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- PDB-5uru: Insulin with proline analog DhP at position B28 in the R6 state -

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Basic information

Entry
Database: PDB / ID: 5uru
TitleInsulin with proline analog DhP at position B28 in the R6 state
Components(Insulin Chain ...) x 2
KeywordsHORMONE / Insulin Hormone
Function / homology
Function and homology information


negative regulation of glycogen catabolic process / positive regulation of nitric oxide mediated signal transduction / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / Signaling by Insulin receptor / IRS activation / regulation of protein secretion / Insulin processing / positive regulation of peptide hormone secretion / positive regulation of respiratory burst ...negative regulation of glycogen catabolic process / positive regulation of nitric oxide mediated signal transduction / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / Signaling by Insulin receptor / IRS activation / regulation of protein secretion / Insulin processing / positive regulation of peptide hormone secretion / positive regulation of respiratory burst / negative regulation of acute inflammatory response / Regulation of gene expression in beta cells / alpha-beta T cell activation / positive regulation of dendritic spine maintenance / Synthesis, secretion, and deacylation of Ghrelin / negative regulation of respiratory burst involved in inflammatory response / activation of protein kinase B activity / negative regulation of protein secretion / negative regulation of gluconeogenesis / positive regulation of insulin receptor signaling pathway / positive regulation of glycogen biosynthetic process / fatty acid homeostasis / Signal attenuation / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / negative regulation of lipid catabolic process / positive regulation of lipid biosynthetic process / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / regulation of protein localization to plasma membrane / nitric oxide-cGMP-mediated signaling / transport vesicle / COPI-mediated anterograde transport / positive regulation of nitric-oxide synthase activity / Insulin receptor recycling / negative regulation of reactive oxygen species biosynthetic process / positive regulation of brown fat cell differentiation / insulin-like growth factor receptor binding / NPAS4 regulates expression of target genes / neuron projection maintenance / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of mitotic nuclear division / Insulin receptor signalling cascade / positive regulation of glycolytic process / positive regulation of cytokine production / endosome lumen / positive regulation of long-term synaptic potentiation / acute-phase response / positive regulation of protein secretion / positive regulation of D-glucose import / insulin receptor binding / positive regulation of cell differentiation / Regulation of insulin secretion / wound healing / positive regulation of neuron projection development / hormone activity / negative regulation of protein catabolic process / regulation of synaptic plasticity / positive regulation of protein localization to nucleus / Golgi lumen / vasodilation / cognition / glucose metabolic process / insulin receptor signaling pathway / cell-cell signaling / glucose homeostasis / regulation of protein localization / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of cell growth / protease binding / secretory granule lumen / positive regulation of canonical NF-kappaB signal transduction / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of MAPK cascade / positive regulation of cell migration / G protein-coupled receptor signaling pathway / endoplasmic reticulum lumen / Amyloid fiber formation / Golgi membrane / negative regulation of gene expression / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of DNA-templated transcription / extracellular space / extracellular region / identical protein binding
Similarity search - Function
Insulin / Insulin family / Insulin-like / Insulin/IGF/Relaxin family / Insulin / insulin-like growth factor / relaxin family. / Insulin, conserved site / Insulin family signature. / Insulin-like superfamily
Similarity search - Domain/homology
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.41 Å
AuthorsLieblich, S.A. / Fang, K.Y. / Tirrell, D.A.
Funding support Denmark, 1items
OrganizationGrant numberCountry
Novo Nordisk Foundation Denmark
CitationJournal: To Be Published
Title: Insulin with proline analog DhP at position B28 in the R6 state
Authors: Lieblich, S.A. / Fang, K.Y. / Tirrell, D.A.
History
DepositionFeb 13, 2017Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 21, 2018Provider: repository / Type: Initial release
Revision 1.1Jan 15, 2020Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.2Oct 4, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn / struct_conn_type / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_conn.ptnr2_symmetry / _struct_conn_type.id / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id
Revision 1.3Nov 15, 2023Group: Data collection / Derived calculations / Category: chem_comp_atom / chem_comp_bond / struct_conn
Item: _chem_comp_atom.atom_id / _chem_comp_bond.atom_id_1 ..._chem_comp_atom.atom_id / _chem_comp_bond.atom_id_1 / _chem_comp_bond.atom_id_2 / _struct_conn.pdbx_leaving_atom_flag

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Insulin Chain A
B: Insulin Chain B
C: Insulin Chain A
D: Insulin Chain B
E: Insulin Chain A
F: Insulin Chain B
G: Insulin Chain A
H: Insulin Chain B
hetero molecules


