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- PDB-5oka: Non-conservatively refined structure of Gan1D-E170Q, a catalytic ... -
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Basic information
Entry | Database: PDB / ID: 5oka | ||||||
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Title | Non-conservatively refined structure of Gan1D-E170Q, a catalytic mutant of a 6-phospho-beta-galactosidase from Geobacillus stearothermophilus | ||||||
![]() | Putative 6-phospho-beta-galactobiosidase | ||||||
![]() | HYDROLASE / 6-phospho-beta-galactosidase / Geobacillus stearothermophilus / Glycoside Hydrolase / GH1 family | ||||||
Function / homology | ![]() 6-phospho-beta-galactosidase / 6-phospho-beta-galactosidase activity / carbohydrate catabolic process / beta-glucosidase activity / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lansky, S. / Zehavi, A. / Shoham, Y. / Shoham, G. | ||||||
![]() | ![]() Title: Structural basis for enzyme bifunctionality - the case of Gan1D from Geobacillus stearothermophilus. Authors: Lansky, S. / Zehavi, A. / Belrhali, H. / Shoham, Y. / Shoham, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 870.4 KB | Display | ![]() |
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PDB format | ![]() | 724.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5ok7C ![]() 5okbC ![]() 5okeC ![]() 5okgC ![]() 5okhC ![]() 5okjC ![]() 5okkC ![]() 5okqC ![]() 5okrC ![]() 5oksC ![]() 4zeh C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 56208.730 Da / Num. of mol.: 4 / Mutation: E170Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: gan1D / Production host: ![]() ![]() #2: Chemical | ChemComp-GOL / #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.29 Å3/Da / Density % sol: 46.39 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 16-19% PEG 8K, 3% MPD, 0.1M imidazole buffer pH 6.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Apr 10, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 1.25→50 Å / Num. obs: 448160 / % possible obs: 80.2 % / Redundancy: 5.2 % / CC1/2: 0.999 / Rmerge(I) obs: 0.072 / Net I/σ(I): 11.9 |
Reflection shell | Resolution: 1.25→1.27 Å / Redundancy: 3 % / Rmerge(I) obs: 0.975 / Mean I/σ(I) obs: 1.1 / Num. unique obs: 5822 / CC1/2: 0.45 / % possible all: 21.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4ZEH ![]() 4zeh Resolution: 1.25→43.912 Å / SU ML: 0.11 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 16.42
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.25→43.912 Å
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Refine LS restraints |
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LS refinement shell |
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