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- PDB-5ok2: Structure of the D10N mutant of beta-phosphoglucomutase from Lact... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5ok2 | |||||||||
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Title | Structure of the D10N mutant of beta-phosphoglucomutase from Lactococcus lactis inhibited with glucose 6-phosphate and tetrafluoroaluminate to 1.1A resolution. | |||||||||
![]() | Beta-phosphoglucomutase | |||||||||
![]() | ISOMERASE / phosphoglucomutase / transition-state analogue / general acid-base catalysis | |||||||||
Function / homology | ![]() beta-phosphoglucomutase / beta-phosphoglucomutase activity / carbohydrate metabolic process / magnesium ion binding / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Robertson, A.J. / Bisson, C. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: van der Waals Contact between Nucleophile and Transferring Phosphorus Is Insufficient To Achieve Enzyme Transition-State Architecture Authors: Johnson, L.A. / Robertson, A.J. / Baxter, N.J. / Trevitt, C.R. / Bisson, C. / Jin, Y. / Wood, H.P. / Hounslow, A.M. / Cliff, M.J. / Blackburn, G.M. / Bowler, M.W. / Waltho, J.P. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 116.4 KB | Display | ![]() |
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PDB format | ![]() | 88.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 781 KB | Display | ![]() |
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Full document | ![]() | 780.8 KB | Display | |
Data in XML | ![]() | 12.9 KB | Display | |
Data in CIF | ![]() | 19.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5o6pC ![]() 5o6rC ![]() 5ojzC ![]() 5ok0C ![]() 5ok1C ![]() 2wf5S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein / Sugars , 2 types, 2 molecules A![](data/chem/img/BG6.gif)
![](data/chem/img/BG6.gif)
#1: Protein | Mass: 23852.119 Da / Num. of mol.: 1 / Mutation: K125R, Y206H, D10N Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: IL1403 / Gene: pgmB, LL0429, L0001 / Production host: ![]() ![]() |
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#6: Sugar | ChemComp-BG6 / |
-Non-polymers , 5 types, 251 molecules ![](data/chem/img/ALF.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-ALF / | ||||||
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#3: Chemical | ChemComp-EDO / #4: Chemical | ChemComp-NA / | #5: Chemical | #7: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 44.98 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7.2 Details: 24-34% PEG 4000 200mM Tris pH 7.2 200mM sodium acetate PH range: 7.2 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Apr 28, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97625 Å / Relative weight: 1 |
Reflection | Resolution: 1.1→54.28 Å / Num. obs: 87229 / % possible obs: 99.5 % / Redundancy: 6.8 % / CC1/2: 0.999 / Rmerge(I) obs: 0.07 / Rpim(I) all: 0.029 / Net I/σ(I): 12.5 |
Reflection shell | Resolution: 1.1→1.12 Å / Redundancy: 4.7 % / Rmerge(I) obs: 1.147 / Mean I/σ(I) obs: 1.2 / Num. unique obs: 3941 / CC1/2: 0.484 / Rpim(I) all: 0.572 / % possible all: 91.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2WF5 Resolution: 1.1→52.39 Å / Cor.coef. Fo:Fc: 0.978 / Cor.coef. Fo:Fc free: 0.971 / SU B: 1.04 / SU ML: 0.021 / Cross valid method: THROUGHOUT / ESU R: 0.03 / ESU R Free: 0.03 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 14.709 Å2
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Refinement step | Cycle: 1 / Resolution: 1.1→52.39 Å
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Refine LS restraints |
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