A: Induced myeloid leukemia cell differentiation protein Mcl-1 homolog,Induced myeloid leukemia cell differentiation protein Mcl-1 H: Fab Heavy Chain L: Fab Light Chain hetero molecules
InducedmyeloidleukemiacelldifferentiationproteinMcl-1homolog,InducedmyeloidleukemiacelldifferentiationproteinMcl-1 / Bcl-2-related protein EAT/mcl1 / Bcl-2-like protein 3 / Bcl2-L-3 / Bcl-2-related protein EAT/mcl1 / mcl1/EAT
Mass: 18227.592 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse), (gene. exp.) Homo sapiens (human) Gene: Mcl1, MCL1, BCL2L3 / Production host: Escherichia coli (E. coli) / References: UniProt: P97287, UniProt: Q07820
#2: Antibody
FabHeavyChain
Mass: 24488.490 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli)
#3: Antibody
FabLightChain
Mass: 24157.469 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli)
Resolution: 2.94→3.01 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.637 / Mean I/σ(I) obs: 1.1 / CC1/2: 0.375 / % possible all: 89
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Processing
Software
Name
Version
Classification
REFMAC
5.8.0135
refinement
MOSFLM
datareduction
SCALA
datascaling
PHASER
phasing
Refinement
Resolution: 2.94→26.45 Å / Cor.coef. Fo:Fc: 0.921 / Cor.coef. Fo:Fc free: 0.864 / SU B: 23.329 / SU ML: 0.424 / Cross valid method: THROUGHOUT / ESU R Free: 0.572 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.30248
597
5 %
RANDOM
Rwork
0.22502
-
-
-
obs
0.229
11314
98.63 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK