor diffusion, sitting drop / pH: 5.5
Details: MORPHEUS G2 (269140g2): 10% w/v PEG8000, 20% v/v EG, 20mM tartarate, citrate, oxamate, acetate, formate, 100mM MES/imidazole pH 6.5: 2mM UDP, 2mM glucose-6-phosphate, 2mM MgCl2: protein conc ...Details: MORPHEUS G2 (269140g2): 10% w/v PEG8000, 20% v/v EG, 20mM tartarate, citrate, oxamate, acetate, formate, 100mM MES/imidazole pH 6.5: 2mM UDP, 2mM glucose-6-phosphate, 2mM MgCl2: protein conc 22.24mg/mL: dc: wpj5-2

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 21-ID-F / Wavelength: 0.97872 Å
DetectorType: RAYONIX MX-225 / Detector: CCD / Date: Feb 11, 2016
RadiationMonochromator: Diamond [111] / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97872 Å / Relative weight: 1
ReflectionResolution: 1.95→50 Å / Num. obs: 329972 / % possible obs: 95.7 % / Observed criterion σ(I): -3 / Redundancy: 1.994 % / Biso Wilson estimate: 24.74 Å2 / CC1/2: 0.997 / Rmerge(I) obs: 0.05 / Rrim(I) all: 0.071 / Χ2: 0.964 / Net I/σ(I): 11.68
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. unique obsCC1/2Rrim(I) all% possible all
1.95-21.9950.4221.95244500.7130.59795.3
2-2.061.9950.352.36237000.7660.49595.4
2.06-2.121.9950.2563.21230520.8590.36295.4
2.12-2.181.9950.2043.96225190.9050.28895.7
2.18-2.251.9940.1654.8217240.9320.23395.5
2.25-2.331.9950.1465.44210720.9470.20695.8
2.33-2.421.9940.1156.69204230.9670.16395.9
2.42-2.521.9940.0967.87195510.9750.13696.1
2.52-2.631.9940.0779.65188410.9830.10996
2.63-2.761.9940.06411.34180510.9890.09196.3
2.76-2.911.9950.05213.77170960.9920.07396
2.91-3.081.9950.04415.81162710.9940.06396.4
3.08-3.31.9950.03519.57152180.9960.04996.2
3.3-3.561.9940.02923141530.9960.04295.9
3.56-3.91.9940.02626.31129220.9970.03795.5
3.9-4.361.9930.02429.09116930.9970.03395.3
4.36-5.031.9930.02330.2102660.9970.03295
5.03-6.171.9910.02329.2887370.9970.03295.6
6.17-8.721.9860.02330.9366910.9980.03295.2
8.72-501.9510.02134.8635420.9970.0391.8

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassification
XSCALEdata scaling
Cootmodel building
PHENIXrefinement
PDB_EXTRACT3.22data extraction
XDSdata reduction
MOLREPphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4b14

4b14


Resolution: 1.95→50 Å / SU ML: 0.15 / Cross valid method: FREE R-VALUE / σ(F): 1.97 / Phase error: 18.86
RfactorNum. reflection% reflection
Rfree0.1777 1982 0.6 %
Rwork0.1554 --
obs0.1555 329927 95.69 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 110.22 Å2 / Biso mean: 32.7772 Å2 / Biso min: 11.08 Å2
Refinement stepCycle: final / Resolution: 1.95→50 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms28448 0 575 2866 31889
Biso mean--36.97 39.4 -
Num. residues----3628
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00730217
X-RAY DIFFRACTIONf_angle_d0.85641159
X-RAY DIFFRACTIONf_chiral_restr0.0534469
X-RAY DIFFRACTIONf_plane_restr0.0065388
X-RAY DIFFRACTIONf_dihedral_angle_d16.13717932
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 14

