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- PDB-5urw: Structure of the extended type VI secretion system sheath in Myxo... -

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Entry
Database: PDB / ID: 5urw
TitleStructure of the extended type VI secretion system sheath in Myxococcus xanthus
DescriptorTssB, TssC, Hcp
KeywordsPROTEIN TRANSPORT / T6SS / type IV secretion / protein machine / secretion system / sheath / contractile
Specimen sourceMyxococcus xanthus (strain dk 1622) / bacteria
Myxococcus xanthus / bacteria / ミクソコッカス・キサンタス
MethodElectron microscopy (24 A resolution / Subtomogram averaging)
AuthorsChang, Y.-W. / Rettberg, L.A. / Jensen, G.J.
CitationEMBO Rep., 2017

EMBO Rep., 2017 StrPapers
In vivo structures of an intact type VI secretion system revealed by electron cryotomography.
Yi-Wei Chang / Lee A Rettberg / Davi R Ortega / Grant J Jensen

DateDeposition: Feb 13, 2017 / Release: May 10, 2017 / Last modification: May 24, 2017

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Assembly

Deposited unit
1A: TssB
1B: TssC
1C: TssB
1D: TssC
1E: TssB
1F: TssC
1G: TssB
1H: TssC
1I: TssB
1J: TssC
1K: TssB
1L: TssC
11: Hcp
12: Hcp
13: Hcp
14: Hcp
15: Hcp
16: Hcp
2A: TssB
2B: TssC
2C: TssB
2D: TssC
2E: TssB
2F: TssC
2G: TssB
2H: TssC
2I: TssB
2J: TssC
2K: TssB
2L: TssC
21: Hcp
22: Hcp
23: Hcp
24: Hcp
25: Hcp
26: Hcp
3A: TssB
3B: TssC
3C: TssB
3D: TssC
3E: TssB
3F: TssC
3G: TssB
3H: TssC
3I: TssB
3J: TssC
3K: TssB
3L: TssC
31: Hcp
32: Hcp
33: Hcp
34: Hcp
35: Hcp
36: Hcp


Theoretical massNumber of molelcules
Total (without water)1,660,86654
Polyers1,660,86654
Non-polymers00
Water0
#1


  • idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Polypeptide(L)
TssB


Mass: 18016.617 Da / Num. of mol.: 18
Source: (natural) Myxococcus xanthus (strain dk 1622) / bacteria
References: UniProt: Q1D305
#2: Polypeptide(L)
TssC


Mass: 56188.469 Da / Num. of mol.: 18
Source: (natural) Myxococcus xanthus / bacteria / ミクソコッカス・キサンタス
References: UniProt: Q1D304
#3: Polypeptide(L)
Hcp


Mass: 18065.271 Da / Num. of mol.: 18
Source: (natural) Myxococcus xanthus / bacteria / ミクソコッカス・キサンタス
References: UniProt: Q1D303

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentReconstruction method: SUBTOMOGRAM AVERAGING

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Sample preparation

ComponentName: Type VI secretion system / Type: COMPLEX
VitrificationCryogen name: ETHANE-PROPANE

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Electron microscopy imaging

MicroscopyMicroscope model: FEI POLARA 300
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD

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Processing

3D reconstructionResolution: 24 A / Resolution method: OTHER / Number of particles: 687

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