PDB-5mei: Crystal structure of Agelastatin A bound to the 80S ribosome

Basic information

• Entry: Database: PDB / ID: 5mei
• Title: Crystal structure of Agelastatin A bound to the 80S ribosome

Components

• (40S ribosomal protein ...) x 32
• (60S ribosomal protein ...) x 40
• (Suppressor protein ...) x 2
• (Ubiquitin-40S ribosomal protein ...) x 2
• 18S ribosomal RNA
• 25S ribosomal RNA
• 5.8S ribosomal RNA
• 5S ribosomal RNA
• 60S acidic ribosomal protein P0
• Guanine nucleotide-binding protein subunit beta-like protein
• Ubiquitin-60S ribosomal protein L40

• Keywords: RIBOSOME / 80S ribosome / protein synthesis inhibitor

Function / homology

Function:

triplex DNA binding / ribosome hibernation / translation elongation factor binding / Platelet degranulation / regulation of translational initiation in response to stress / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / Negative regulators of DDX58/IFIH1 signaling / regulation of amino acid metabolic process / negative regulation of glucose mediated signaling pathway / positive regulation of translational fidelity / RMTs methylate histone arginines / Protein methylation / mTORC1-mediated signalling / Protein hydroxylation / ribosome-associated ubiquitin-dependent protein catabolic process / GDP-dissociation inhibitor activity / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / pre-mRNA 5'-splice site binding / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / preribosome, small subunit precursor / nonfunctional rRNA decay / response to cycloheximide / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / telomeric DNA binding / Major pathway of rRNA processing in the nucleolus and cytosol / mRNA destabilization / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / negative regulation of translational frameshifting / TOR signaling / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / Formation of a pool of free 40S subunits / preribosome, large subunit precursor / positive regulation of protein kinase activity / L13a-mediated translational silencing of Ceruloplasmin expression / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit export from nucleus / translational elongation / G-protein alpha-subunit binding / 90S preribosome / Ub-specific processing proteases / ribosomal subunit export from nucleus / regulation of translational fidelity / protein-RNA complex assembly / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translational termination / maturation of LSU-rRNA / ribosomal small subunit export from nucleus / translation regulator activity / translation repressor activity / DNA-(apurinic or apyrimidinic site) endonuclease activity / rescue of stalled ribosome / telomere maintenance / cellular response to amino acid starvation / protein kinase C binding / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosome assembly / maturation of SSU-rRNA / small-subunit processome / translational initiation / macroautophagy / maintenance of translational fidelity / modification-dependent protein catabolic process / protein tag activity / cytoplasmic stress granule / rRNA processing / ribosome biogenesis / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / small ribosomal subunit rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / protein ubiquitination / structural constituent of ribosome / ribosome / translation / G protein-coupled receptor signaling pathway / negative regulation of gene expression / response to antibiotic / mRNA binding / ubiquitin protein ligase binding / negative regulation of apoptotic process / nucleolus / perinuclear region of cytoplasm / mitochondrion / DNA binding

Domain/homology:

Stm1-like, N-terminal / Stm1 / Hyaluronan/mRNA-binding protein / Hyaluronan / mRNA binding family / 60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / : / 50S ribosomal protein L10, insertion domain superfamily / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / : / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / : / Ribosomal protein S12e signature. / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S12e / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein S5, eukaryotic/archaeal / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / S27a-like superfamily / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / 40S Ribosomal protein S10 / : / Ribosomal protein S7e signature. / Ribosomal protein L19, eukaryotic / Ribosomal protein L10e / Ribosomal protein L24e, conserved site / : / Ribosomal protein L24e signature. / Ribosomal protein L44e signature. / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L6e signature. / Ribosomal protein L13e / Ribosomal protein L13e / Plectin/S10, N-terminal / Plectin/S10 domain / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S30 / Ribosomal protein S17e, conserved site / Ribosomal protein S30 / Ribosomal protein S17e signature. / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein S3Ae, conserved site / Ribosomal protein S3Ae signature. / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / : / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L30e signature 1. / Ribosomal L40e family / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein 60S L18 and 50S L18e / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein L36e signature. / Ribosomal protein L35Ae, conserved site / : / Ribosomal protein L35Ae signature. / Ribosomal_L40e

Component:

Agelastatin A / osmium (III) hexammine / : / : / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / : / Small ribosomal subunit protein uS4A / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein eS17A / Large ribosomal subunit protein eL24A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL15A / Large ribosomal subunit protein eL22A / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS15 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein eS19A / Small ribosomal subunit protein eS21A / Small ribosomal subunit protein uS8A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL31A / Ubiquitin-ribosomal protein eL40A fusion protein / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL42A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS30A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Small ribosomal subunit protein eS8A / Large ribosomal subunit protein uL14A / Large ribosomal subunit protein uL2A / Small ribosomal subunit protein uS17A / Large ribosomal subunit protein eL18A / Small ribosomal subunit protein uS9A / Small ribosomal subunit protein uS13A / Large ribosomal subunit protein eL19A / Large ribosomal subunit protein uL29A / Small ribosomal subunit protein eS32A / Small ribosomal subunit protein eS32B / Large ribosomal subunit protein uL4A / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein eS17B / Large ribosomal subunit protein eL8A / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL13A / Small ribosomal subunit protein eS7A / Small ribosomal subunit protein uS2A / Small ribosomal subunit protein eS1A / Small ribosomal subunit protein eS27A / Large ribosomal subunit protein eL14A / Small ribosomal subunit protein RACK1 / Large ribosomal subunit protein eL32 / Small ribosomal subunit protein uS10 / Suppressor protein STM1 / Small ribosomal subunit protein uS11B / Small ribosomal subunit protein eS26B / Small ribosomal subunit protein uS14A / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein eS12 / Large ribosomal subunit protein eL37A / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein eL34A / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein eL6A / Large ribosomal subunit protein eL21A / Small ribosomal subunit protein eS10A / Large ribosomal subunit protein eL13A / Large ribosomal subunit protein uL5B / Small ribosomal subunit protein eS25A / Small ribosomal subunit protein eS28A

• Biological species: Saccharomyces cerevisiae (brewer's yeast)
• Method: X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.5 Å
• Authors: McClary, B. / Zinshteyn, B. / Meyer, M. / Jouanneau, M. / Pellegrino, S. / Yusupova, G. / Schuller, A. / Reyes, J.C.P. / Lu, J. / Luo, C. / Dang, Y. / Romo, D. / Yusupov, M. / Green, R. / Liu, J.O.

Funding support

United States, France, 2items

Organization	Grant number	Country
National Institutes of Health	R37 GM052964	United States
European Research Council	294312	France

• Citation: Journal: Cell Chem Biol / Year: 2017; Title: Inhibition of Eukaryotic Translation by the Antitumor Natural Product Agelastatin A.; Authors: McClary, B. / Zinshteyn, B. / Meyer, M. / Jouanneau, M. / Pellegrino, S. / Yusupova, G. / Schuller, A. / Reyes, J.C.P. / Lu, J. / Guo, Z. / Ayinde, S. / Luo, C. / Dang, Y. / Romo, D. / Yusupov, M. / Green, R. / Liu, J.O.

History

Deposition	Nov 15, 2016	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Jun 28, 2017	Provider: repository / Type: Initial release
Revision 1.1	Jul 5, 2017	Group: Structure summary / Category: struct / Item: _struct.title
Revision 1.2	Jan 31, 2018	Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.3	Jan 17, 2024	Group: Data collection / Database references / Derived calculations / Refinement description Category: chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id

