- PDB-5o8b: Difference-refined excited-state structure of rsEGFP2 1ps followi... -
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Entry
Database: PDB / ID: 5o8b
Title
Difference-refined excited-state structure of rsEGFP2 1ps following 400nm-laser irradiation of the off-state.
Components
Green fluorescent protein
Keywords
FLUORESCENT PROTEIN / photoswitching on state fluorescent state chromophore SFX serial femtosecond crystallography CXI LCLS time resolved crystallography chromohore isomerisation
Green Fluorescent Protein / Green fluorescent protein / Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / Beta Barrel / Mainly Beta Similarity search - Domain/homology
Mass: 28099.686 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: HIS A -8 EXPRESSION TAG HIS A -7 EXPRESSION TAG HIS A -6 EXPRESSION TAG HIS A -5 EXPRESSION TAG HIS A -4 EXPRESSION TAG HIS A -3 EXPRESSION TAG THR A -2 EXPRESSION TAG ASP A -1 EXPRESSION ...Details: HIS A -8 EXPRESSION TAG HIS A -7 EXPRESSION TAG HIS A -6 EXPRESSION TAG HIS A -5 EXPRESSION TAG HIS A -4 EXPRESSION TAG HIS A -3 EXPRESSION TAG THR A -2 EXPRESSION TAG ASP A -1 EXPRESSION TAG PRO A 0 EXPRESSION TAG MET A 1 EXPRESSION TAG VAL A 2 MET 1 ENGINEERED MUTATION LEU A 65 PHE 64 ENGINEERED MUTATION PIA A 68 SER 65 CHROMOPHORE PIA A 68 TYR 66 CHROMOPHORE PIA A 68 GLY 67 CHROMOPHORE LEU A 70 GLN 69 ENGINEERED MUTATION SER A 164 VAL 163 ENGINEERED MUTATION LYS A 207 ALA 206 ENGINEERED MUTATION LEU A 232 HIS 231 ENGINEERED MUTATION Source: (gene. exp.) Aequorea victoria (jellyfish) / Gene: GFP / Production host: Escherichia coli (E. coli) / References: UniProt: P42212
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