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- PDB-5odp: Salinibacter ruber Single-Strand Binding protein D17K D71K mutant -
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Open data
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Basic information
Entry | Database: PDB / ID: 5odp | ||||||
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Title | Salinibacter ruber Single-Strand Binding protein D17K D71K mutant | ||||||
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![]() | DNA BINDING PROTEIN / Single-Strand Binding protein | ||||||
Function / homology | ![]() single-stranded DNA binding / DNA recombination / DNA replication / DNA repair Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Pierechod, M. / Rothweiler, U. | ||||||
![]() | ![]() Title: Salinibacter ruber Single-Strand Binding protein D17K D71K mutant Authors: Pierechod, M. / Rothweiler, U. #1: ![]() Title: Single-strand binding protein Authors: Pierechod, M. / Willassen, N.P. / Rothweiler, U. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 63.7 KB | Display | ![]() |
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PDB format | ![]() | 43.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 478.6 KB | Display | ![]() |
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Full document | ![]() | 480.1 KB | Display | |
Data in XML | ![]() | 9.4 KB | Display | |
Data in CIF | ![]() | 11.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5odnS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 21929.623 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: D17K and D71K point mutations Source: (gene. exp.) ![]() ![]() Gene: ssb, SRU_0523 / Production host: ![]() ![]() #2: DNA chain | Mass: 2996.971 Da / Num. of mol.: 5 / Source method: obtained synthetically / Details: 75 synthetic poly T single stranded DNA / Source: (synth.) ![]() ![]() #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: PEG 10k 9.9% |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jan 24, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.918 Å / Relative weight: 1 |
Reflection | Resolution: 2.535→50 Å / Num. obs: 11326 / % possible obs: 99.6 % / Redundancy: 12.7 % / CC1/2: 0.999 / Rrim(I) all: 0.122 / Net I/σ(I): 17.59 |
Reflection shell | Resolution: 2.535→2.69 Å / Mean I/σ(I) obs: 2.19 / Num. unique obs: 1764 / CC1/2: 0.678 / Rrim(I) all: 1.244 / % possible all: 98.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5ODN Resolution: 2.535→46.911 Å / SU ML: 0.33 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 25.47
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.535→46.911 Å
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Refine LS restraints |
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LS refinement shell |
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