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- PDB-5mva: Structure of the thin filament at high calcium concentration -

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Basic information

Entry
Database: PDB / ID: 5mva
TitleStructure of the thin filament at high calcium concentration
DescriptorActin, alpha skeletal muscle
KeywordsSTRUCTURAL PROTEIN / Thin filament / troponin / actin / tropomyosin / Structural protein
Specimen sourceOryctolagus cuniculus / mammal / Rabbit / アナウサギ /
MethodElectron microscopy (27.7 Å resolution / Filament / Single particle)
AuthorsPaul, D.M. / Squire, J.M. / Morris, E.P.
CitationJ. Struct. Biol., 2017, 197, 365-371

J. Struct. Biol., 2017, 197, 365-371 Yorodumi Papers
Relaxed and active thin filament structures; a new structural basis for the regulatory mechanism.
Danielle M Paul / John M Squire / Edward P Morris

Validation Report
SummaryFull reportAbout validation report
DateDeposition: Jan 16, 2017 / Release: Nov 29, 2017

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Assembly

Deposited unit
A: Actin, alpha skeletal muscle
B: Actin, alpha skeletal muscle
C: Actin, alpha skeletal muscle
D: Actin, alpha skeletal muscle
E: Actin, alpha skeletal muscle
F: Actin, alpha skeletal muscle
G: Actin, alpha skeletal muscle
H: Actin, alpha skeletal muscle
I: Actin, alpha skeletal muscle
J: Actin, alpha skeletal muscle
K: Actin, alpha skeletal muscle
L: Actin, alpha skeletal muscle
M: Actin, alpha skeletal muscle
N: Actin, alpha skeletal muscle
O: Actin, alpha skeletal muscle
P: Actin, alpha skeletal muscle
Q: Actin, alpha skeletal muscle
R: Actin, alpha skeletal muscle
S: Actin, alpha skeletal muscle
T: Actin, alpha skeletal muscle
U: Actin, alpha skeletal muscle
V: Actin, alpha skeletal muscle
W: Actin, alpha skeletal muscle
hetero molecules


Theoretical massNumber of molelcules
Total (without water)972,96546
Polyers963,14023
Non-polymers9,82623
Water0
#1


TypeNameSymmetry operationNumber
identity operation1_5551
Buried area (Å2)69640
ΔGint (kcal/M)-425
Surface area (Å2)371690
MethodPISA

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Components

#1: Polypeptide(L) ...
Actin, alpha skeletal muscle / Alpha-actin-1


Mass: 41875.633 Da / Num. of mol.: 23
Source: (natural) Oryctolagus cuniculus / mammal / アナウサギ /
References: UniProt: P68135
#2: Chemical...
ChemComp-ADP / ADENOSINE-5'-DIPHOSPHATE / Alpha-actin-1 / ADP *YM


Mass: 427.201 Da / Num. of mol.: 23 / Formula: C10H15N5O10P2

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: FILAMENT / Reconstruction method: SINGLE PARTICLE

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Sample preparation

ComponentName: Thin filament at high calcium concentration / Type: COMPLEX / Entity ID: 1 / Source: NATURAL
Source (natural)Organism: Oryctolagus cuniculus
Buffer solutionpH: 7
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: YES / Vitrification applied: NO
EM stainingType: NEGATIVE / Material: Uranyl Acetate

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Electron microscopy imaging

MicroscopyMicroscope model: FEI/PHILIPS CM12
Electron gunElectron source: LAB6 / Accelerating voltage: 120 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD
Image recordingElectron dose: 12 e/Å2 / Film or detector model: AGFA SCIENTA FILM
Image scansScanner model: ZEISS SCAI

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Processing

CTF correctionType: NONE
3D reconstructionResolution: 27.7 Å / Resolution method: FSC 0.5 CUT-OFF / Number of particles: 1680 / Symmetry type: POINT

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