Assembly

Deposited unit

1: 25S ribosomal RNA

3: 5S ribosomal RNA

4: 5.8S ribosomal RNA

j: 60S ribosomal protein L2-A

k: 60S ribosomal protein L3

l: 60S ribosomal protein L4-A

m: 60S ribosomal protein L5

n: 60S ribosomal protein L6-A

o: 60S ribosomal protein L7-A

p: 60S ribosomal protein L8-A

q: 60S ribosomal protein L9-A

r: 60S ribosomal protein L10

s: 60S ribosomal protein L11-B

t: 60S ribosomal protein L13-A

u: 60S ribosomal protein L14-A

v: 60S ribosomal protein L15-A

w: 60S ribosomal protein L16-A

x: 60S ribosomal protein L17-A

y: 60S ribosomal protein L18-A

z: 60S ribosomal protein L19-A

0: 60S ribosomal protein L20-A

2: 60S ribosomal protein L21-A

5: 60S ribosomal protein L22-A

l2: 60S ribosomal protein L23-A

6: 18S ribosomal RNA

7: 60S ribosomal protein L24-A

8: 60S ribosomal protein L25

9: 60S ribosomal protein L26-A

AA: 60S ribosomal protein L27-A

AB: 60S ribosomal protein L28

AC: 60S ribosomal protein L29

AD: 60S ribosomal protein L30

AE: 60S ribosomal protein L31-A

AF: 60S ribosomal protein L32

AG: 60S ribosomal protein L33-A

AH: 60S ribosomal protein L34-A

AI: 60S ribosomal protein L35-A

AJ: 60S ribosomal protein L36-A

AK: 60S ribosomal protein L37-A

AL: 60S ribosomal protein L38

AM: 60S ribosomal protein L39

AN: Ubiquitin-60S ribosomal protein L40

AO: 60S ribosomal protein L41-B

AP: 60S ribosomal protein L42-A

AQ: 60S ribosomal protein L43-A

i: Suppressor protein STM1

AR: 25S ribosomal RNA

AS: 5S ribosomal RNA

AT: 5.8S ribosomal RNA

CD: 60S ribosomal protein L2-A

CE: 60S ribosomal protein L3

CF: 60S ribosomal protein L4-A

CG: 60S ribosomal protein L5

CH: 60S ribosomal protein L6-A

CI: 60S ribosomal protein L7-A

CJ: 60S ribosomal protein L8-A

CK: 60S ribosomal protein L9-A

CL: 60S ribosomal protein L10

CM: 60S ribosomal protein L11-B

CN: 60S ribosomal protein L13-A

CO: 60S ribosomal protein L14-A

CP: 60S ribosomal protein L15-A

CQ: 60S ribosomal protein L16-A

CR: 60S ribosomal protein L17-A

CS: 60S ribosomal protein L18-A

CT: 60S ribosomal protein L19-A

CU: 60S ribosomal protein L20-A

CV: 60S ribosomal protein L21-A

CW: 60S ribosomal protein L22-A

CX: 60S ribosomal protein L23-A

CY: 60S ribosomal protein L24-A

CZ: 60S ribosomal protein L25

DA: 60S ribosomal protein L26-A

DB: 60S ribosomal protein L27-A

DC: 60S ribosomal protein L28

DD: 60S ribosomal protein L29

DE: 60S ribosomal protein L30

DF: 60S ribosomal protein L31-A

DG: 60S ribosomal protein L32

DH: 60S ribosomal protein L33-A

DI: 60S ribosomal protein L34-A

DJ: 60S ribosomal protein L35-A

DK: 60S ribosomal protein L36-A

DL: 60S ribosomal protein L37-A

DM: 60S ribosomal protein L38

DN: 60S ribosomal protein L39

DO: Ubiquitin-60S ribosomal protein L40

DP: 60S ribosomal protein L41-B

DQ: 60S ribosomal protein L42-A

DR: 60S ribosomal protein L43-A

p0: 60S acidic ribosomal protein P0

sM: Suppressor protein STM1,Suppressor protein STM1,Suppressor protein Stm1 - Mol B

A: 18S ribosomal RNA

B: 40S ribosomal protein S0-A

C: 40S ribosomal protein S1-A

D: 40S ribosomal protein S2

E: 40S ribosomal protein S3

F: 40S ribosomal protein S4-A

G: 40S ribosomal protein S5

H: 40S ribosomal protein S6-A

I: 40S ribosomal protein S7-A

J: 40S ribosomal protein S8-A

K: 40S ribosomal protein S9-A

L: 40S ribosomal protein S10-A

M: 40S ribosomal protein S11-A

N: 40S ribosomal protein S12

O: 40S ribosomal protein S13

P: 40S ribosomal protein S14-B

Q: 40S ribosomal protein S15

R: 40S ribosomal protein S16-A

S: 40S ribosomal protein S17-B

T: 40S ribosomal protein S18-A

U: 40S ribosomal protein S19-A

V: 40S ribosomal protein S20

W: 40S ribosomal protein S21-A

X: 40S ribosomal protein S22-A

Y: 40S ribosomal protein S23-A

Z: 40S ribosomal protein S24-A

a: 40S ribosomal protein S25-A

b: 40S ribosomal protein S26-B

c: 40S ribosomal protein S27-A

d: 40S ribosomal protein S28-A

e: 40S ribosomal protein S29-A

f: 40S ribosomal protein S30-A

g: Ubiquitin-40S ribosomal protein S31

h: Guanine nucleotide-binding protein subunit beta-like protein

s0: 40S ribosomal protein S0-A

s1: 40S ribosomal protein S1-A

s2: 40S ribosomal protein S2

s3: 40S ribosomal protein S3

s4: 40S ribosomal protein S4-A

s5: 40S ribosomal protein S5

s6: 40S ribosomal protein S6-A

s7: 40S ribosomal protein S7-A

s8: 40S ribosomal protein S8-A

s9: 40S ribosomal protein S9-A

c0: 40S ribosomal protein S10-A

c1: 40S ribosomal protein S11-A

c2: 40S ribosomal protein S12

c3: 40S ribosomal protein S13

c4: 40S ribosomal protein S14-B

c5: 40S ribosomal protein S15

c6: 40S ribosomal protein S16-A

c7: 40S ribosomal protein S17-A

c8: 40S ribosomal protein S18-A

c9: 40S ribosomal protein S19-A

d0: 40S ribosomal protein S20

d1: 40S ribosomal protein S21-A

d2: 40S ribosomal protein S22-A

d3: 40S ribosomal protein S23-A

d4: 40S ribosomal protein S24-A

d5: 40S ribosomal protein S25-A

d6: 40S ribosomal protein S26-B

d7: 40S ribosomal protein S27-A

d8: 40S ribosomal protein S28-A

d9: 40S ribosomal protein S29-A

e0: 40S ribosomal protein S30-A

e1: Ubiquitin-40S ribosomal protein S31

sR: Guanine nucleotide-binding protein subunit beta-like protein

hetero molecules

Summary:

• 6.45 MDa, 159 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	6,450,160	2653
Polymers	6,107,848	159
Non-polymers	342,312	2494
Water	0	0

1

1: 25S ribosomal RNA

3: 5S ribosomal RNA

4: 5.8S ribosomal RNA

j: 60S ribosomal protein L2-A

k: 60S ribosomal protein L3

l: 60S ribosomal protein L4-A

m: 60S ribosomal protein L5

n: 60S ribosomal protein L6-A

o: 60S ribosomal protein L7-A

p: 60S ribosomal protein L8-A

q: 60S ribosomal protein L9-A

r: 60S ribosomal protein L10

s: 60S ribosomal protein L11-B

t: 60S ribosomal protein L13-A

u: 60S ribosomal protein L14-A

v: 60S ribosomal protein L15-A

w: 60S ribosomal protein L16-A

x: 60S ribosomal protein L17-A

y: 60S ribosomal protein L18-A

z: 60S ribosomal protein L19-A

0: 60S ribosomal protein L20-A

2: 60S ribosomal protein L21-A

5: 60S ribosomal protein L22-A

l2: 60S ribosomal protein L23-A

7: 60S ribosomal protein L24-A

8: 60S ribosomal protein L25

9: 60S ribosomal protein L26-A

AA: 60S ribosomal protein L27-A

AB: 60S ribosomal protein L28

AC: 60S ribosomal protein L29

AD: 60S ribosomal protein L30

AE: 60S ribosomal protein L31-A

AF: 60S ribosomal protein L32

AG: 60S ribosomal protein L33-A

AH: 60S ribosomal protein L34-A

AI: 60S ribosomal protein L35-A

AJ: 60S ribosomal protein L36-A

AK: 60S ribosomal protein L37-A

AL: 60S ribosomal protein L38

AM: 60S ribosomal protein L39

AN: Ubiquitin-60S ribosomal protein L40

AO: 60S ribosomal protein L41-B

AP: 60S ribosomal protein L42-A

AQ: 60S ribosomal protein L43-A

i: Suppressor protein STM1

A: 18S ribosomal RNA

B: 40S ribosomal protein S0-A

C: 40S ribosomal protein S1-A

D: 40S ribosomal protein S2

E: 40S ribosomal protein S3

F: 40S ribosomal protein S4-A

G: 40S ribosomal protein S5

H: 40S ribosomal protein S6-A

I: 40S ribosomal protein S7-A

J: 40S ribosomal protein S8-A

K: 40S ribosomal protein S9-A

L: 40S ribosomal protein S10-A

M: 40S ribosomal protein S11-A

N: 40S ribosomal protein S12

O: 40S ribosomal protein S13

P: 40S ribosomal protein S14-B

Q: 40S ribosomal protein S15

R: 40S ribosomal protein S16-A

S: 40S ribosomal protein S17-B

T: 40S ribosomal protein S18-A

U: 40S ribosomal protein S19-A

V: 40S ribosomal protein S20

W: 40S ribosomal protein S21-A

X: 40S ribosomal protein S22-A

Y: 40S ribosomal protein S23-A

Z: 40S ribosomal protein S24-A

a: 40S ribosomal protein S25-A

b: 40S ribosomal protein S26-B

c: 40S ribosomal protein S27-A

d: 40S ribosomal protein S28-A

e: 40S ribosomal protein S29-A

f: 40S ribosomal protein S30-A

g: Ubiquitin-40S ribosomal protein S31

h: Guanine nucleotide-binding protein subunit beta-like protein

hetero molecules

Summary:

• defined by author
• 3.21 MDa, 79 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	3,212,494	1278
Polymers	3,047,221	79
Non-polymers	165,272	1199
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

2

6: 18S ribosomal RNA

AR: 25S ribosomal RNA

AS: 5S ribosomal RNA

AT: 5.8S ribosomal RNA

CD: 60S ribosomal protein L2-A

CE: 60S ribosomal protein L3

CF: 60S ribosomal protein L4-A

CG: 60S ribosomal protein L5

CH: 60S ribosomal protein L6-A

CI: 60S ribosomal protein L7-A

CJ: 60S ribosomal protein L8-A

CK: 60S ribosomal protein L9-A

CL: 60S ribosomal protein L10

CM: 60S ribosomal protein L11-B

CN: 60S ribosomal protein L13-A

CO: 60S ribosomal protein L14-A

CP: 60S ribosomal protein L15-A

CQ: 60S ribosomal protein L16-A

CR: 60S ribosomal protein L17-A

CS: 60S ribosomal protein L18-A

CT: 60S ribosomal protein L19-A

CU: 60S ribosomal protein L20-A

CV: 60S ribosomal protein L21-A

CW: 60S ribosomal protein L22-A

CX: 60S ribosomal protein L23-A

CY: 60S ribosomal protein L24-A

CZ: 60S ribosomal protein L25

DA: 60S ribosomal protein L26-A

DB: 60S ribosomal protein L27-A

DC: 60S ribosomal protein L28

DD: 60S ribosomal protein L29

DE: 60S ribosomal protein L30

DF: 60S ribosomal protein L31-A

DG: 60S ribosomal protein L32

DH: 60S ribosomal protein L33-A

DI: 60S ribosomal protein L34-A

DJ: 60S ribosomal protein L35-A

DK: 60S ribosomal protein L36-A

DL: 60S ribosomal protein L37-A

DM: 60S ribosomal protein L38

DN: 60S ribosomal protein L39

DO: Ubiquitin-60S ribosomal protein L40

DP: 60S ribosomal protein L41-B

DQ: 60S ribosomal protein L42-A

DR: 60S ribosomal protein L43-A

p0: 60S acidic ribosomal protein P0

sM: Suppressor protein STM1,Suppressor protein STM1,Suppressor protein Stm1 - Mol B

