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- PDB-5udv: LarE, a sulfur transferase involved in synthesis of the cofactor ... -

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Basic information

Entry
Database: PDB / ID: 5udv
TitleLarE, a sulfur transferase involved in synthesis of the cofactor for lactate racemase, in complex with iron
ComponentsLactate racemization operon protein LarE
KeywordsTRANSFERASE / Lar / sulfur transferase / LarE / AMPylation / hexamer / trimer / PP-loop / ATP pyrophophatase domain / lactate / lactate racemization / lactate racemase
Function / homology
Function and homology information


pyridinium-3,5-bisthiocarboxylic acid mononucleotide synthase / sulfurtransferase activity / lyase activity / ATP binding
Similarity search - Function
Pyridinium-3,5-bisthiocarboxylic acid mononucleotide synthase LarE / NAD/GMP synthase / NAD synthase / Rossmann-like alpha/beta/alpha sandwich fold
Similarity search - Domain/homology
: / PHOSPHATE ION / Pyridinium-3,5-bisthiocarboxylic acid mononucleotide synthase
Similarity search - Component
Biological speciesLactobacillus plantarum (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.621 Å
AuthorsFellner, M. / Desguin, B. / Hausinger, R.P. / Hu, J.
Funding support United States, 1items
OrganizationGrant numberCountry
National Science Foundation (NSF, United States) United States
CitationJournal: Proc. Natl. Acad. Sci. U.S.A. / Year: 2017
Title: Structural insights into the catalytic mechanism of a sacrificial sulfur insertase of the N-type ATP pyrophosphatase family, LarE.
Authors: Fellner, M. / Desguin, B. / Hausinger, R.P. / Hu, J.
History
DepositionDec 28, 2016Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 23, 2017Provider: repository / Type: Initial release
Revision 1.1Aug 30, 2017Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.2Nov 27, 2019Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.3Oct 4, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Lactate racemization operon protein LarE
B: Lactate racemization operon protein LarE
C: Lactate racemization operon protein LarE
D: Lactate racemization operon protein LarE
E: Lactate racemization operon protein LarE
F: Lactate racemization operon protein LarE
hetero molecules


Theoretical massNumber of molelcules
Total (without water)191,66721
Polymers190,3136
Non-polymers1,35415
Water97354
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area16430 Å2
ΔGint-247 kcal/mol
Surface area62630 Å2
MethodPISA
Unit cell
Length a, b, c (Å)107.546, 107.546, 318.289
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number91
Space group name H-MP4122
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain B and (resseq 3:5 or (resid 6 and (name...
21(chain D and (resseq 3:5 or (resid 6 and (name...
12(chain A and ((resid 3 and (name N or name...
22(chain C and (resseq 3:6 or (resid 7 and (name...
32(chain F and ((resid 3 and (name N or name...

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111THRTHRALAALA(chain B and (resseq 3:5 or (resid 6 and (name...BB3 - 53 - 5
121THRTHRTHRTHR(chain B and (resseq 3:5 or (resid 6 and (name...BB66
131ALAALAARGARG(chain B and (resseq 3:5 or (resid 6 and (name...BB2 - 2592 - 259
141ALAALAARGARG(chain B and (resseq 3:5 or (resid 6 and (name...BB2 - 2592 - 259
151ALAALAARGARG(chain B and (resseq 3:5 or (resid 6 and (name...BB2 - 2592 - 259
161ALAALAARGARG(chain B and (resseq 3:5 or (resid 6 and (name...BB2 - 2592 - 259
211THRTHRALAALA(chain D and (resseq 3:5 or (resid 6 and (name...DD3 - 53 - 5
221THRTHRTHRTHR(chain D and (resseq 3:5 or (resid 6 and (name...DD66
231THRTHRARGARG(chain D and (resseq 3:5 or (resid 6 and (name...DD3 - 2593 - 259
241THRTHRARGARG(chain D and (resseq 3:5 or (resid 6 and (name...DD3 - 2593 - 259
251THRTHRARGARG(chain D and (resseq 3:5 or (resid 6 and (name...DD3 - 2593 - 259
261THRTHRARGARG(chain D and (resseq 3:5 or (resid 6 and (name...DD3 - 2593 - 259
112THRTHRTHRTHR(chain A and ((resid 3 and (name N or name...AA33
122ALAALAALAALA(chain A and ((resid 3 and (name N or name...AA2 - 2772 - 277
132ALAALAALAALA(chain A and ((resid 3 and (name N or name...AA2 - 2772 - 277
142ALAALAALAALA(chain A and ((resid 3 and (name N or name...AA2 - 2772 - 277
152ALAALAALAALA(chain A and ((resid 3 and (name N or name...AA2 - 2772 - 277
212THRTHRTHRTHR(chain C and (resseq 3:6 or (resid 7 and (name...CC3 - 63 - 6
222LYSLYSLYSLYS(chain C and (resseq 3:6 or (resid 7 and (name...CC77
232THRTHRARGARG(chain C and (resseq 3:6 or (resid 7 and (name...CC3 - 2593 - 259
242THRTHRARGARG(chain C and (resseq 3:6 or (resid 7 and (name...CC3 - 2593 - 259
252THRTHRARGARG(chain C and (resseq 3:6 or (resid 7 and (name...CC3 - 2593 - 259
312THRTHRTHRTHR(chain F and ((resid 3 and (name N or name...FF33
322ALAALASERSER(chain F and ((resid 3 and (name N or name...FF2 - 2602 - 260
332ALAALASERSER(chain F and ((resid 3 and (name N or name...FF2 - 2602 - 260
342ALAALASERSER(chain F and ((resid 3 and (name N or name...FF2 - 2602 - 260
352ALAALASERSER(chain F and ((resid 3 and (name N or name...FF2 - 2602 - 260