Theoretical massNumber of molelcules
Total (without water)24,04220
Polymers23,2638
Non-polymers78012
Water1086
1
A: Insulin Chain A
B: Insulin Chain B
C: Insulin Chain A
D: Insulin Chain B
hetero molecules

A: Insulin Chain A
B: Insulin Chain B
C: Insulin Chain A
D: Insulin Chain B
hetero molecules

A: Insulin Chain A
B: Insulin Chain B
C: Insulin Chain A
D: Insulin Chain B
hetero molecules


Theoretical massNumber of molelcules
Total (without water)36,06430
Polymers34,89412
Non-polymers1,17018
Water1086
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation2_665-y+1,x-y+1,z1
crystal symmetry operation3_565-x+y,-x+1,z1
Buried area19320 Å2
ΔGint-473.2 kcal/mol
Surface area10730 Å2
MethodPISA
2
E: Insulin Chain A
F: Insulin Chain B
G: Insulin Chain A
H: Insulin Chain B
hetero molecules

E: Insulin Chain A
F: Insulin Chain B
G: Insulin Chain A
H: Insulin Chain B
hetero molecules

E: Insulin Chain A
F: Insulin Chain B
G: Insulin Chain A
H: Insulin Chain B
hetero molecules


Theoretical massNumber of molelcules
Total (without water)36,06430
Polymers34,89412
Non-polymers1,17018
Water1629
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation2_665-y+1,x-y+1,z1
crystal symmetry operation3_565-x+y,-x+1,z1
Buried area20300 Å2
ΔGint-727.4 kcal/mol
Surface area9810 Å2
MethodPISA
Unit cell
Length a, b, c (Å)79.259, 79.259, 79.874
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number146
Space group name H-MH3
Components on special symmetry positions
IDModelComponents
11B-101-

ZN

21B-102-

CL

31D-101-

ZN

41D-102-

CL

51F-101-

ZN

61F-102-

CL

71H-101-

ZN

81H-102-

CL

91F-201-

HOH

Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11A
21C
12A
22E
13A
23G
14B
24D
15B
25F
16B
26H
17C
27E
18C
28G
19D
29F
110D
210H
111E
211G
112F
212H

NCS domain segments:

Component-ID: 1 / Refine code: 1

Dom-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11GLYGLYASNASNAA1 - 211 - 21
21GLYGLYASNASNCC1 - 211 - 21
12GLYGLYASNASNAA1 - 211 - 21
22GLYGLYASNASNEE1 - 211 - 21
13GLYGLYASNASNAA1 - 211 - 21
23GLYGLYASNASNGG1 - 211 - 21
14VALVALTHRTHRBB2 - 272 - 27
24VALVALTHRTHRDD2 - 272 - 27
15VALVALTHRTHRBB2 - 272 - 27
25VALVALTHRTHRFF2 - 272 - 27
16PHEPHE8LJ8LJBB1 - 281 - 28
26PHEPHE8LJ8LJHH1 - 281 - 28
17GLYGLYASNASNCC1 - 211 - 21
27GLYGLYASNASNEE1 - 211 - 21
18GLYGLYASNASNCC1 - 211 - 21
28GLYGLYASNASNGG1 - 211 - 21
19VALVAL8LJ8LJDD2 - 282 - 28
29VALVAL8LJ8LJFF2 - 282 - 28
110VALVALTHRTHRDD2 - 272 - 27
210VALVALTHRTHRHH2 - 272 - 27
111GLYGLYASNASNEE1 - 211 - 21
211GLYGLYASNASNGG1 - 211 - 21
112VALVALTHRTHRFF2 - 272 - 27
212VALVALTHRTHRHH2 - 272 - 27

