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Yorodumi- PDB-5u1l: Crystal structure of the ATP-gated P2X7 ion channel in the closed... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5u1l | |||||||||
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Title | Crystal structure of the ATP-gated P2X7 ion channel in the closed, apo state | |||||||||
Components | P2X purinoceptor | |||||||||
Keywords | MEMBRANE PROTEIN / ATP-gated ion channel / no ligand / closed state | |||||||||
Function / homology | Function and homology information NAD transport / phagolysosome assembly / phospholipid transfer to membrane / purinergic nucleotide receptor activity / extracellularly ATP-gated monoatomic cation channel activity / gamma-aminobutyric acid secretion / positive regulation of interleukin-1 alpha production / negative regulation of cell volume / plasma membrane organization / collagen metabolic process ...NAD transport / phagolysosome assembly / phospholipid transfer to membrane / purinergic nucleotide receptor activity / extracellularly ATP-gated monoatomic cation channel activity / gamma-aminobutyric acid secretion / positive regulation of interleukin-1 alpha production / negative regulation of cell volume / plasma membrane organization / collagen metabolic process / positive regulation of gamma-aminobutyric acid secretion / pore complex assembly / : / positive regulation of prostaglandin secretion / response to fluid shear stress / T cell apoptotic process / ceramide biosynthetic process / bleb assembly / mitochondrial depolarization / vesicle budding from membrane / positive regulation of T cell apoptotic process / glutamate secretion / prostaglandin secretion / cellular response to dsRNA / positive regulation of glutamate secretion / skeletal system morphogenesis / negative regulation of bone resorption / positive regulation of macrophage cytokine production / phospholipid translocation / positive regulation of mitochondrial depolarization / response to ATP / response to zinc ion / negative regulation of MAPK cascade / T cell homeostasis / synaptic vesicle exocytosis / membrane protein ectodomain proteolysis / protein secretion / positive regulation of bone mineralization / T cell proliferation / response to mechanical stimulus / response to electrical stimulus / homeostasis of number of cells within a tissue / extrinsic apoptotic signaling pathway / release of sequestered calcium ion into cytosol / sensory perception of pain / reactive oxygen species metabolic process / mitochondrion organization / positive regulation of interleukin-1 beta production / positive regulation of protein secretion / lipopolysaccharide binding / protein catabolic process / neuromuscular junction / cell morphogenesis / T cell mediated cytotoxicity / protein processing / positive regulation of T cell mediated cytotoxicity / positive regulation of interleukin-6 production / response to calcium ion / MAPK cascade / cell-cell junction / presynapse / postsynapse / response to lipopolysaccharide / positive regulation of MAPK cascade / defense response to Gram-positive bacterium / response to xenobiotic stimulus / inflammatory response / positive regulation of protein phosphorylation / external side of plasma membrane / neuronal cell body / mitochondrion / ATP binding / identical protein binding Similarity search - Function | |||||||||
Biological species | Ailuropoda melanoleuca (giant panda) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.4 Å | |||||||||
Authors | Karasawa, A. / Kawate, T. | |||||||||
Funding support | United States, 2items
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Citation | Journal: Elife / Year: 2016 Title: Structural basis for subtype-specific inhibition of the P2X7 receptor. Authors: Karasawa, A. / Kawate, T. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5u1l.cif.gz | 76.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5u1l.ent.gz | 54.9 KB | Display | PDB format |
PDBx/mmJSON format | 5u1l.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/u1/5u1l ftp://data.pdbj.org/pub/pdb/validation_reports/u1/5u1l | HTTPS FTP |
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-Related structure data
Related structure data | 5u1uC 5u1vC 5u1wC 5u1xC 5u1yC 5u2hC C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Symmetry | Point symmetry: (Schoenflies symbol: I (icosahedral)) |
-Components
#1: Protein | Mass: 38716.234 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Electron density at the N- and C-termini is not well-defined Source: (gene. exp.) Ailuropoda melanoleuca (giant panda) / Gene: P2RX7 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: G1M6C4 |
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#2: Sugar |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 5.6 Å3/Da / Density % sol: 78.02 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 100 mM HEPES (pH 7.0), 100 mM NaCl, 4% ethylene glycol, 15% glycerol, 29% PEG-400, 0.1 mg/mL lipid mixture (60% POPE, 20% POPG, and 20% cholesterol). |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 1.1051 Å | ||||||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Aug 12, 2015 | ||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1.1051 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||
Reflection | Resolution: 3.4→48.8 Å / Num. obs: 11207 / % possible obs: 99.9 % / Redundancy: 9.8 % / Biso Wilson estimate: 130.9 Å2 / CC1/2: 1 / Rmerge(I) obs: 0.099 / Rpim(I) all: 0.033 / Rrim(I) all: 0.105 / Net I/σ(I): 16.9 / Num. measured all: 110336 | ||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1 / Rejects: 0
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: A740003 bound P2X7 Resolution: 3.4→45.183 Å / SU ML: 0.52 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 31.26
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 228.36 Å2 / Biso mean: 125.3547 Å2 / Biso min: 54.03 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3.4→45.183 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 8
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