Hippocalcin-likeprotein1 / Calcium-binding protein BDR-1 / HLP2 / Visinin-like protein 3 / VILIP-3
Mass: 22421.361 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Myristoyl group is covalently attached to the N-terminal Source: (gene. exp.) Homo sapiens (human) / Gene: HPCAL1, BDR1 / Production host: Escherichia coli (E. coli) / References: UniProt: P37235
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Sample state
Spectrometer-ID
Type
1
1
1
isotropic
1
3D 15N NOESY-HSQC
2
2
4
isotropic
1
3D 13C NOESY-HSQC
1
3
2
isotropic
1
3D 13C F1-filtered F3-edited HSQC-NOESY
1
4
3
isotropic
1
2D 1H-1H NOESY
1
7
1
isotropic
1
2D 1H-15N HSQC
1
6
2
isotropic
1
2D 1H-13C CT-HSQC
1
5
2
isotropic
1
3D HNCO
1
10
2
isotropic
1
3D HNCA
1
9
2
isotropic
1
3DCBCA(CO)NH
1
8
2
isotropic
1
3D HN(CA)CB
1
12
2
isotropic
1
3DHBHA(CO)NH
2
11
4
isotropic
1
3D (H)CCH-TOCSY
1
14
1
isotropic
1
3D TOCSY-HSQC
2
13
4
isotropic
1
3D (HB)CB(CGCD)HD
1
16
2
isotropic
1
3DHN(CA)CO
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Sample preparation
Details
Type
Solution-ID
Contents
Label
Solvent system
solution
1
0.5 mM [U-99% 15N] Myristoylated_VILIP-3, 5 mM deuterated Tris-d11, 4 mM deuterated DTT-d11, 0.3 mM deuterated EDTA-d12, 90% H2O/10% D2O
15N_sample
90% H2O/10% D2O
solution
2
0.5 mM [U-99% 15N],[U-99% 13C] Myristoylated_VILIP-3, 5 mM deuterated Tris-d11, 4 mM deuterated DTT-d11, 0.3 mM deuterated EDTA-d12, 90% H2O/10% D2O
13C,15N_sample
90% H2O/10% D2O
solution
4
0.5 mM [U-99% 15N],[U-99% 13C] Myristoylated_VILIP-3, 5 mM deuterated Tris-d11, 4 mM deuterated DTT-d11, 0.3 mM deuterated EDTA-d12, 100% D2O
13C,15N_sample-D2O
100% D2O
solution
3
0.5 mM Myristoylated_VILIP-3, 5 mM deuterated Tris-d11, 4 mM deuterated DTT-d11, 0.3 mM deuterated EDTA-d12, 100% D2O
unlabledsample
100% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
0.5mM
Myristoylated_VILIP-3
[U-99% 15N]
1
5mM
Tris-d11
deuterated
1
4mM
DTT-d11
deuterated
1
0.3mM
EDTA-d12
deuterated
1
0.5mM
Myristoylated_VILIP-3
[U-99% 15N],[U-99% 13C]
2
5mM
Tris-d11
deuterated
2
4mM
DTT-d11
deuterated
2
0.3mM
EDTA-d12
deuterated
2
0.5mM
Myristoylated_VILIP-3
[U-99% 15N],[U-99% 13C]
4
5mM
Tris-d11
deuterated
4
4mM
DTT-d11
deuterated
4
0.3mM
EDTA-d12
deuterated
4
0.5mM
Myristoylated_VILIP-3
naturalabundance
3
5mM
Tris-d11
deuterated
3
4mM
DTT-d11
deuterated
3
0.3mM
EDTA-d12
deuterated
3
Sample conditions
Conditions-ID
Ionic strength
Label
pH
Pressure (kPa)
Temperature (K)
1
6.4mM
condition-H2O
7.4
1atm
310K
2
6.4mM
Condition-D2O
7.4pH*
1atm
310K
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NMR measurement
NMR spectrometer
Type: Bruker AVANCE III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 800 MHz
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