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
1.95-1.99880.24151280.2307234302355895
1.9988-2.05280.26791370.2193232942343195
2.0528-2.11330.21061390.1976233892352895
2.1133-2.18150.21071220.1851234822360496
2.1815-2.25940.20191020.171233882349095
2.2594-2.34990.22761510.1693234572360896
2.3499-2.45680.20581540.1623234342358896
2.4568-2.58630.20071940.1591234352362996
2.5863-2.74830.18651590.157235182367796
2.7483-2.96050.19131160.1563236072372396
2.9605-3.25830.18941400.1549235542369496
3.2583-3.72960.1451410.1412234292357096
3.7296-4.69790.141110.1215233802349195
4.6979-42.37670.14861880.1471231482333695
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.77522.8325-0.3553.06391.7795.9043-0.0110.20630.1418-0.43760.01810.2592-0.194-0.3595-0.05940.2060.0078-0.09310.19740.01180.2375-10.9422.33063.5987
21.83770.14530.08461.477-0.10931.47250.071-0.1236-0.12570.075-00.17080.1695-0.2354-0.03830.1497-0.0574-0.01550.1386-0.00040.1844-6.813518.982918.1244
30.55850.007-0.00013.9094-1.53722.42060.17920.0932-0.2823-0.5713-0.1108-0.00130.860.1243-0.11630.22080.0039-0.03220.1508-0.04910.2623.46212.53936.8544
40.81490.0717-0.19641.8417-0.09091.1520.01890.09410.0855-0.1255-0.0286-0.0373-0.0745-0.04230.02960.10940.0131-0.00820.1156-0.00220.15935.436945.05954.4463
51.86770.10750.67682.3228-0.30882.15060.05910.2186-0.1215-0.2284-0.1058-0.16260.18380.28740.02770.17690.03280.04040.1535-0.02780.169111.418331.6688-0.9124
61.08860.3153-0.02511.6653-0.16441.2392-0.00120.2495-0.2193-0.4285-0.0296-0.19470.22590.06450.03640.24980.05990.03880.1828-0.03430.20319.385225.8664-4.2824
71.667-0.1657-0.13761.4893-0.06861.65590.05520.0770.11830.00420.01260.2358-0.16-0.2469-0.03230.14260.04980.02270.13460.00960.2226-49.736186.7259-15.2704
80.80210.0445-0.27864.1011-1.75592.81120.1073-0.09590.18660.5807-0.04050.0774-0.59980.0482-0.10450.08550.00060.00810.165-0.04470.2172-40.262185.6377-2.3086
90.75520.1213-0.01881.5405-0.08230.93220.0148-0.11150.0260.1773-0.0453-0.0916-0.0490.04070.02370.111-0.00960.00580.13430.00440.1518-34.569668.4697-1.276
103.1224-0.6071-0.5012.1782-0.77272.2028-0.1453-0.2223-0.17340.17330.0524-0.04510.0920.08440.02580.2672-0.0243-0.02620.1302-0.03030.1792-22.54120.123-16.4197
112.4107-0.31240.02253.7777-1.8912.87820.0844-0.00250.16440.103-0.4218-0.7356-0.12860.52280.17790.2326-0.0404-0.02940.21950.00160.3089-11.537926.0618-19.594
122.182-0.3979-0.05011.6457-0.36971.29360.0460.3351-0.0517-0.3342-0.0107-0.07690.2745-0.0674-0.02430.2973-0.03960.00490.1651-0.05050.1384-27.486423.7725-33.105
130.6807-0.044-0.30041.69611.98715.3306-0.08610.3042-0.4084-0.26820.01370.09271.0207-0.411-0.04820.5032-0.1164-0.0440.3233-0.10540.2965-36.757111.5042-33.0625
141.7146-0.7594-0.27074.2530.53491.9991-0.0434-0.3444-0.05040.53430.3033-0.26260.36640.0583-0.07970.2649-0.0201-0.05250.24690.02230.1804-36.587229.88414.1306
151.7677-0.3518-0.85431.64820.25231.99510.0370.00830.11660.06450.03920.080.0806-0.2605-0.07840.1636-0.0475-0.01340.15540.00960.1691-43.621241.7357-11.0833
160.9033-0.1239-0.2021.63280.06490.92380.03050.01770.0114-0.00430.0179-0.07370.0851-0.0816-0.08620.1338-0.0352-0.01260.11910.00890.1633-39.20541.1454-10.5986
171.5665-0.5058-0.1410.6628-0.43451.81270.06940.016-0.2136-0.03920.03570.05270.3206-0.2673-0.02020.2377-0.1057-0.02340.17250.02270.1939-47.218726.8681-11.8588
181.4861-0.477-0.67080.3026-0.21170.9293-0.12810.0414-0.36180.13770.01960.14470.3087-0.20640.08320.2693-0.1206-0.02660.2053-0.0020.2565-45.719920.3389-11.4534
191.84250.18420.12051.7531-0.60451.4866-0.003-0.16930.08210.2217-0.0388-0.2078-0.2276-0.00870.01820.26760.0383-0.0270.1151-0.05560.186617.632782.610829.6928
201.0806-0.10130.29421.56781.50756.5465-0.1365-0.37530.46170.35940.0770.0799-0.868-0.4671-0.04520.50310.13770.03350.3098-0.11690.3115.631394.591634.0438
211.35560.18560.07763.78790.4791.4299-0.04550.26940.066-0.56750.1982-0.0633-0.31540.0184-0.04210.30320.01750.05290.25340.02920.2075.588276.1051-3.4143
