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Yorodumi- PDB-5unj: Structure of Human Liver Receptor Homolog 1 in complex with PGC1a... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5unj | |||||||||||||||
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Title | Structure of Human Liver Receptor Homolog 1 in complex with PGC1a and RJW100 | |||||||||||||||
Components |
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Keywords | NUCLEAR PROTEIN / Nuclear receptor / agonist / coregulator | |||||||||||||||
Function / homology | Function and homology information Regulation of gene expression in early pancreatic precursor cells / positive regulation of mitochondrial DNA metabolic process / positive regulation of muscle tissue development / pancreas morphogenesis / calcineurin-mediated signaling / positive regulation of cellular respiration / acinar cell differentiation / positive regulation of fatty acid oxidation / tissue development / : ...Regulation of gene expression in early pancreatic precursor cells / positive regulation of mitochondrial DNA metabolic process / positive regulation of muscle tissue development / pancreas morphogenesis / calcineurin-mediated signaling / positive regulation of cellular respiration / acinar cell differentiation / positive regulation of fatty acid oxidation / tissue development / : / bile acid metabolic process / : / lncRNA binding / cellular respiration / response to muscle activity / embryo development ending in birth or egg hatching / Activation of PPARGC1A (PGC-1alpha) by phosphorylation / temperature homeostasis / homeostatic process / positive regulation of ATP biosynthetic process / response to starvation / intracellular glucose homeostasis / fatty acid oxidation / response to dietary excess / adipose tissue development / positive regulation of viral genome replication / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / energy homeostasis / brown fat cell differentiation / positive regulation of gluconeogenesis / digestion / respiratory electron transport chain / cellular response to leukemia inhibitory factor / hormone-mediated signaling pathway / mitochondrion organization / SUMOylation of transcription cofactors / nuclear receptor coactivator activity / RNA splicing / cholesterol homeostasis / gluconeogenesis / transcription coregulator binding / transcription initiation at RNA polymerase II promoter / nuclear receptor binding / transcription coregulator activity / negative regulation of smooth muscle cell proliferation / phospholipid binding / circadian regulation of gene expression / Heme signaling / SUMOylation of intracellular receptors / Transcriptional activation of mitochondrial biogenesis / regulation of circadian rhythm / PPARA activates gene expression / PML body / chromatin DNA binding / mRNA processing / Transcriptional regulation of white adipocyte differentiation / Nuclear Receptor transcription pathway / RNA polymerase II transcription regulator complex / Regulation of RUNX2 expression and activity / nuclear receptor activity / positive regulation of DNA-binding transcription factor activity / Circadian Clock / sequence-specific double-stranded DNA binding / cellular response to oxidative stress / positive regulation of cold-induced thermogenesis / regulation of cell population proliferation / DNA-binding transcription activator activity, RNA polymerase II-specific / protein-containing complex assembly / neuron apoptotic process / DNA-binding transcription factor binding / Estrogen-dependent gene expression / negative regulation of neuron apoptotic process / RNA polymerase II-specific DNA-binding transcription factor binding / sequence-specific DNA binding / transcription coactivator activity / protein stabilization / transcription cis-regulatory region binding / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / ubiquitin protein ligase binding / chromatin binding / chromatin / positive regulation of gene expression / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / DNA binding / RNA binding / zinc ion binding / nucleoplasm / nucleus / cytosol / cytoplasm Similarity search - Function | |||||||||||||||
Biological species | Homo sapiens (human) | |||||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.959 Å | |||||||||||||||
Authors | Mays, S.G. / Ortlund, E.A. | |||||||||||||||
Funding support | United States, 4items
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Citation | Journal: Mol. Pharmacol. / Year: 2017 Title: Structure and Dynamics of the Liver Receptor Homolog 1-PGC1 alpha Complex. Authors: Mays, S.G. / Okafor, C.D. / Tuntland, M.L. / Whitby, R.J. / Dharmarajan, V. / Stec, J. / Griffin, P.R. / Ortlund, E.A. | |||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5unj.cif.gz | 157.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5unj.ent.gz | 125.1 KB | Display | PDB format |
PDBx/mmJSON format | 5unj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/un/5unj ftp://data.pdbj.org/pub/pdb/validation_reports/un/5unj | HTTPS FTP |
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-Related structure data
Related structure data | 5l11S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 28330.693 Da / Num. of mol.: 1 / Fragment: unp residues 299-541 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: NR5A2, B1F, CPF, FTF / Production host: Escherichia coli (E. coli) / References: UniProt: O00482 |
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#2: Protein/peptide | Mass: 1523.854 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: Q9UBK2*PLUS |
#3: Chemical | ChemComp-RJW / ( |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.11 Å3/Da / Density % sol: 41.76 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop Details: 0.05M Sodium acetate, pH 4.6; PEG4000 14%, glycerol 15-21% |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: MAR CCD 130 mm / Detector: CCD / Date: Jun 18, 2015 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 1.95→50 Å / Num. obs: 18170 / % possible obs: 96.8 % / Redundancy: 6.1 % / Biso Wilson estimate: 32.19 Å2 / Rmerge(I) obs: 0.099 / Rpim(I) all: 0.042 / Rrim(I) all: 0.108 / Χ2: 0.819 / Net I/σ(I): 7 / Num. measured all: 111677 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Processing
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5L11 Resolution: 1.959→42.014 Å / SU ML: 0.17 / Cross valid method: FREE R-VALUE / σ(F): 1.39 / Phase error: 23.84
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 133.1 Å2 / Biso mean: 46.7829 Å2 / Biso min: 22.98 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.959→42.014 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 9
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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