NCS ensembles :
ID
1
2
3
4
5
6
7
8
9
10
11
12

NCS oper:
IDCodeMatrixVector
1given(1, 1), (1, 1), (1)1
2given(2, 0.905032, 0.422339), (2, 0.423438, -0.905831), (2, 0.040225, 0.033159)0.050477, -0.013021, -0.99864
3given(1, 1), (1, 1), (1)1
4given(2, 0.996365, 0.081633), (2, -0.08136, 0.996613), (2, 0.025232, -0.009966)-0.024336, 0.011989, 0.999632
5given(1, 1), (1, 1), (1)1
6given(2, 0.870404, 0.490494), (2, 0.491373, -0.870855), (2, 0.030796, 0.032052)0.042569, -0.012793, -0.999012
7given(1, 1), (1, 1), (1)1
8given(2, 0.864889, 0.501028), (2, 0.501105, -0.865368), (2, -0.029332, -0.010464)-0.030627, -0.005646, -0.999515
9given(1, 1), (1, 1), (1)1
10given(2, 0.996011, 0.089172), (2, -0.089183, 0.99601), (2, -0.003, -0.003661)0.003315, 0.003379, 0.999989
11given(1, 1), (1, 1), (1)1
12given(2, 0.807434, 0.589305), (2, 0.58963, -0.80766), (2, -0.019665, -0.020103)-0.027736, 0.004643, -0.999605
13given(1, 1), (1, 1), (1)1
14given(2, 0.868059, 0.495886), (2, 0.495728, -0.868387), (2, 0.026981, 0.000926)0.023889, 0.012575, -0.999636
15given(1, 1), (1, 1), (1)1
16given(2, 0.997444, 0.070969), (2, -0.070976, 0.997478), (2, 0.008268, 0.00124)-0.008335, -0.00065, 0.999965
17given(1, 1), (1, 1), (1)1
18given(2, 0.818119, 0.574538), (2, 0.574521, -0.818447), (2, -0.02466, -0.007092)-0.024257, -0.008366, -0.999671
19given(1, 1), (1, 1), (1)1
20given(2, 0.994282, 0.106581), (2, -0.106494, 0.994237), (2, -0.00789, 0.011575)0.006611, -0.012349, 0.999902
21given(1, 1), (1, 1), (1)1
22given(2, 0.829059, 0.558836), (2, 0.558914, -0.829224), (2, 0.016617, 0.009444)0.019058, 0.001456, -0.999817
23given(1, 1), (1, 1), (1)1
24given(2, 0.75009, 0.660746), (2, 0.661164, -0.750189), (2, -0.015083, -0.025129)-0.02793, 0.008884, -0.99957

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Components

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Insulin Chain ... , 2 types, 8 molecules ACEGBDFH

#1: Protein/peptide
Insulin Chain A


Mass: 2383.698 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: INS / Production host: Escherichia coli (E. coli) / References: UniProt: P01308
#2: Protein/peptide
Insulin Chain B


Mass: 3431.938 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: INS / Production host: Escherichia coli (E. coli) / References: UniProt: P01308

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Non-polymers , 4 types, 18 molecules

#3: Chemical
ChemComp-IPH / PHENOL


Mass: 94.111 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C6H6O
#4: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Zn
#5: Chemical
ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Cl
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.03 Å3/Da / Density % sol: 39.27 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8
Details: 300mM Tris pH 8, 17mM Zinc Acetate, 1% Phenol, 313mM Sodium Citrate, 11.25% Acetone
Temp details: Room temperature

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SSRL / Beamline: BL12-2 / Wavelength: 0.9795 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 23, 2016
RadiationMonochromator: LIQUID NITROGEN-COOLED DOUBLE CRYSTAL K-B FOCUSING MIRRORS
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9795 Å / Relative weight: 1
ReflectionResolution: 1.68→52.06 Å / Num. obs: 18094 / % possible obs: 84.1 % / Redundancy: 4.6 % / CC1/2: 0.998 / Rmerge(I) obs: 0.087 / Rpim(I) all: 0.042 / Rrim(I) all: 0.097 / Net I/σ(I): 5.4 / Num. measured all: 82670 / Scaling rejects: 51
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. measured allNum. unique allRpim(I) allRrim(I) all% possible allCC1/2Net I/σ(I) obs
1.68-1.711.11.2654511.261.7824.5
9.02-34.525.80.0437981370.020.04798.30.99822.4

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Phasing

PhasingMethod: molecular replacement
Phasing MR
Highest resolutionLowest resolution
Rotation4.17 Å34.52 Å
Translation4.17 Å34.52 Å

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Processing

Software
NameVersionClassification
REFMACrefinement
Aimless0.3.11data scaling
PHASER2.5.6phasing
PDB_EXTRACT3.22data extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1EV6

