- PDB-5nyz: Twist and induce: Dissecting the link between the enzymatic activ... -
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ID or keywords:
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Basic information
Entry
Database: PDB / ID: 5nyz
Title
Twist and induce: Dissecting the link between the enzymatic activity and the SaPI inducing capacity of the phage 80 dUTPase. D95E mutant from dUTPase 80alpha phage.
Components
DUTPase
Keywords
HYDROLASE / dUTPase / 80 phage / S.aureus
Function / homology
Function and homology information
dUTP catabolic process / dUMP biosynthetic process / dUTP diphosphatase / dUTP diphosphatase activity / magnesium ion binding Similarity search - Function
Mass: 19128.695 Da / Num. of mol.: 1 / Mutation: D95E Source method: isolated from a genetically manipulated source Details: Ordered C-terminal is placed over the nucleotide on active center. Magnesium atom is coordinated by E95 residue. Source: (gene. exp.) Staphylococcus phage 80alpha (virus) / Plasmid: pet28a / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A4ZF98
Resolution: 2.5→61.71 Å / Cor.coef. Fo:Fc: 0.938 / Cor.coef. Fo:Fc free: 0.894 / SU B: 11.95 / SU ML: 0.265 / Cross valid method: THROUGHOUT / ESU R: 0.452 / ESU R Free: 0.301 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.28055
374
4.7 %
RANDOM
Rwork
0.22569
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obs
0.22853
7533
100 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å