NCS ensembles :
ID
1
2

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Components

#1: Protein
Lactate racemization operon protein LarE


Mass: 31718.766 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) (bacteria)
Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1 / Gene: larE, lp_0109 / Plasmid: pGIR076 / Production host: Escherichia coli (E. coli) / Strain (production host): Arctic express DE3 / References: UniProt: F9UST4
#2: Chemical ChemComp-FE / FE (III) ION


Mass: 55.845 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Fe
#3: Chemical
ChemComp-PO4 / PHOSPHATE ION


Mass: 94.971 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: PO4
#4: Chemical
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: SO4
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 54 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.42 Å3/Da / Density % sol: 49.13 % / Mosaicity: 0.23 °
Crystal growTemperature: 294.15 K / Method: vapor diffusion, hanging drop / pH: 6.5
Details: 4 ul ~39 mg/ml LarE (100 mM Tris-HCl pH 7.5, 300 mM NaCl) mixed with 4 ul of reservoir solution. Hanging drop reservoir contained 100 ul of 37.5% v/v pentaerythritol ethoxylate (15/4 EO/OH), ...Details: 4 ul ~39 mg/ml LarE (100 mM Tris-HCl pH 7.5, 300 mM NaCl) mixed with 4 ul of reservoir solution. Hanging drop reservoir contained 100 ul of 37.5% v/v pentaerythritol ethoxylate (15/4 EO/OH), 50 mM BIS-TRIS pH 6.5, 105 mM ammonium sulfate. Crystal soaked 4.5 minutes in 30.0% v/v pentaerythritol ethoxylate (15/4 EO/OH), 50 mM BIS-TRIS pH 5.5, 50 mM ammonium sulfate, 38 mM iron sulfate.

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 21-ID-G / Wavelength: 0.9786 Å
DetectorType: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Jun 22, 2016
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9786 Å / Relative weight: 1
ReflectionResolution: 2.62→47.96 Å / Num. obs: 57095 / % possible obs: 99.9 % / Redundancy: 7.9 % / Biso Wilson estimate: 55.12 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.098 / Net I/σ(I): 15.6
Reflection shell
Resolution (Å)Redundancy (%)Rmerge(I) obsCC1/2Diffraction-ID% possible all
2.62-2.698.10.8120.684199.3
11.42-47.965.90.0330.999196.6

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Phasing

PhasingMethod: molecular replacement
Phasing MR
Highest resolutionLowest resolution
Rotation8.34 Å47.58 Å
Translation8.34 Å47.58 Å

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Processing

Software
NameVersionClassification
Aimless0.5.27data scaling
PHENIX1.11.1-2575refinement
PDB_EXTRACT3.22data extraction
XDSdata reduction
PHASER2.6.0phasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5UDQ