1ev6


Resolution: 2.41→52.06 Å / Cor.coef. Fo:Fc: 0.96 / Cor.coef. Fo:Fc free: 0.943 / WRfactor Rfree: 0.272 / WRfactor Rwork: 0.2003 / FOM work R set: 0.785 / SU B: 20.717 / SU ML: 0.225 / SU R Cruickshank DPI: 0.5636 / SU Rfree: 0.28 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.564 / ESU R Free: 0.28 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.2462 376 5.2 %RANDOM
Rwork0.1868 ---
obs0.1902 6816 99.76 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso max: 90.57 Å2 / Biso mean: 56.008 Å2 / Biso min: 18.05 Å2
Baniso -1Baniso -2Baniso -3
1-0.02 Å20.01 Å20 Å2
2--0.02 Å2-0 Å2
3----0.07 Å2
Refinement stepCycle: final / Resolution: 2.41→52.06 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms1420 0 36 6 1462
Biso mean--47.87 52.7 -
Num. residues----194
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0220.0191486
X-RAY DIFFRACTIONr_bond_other_d0.0020.021258
X-RAY DIFFRACTIONr_angle_refined_deg2.0351.9672016
X-RAY DIFFRACTIONr_angle_other_deg1.19832874
X-RAY DIFFRACTIONr_dihedral_angle_1_deg7.0335186
X-RAY DIFFRACTIONr_dihedral_angle_2_deg25.12723.45555
X-RAY DIFFRACTIONr_dihedral_angle_3_deg16.63415194
X-RAY DIFFRACTIONr_dihedral_angle_4_deg5.89153
X-RAY DIFFRACTIONr_chiral_restr0.1120.2233
X-RAY DIFFRACTIONr_gen_planes_refined0.0090.021653
X-RAY DIFFRACTIONr_gen_planes_other0.0020.02334
Refine LS restraints NCS

Dom-ID: 1 / Refine-ID: X-RAY DIFFRACTION

Ens-IDAuth asym-IDNumberTypeRms dev position (Å)Weight position
1A189TIGHT POSITIONAL0.090.09
1A139TIGHT POSITIONAL0.130.09
1A186TIGHT POSITIONAL0.320.09
1A151TIGHT THERMAL3.480.87
2A139TIGHT THERMAL2.920.87
3A151TIGHT THERMAL2.90.87
4B192TIGHT THERMAL3.120.87
5B192TIGHT THERMAL4.640.87
6B198TIGHT THERMAL2.720.87
7C139TIGHT THERMAL3.010.87
8C158TIGHT THERMAL2.910.87
9D199TIGHT THERMAL2.550.87
10D189TIGHT THERMAL2.110.87
11E139TIGHT THERMAL3.680.87
12F186TIGHT THERMAL2.970.87
LS refinement shellResolution: 2.41→2.472 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.322 26 -
Rwork0.225 469 -
all-495 -
obs--99.6 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
15.47191.7314.3395.95370.65829.36180.0374-0.3162-0.40580.1727-0.0963-0.03950.5964-0.53510.05880.0803-0.05940.03290.08880.04380.21-10.961533.823611.7201
25.11511.0225-0.83364.80820.76732.9980.1034-0.0805-0.39380.07390.1690.31390.0981-0.4939-0.27240.0073-0.0244-0.01050.14350.04820.2725-10.198340.61236.6442
34.61380.4841-1.73426.9363-0.8056.06510.1110.85610.2406-0.4456-0.11080.5268-0.1595-0.6016-0.00020.10490.0594-0.14230.39150.11490.3049-15.043352.3072-6.1254
45.5564-0.9143-2.73416.3242-0.52493.9686-0.15970.5389-0.1143-0.47390.12370.74060.1394-0.55450.0360.0522-0.0248-0.08210.1613-0.01350.1898-11.4945.6445-0.9717
56.9476-4.12911.88357.1769-2.417711.79170.2863-0.6445-0.65530.7411-0.30470.17520.7532-0.11190.01840.3099-0.13450.03440.19020.16390.2619-10.076932.8374-28.6969
66.57751.89541.22515.17080.97063.17940.1274-0.4448-0.48460.455-0.01680.31860.3633-0.2012-0.11060.0855-0.03740.04250.09330.04510.1442-9.649339.4162-33.6134
75.3255-2.1434-4.53817.7337-2.2346.53810.01160.46990.0746-0.19940.11150.46810.0343-0.6769-0.12310.030.0476-0.05530.23640.04340.3113-15.598650.3558-46.2305
84.3628-1.2989-1.25213.86581.89324.95820.03760.1780.099-0.11510.20410.42890.2317-0.5565-0.24170.0286-0.0582-0.02060.13460.06540.2295-10.888243.8989-41.8519
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1A1 - 21
2X-RAY DIFFRACTION2B1 - 27
3X-RAY DIFFRACTION3C1 - 21
4X-RAY DIFFRACTION4D2 - 27
5X-RAY DIFFRACTION5E1 - 21
6X-RAY DIFFRACTION6F2 - 27
7X-RAY DIFFRACTION7G1 - 21
8X-RAY DIFFRACTION8H1 - 27

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Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

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