221.30890.13390.49171.68370.19191.43720.02510.0024-0.0471-0.03850.01570.0099-0.0593-0.1612-0.06510.13990.03830.02190.13080.00210.16091.050662.015212.2433
230.99560.04990.30250.9063-0.13760.6907-0.0178-0.03470.20190.0110.01730.0276-0.2412-0.21560.03440.23310.08830.02040.17630.00450.2012-2.763281.335512.1535
241.88130.0368-0.26842.24770.10961.38230.0285-0.09570.13230.1382-0.0047-0.2705-0.04560.3648-0.01940.1238-0.0007-0.00730.28890.01360.178635.00530.298627.5895
250.908-0.6037-1.1421.56091.27926.2747-0.1585-0.1227-0.08540.27980.034-0.15080.78820.55050.06140.0961-0.0261-0.04210.23850.03580.205128.100119.794838.5152
260.9268-0.3087-0.09171.1502-0.04161.277-0.0239-0.0879-0.09070.12860.00680.00740.148-0.04620.01730.1486-0.0335-0.01370.18040.00370.119814.920627.345645.7167
271.5933-0.01340.25692.1423-0.03751.3376-0.00290.0359-0.1184-0.0893-0.0221-0.28050.00090.37930.0050.1184-0.02330.01580.33970.04060.2092-7.513976.9013-25.4935
281.05750.30590.29050.96370.07111.2818-0.05990.12080.0906-0.19090.0102-0.0285-0.16620.07020.05130.16590.00580.02320.19880.02290.1357-25.514979.1911-45.3795
297.344-0.92491.71461.74912.29354.6519-0.04860.60050.2082-0.65380.08040.4017-0.4437-0.40940.10380.4899-0.1184-0.21070.76390.20960.4903-66.054245.2626-53.0427
301.13620.5612-0.06731.3503-0.59411.48880.0150.0676-0.0133-0.32570.15650.2410.2605-0.5434-0.05280.2546-0.1178-0.11740.41430.03180.1944-51.020541.8703-48.9418
316.25161-0.97281.94021.89722.5289-0.0394-0.9635-0.09070.83810.10160.34180.4121-0.22030.14510.51890.16460.19430.9190.21920.4834-23.056161.666255.0423
321.0875-0.69860.12181.3778-0.72761.65760.0004-0.1105-0.00080.40220.19510.2469-0.3465-0.675-0.02230.27020.15110.13590.47310.02660.1999-9.249664.110949.7908
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 10 through 43 )A10 - 43
2X-RAY DIFFRACTION2chain 'A' and (resid 44 through 209 )A44 - 209
3X-RAY DIFFRACTION3chain 'A' and (resid 210 through 252 )A210 - 252
4X-RAY DIFFRACTION4chain 'A' and (resid 253 through 379 )A253 - 379
5X-RAY DIFFRACTION5chain 'A' and (resid 380 through 419 )A380 - 419
6X-RAY DIFFRACTION6chain 'A' and (resid 420 through 467 )A420 - 467
7X-RAY DIFFRACTION7chain 'B' and (resid 10 through 209 )B10 - 209
8X-RAY DIFFRACTION8chain 'B' and (resid 210 through 266 )B210 - 266
9X-RAY DIFFRACTION9chain 'B' and (resid 267 through 467 )B267 - 467
10X-RAY DIFFRACTION10chain 'C' and (resid 10 through 50 )C10 - 50
11X-RAY DIFFRACTION11chain 'C' and (resid 51 through 78 )C51 - 78
12X-RAY DIFFRACTION12chain 'C' and (resid 79 through 210 )C79 - 210
13X-RAY DIFFRACTION13chain 'C' and (resid 211 through 242 )C211 - 242
14X-RAY DIFFRACTION14chain 'C' and (resid 243 through 266 )C243 - 266
15X-RAY DIFFRACTION15chain 'C' and (resid 267 through 318 )C267 - 318
16X-RAY DIFFRACTION16chain 'C' and (resid 319 through 379 )C319 - 379
17X-RAY DIFFRACTION17chain 'C' and (resid 380 through 419 )C380 - 419
18X-RAY DIFFRACTION18chain 'C' and (resid 420 through 467 )C420 - 467
19X-RAY DIFFRACTION19chain 'D' and (resid 10 through 210 )D10 - 210
20X-RAY DIFFRACTION20chain 'D' and (resid 211 through 242 )D211 - 242
21X-RAY DIFFRACTION21chain 'D' and (resid 243 through 266 )D243 - 266
22X-RAY DIFFRACTION22chain 'D' and (resid 267 through 355 )D267 - 355
23X-RAY DIFFRACTION23chain 'D' and (resid 356 through 467 )D356 - 467
24X-RAY DIFFRACTION24chain 'E' and (resid 10 through 209 )E10 - 209
25X-RAY DIFFRACTION25chain 'E' and (resid 210 through 266 )E210 - 266
26X-RAY DIFFRACTION26chain 'E' and (resid 267 through 466 )E267 - 466
27X-RAY DIFFRACTION27chain 'F' and (resid 10 through 229 )F10 - 229
28X-RAY DIFFRACTION28chain 'F' and (resid 230 through 467 )F230 - 467
29X-RAY DIFFRACTION29chain 'G' and (resid 10 through 50 )G10 - 50
30X-RAY DIFFRACTION30chain 'G' and (resid 51 through 467 )G51 - 467
31X-RAY DIFFRACTION31chain 'H' and (resid 11 through 43 )H11 - 43
32X-RAY DIFFRACTION32chain 'H' and (resid 44 through 467 )H44 - 467

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