s0: 40S ribosomal protein S0-A

s1: 40S ribosomal protein S1-A

s2: 40S ribosomal protein S2

s3: 40S ribosomal protein S3

s4: 40S ribosomal protein S4-A

s5: 40S ribosomal protein S5

s6: 40S ribosomal protein S6-A

s7: 40S ribosomal protein S7-A

s8: 40S ribosomal protein S8-A

s9: 40S ribosomal protein S9-A

c0: 40S ribosomal protein S10-A

c1: 40S ribosomal protein S11-A

c2: 40S ribosomal protein S12

c3: 40S ribosomal protein S13

c4: 40S ribosomal protein S14-B

c5: 40S ribosomal protein S15

c6: 40S ribosomal protein S16-A

c7: 40S ribosomal protein S17-A

c8: 40S ribosomal protein S18-A

c9: 40S ribosomal protein S19-A

d0: 40S ribosomal protein S20

d1: 40S ribosomal protein S21-A

d2: 40S ribosomal protein S22-A

d3: 40S ribosomal protein S23-A

d4: 40S ribosomal protein S24-A

d5: 40S ribosomal protein S25-A

d6: 40S ribosomal protein S26-B

d7: 40S ribosomal protein S27-A

d8: 40S ribosomal protein S28-A

d9: 40S ribosomal protein S29-A

e0: 40S ribosomal protein S30-A

e1: Ubiquitin-40S ribosomal protein S31

sR: Guanine nucleotide-binding protein subunit beta-like protein

hetero molecules

Summary:

• defined by author
• 3.24 MDa, 80 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	3,237,666	1375
Polymers	3,060,627	80
Non-polymers	177,040	1295
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Unit cell

Length a, b, c (Å)	304.080, 286.550, 436.550
Angle α, β, γ (deg.)	90.000, 99.050, 90.000
Int Tables number	4
Space group name H-M	P1211

Components

RNA chain , 4 types, 8 molecules 1 AR 3 AS 4 AT 6 A

#1: RNA chain

1 AR 25S ribosomal RNA

Details:

Mass: 1097493.875 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Production host: Saccharomyces cerevisiae (brewer's yeast) / References: REF: 831416132

#2: RNA chain

3 AS 5S ribosomal RNA

Details:

Mass: 38951.105 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Production host: Saccharomyces cerevisiae (brewer's yeast) / References: GenBank: 1039024062

#3: RNA chain

4 AT 5.8S ribosomal RNA

Details:

Mass: 50682.922 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Production host: Saccharomyces cerevisiae (brewer's yeast) / References: GenBank: 1102645687

#25: RNA chain

6 A 18S ribosomal RNA

Details:

Mass: 579761.938 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Production host: Saccharomyces cerevisiae (brewer's yeast) / References: GenBank: 874346701

60S ribosomal protein ... , 40 types, 80 molecules j CD k CE l CF m CG n CH o CI p CJ q CK r CL s CM t CN u CO v CP w CQ x CR ...

#4: Protein

j CD 60S ribosomal protein L2-A / L5 / RP8 / YL6

Details:

Mass: 27217.289 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL2A, RPL5B, YFR031C-A, YFR031BC / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX45

#5: Protein

k CE 60S ribosomal protein L3 / Maintenance of killer protein 8 / RP1 / Trichodermin resistance protein / YL1

Details:

Mass: 43719.602 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL3, MAK8, TCM1, YOR063W, YOR29-14 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P14126

#6: Protein

l CF 60S ribosomal protein L4-A / L2 / RP2 / YL2

Details:

Mass: 39027.926 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL4A, RPL2, RPL2A, YBR031W, YBR0315 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P10664

#7: Protein

m CG 60S ribosomal protein L5 / L1 / L1a / Ribosomal 5S RNA-binding protein / YL3

Details:

Mass: 33633.633 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL5, RPL1, RPL1A, YPL131W, LPI14W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P26321

#8: Protein

n CH 60S ribosomal protein L6-A / L17 / RP18 / YL16

Details:

Mass: 19869.369 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL6A, RPL17A, YL16A, YML073C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q02326

#9: Protein

o CI 60S ribosomal protein L7-A / L6 / RP11 / YL8

Details:

Mass: 25240.377 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL7A, RPL6A, RPL8A, YL8A, YGL076C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05737

#10: Protein

p CJ 60S ribosomal protein L8-A / L4 / L4-2 / L7a-1 / Maintenance of killer protein 7 / RP6 / YL5

Details:

Mass: 25814.998 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL8A, MAK7, RPL4A, YHL033C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P17076

#11: Protein

q CK 60S ribosomal protein L9-A / L8 / RP24 / YL11

Details:

Mass: 21605.061 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL9A, RPL8A, RPL9, YGL147C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05738

#12: Protein

r CL 60S ribosomal protein L10 / L9 / Ubiquinol-cytochrome C reductase complex subunit VI-requiring protein

Details:

Mass: 25279.271 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL10, GRC5, QSR1, YLR075W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P41805

#13: Protein

s CM 60S ribosomal protein L11-B / L16 / RP39 / YL22

Details:

Mass: 19266.082 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL11B, RP39B, RPL16B, YGR085C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q3E757

#14: Protein

t CN 60S ribosomal protein L13-A

Details:

Mass: 21885.234 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL13A, YDL082W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q12690

#15: Protein

u CO 60S ribosomal protein L14-A

Details:

Mass: 14976.794 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL14A, YKL006W, YKL153 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P36105

#16: Protein

v CP 60S ribosomal protein L15-A / L13 / RP15R / YL10 / YP18

Details:

Mass: 24351.162 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL15A, RPL10A, RPL13A, YL10A, YLR029C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05748

#17: Protein

w CQ 60S ribosomal protein L16-A / L13a / L21 / RP22 / YL15

Details:

Mass: 22028.951 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL16A, RPL13, RPL21A, YIL133C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P26784

#18: Protein

x CR 60S ribosomal protein L17-A / L20A / YL17

Details:

Mass: 20458.322 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL17A, RPL17, RPL20A, YKL180W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05740

#19: Protein

y CS 60S ribosomal protein L18-A / RP28

Details:

Mass: 20478.059 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL18A, RP28A, YOL120C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX49

#20: Protein

z CT 60S ribosomal protein L19-A / L23 / RP15L / RP33 / YL14

Details:

Mass: 21631.121 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL19A, RPL23A, YL14A, YBR084C-A, YBR084BC / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX82

#21: Protein

0 CU 60S ribosomal protein L20-A / L18a

Details:

Mass: 20478.852 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL20A, RPL18A, RPL18A2, YMR242C, YM9408.04C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX23

#22: Protein

2 CV 60S ribosomal protein L21-A

Details:

Mass: 18148.066 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL21A, URP1, YBR191W, YBR1401 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q02753

#23: Protein

5 CW 60S ribosomal protein L22-A / L1c / RP4 / YL31

Details:

Mass: 11263.863 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL22A, YLR061W, L2168 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05749

#24: Protein

l2 CX 60S ribosomal protein L23-A / L17a / YL32

Details:

Mass: 14362.755 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL23A, RPL17A, RPL17AA, YBL087C, YBL0713 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX41

#26: Protein

7 CY 60S ribosomal protein L24-A / L30 / RP29 / YL21

Details:

Mass: 11369.424 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL24A, RP29, RPL30A, YGL031C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P04449

#27: Protein

8 CZ 60S ribosomal protein L25 / RP16L / YL25 / YP42'

Details:

Mass: 13771.176 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL25, YOL127W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P04456

#28: Protein

9 DA 60S ribosomal protein L26-A / L33 / YL33

Details:

Mass: 14134.588 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL26A, RPL26, RPL33A, YLR344W, L8300.4 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05743

#29: Protein

AA DB 60S ribosomal protein L27-A

Details:

Mass: 15437.166 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL27A, RPL27, YHR010W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0C2H6

#30: Protein

AB DC 60S ribosomal protein L28 / L27a / L29 / RP44 / RP62 / YL24

Details:

Mass: 16630.471 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL28, CYH2, YGL103W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P02406

#31: Protein

AC DD 60S ribosomal protein L29 / YL43

Details:

Mass: 6560.688 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL29, YL43, YFR032C-A, YFR032BW / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05747

#32: Protein

AD DE 60S ribosomal protein L30 / L32 / RP73 / YL38

Details:

Mass: 10558.344 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL30, RPL32, YGL030W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P14120

#33: Protein

AE DF 60S ribosomal protein L31-A / L34 / YL28

Details:

Mass: 12649.754 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL31A, RPL34, RPL34A, YDL075W, D2478 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0C2H8

#34: Protein

AF DG 60S ribosomal protein L32

Details:

Mass: 14478.054 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL32, YBL092W, YBL0838 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P38061

#35: Protein

AG DH 60S ribosomal protein L33-A / L37 / RP47 / YL37

Details:

Mass: 12045.935 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL33A, RPL37A, YPL143W, LPI4W, P2625 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05744

#36: Protein

AH DI 60S ribosomal protein L34-A

Details:

Mass: 12608.831 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL34A, YER056C-A, YER056BC / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P87262

#37: Protein

AI DJ 60S ribosomal protein L35-A

Details:

Mass: 13811.444 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL35A, SOS1, YDL191W, D1249 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX84

#38: Protein

AJ DK 60S ribosomal protein L36-A / L39 / YL39

Details:

Mass: 11020.063 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL36A, RPL39A, YMR194W, YM9646.06 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05745

#39: Protein

AK DL 60S ribosomal protein L37-A / L43 / YL35 / YP55

Details:

Mass: 9746.199 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL37A, RPL35A, YLR185W, L9470.6 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P49166

#40: Protein

AL DM 60S ribosomal protein L38

Details:

Mass: 8714.363 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL38, YLR325C, L8543.2 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P49167

#41: Protein/peptide

AM DN 60S ribosomal protein L39 / L46 / YL40

Details:

Mass: 6227.444 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL39, RPL46, SPB2, YJL189W, J0360 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P04650

#43: Protein/peptide

AO DP 60S ribosomal protein L41-B / L47 / YL41

Details:

Mass: 3354.243 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL41B, RPL47B, YL41B, YDL133C-A, YDL133BC / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX87, UniProt: P0CX86*PLUS

#44: Protein

AP DQ 60S ribosomal protein L42-A / L41 / YL27 / YP44

Details:

Mass: 12115.462 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL42A, RPL41A, SCL41A, YNL162W, N1722 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX27

#45: Protein

AQ DR 60S ribosomal protein L43-A / L37a / YL35

Details:

Mass: 9981.756 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL43A, YPR043W, YP9499.02 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX25

Protein , 3 types, 5 molecules AN DO p0 h sR

#42: Protein

AN DO Ubiquitin-60S ribosomal protein L40

Details:

Mass: 6032.321 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPL40A, UBI1, YIL148W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CH08

#47: Protein

p0 60S acidic ribosomal protein P0 / A0 / L10E

Details:

Mass: 24101.676 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPP0, L10E, RPA0, RPL10E, RPLA0, YLR340W, L8300.8 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05317

#81: Protein

h sR Guanine nucleotide-binding protein subunit beta-like protein / Receptor for activated C kinase / Receptor of activated protein kinase C 1 / RACK1

Details:

Mass: 34710.023 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: ASC1, CPC2, YMR116C, YM9718.15C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P38011

Suppressor protein ... , 2 types, 2 molecules i sM

#46: Protein

i Suppressor protein STM1 / 3BP1 / GU4 nucleic-binding protein 2 / G4p2 protein / POP2 multicopy suppressor protein 4 / Ribosomal subunits association factor / AF / TOM1 suppressor protein 1 / Triplex-binding protein 1

Details:

Mass: 18224.963 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: STM1, MPT4, STO1, YLR150W, L9634.1 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P39015

#48: Protein

sM Suppressor protein STM1,Suppressor protein STM1,Suppressor protein Stm1 - Mol B / 3BP1 / GU4 nucleic-binding protein 2 / G4p2 protein / POP2 multicopy suppressor protein 4 / Ribosomal subunits association factor / AF / TOM1 suppressor protein 1 / Triplex-binding protein 1

Details:

Mass: 10296.888 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: STM1, MPT4, STO1, YLR150W, L9634.1 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P39015

40S ribosomal protein ... , 32 types, 62 molecules B s0 C s1 D s2 E s3 F s4 G s5 H s6 I s7 J s8 K s9 L c0 M c1 N c2 O c3 P c4 ...

#49: Protein

B s0 40S ribosomal protein S0-A / Nucleic acid-binding protein NAB1A

Details:

Mass: 22811.074 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS0A, NAB1, NAB1A, YST1, YGR214W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P32905

#50: Protein

C s1 40S ribosomal protein S1-A / RP10A

Details:

Mass: 24498.365 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS1A, PLC1, RPS10A, YLR441C, L9753.9 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P33442

#51: Protein

D s2 40S ribosomal protein S2 / Omnipotent suppressor protein SUP44 / RP12 / S4 / YS5

Details:

Mass: 23212.979 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS2, RPS4, SUP38, SUP44, YGL123W, G2893 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P25443

#52: Protein

E s3 40S ribosomal protein S3 / RP13 / YS3

Details:

Mass: 24702.791 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS3, SUF14, YNL178W, N1653 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05750

#53: Protein

F s4 40S ribosomal protein S4-A / RP5 / S7 / YS6

Details:

Mass: 29338.133 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS4A, RPS7B, YJR145C, J2186 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX35

#54: Protein

G s5 40S ribosomal protein S5 / RP14 / S2 / YS8

Details:

Mass: 22908.338 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS5, RPS2, YJR123W, J2045 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P26783

#55: Protein

H s6 40S ribosomal protein S6-A / RP9 / S10 / YS4

Details:

Mass: 25895.072 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS6A, RPS101, RPS10B, YPL090C, LPG18C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX37

#56: Protein

I s7 40S ribosomal protein S7-A / RP30 / RP40

Details:

Mass: 21158.648 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS7A, RPS30, YOR096W, YOR3177W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P26786

#57: Protein

J s8 40S ribosomal protein S8-A / RP19 / S14 / YS9

Details:

Mass: 22406.609 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS8A, RPS14A, YBL072C, YBL06.05, YBL0613 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX39

#58: Protein

K s9 40S ribosomal protein S9-A / RP21 / S13 / YP28 / YS11

Details:

Mass: 21210.662 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS9A, RPS13A, YS11A, YPL081W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: O13516

#59: Protein

L c0 40S ribosomal protein S10-A

Details:

Mass: 12757.445 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS10A, YOR293W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q08745

#60: Protein

M c1 40S ribosomal protein S11-A / RP41 / S18 / YS12

Details:

Mass: 17654.738 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS11A, RPS18A, YDR025W, YD9813.03 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX47

#61: Protein

N c2 40S ribosomal protein S12

Details:

Mass: 13327.331 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS12, YOR369C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P48589

#62: Protein

O c3 40S ribosomal protein S13 / S27a / YS15

Details:

Mass: 16928.748 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS13, RPS13C, YDR064W, D4252, YD9609.18 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05756

#63: Protein

P c4 40S ribosomal protein S14-B / RP59B

Details:

Mass: 13560.528 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS14B, CRY2, YJL191W, J0354 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P39516

#64: Protein

Q c5 40S ribosomal protein S15 / RIG protein / RP52 / S21 / YS21

Details:

Mass: 15900.712 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS15, RPS21, YOL040C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q01855

#65: Protein

R c6 40S ribosomal protein S16-A / RP61R

Details:

Mass: 15746.292 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS16A, RP61R, YMR143W, YM9375.12 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX51

#66: Protein

S 40S ribosomal protein S17-B / RP51B

Details:

Mass: 14268.545 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS17B, RP51B, YDR447C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P14127

#67: Protein

T c8 40S ribosomal protein S18-A

Details:

Mass: 16940.443 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS18A, YDR450W, D9461.35 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX55

#68: Protein

U c9 40S ribosomal protein S19-A / RP55A / S16a / YP45 / YS16A

Details:

Mass: 15810.930 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS19A, RP55A, RPS16AA, YOL121C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P07280

#69: Protein

V d0 40S ribosomal protein S20

Details:

Mass: 12575.547 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS20, URP2, YHL015W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P38701

#70: Protein

W d1 40S ribosomal protein S21-A / S26 / YS25

Details:

Mass: 9758.829 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS21A, RPS25, RPS25A, RPS26A, YKR057W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0C0V8

#71: Protein

X d2 40S ribosomal protein S22-A / RP50 / S24 / YP58 / YS22

Details:

Mass: 14518.867 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS22A, RPS24, RPS24A, YJL190C, J0355 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0C0W1

#72: Protein

Y d3 40S ribosomal protein S23-A / RP37 / S28 / YS14

Details:

Mass: 15942.699 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS23A, RPS28A, YGR118W, G6178 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX29

#73: Protein

Z d4 40S ribosomal protein S24-A / RP50

Details:

Mass: 15231.650 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS24A, RPS24EA, YER074W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX31

#74: Protein

a d5 40S ribosomal protein S25-A / RP45 / S31 / YS23

Details:

Mass: 8001.377 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS25A, RPS31A, YGR027C / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q3E792

#75: Protein

b d6 40S ribosomal protein S26-B

Details:

Mass: 11022.989 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS26B, YER131W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P39939

#76: Protein

c d7 40S ribosomal protein S27-A / RP61 / YS20

Details:

Mass: 8762.195 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS27A, YKL156W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P35997

#77: Protein

d d8 40S ribosomal protein S28-A / S33 / YS27

Details:

Mass: 7116.281 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS28A, RPS33, RPS33A, YOR167C, O3600 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q3E7X9

#78: Protein

e d9 40S ribosomal protein S29-A / S36 / YS29

Details:

Mass: 6335.303 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS29A, RPS36A, YS29A, YLR388W, L8084.11 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P41057

#79: Protein

f e0 40S ribosomal protein S30-A

Details:

Mass: 7006.346 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS30A, YLR287C-A, L8003.23, YLR287BC / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX33

#82: Protein

c7 40S ribosomal protein S17-A / RP51A

Details:

Mass: 13897.153 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS17A, RP51A, YML024W / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P02407

Ubiquitin-40S ribosomal protein ... , 2 types, 2 molecules g e1

#80: Protein

g Ubiquitin-40S ribosomal protein S31

Details:

Mass: 8101.675 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS31, RPS37, UBI3, YLR167W, L9470.14 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05759

#83: Protein

e1 Ubiquitin-40S ribosomal protein S31

Details:

Mass: 5704.610 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Gene: RPS31, RPS37, UBI3, YLR167W, L9470.14 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05759

Non-polymers , 4 types, 2494 molecules

#84: Chemical

1-3401 1-3402 1-3403 1-3404 1-3405 1-3406 1-3407 1-3408 1-3409 1-3410 1-3411 1-3412 1-3413 1-3414 1-3415 1-3416 1-3417 1-3418 1-3419 1-3420 ...
ChemComp-OHX / osmium (III) hexammine / osmium(6+) hexaazanide

Details:

Mass: 286.365 Da / Num. of mol.: 1070 / Source method: obtained synthetically / Formula: H₁₂N₆Os

#85: Chemical

1-3732 1-3733 1-3734 1-3735 1-3736 1-3737 1-3738 1-3739 1-3740 1-3741 1-3742 1-3743 1-3744 1-3745 1-3746 1-3747 1-3748 1-3749 1-3750 1-3751 ...
ChemComp-MG / MAGNESIUM ION

Details:

Mass: 24.305 Da / Num. of mol.: 1406 / Source method: obtained synthetically / Formula: Mg

#86: Chemical

1-4216 AR-4239 ChemComp-7MB / Agelastatin A

Details:

Mass: 341.161 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C₁₂H₁₃BrN₄O₃

#87: Chemical

AK-101 AN-500 AP-501 AQ-501 DL-103 DO-201 DQ-501 DR-501 b-102 c-101 e-102 g-501 d6-102 d7-101 d9-103 e1-501
ChemComp-ZN / ZINC ION

Details:

Mass: 65.409 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: Zn

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 3.18 ų/Da / Density % sol: 61.3 %
• Crystal grow: Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7 ; Details: PEG 20K, KSCN, Mg Acetate, Tris-Acetate, Glycerol, Spermidine

Data collection

Diffraction

ID	Mean temperature (K)	Crystal-ID
1	100	1
2		1

Diffraction source

Source	Site	Beamline	ID	Wavelength (Å)
SYNCHROTRON	SOLEIL	PROXIMA 1	1	1.148
SYNCHROTRON	SLS	X06SA	2	1

Detector

Type	ID	Detector	Date
DECTRIS PILATUS 6M	1	PIXEL	Mar 12, 2016
DECTRIS EIGER X 16M	2	PIXEL	Jun 12, 2016

Radiation

ID	Protocol	Monochromatic (M) / Laue (L)	Scattering type	Wavelength-ID
1	SINGLE WAVELENGTH	M	x-ray	1
2	SINGLE WAVELENGTH	M	x-ray	2

Radiation wavelength

ID	Wavelength (Å)	Relative weight
1	1.148	1
2	1	1

• Reflection: Resolution: 3.5→98.383 Å / Num. obs: 925728 / % possible obs: 100 % / Observed criterion σ(I): -3 / Redundancy: 10.553 % / B_iso Wilson estimate: 71.28 Ų / CC_1/2: 0.985 / R_merge(I) obs: 0.567 / R_rim(I) all: 0.597 / Χ²: 0.898 / Net I/σ(I): 5.56 / Num. measured all: 9768849 / Scaling rejects: 1016

Reflection shell

Diffraction-ID: 1

Resolution (Å)	Redundancy (%)	Rmerge(I) obs	Mean I/σ(I) obs	Num. measured obs	Num. possible	Num. unique obs	CC1/2	Rrim(I) all	% possible all
3.5-3.6	7.424	2.259	1	555104	74868	74772	0.322	2.425	99.9
3.6-3.7	7.416	1.821	1.25	496145	66935	66902	0.419	1.956	100
3.7-3.8	7.561	1.451	1.58	455091	60205	60189	0.53	1.557	100
3.8-3.9	9.491	1.494	1.93	513106	54065	54063	0.617	1.579	100
3.9-4	9.54	1.288	2.24	465356	48796	48779	0.687	1.361	100
4-4.5	9.414	0.892	3.23	1734865	184329	184295	0.812	0.944	100
4.5-5	9.454	0.568	4.96	1115179	117969	117958	0.913	0.601	100
5-7.5	12.624	0.527	7.81	2824395	223753	223727	0.963	0.548	100
7.5-10	17.331	0.327	14.55	948483	54735	54727	0.991	0.337	100
10-15	16.523	0.17	20.87	467079	28273	28269	0.996	0.175	100
15-20	16.37	0.123	24.96	113279	6922	6920	0.997	0.127	100
20-25	16.432	0.086	32.52	41145	2505	2504	0.999	0.089	100
25-30	16.268	0.067	39.89	18057	1110	1110	0.999	0.07	100
30-40	16.363	0.063	45.24	14547	890	889	0.999	0.065	99.9
40-50	15.121	0.063	48.32	4869	322	322	0.999	0.065	100
50-75	7.309	0.031	42.26	1798	246	246	0.999	0.034	100
75-100	6.268	0.033	44.14	351	61	56	1	0.037	91.8
98.383-100					49

Processing

Software

Name	Version	Classification
PHENIX		refinement
XSCALE		data scaling
PDB_EXTRACT	3.2	data extraction
PHASER		phasing
XDS		data reduction

Refinement

Method to determine structure: MOLECULAR REPLACEMENT
Starting model: 4V88

4v88

Resolution: 3.5→98.383 Å / SU ML: 0.43 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 22.29 / Stereochemistry target values: ML

	Rfactor	Num. reflection	% reflection
Rfree	0.239	18297	1.98 %
Rwork	0.1953	907348	-
obs	0.1962	925645	99.97 %

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
• Displacement parameters: B_iso max: 320.72 Ų / B_iso mean: 79.7288 Ų / B_iso min: 0 Ų

Refinement step

Cycle: final / Resolution: 3.5→98.383 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	178134	222512	8952	0	409598
Biso mean	-	-	149.26	-	-
Num. residues	-	-	-	-	33045

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.007	436643
X-RAY DIFFRACTION	f_angle_d	1.13	647761
X-RAY DIFFRACTION	f_chiral_restr	0.046	79231
X-RAY DIFFRACTION	f_plane_restr	0.006	41536
X-RAY DIFFRACTION	f_dihedral_angle_d	16.915	194591

LS refinement shell

Refine-ID: X-RAY DIFFRACTION / R_factor R_free error: 0 / Total num. of bins used: 30 / % reflection obs: 100 %

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	Num. reflection all
3.5-3.5398	0.3352	602	0.3088	30219	30821
3.5398-3.5814	0.3349	642	0.2986	30135	30777
3.5814-3.6251	0.3296	615	0.2915	30119	30734
3.6251-3.671	0.2958	638	0.2722	30096	30734
3.671-3.7193	0.296	571	0.2603	30182	30753
3.7193-3.7702	0.2724	599	0.2444	30209	30808
3.7702-3.8241	0.2871	617	0.2412	30076	30693
3.8241-3.8812	0.2674	644	0.2308	30161	30805
3.8812-3.9418	0.2625	573	0.2196	30240	30813
3.9418-4.0065	0.2771	579	0.2229	30223	30802
4.0065-4.0756	0.2617	626	0.2046	30111	30737
4.0756-4.1497	0.2291	587	0.1974	30207	30794
4.1497-4.2295	0.2358	598	0.1928	30263	30861
4.2295-4.3158	0.2292	613	0.1883	30193	30806
4.3158-4.4097	0.2297	569	0.1817	30269	30838
4.4097-4.5122	0.2405	648	0.18	30074	30722
4.5122-4.6251	0.2187	606	0.1723	30275	30881
4.6251-4.7501	0.2144	628	0.1657	30220	30848
4.7501-4.8899	0.2076	574	0.1617	30165	30739
4.8899-5.0477	0.2196	627	0.1622	30296	30923
5.0477-5.2281	0.1988	638	0.154	30246	30884
5.2281-5.4374	0.2073	586	0.1526	30239	30825
5.4374-5.6849	0.2081	668	0.1561	30186	30854
5.6849-5.9846	0.2096	612	0.1544	30346	30958
5.9846-6.3594	0.2033	620	0.1551	30256	30876
6.3594-6.8504	0.2248	614	0.1644	30357	30971
6.8504-7.5395	0.2179	576	0.1649	30351	30927
7.5395-8.6299	0.2142	591	0.1723	30403	30994
8.6299-10.8706	0.2201	603	0.1838	30478	31081
10.8706-98.4216	0.2358	633	0.218	30753	31386