5udq


Resolution: 2.621→47.575 Å / SU ML: 0.42 / Cross valid method: FREE R-VALUE / σ(F): 1.92 / Phase error: 26.9
RfactorNum. reflection% reflection
Rfree0.259 5198 4.86 %
Rwork0.2103 --
obs0.2127 53433 99.82 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 141.71 Å2 / Biso mean: 64.6372 Å2 / Biso min: 22.73 Å2
Refinement stepCycle: final / Resolution: 2.621→47.575 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11525 0 67 54 11646
Biso mean--56.11 46.65 -
Num. residues----1508
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0111802
X-RAY DIFFRACTIONf_angle_d0.97715981
X-RAY DIFFRACTIONf_chiral_restr0.0531861
X-RAY DIFFRACTIONf_plane_restr0.0062060
X-RAY DIFFRACTIONf_dihedral_angle_d13.5294149
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11B1929X-RAY DIFFRACTION7.702TORSIONAL
12D1929X-RAY DIFFRACTION7.702TORSIONAL
21A3063X-RAY DIFFRACTION7.702TORSIONAL
22C3063X-RAY DIFFRACTION7.702TORSIONAL
23F3063X-RAY DIFFRACTION7.702TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 30

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.6207-2.65050.3941150.3333301341698
2.6505-2.68170.41571850.326334313616100
2.6817-2.71440.36281580.328334013559100
2.7144-2.74870.4031760.31533603536100
2.7487-2.78490.37181920.310533983590100
2.7849-2.8230.38661570.319434393596100
2.823-2.86340.36711720.294433483520100
2.8634-2.90610.28781920.285934113603100
2.9061-2.95150.32711580.278433663524100
2.9515-2.99990.37282030.292534113614100
2.9999-3.05160.32441780.273233763554100
3.0516-3.10710.3031670.280433973564100
3.1071-3.16680.32961820.265234543636100
3.1668-3.23140.30581480.267333893537100
3.2314-3.30170.33491790.257133813560100
3.3017-3.37850.2881610.231234013562100
3.3785-3.46290.27131820.228434223604100
3.4629-3.55650.26131890.22733293518100
3.5565-3.66120.28151630.212334093572100
3.6612-3.77930.27112170.199333293546100
3.7793-3.91430.24141770.19334043581100
3.9143-4.07090.23611700.186633783548100
4.0709-4.25610.20341870.16733783565100
4.2561-4.48030.18842000.161534173617100
4.4803-4.76080.21481460.15633763522100
4.7608-5.1280.22771580.15734193577100
5.128-5.64330.21261850.164433743559100
5.6433-6.45820.2091460.190834073553100
6.4582-8.130.20951860.181533833569100
8.13-47.58310.23531690.1763378354799
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.30290.835-0.36886.60620.12622.7798-0.1441-0.19380.25151.74280.5095-0.6532-0.6281-0.1361-0.30760.7150.2242-0.14950.5765-0.06940.523120.95121.6742-15.2021
24.6965-0.01390.02622.6665-0.69874.42550.07930.352-0.0052-0.87980.16521.2471-0.6561-0.3321-0.2830.58270.0840.04080.64230.12970.376715.6762-13.8345-21.2241
32.3436-2-1.03896.2893-0.81041.1596-0.0367-0.2480.3995-0.0610.1573-0.56080.111-0.002-0.1330.492-0.0553-0.09610.7567-0.05240.4322.2678-7.2778-26.5835
41.70371.27260.65645.79480.64210.333-0.2228-0.4749-0.30821.82370.4394-1.7581-0.23120.3097-0.19670.89450.1712-0.4220.668-0.22380.958830.50910.2233-12.227
54.3274-1.258-0.6664.1341-1.938.21350.03790.24670.062-0.1381-0.11590.02970.307-0.1990.06430.40490.0319-0.06450.2807-0.01170.348517.8014-29.3922-4.8409
67.3676-2.82043.74946.4179-1.73585.3610.10911.696-0.05430.69410.2266-0.91030.33380.7679-0.20940.45030.1295-0.03360.5891-0.04610.275926.2299-32.4006-4.0888
74.0954-2.53610.54521.99450.75247.58210.40670.28340.0259-1.08240.5042-0.702-0.1915-1.525-0.87010.85530.04710.03471.0840.10660.857442.2536-32.9994-7.9895
86.70233.1588-1.36155.2922-1.57476.3394-0.44620.3650.73950.16420.3156-0.2249-0.2268-0.38460.21020.46780.0808-0.0060.42990.11140.6975.87199.50095.3826
99.4447-1.44530.44059.0564-1.37233.96230.15640.10330.5371-0.0498-0.3286-0.2534-0.24230.29790.23810.487-0.03220.14050.32490.11070.650711.11556.918616.6283
106.28761.4830.82656.5142-2.45469.11550.7551-0.28650.59031.4489-1.2011-0.3698-1.17830.34640.44620.33930.0298-0.0060.3965-0.05860.585611.3662.984627.0307
116.57351.4445-2.5998.4312-3.17788.59190.28760.67770.81530.1507-0.487-0.9661-0.31180.88560.15990.3538-0.1281-0.02480.60820.1080.695417.22961.627526.6067
121.997-3.0373-0.53747.3507-3.25057.6462-0.3816-0.7132-0.20610.72011.11431.4558-0.3992-1.3959-0.57770.44120.00640.00740.67990.1360.5927-1.6219-11.636134.5267
137.77763.8185-2.51814.2236-3.28356.55760.0352-0.51920.2089-0.1636-0.03350.4069-0.3432-0.5307-0.00640.44960.04660.02810.53070.01440.471-0.65680.681628.7674
145.11113.17544.07388.10615.07247.970.3141-0.4873-0.1020.689-0.08380.33870.7218-1.2634-0.05490.4734-0.00920.0830.66290.21820.65962.01522.644113.5514
158.89543.114-1.51576.767-0.18640.68970.3891.0277-0.044-0.2174-0.3551-1.27250.03550.257-0.14350.64780.2070.16910.52070.02960.749916.0174-3.488511.3684
167.1962-3.1652-2.83843.18272.42385.8837-0.03980.0647-0.2397-0.1491-0.04710.46150.1638-0.38380.10910.3865-0.0794-0.03930.25170.0480.373812.7555-21.8428.897
179.3445-4.172-6.64429.5477.28277.2631-0.2327-0.0446-0.20530.43560.0943-0.1660.4999-0.04730.16060.33920.0107-0.06220.24690.05690.284524.6126-29.146828.8226
187.84563.7035-1.61733.70062.32575.2416-0.01510.12850.075-0.24310.0858-0.10230.0106-0.9867-0.19670.3790.0384-0.07030.23980.01550.254713.5857-29.4127.7678
194.1042-3.40120.78439.3625-2.06413.5140.0852-0.3568-0.2419-0.19860.21170.1691-0.0554-0.1209-0.25430.4763-0.02160.08280.4182-0.00780.475445.34465.790512.4429
208.4796-8.77215.62259.8243-6.69384.5866-0.0204-0.797-0.7060.09160.65721.18730.0394-0.7377-0.68080.43890.0080.02030.49660.01160.644145.0223-9.537215.404
212.59882.0603-0.77384.31090.17384.22680.10920.0470.1249-0.1560.0786-0.22760.1408-0.138-0.18280.36350.0296-0.04560.27370.03840.41144.4385-25.623312.8541
222.3853-2.71120.15734.5784-0.76511.9315-0.02950.03840.0432-0.0867-0.0254-0.2290.50620.18440.05220.5922-0.06370.06870.3981-0.01120.411114.3458-67.891633.9961
239.47770.5168-0.23335.44410.53554.2498-0.0932-0.045-0.1761-0.02650.04990.16250.058-0.14190.0510.3728-0.058-0.00370.22270.04710.279311.5488-42.365522.8987
242.26211.27131.02855.95772.80472.1793-0.04180.2064-0.33770.67470.2106-0.66980.58880.3178-0.18660.58630.0922-0.03620.5073-0.0080.618253.0197-64.857424.4131
255.224-0.41610.86874.17940.36367.3968-0.06560.37-0.1096-0.30190.1159-0.23720.30020.7427-0.0510.37830.0131-0.07660.41240.0360.400144.0728-39.370621.4223
265.79142.6045-3.64051.8956-2.4697.00140.10330.2015-0.1572-0.0047-0.04730.1272-0.19440.0038-0.05040.49540.0703-0.00730.2575-0.07050.373824.5852-81.30651.5171
279.56440.912.70055.2149-5.14046.61840.10180.5778-0.1132-0.2864-0.2012-0.8173-0.74150.81330.11690.6056-0.07020.06930.3964-0.11840.53334.9099-72.5316-1.8042
281.92921.141-1.84136.7143-0.47122.3582-0.33380.28930.1741-1.54770.34520.66420.49480.0615-0.06180.76760.0127-0.18480.4218-0.08180.522221.8223-68.6407-15.5268
299.6211-1.15-2.16155.6321-2.92746.140.15230.47110.2377-0.12860.41870.8313-0.4348-0.7592-0.49380.4813-0.0217-0.05410.4014-0.07340.520815.4051-78.8598-2.2676
302.0817-0.38143.21430.0909-0.4985.125-0.2548-0.1809-0.09580.42320.0555-0.1643-1.1753-0.13410.14910.72560.03490.0760.3944-0.09950.415223.2465-72.044510.3001
316.9350.58090.84776.16614.10075.11730.26030.3044-0.4363-0.2111-0.02890.1280.7794-0.1791-0.23110.66-0.0026-0.10130.26730.03670.394127.4753-50.2107-6.4587
328.1808-1.4677-1.91124.6925-1.33281.36720.03940.1440.02-0.2902-0.0449-0.0930.3839-0.00580.03260.50760.0708-0.0720.3441-0.04660.230929.3261-41.6119-0.0579
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 2 through 69 )A2 - 69
2X-RAY DIFFRACTION2chain 'A' and (resid 70 through 95 )A70 - 95
3X-RAY DIFFRACTION3chain 'A' and (resid 96 through 142 )A96 - 142
4X-RAY DIFFRACTION4chain 'A' and (resid 143 through 177 )A143 - 177
5X-RAY DIFFRACTION5chain 'A' and (resid 178 through 245 )A178 - 245
6X-RAY DIFFRACTION6chain 'A' and (resid 246 through 265 )A246 - 265
7X-RAY DIFFRACTION7chain 'A' and (resid 266 through 277 )A266 - 277
8X-RAY DIFFRACTION8chain 'B' and (resid 2 through 18 )B2 - 18
9X-RAY DIFFRACTION9chain 'B' and (resid 19 through 42 )B19 - 42
10X-RAY DIFFRACTION10chain 'B' and (resid 43 through 58 )B43 - 58
11X-RAY DIFFRACTION11chain 'B' and (resid 59 through 80 )B59 - 80
12X-RAY DIFFRACTION12chain 'B' and (resid 81 through 95 )B81 - 95
13X-RAY DIFFRACTION13chain 'B' and (resid 96 through 115 )B96 - 115
14X-RAY DIFFRACTION14chain 'B' and (resid 116 through 154 )B116 - 154
15X-RAY DIFFRACTION15chain 'B' and (resid 155 through 177 )B155 - 177
16X-RAY DIFFRACTION16chain 'B' and (resid 178 through 229 )B178 - 229
17X-RAY DIFFRACTION17chain 'B' and (resid 230 through 245 )B230 - 245
18X-RAY DIFFRACTION18chain 'B' and (resid 246 through 259 )B246 - 259
19X-RAY DIFFRACTION19chain 'C' and (resid 3 through 164 )C3 - 164
20X-RAY DIFFRACTION20chain 'C' and (resid 165 through 189 )C165 - 189
21X-RAY DIFFRACTION21chain 'C' and (resid 190 through 259 )C190 - 259
22X-RAY DIFFRACTION22chain 'D' and (resid 3 through 189 )D3 - 189
23X-RAY DIFFRACTION23chain 'D' and (resid 190 through 259 )D190 - 259
24X-RAY DIFFRACTION24chain 'E' and (resid 2 through 189 )E2 - 189
25X-RAY DIFFRACTION25chain 'E' and (resid 190 through 279 )E190 - 279
26X-RAY DIFFRACTION26chain 'F' and (resid 2 through 58 )F2 - 58
27X-RAY DIFFRACTION27chain 'F' and (resid 59 through 81 )F59 - 81
28X-RAY DIFFRACTION28chain 'F' and (resid 82 through 115 )F82 - 115
29X-RAY DIFFRACTION29chain 'F' and (resid 116 through 154 )F116 - 154
30X-RAY DIFFRACTION30chain 'F' and (resid 155 through 177 )F155 - 177
31X-RAY DIFFRACTION31chain 'F' and (resid 178 through 225 )F178 - 225
32X-RAY DIFFRACTION32chain 'F' and (resid 226 through 260 )F226 - 260

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