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Yorodumi- PDB-5uvr: The core region of PilO from the type IV pilus system of Pseudomo... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5uvr | ||||||
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Title | The core region of PilO from the type IV pilus system of Pseudomonas aeruginosa | ||||||
Components | PilO protein | ||||||
Keywords | MEMBRANE PROTEIN / alignment subcomplex / modified ferredoxin fold / reductive methylation / type iv pili | ||||||
Function / homology | Function and homology information oligosaccharyl transferase activity / type IV pilus assembly / type IV pilus-dependent motility / membrane Similarity search - Function | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.7 Å | ||||||
Authors | Howell, P.L. / Junop, M.S. | ||||||
Funding support | Canada, 1items
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Citation | Journal: Sci Rep / Year: 2018 Title: Conserved, unstructured regions in Pseudomonas aeruginosa PilO are important for type IVa pilus function. Authors: Leighton, T.L. / Mok, M.C. / Junop, M.S. / Howell, P.L. / Burrows, L.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5uvr.cif.gz | 35.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5uvr.ent.gz | 22.4 KB | Display | PDB format |
PDBx/mmJSON format | 5uvr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uv/5uvr ftp://data.pdbj.org/pub/pdb/validation_reports/uv/5uvr | HTTPS FTP |
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-Related structure data
Related structure data | 2rjzS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 10813.502 Da / Num. of mol.: 1 / Fragment: core region (UNP residues 109-206) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) Gene: pilO, PAERUG_E15_London_28_01_14_03389, PAERUG_P32_London_17_VIM_2_10_11_01500 Production host: Escherichia coli BL21 (bacteria) / References: UniProt: Q51353 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.78 Å3/Da / Density % sol: 55.82 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop Details: 0.2 M NaCl, 0.1 M CAPS pH 10.5, 20% (v/v) PEG 8000), 3% (v/v) DMSO |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: CLSI / Beamline: 08ID-1 / Wavelength: 0.979 Å |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Oct 25, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→35.342 Å / Num. obs: 14759 / % possible obs: 99.9 % / Redundancy: 6.7 % / Net I/σ(I): 10.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2RJZ Resolution: 1.7→35.342 Å / SU ML: 0.15 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 26.64
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→35.342 Å
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Refine LS restraints |
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LS refinement shell |
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