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.1166	0.0164	-0.0565	0.1299	-0.031	0.1813	-0.0124	0.0009	-0.0319	0.0192	-0.0574	-0.0559	0.0149	0.0477	-0.7524	0.2259	0.0057	0.0036	0.2827	0.0769	0.2221	68.2847	-1.3386	47.9395
2	0.0762	0.0237	0.187	0.2322	0.0715	0.1909	-0.1053	0.0469	0.156	0.0003	-0.0318	0.3532	-0.324	-0.3344	-0.6822	0.6329	0.3403	-0.1761	0.463	0.163	0.4629	12.8052	67.246	26.2437
3	0.2021	0.0767	0.1097	0.0528	0.0187	0.2071	0.0065	-0.2207	-0.0278	0.1297	-0.0847	-0.2029	-0.0206	0.2874	-0.2856	0.2724	0.012	-0.1165	0.7178	0.2792	0.5193	114.9819	-7.1213	82.3612
4	0.172	0.0271	0.0149	0.13	0.1027	0.0594	0.0339	0.1417	0.0791	-0.0493	-0.1136	-0.0826	-0.0987	0.1144	-0.1455	0.2766	0.071	0.1429	0.554	0.1877	0.4676	123.1849	4.3134	16.5455
5	0.1446	-0.0777	0.0875	0.0496	-0.0242	0.0592	-0.0848	0.0197	-0.1664	-0.064	0.0273	-0.0541	0.1931	-0.058	-0.0002	0.5432	-0.1017	0.1554	0.316	-0.1015	0.4233	33.1776	-60.5034	37.8208
6	0.0903	0.0923	-0.0565	0.2916	-0.0221	0.1845	0.0759	-0.0836	-0.0205	0.1949	-0.1349	-0.1251	-0.1005	0.0459	-0.1121	0.4724	-0.0756	0.0021	0.4204	-0.0206	0.1923	58.1012	25.1599	102.8307
7	0.0107	0.0232	0.0439	0.1076	0.0461	0.0211	-0.126	0.1693	0.3688	-0.0553	-0.0705	0.1541	-0.2177	-0.0053	-0.2548	0.8299	0.1759	-0.2141	0.3334	0.2438	0.7126	26.0469	93.4571	28.8163
8	0.0445	-0.0125	-0.0024	0.0683	-0.0176	0.0004	0.0996	0.0936	-0.0618	0.2453	-0.0412	0.0615	0.0121	-0.1416	0	0.4591	-0.0445	0.1624	0.5144	0.009	0.3556	-4.1387	-11.5391	106.534
9	0.0444	0.0123	-0.0282	0.0189	-0.0369	0.0548	0.0034	0.0794	0.1435	0.0477	0.0755	0.0336	0.1421	-0.0886	0.0048	0.3347	0.1058	0.0631	0.3955	0.013	0.3525	4.9021	31.7891	77.4502
10	0.0718	-0.1223	-0.0214	0.1432	0.0091	-0.0026	0.0579	0.018	-0.0211	-0.0952	-0.0645	-0.1618	-0.1004	0.2178	-0.0105	0.4487	-0.2717	-0.045	0.9405	0.2315	0.8301	154.6376	40.1132	53.3069
11	0.0104	-0.0397	0.0208	0.1754	-0.0641	0.0329	-0.0768	0.0905	-0.0226	-0.0926	0.1208	0.1919	0.2203	-0.1502	0.0199	0.4933	-0.2259	-0.0576	0.8207	-0.1257	0.3926	-11.9683	-32.4917	18.9196
12	0.0512	0.001	-0.0632	0.0455	-0.025	0.0797	0.014	0.0782	0.0135	-0.1657	-0.0089	0.1174	-0.0175	-0.1083	-0.0029	0.4567	-0.0288	-0.1279	0.6533	0.0751	0.2736	10.4738	27.505	13.2374
13	0.0285	-0.0035	0.0208	0.0492	0.0219	0.0175	-0.1467	0.2352	0.0902	-0.2442	0.0488	-0.0223	-0.215	0.0086	0.0003	1.0097	0.0396	-0.1242	0.738	0.2827	0.6226	31.2848	79.0923	-7.6746
14	0.0295	0.0097	-0.0103	-0.0015	-0.0229	0.004	-0.0052	-0.1053	0.1906	0.0765	-0.0129	-0.1125	-0.0174	0.094	0.0319	0.5234	-0.2467	-0.1993	0.5136	0.0271	0.4548	99.1042	55.34	87.7051
15	0.0183	0.0212	-0.0111	0.0217	-0.0058	0.0454	-0.0197	0.057	-0.0474	0.0438	-0.0608	0.1465	0.2076	-0.1277	0	0.3914	-0.0449	0.073	0.7584	0.0671	0.4953	-26.2181	-13.6453	65.2468
16	0.0804	-0.0058	0.0043	0.0181	0.0548	0.0598	-0.0173	-0.146	0.1369	0.0781	-0.0818	-0.151	-0.0883	0.1214	-0.1058	0.246	-0.1606	-0.1122	0.565	0.2486	0.4299	119.4055	32.4752	61.6115
17	0.0289	0.0344	-0.045	0.0685	-0.0492	0.0451	-0.0526	0.0459	-0.1094	0.0541	0.0118	-0.055	0.1092	-0.1701	-0.0008	0.3493	-0.1212	0.039	0.3932	-0.0793	0.232	3.3739	-29.4594	57.235
18	0.0117	-0.0414	0.023	0.1251	-0.0581	0.0573	-0.0236	0.0043	-0.0023	0.0458	-0.0532	0.1341	0.0827	0.0221	-0.001	0.4766	0.0137	0.0285	0.3155	0.1231	0.4505	55.4773	-39.841	82.8594
19	0.0508	-0.0465	-0.0209	0.0546	-0.0489	0.0812	0.1067	-0.0808	0.1812	0.0881	-0.1657	0.0105	-0.3179	0.0652	-0.0261	0.6206	-0.1335	0.041	0.3414	-0.0315	0.2264	52.1676	50.2101	90.0078
20	0.0403	-0.0414	-0.0352	-0.0499	0.0056	0.0114	0.0152	0.0604	-0.0588	0.0254	-0.0131	-0.1793	0.1691	0.011	-0.0194	0.5846	0.2457	0.2003	0.6779	0.0606	0.7874	121.5537	-57.3581	10.7912
21	0.0586	-0.004	-0.0878	0.0584	-0.0333	0.1078	-0.0505	0.1258	0.1514	-0.227	0.0244	-0.0118	0.0426	-0.2295	0.0004	0.268	0.0293	-0.0222	0.6912	-0.0146	0.4519	-12.344	14.7127	47.4763
22	0.0096	0.0441	-0.0181	0.0894	-0.0215	0.0198	0.0438	-0.0321	0.0216	0.0739	-0.0911	0.1243	0.0597	-0.0521	-0.0064	0.506	0.2559	-0.0593	0.4304	0.0835	0.337	21.9283	55.299	50.1593
23	0.0034	-0.0008	0.0055	0.0074	-0.0015	0.0056	0.0894	-0.0086	-0.1311	0.0533	-0.0353	0.0282	0.0035	-0.003	0	1.118	0.3304	0.0264	0.7872	0.1522	1.2219	113.3981	-89.0905	42.3105
24	0.025	-0.034	0.0149	0.0374	-0.0085	0.0079	-0.1238	0.0183	0.0083	-0.1283	-0.1041	0.11	0.0711	-0.0706	0.0003	0.6555	0.0463	0.0815	0.4539	-0.0872	0.4246	48.8592	-44.8268	-0.1064
25	0.1387	-0.0051	0.014	0.3126	0.1163	0.1335	0.1009	-0.0775	-0.0258	0.1977	-0.0638	-0.0381	0.0764	-0.0325	-0.001	0.6171	-0.134	0.0519	0.3789	0.0596	0.3939	201.5692	-25.1337	274.7438
26	0.003	-0.0011	0.0161	0.0291	-0.0372	0.0381	0.0265	-0.0108	-0.1449	-0.0466	0.0528	0.0247	0.1013	-0.1265	0	0.968	0.009	0.1602	0.5763	-0.0861	0.6478	57.0866	-80.5972	3.8691
27	0.0321	-0.0361	0.0071	0.0365	0.0129	0.0729	-0.0472	0.096	0.0167	0.0418	0.057	-0.0975	-0.0237	0.1802	0.0079	0.3245	0.1568	-0.0711	0.879	0.264	0.7512	133.3737	-24.4157	74.0698
28	0.0532	-0.0333	-0.0307	0.0266	0.02	0.0193	-0.0221	-0.053	-0.0768	0.1301	0.0799	0.0385	0.08	0.1192	-0.0157	0.5088	-0.0923	-0.1747	0.8289	0.2724	0.4753	96.9644	-11.1182	117.3636
29	0.031	-0.0088	-0.0056	0.0075	-0.0118	0.019	-0.0445	-0.0412	-0.0055	0.0582	-0.0691	-0.141	-0.0425	0.1447	-0.0005	0.5126	0.2445	0.1712	1.2434	0.1003	1.2075	172.8056	-26.9893	19.4152
30	0.0816	0.0166	-0.0703	0.1314	-0.0865	0.0779	0.0076	-0.0899	0.0728	0.1262	0.0044	0.0425	-0.1903	-0.0284	0.0047	0.5486	-0.1719	-0.047	0.4451	-0.0666	0.2422	72.5243	54.4205	78.71
31	0.0175	-0.0401	0.0366	0.0202	-0.02	0.0369	-0.0066	0.0302	0.121	0.0152	-0.0156	-0.0125	0.041	0.0655	0	0.4499	0.0962	-0.0111	0.4124	0.0422	0.3548	41.8907	56.6207	63.3625
32	0.0062	0.0104	-0.0016	0.0078	-0.0023	0.0045	-0.0448	-0.0286	0.0732	-0.0064	0.0424	-0.0195	0.0154	0.0428	0	0.7846	0.1271	0.2536	1.0898	0.1387	0.9348	155.7822	-23.0008	-8.311
33	0.0157	0.0055	-0.0109	0.0061	-0.0087	0.0095	0.0885	-0.0165	0.0037	0.1111	-0.0117	-0.0558	0.0839	0.0277	-0.0001	0.6413	0.1143	0.1285	0.3855	0.0687	0.6157	73.0743	-71.3924	56.1114
34	0.0788	0.0521	0.0025	0.0325	-0.0053	0.0162	-0.0081	-0.2134	-0.1794	0.1136	-0.0154	-0.043	0.026	-0.0364	0.0007	0.4032	-0.1081	0.0088	0.3659	0.0094	0.2723	43.0761	0.6531	97.5509
