+Open data
-Basic information
Entry | Database: PDB / ID: 5oix | |||||||||||||||
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Title | Structure of the HMPV P oligomerization domain at 1.6 A | |||||||||||||||
Components | Phosphoprotein | |||||||||||||||
Keywords | VIRAL PROTEIN / Phosphoprotein / P protein / Mononegavirales / Viral replication / Tetramerization | |||||||||||||||
Function / homology | Phosphoprotein, pneumoviral / Pneumovirus phosphoprotein / RNA-dependent RNA polymerase activity / Phosphoprotein Function and homology information | |||||||||||||||
Biological species | Human metapneumovirus | |||||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.61 Å | |||||||||||||||
Authors | Renner, M. / Paesen, G.C. / Grison, C.M. / Granier, S. / Grimes, J.M. / Leyrat, C. | |||||||||||||||
Funding support | United Kingdom, France, 4items
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Citation | Journal: Sci Rep / Year: 2017 Title: Structural dissection of human metapneumovirus phosphoprotein using small angle x-ray scattering. Authors: Renner, M. / Paesen, G.C. / Grison, C.M. / Granier, S. / Grimes, J.M. / Leyrat, C. | |||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5oix.cif.gz | 113.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5oix.ent.gz | 84.9 KB | Display | PDB format |
PDBx/mmJSON format | 5oix.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 5oix_validation.pdf.gz | 489.2 KB | Display | wwPDB validaton report |
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Full document | 5oix_full_validation.pdf.gz | 489.8 KB | Display | |
Data in XML | 5oix_validation.xml.gz | 12.3 KB | Display | |
Data in CIF | 5oix_validation.cif.gz | 17.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/oi/5oix ftp://data.pdbj.org/pub/pdb/validation_reports/oi/5oix | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 13257.938 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Human metapneumovirus / Plasmid: pOPINF / Production host: Escherichia coli (E. coli) / Variant (production host): Rosetta2 / References: UniProt: Q91KZ5 #2: Chemical | ChemComp-GOL / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, sitting drop / pH: 8 Details: 20% polyethylene glycol (PEG) 6000, 200 mM NaCl, and 100 mM Tris, pH 8.0 |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.9795 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Apr 22, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 1.61→36.39 Å / Num. obs: 31091 / % possible obs: 99.32 % / Redundancy: 3.3 % / CC1/2: 0.999 / Rmerge(I) obs: 0.04575 / Rpim(I) all: 0.02941 / Net I/σ(I): 13.89 |
-Processing
Software |
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Refinement | Resolution: 1.61→36.39 Å / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 1.61→36.39 Å
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