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Yorodumi- PDB-5n9u: Dehydroascorbate reductase 3A from Populus trichocarpa complexed ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5n9u | ||||||
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Title | Dehydroascorbate reductase 3A from Populus trichocarpa complexed with GSH. | ||||||
Components | Dehydroascorbate reductase family protein | ||||||
Keywords | OXIDOREDUCTASE / Glutathione transferase / Dehydroascorbate reductase / Plant | ||||||
Function / homology | Function and homology information ascorbate glutathione cycle / glutathione dehydrogenase (ascorbate) / glutathione dehydrogenase (ascorbate) activity / glutathione transferase activity Similarity search - Function | ||||||
Biological species | Populus trichocarpa (black cottonwood) | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
Authors | Roret, T. / Tsan, P. | ||||||
Citation | Journal: Biochem.J. / Year: 2016 Title: Insights into ascorbate regeneration in plants: investigating the redox and structural properties of dehydroascorbate reductases from Populus trichocarpa. Authors: Lallement, P.A. / Roret, T. / Tsan, P. / Gualberto, J.M. / Girardet, J.M. / Didierjean, C. / Rouhier, N. / Hecker, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5n9u.cif.gz | 668.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5n9u.ent.gz | 580.1 KB | Display | PDB format |
PDBx/mmJSON format | 5n9u.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n9/5n9u ftp://data.pdbj.org/pub/pdb/validation_reports/n9/5n9u | HTTPS FTP |
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-Related structure data
Related structure data | 2n5fC 5myeC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 24364.195 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Populus trichocarpa (black cottonwood) / Gene: POPTR_0008s04920g / Production host: Escherichia coli (E. coli) / References: UniProt: B9HM36 |
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#2: Chemical | ChemComp-GSH / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Sample state: isotropic / Type: 2D 1H-15N HSQC |
-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 50 mM / Label: condition_1 / pH: 8 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: Avance / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: target function | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 |