35	0.004	0.0174	-0.0032	0.0472	0.0041	0.003	-0.0007	-0.0246	0.0729	0.2338	0.0889	-0.133	0.0203	0.0748	0.0082	0.2997	-0.036	0.0709	0.5004	0.0307	0.2649	9.204	-13.7115	83.9669
36	0.0266	-0.0179	-0.0276	0.0297	0.0275	0.0074	-0.0975	-0.1024	-0.0134	0.0501	0.0159	-0.1633	0.0315	0.0877	0	0.5304	0.2465	0.0488	0.7995	0.1756	0.8364	141.6337	-26.977	28.515
37	0.1431	-0.0161	-0.131	0.0066	0.0188	0.118	-0.1167	-0.0474	-0.0486	0.1002	-0.0244	-0.0327	-0.0253	0.2371	-0.0291	0.3941	-0.03	-0.1573	0.822	0.1352	0.5877	128.5954	1.1393	94.4724
38	0.015	0.0035	-0.0269	0.0415	-0.0207	0.0208	-0.051	0.0737	0.1142	0.0234	-0.064	-0.2	-0.1067	0.1124	0.0001	0.6035	-0.2377	-0.1036	0.6851	0.2711	0.7231	120.1821	63.9593	55.8712
39	0.0338	-0.0173	-0.0091	0.0452	-0.0209	0.0148	-0.0658	0.0031	0.1008	0.0572	-0.0774	-0.1299	0.154	0.0701	-0	0.4227	0.0226	-0.0943	0.4946	0.152	0.4684	108.0474	-2.2514	71.0092
40	0	-0.0008	0.0007	0.0074	-0.0034	0.0043	-0.0538	0.0561	-0.0568	-0.0259	-0.0919	-0.0167	0.0309	-0.0565	0.0002	0.7769	0.3661	-0.0106	0.9676	0.1251	1.0072	148.8457	-61.1694	52.7362
41	0.0128	-0.0147	0.0085	0.0184	-0.0038	0.009	-0.0114	-0.0131	-0.0685	0.0268	0.021	-0.0834	0.015	0.0032	0	0.3684	0.0581	-0.016	0.5944	0.2625	0.5947	101.4768	-23.4316	75.8838
42	0.0117	-0.0069	-0.0052	0.0044	0.0046	0.0052	0.0051	0.0252	0.0078	-0.0494	0.0985	0.0744	0.0447	-0.018	-0.0001	0.505	-0.0323	-0.0944	0.7743	-0.1569	0.4285	1.6299	-16.7136	7.9512
43	0.0043	-0.0034	0.0011	0.0024	-0.0022	0.0011	0.009	0.0444	0.0082	-0.0343	0.041	-0.0301	-0.0206	0.0186	0	0.642	0.1463	0.1387	0.8586	0.1546	0.5654	86.7412	-4.9327	-22.1562
44	0.0336	0.0489	-0.016	0.0485	-0.0309	0.0088	-0.069	0.115	0.0795	-0.1371	0.0063	-0.2085	-0.1356	0.0734	0.0001	0.5398	-0.1216	-0.0836	0.4593	0.1401	0.5055	73.0988	61.2081	31.9488
45	0.0295	0.0042	0.0329	0.0238	-0.0179	0.0393	-0.0184	-0.0566	0.0158	-0.0442	0.0689	-0.1153	-0.1069	0.0732	-0.0037	0.3734	0.1411	0.2552	0.6631	0.1628	0.585	124.7335	-13.4808	1.7069
46	0.0755	-0.007	0.0435	0.0282	0.0091	0.0546	0.0153	0.0275	0.0191	-0.0066	-0.0181	-0.0254	0.0182	-0.0117	0	1.1011	-0.0475	0.027	1.0062	0.0734	1.0298	137.9361	-6.74	115.5409
47	0.0205	-0.0022	-0.0696	-0.002	0.0703	0.077	-0.0205	-0.0371	-0.0987	0.1332	-0.1005	0.0127	0.1138	-0.0916	-0.0018	0.7661	0.0275	0.0707	0.7368	0.2064	0.4429	48.9006	40.6854	-47.6821
48	0.092	0.0252	-0.0114	0.1215	-0.0189	0.1502	-0.0371	0.0537	0.0207	-0.0235	0.024	0.0311	-0.013	-0.1069	0.0008	0.1912	-0.0174	-0.0554	0.2519	0.0187	0.3104	197.2644	-2.6422	175.3908
49	0.0131	0.0224	0.0809	0.0191	0.0209	0.0852	-0.0673	0.0762	0.0335	-0.0274	0.162	-0.4085	-0.1039	0.136	-0.0279	0.3862	-0.1058	-0.0709	0.2341	-0.099	0.6348	264.7768	57.2696	186.6266
50	0.1645	-0.019	0.1185	0.1282	-0.0754	0.1283	-0.1102	0.0251	0.0121	-0.0869	0.1185	0.2221	0.0206	-0.2295	0.0208	0.2565	-0.0123	-0.1387	0.7197	0.118	0.5885	143.6018	-0.5194	152.6461
51	0.0011	-0.0032	-0.0043	0.0714	-0.0428	0.0041	-0.0304	0.0684	0.049	0.0751	-0.0646	-0.0066	-0.161	-0.1843	-0.001	0.3286	0.1107	0.0592	0.4694	-0.0235	0.4313	151.8556	6.9859	218.9109
52	0.1844	0.0654	0.0153	0.0947	0.0399	0.132	-0.0828	0.1107	-0.0681	-0.0146	0.0848	-0.0349	0.1373	-0.0667	0.0097	0.3548	-0.0511	0.0707	0.1395	-0.0513	0.386	225.9738	-65.7877	174.5722
53	0.0574	0.0751	0.0187	0.1908	0.0381	0.0366	0.0062	0.0513	0.0336	-0.1393	0.0261	0.0717	-0.0212	-0.0138	0.0661	0.4151	0.0041	-0.1269	0.4518	0.1261	0.3746	198.0828	26.4899	120.8663
54	0.0378	0.1159	0.0273	0.1441	0.0556	0.1461	-0.112	0.1379	0.0305	-0.0854	0.1071	-0.1551	-0.1837	0.0736	0.0007	0.624	-0.1418	-0.1597	0.2951	-0.002	0.5888	255.1932	84.9731	187.6233
55	0.0555	0.082	0.0259	0.1087	0.0339	0.0041	-0.088	0.2332	0.0116	-0.0414	0.0133	-0.02	-0.1128	-0.0216	0.0001	0.478	-0.1021	0.1246	0.5336	-0.0348	0.3624	253.1156	-16.3464	101.4587
56	0.0143	-0.0118	-0.015	0.0011	0.0026	0.091	0.0679	0.066	0.0365	-0.1201	0.0674	-0.0947	0.1152	-0.0322	0.0114	0.3303	-0.1091	0.0048	0.2233	0.0509	0.449	256.0912	25.3974	133.732
57	0.0944	0.0739	0.0039	0.146	-0.0573	0.0274	0.0172	-0.0237	0.1365	0.1039	-0.0724	0.1375	-0.1654	-0.2201	0.0051	0.6226	0.3608	-0.0141	0.8435	0.0056	1.145	117.8907	48.662	192.0082
58	0.0101	0.0327	0.0459	0.0272	0.0438	0.0606	-0.0264	-0.0032	-0.1163	-0.0357	0.0668	-0.1243	0.095	0.1318	-0	0.3246	0.033	0.0026	0.3452	0.0063	0.5865	277.4658	-44.0402	184.0681
59	0.0845	-0.0484	0.0517	0.0787	0.0403	0.1769	0.1227	-0.0817	-0.0888	0.227	0.0492	-0.4193	0.0872	0.0399	0.3096	0.4841	0.0672	-0.5986	0.2979	-0.0738	0.8343	263.9577	16.8629	197.7865
60	0.0368	-0.0112	0.0497	0.0614	-0.0038	0.0795	0.0884	-0.1608	0.0549	0.1215	0.0609	-0.0312	0.2022	-0.0189	0.0033	0.6229	-0	-0.1301	0.278	-0.0874	0.5371	255.7463	69.6324	224.0799
61	0.057	-0.0334	-0.0017	0.0536	0.0098	0.0046	-0.0213	0.1822	0.1694	-0.0287	0.0554	0.1238	-0.1573	-0.1053	0.275	0.4208	0.1684	-0.1886	0.5464	0.1655	0.5758	165.5203	59.9378	146.307
62	0.0452	-0.0179	-0.0004	0.0517	0.0075	0.0785	-0.1549	0.107	0.0169	-0.0884	0.1288	-0.0111	0.2284	0.0953	0.0012	0.2782	-0.089	0.1282	0.4056	-0.0514	0.4701	283.2615	-23.9989	136.4656
63	0.0916	0.0075	-0.0501	0.071	0.0323	0.0184	-0.0194	-0.0311	0.1186	-0.0074	-0.0216	0.199	-0.0808	-0.1849	-0.0196	0.3554	0.2406	-0.127	0.6066	0.0466	0.6054	148.9762	37.7289	175.4744
64	0.092	-0.0551	-0.118	0.0111	0.0829	0.3467	-0.0695	-0.0345	-0.0541	0.0504	-0.0019	0.0708	0.0791	0.1093	-0.0227	0.207	-0.0263	0.069	0.1436	-0.0674	0.3468	254.3263	-37.0154	150.3858
65	0.0834	0.0084	0.1564	0.3626	-0.0571	0.2594	-0.1299	0.2491	0.1168	-0.3869	0.0451	-0.1741	-0.0354	0.068	-0.0569	0.4305	-0.151	0.0035	0.3765	-0.0319	0.3547	196.8846	-39.5587	136.8901
66	-0.0106	0.0204	-0.0215	0.1011	-0.0611	0.0282	-0.0795	0.1451	0.1757	-0.0948	-0.0274	0.1134	-0.1243	0.029	-0.0048	0.5806	-0.0161	-0.1308	0.3813	0.137	0.4489	209.8159	49.7655	132.9352
67	0.0434	0.1136	-0.0327	0.025	-0.0429	0.0117	0.0775	-0.0716	-0.0235	0.0065	0.0364	0.1337	0.0815	-0.031	0.0128	0.4195	-0.1134	0.0108	0.6157	0.1043	0.6069	147.236	-56.3187	221.0911
68	0.1228	0.0239	-0.1074	0.0358	-0.0031	0.1127	0.0126	-0.0737	-0.0474	0.0313	-0.0263	-0.1031	-0.0497	0.1217	0	0.2936	-0.0298	-0.034	0.3411	-0.0284	0.4903	277.4185	4.4057	158.0649
69	0.0538	-0.0652	-0.0273	0.0349	0.0108	0.1497	0.0152	0.0043	0.0516	-0.0595	0.0034	-0.1585	-0.1588	0.0288	-0.0018	0.3734	-0.105	-0.0735	0.2025	-0.0038	0.4879	248.7413	48.4731	165.0113
