- PDB-5n8n: Contracted sheath of a Pseudomonas aeruginosa type six secretion ... -
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基本情報
登録情報
データベース: PDB / ID: 5n8n
タイトル
Contracted sheath of a Pseudomonas aeruginosa type six secretion system consisting of TssB1 and TssC1
要素
EvpB family type VI secretion protein
Type VI secretion protein, family
キーワード
STRUCTURAL PROTEIN / type six secretion system
機能・相同性
機能・相同性情報
Type VI secretion system TssC-like / TssC1, N-terminal / TssC1, C-terminal / EvpB/VC_A0108, tail sheath N-terminal domain / EvpB/VC_A0108, tail sheath gpW/gp25-like domain / Type VI secretion system sheath protein TssB1 / Type VI secretion system, VipA, VC_A0107 or Hcp2 類似検索 - ドメイン・相同性
Type VI secretion protein / : / Type VI secretion system sheath protein TssC1 / Type VI secretion system sheath protein TssB1 類似検索 - 構成要素
ジャーナル: Structure / 年: 2018 タイトル: Atomic Structure of Type VI Contractile Sheath from Pseudomonas aeruginosa. 著者: Osman Salih / Shaoda He / Sara Planamente / Lasse Stach / James T MacDonald / Eleni Manoli / Sjors H W Scheres / Alain Filloux / Paul S Freemont / 要旨: Pseudomonas aeruginosa has three type VI secretion systems (T6SSs), H1-, H2-, and H3-T6SS, each belonging to a distinct group. The two T6SS components, TssB/VipA and TssC/VipB, assemble to form ...Pseudomonas aeruginosa has three type VI secretion systems (T6SSs), H1-, H2-, and H3-T6SS, each belonging to a distinct group. The two T6SS components, TssB/VipA and TssC/VipB, assemble to form tubules that conserve structural/functional homology with tail sheaths of contractile bacteriophages and pyocins. Here, we used cryoelectron microscopy to solve the structure of the H1-T6SS P. aeruginosa TssB1C1 sheath at 3.3 Å resolution. Our structure allowed us to resolve some features of the T6SS sheath that were not resolved in the Vibrio cholerae VipAB and Francisella tularensis IglAB structures. Comparison with sheath structures from other contractile machines, including T4 phage and R-type pyocins, provides a better understanding of how these systems have conserved similar functions/mechanisms despite evolution. We used the P. aeruginosa R2 pyocin as a structural template to build an atomic model of the TssB1C1 sheath in its extended conformation, allowing us to propose a coiled-spring-like mechanism for T6SS sheath contraction.
A: Type VI secretion protein, family B: EvpB family type VI secretion protein C: Type VI secretion protein, family D: EvpB family type VI secretion protein E: Type VI secretion protein, family F: EvpB family type VI secretion protein G: Type VI secretion protein, family H: EvpB family type VI secretion protein I: Type VI secretion protein, family J: EvpB family type VI secretion protein K: Type VI secretion protein, family L: EvpB family type VI secretion protein M: Type VI secretion protein, family N: EvpB family type VI secretion protein O: Type VI secretion protein, family P: EvpB family type VI secretion protein Q: Type VI secretion protein, family R: EvpB family type VI secretion protein S: Type VI secretion protein, family T: EvpB family type VI secretion protein U: Type VI secretion protein, family V: EvpB family type VI secretion protein W: Type VI secretion protein, family X: EvpB family type VI secretion protein Y: Type VI secretion protein, family Z: EvpB family type VI secretion protein a: Type VI secretion protein, family b: EvpB family type VI secretion protein c: Type VI secretion protein, family d: EvpB family type VI secretion protein