70	0.0125	-0.0092	0.0023	0.0211	0.004	0.0067	0.1415	0.0179	-0.1274	0.0249	0.0569	0.0672	-0.0038	-0.0042	0.0006	0.6995	-0.4177	-0.1705	0.8454	0.1136	1.067	144.047	-84.8564	187.394
71	0.0597	0.019	-0.0024	0.0426	-0.0197	0.0108	-0.1027	-0.0795	-0.0991	0.1158	0.0334	0.0557	0.0767	0.0503	-0.0011	0.3993	-0.0219	-0.0345	0.1691	0.0462	0.341	221.4526	-51.093	215.7014
72	-0.008	-0.0192	0.0081	0.0175	-0.0282	0.0172	-0.1203	0.0071	0.0649	0.0378	0.0165	0.1364	0.0495	-0.0126	-0.0037	0.6058	-0.0376	0.0657	0.1844	-0.0126	0.5217	208.9598	-85.2082	212.1241
73	0.0719	0.0395	0.0017	0.0609	-0.0622	0.0767	0.0308	0.0124	0.0901	0.0415	0.0136	0.1512	-0.0649	-0.108	0.0027	0.3394	-0.1095	-0.1475	0.8616	0.0843	0.7597	125.5667	-16.177	164.0979
74	0.0113	-0.0084	0.0006	0.0515	0.0469	0.0491	-0.1177	-0.0161	-0.0871	-0.0284	0.0655	0.0517	0.0742	-0.2015	-0.2171	0.4747	-0.0799	-0.3697	0.7938	0.1555	0.5533	152.7104	-4.0608	114.2787
75	0.0302	0.0182	0.0614	0.0101	0.0416	0.1531	-0.1346	-0.1655	0.0288	0.0076	-0.0944	0.1211	-0.0605	-0.0698	-0.0103	0.4677	-0.0184	0.1976	1.3843	0.1491	1.1697	99.3807	-18.1976	226.3915
76	0.0724	-0.0252	-0.0493	0.029	0.0523	0.104	-0.1283	0.0697	0.1354	-0.0656	0.0189	0.1086	-0.098	-0.0895	-0.2464	0.442	0.0761	-0.2234	0.3145	0.1884	0.5285	193.2203	55.4779	148.7653
77	0.0552	-0.0151	0.0291	0.0214	0.0116	0.0314	-0.0499	0.0473	0.0827	-0.0015	0.0034	0.067	-0.0938	-0.0751	0.0001	0.5216	-0.118	-0.1199	0.3188	0.1159	0.4185	226.761	53.0314	156.5931
78	0.0155	0.0025	-0.0113	0.0088	0.0005	0.0072	-0.0227	-0.019	0.0386	0.008	-0.1596	-0.0083	-0.0285	-0.132	0.0004	0.5658	-0.0142	0.1823	1.055	0.0099	0.771	122.6631	-18.5359	249.4355
79	0.0334	-0.0004	-0.0202	0.0117	0.006	0.0162	0.0479	-0.0143	0.0218	-0.0329	0.0595	0.0373	0.0013	-0.0336	0.002	0.4228	-0.1906	-0.0379	0.3386	-0.0523	0.4909	182.1113	-70.8723	165.552
80	0.0359	-0.0595	-0.0072	0.0545	0.0242	0.0111	-0.0476	0.1693	-0.0558	-0.0828	0.1147	-0.1026	0.0038	-0.0149	0.0207	0.3003	-0.0739	-0.1042	0.4384	0.0429	0.2539	210.7853	0.1813	121.4768
81	0.027	-0.0225	0.0064	0.0249	0.0154	0.0426	-0.1494	0.1232	0.1429	-0.0993	0.0874	0.073	-0.0089	-0.0544	-0.0025	0.3556	-0.0917	0.0726	0.4324	-0.0205	0.3218	244.8445	-18.9043	126.3671
82	0.0073	-0.0077	-0.0205	0.0187	-0.0319	0.0378	0.0347	-0.0178	0.0261	-0.0253	0.1104	0.0896	-0.0154	-0.1794	0.01	0.3114	-0.0064	0.1036	0.9427	0.0842	0.7839	127.4874	-21.0449	210.2027
83	0.064	-0.0302	0.0562	0.0135	-0.0234	0.1178	-0.0792	0.1065	0.0561	-0.0478	0.125	0.0752	-0.0298	-0.2543	-0.0106	0.4626	-0.0306	-0.2593	0.8833	0.1457	0.7392	128.6744	10.0692	144.3759
84	0.024	0.0114	-0.0132	0.0538	0.0367	0.0159	-0.1139	-0.1758	0.0292	-0.0155	-0.0988	0.1442	-0.1164	0.0109	-0.0059	0.6646	0.2476	-0.1458	0.6368	0.0737	0.8603	153.5802	68.6026	182.562
85	-0.0041	0.0065	0.0306	0.0148	-0.0416	0.101	0.0549	0.1912	-0.0622	0.0278	-0.0306	-0.0598	-0.0484	-0.1771	0.0112	0.3115	0.0036	-0.1646	0.5863	0.0524	0.5573	153.4406	2.7309	162.2397
86	0.0078	0.0068	-0.0036	0.0061	-0.0044	0.0054	-0.1007	-0.0735	-0.0268	0.1008	-0.135	0.0703	0.0479	0.0205	0.0001	0.5665	-0.277	-0.0323	1.1333	0.052	1.1219	110.9335	-52.4235	186.7015
87	0.0154	0.0092	-0.0004	0.0057	-0.0053	0.0057	-0	0.0607	-0.0494	-0.0545	0.0082	0.1327	-0.0038	-0.0103	-0.0001	0.4852	-0.1263	-0.0685	0.7868	-0.0535	0.5727	156.1202	-18.5988	154.9833
88	0.017	0.0013	-0.0181	0.0014	-0.0006	0.0206	0.0547	-0.0929	0.0612	-0.0258	0.0171	-0.0916	0.0092	0.0577	0.0147	0.3684	0.1124	-0.1631	0.2944	0.0414	0.5586	268.4421	-27.9605	198.408
89	0.0021	-0.0002	0.0048	0.0049	-0.0021	0.0055	0.0389	0.0417	0.0021	-0.0023	0.0236	0.0333	-0.0067	-0.0157	-0.0001	0.4677	-0.0762	0.1045	0.3658	0.1265	0.4216	192.7372	-9.1859	247.5688
90	0.0292	-0.0018	0.0141	0.0082	0.0144	0.0304	0.0231	-0.1089	0.2274	0.1747	-0.1319	0.0129	-0.0258	-0.057	-0	0.6148	0.1622	-0.068	0.4234	-0.0514	0.759	204.193	59.252	194.3491
91	0.0093	-0.0036	-0.0028	-0.0019	0.0077	0.0152	-0.0492	0.0891	-0.0291	0.0822	-0.0074	0.189	-0.0688	-0.0785	0.0001	0.2733	-0.0463	0.1045	0.5494	0.0056	0.4065	151.4154	-11.6954	232.8078
92	0.0315	-0.0502	-0.0621	0.0256	0.0196	0.0366	-0.0848	0.032	-0.1126	-0.1007	0.0934	0.0721	-0.042	0.0859	0.0045	0.6572	-0.0145	-0.0506	0.474	0.1007	0.673	244.1112	39.7323	258.7179
93	0.1515	-0.0814	-0.0193	0.3548	-0.1713	0.2524	0.0512	0.302	-0.0708	-0.1676	-0.0723	-0.0881	0.0274	-0.0609	0.4405	0.5753	0.2028	0.1008	0.7413	0.0342	0.3094	82.058	-19.0614	-51.7403
94	0.0374	-0.0262	-0.0055	0.0341	-0.0217	0.0181	-0.0104	0.0559	0.0954	-0.021	-0.0343	-0.1317	-0.1383	0.0276	-0.0031	1.259	0.2399	0.5111	1.3419	0.4295	0.9614	126.0273	8.6752	-92.1076
95	0.0033	-0.0139	0.0116	0.0165	-0.0266	0.0389	0.0862	0.079	0.1181	0.0041	-0.1124	-0.1178	-0.1078	0.0737	-0	1.0424	-0.3516	-0.0239	1.1189	0.1381	1.3867	144.5239	28.3347	-24.7469
96	0.0365	-0.0388	0.0432	0.0274	-0.0359	0.057	-0.0833	0.2646	0.0248	-0.2302	0.0415	0.0431	0.0627	0.017	-0	1.116	0.2555	0.1374	1.0698	0.0916	0.617	91.7178	-9.1873	-87.7949
97	0.0168	0.0337	0.0005	0.0588	0.0027	0.0305	0.0092	0.0574	0.0028	-0.0936	0.0192	-0.076	0.0174	-0.1279	0	1.0789	0.2744	0.0543	1.3183	0.2481	0.6418	60.8502	29.6631	-91.2258
98	0.0437	-0.0091	-0.0302	0.039	-0.0259	0.0331	-0.1062	0.1415	-0.1639	-0.1813	0.0668	-0.0409	0.1578	-0.0361	0.0001	1.1791	0.0861	0.0962	1.0031	-0.3226	0.7613	82.8872	-80.4515	-67.7032
99	0.0211	0.0191	0.0083	0.0469	0.046	0.0361	0.0459	-0.0609	0.123	0.0535	0.0413	0.0302	-0.085	0.0988	-0.0001	1.3519	0.0913	-0.0098	1.0154	0.3518	1.3443	101.6098	77.3743	-61.4069
100	0.0553	0.0013	-0.0033	0.0762	-0.0728	0.0358	0.2276	-0.0301	-0.0702	0.042	-0.0368	0.1337	0.0561	-0.0254	0.0012	0.9269	-0.1661	0.0543	0.6599	-0.2401	0.9043	52.929	-97.4149	-21.9347
101	0.0193	0.0339	0.0253	0.0348	0.043	0.043	0.0412	0.013	-0.0146	0.0389	-0.0906	-0.0122	0.0479	-0.0407	0.0262	0.8808	0.4065	0.5293	1.3354	0.1016	0.974	149.8532	-47.3483	-75.075
102	0.02	0.0101	0.0218	0.0361	0.0139	0.0096	-0.0652	-0.0212	-0.2081	-0.0457	0.0136	-0.0479	0.2248	0.0855	0	0.8799	0.1743	0.1677	0.6247	-0.0398	0.8711	98.6179	-88.2294	-16.1892
103	0.013	-0.0205	-0.0175	0.0467	-0.0113	0.0338	-0.1072	0.1376	-0.0979	-0.2103	-0.0536	0.0735	-0.0275	-0.1514	0.0001	1.4537	0.1479	-0.0664	1.3238	-0.0989	0.6926	62.8858	-41.0403	-87.6818
104	0.0155	0.008	-0.0019	-0.0003	0.0005	0.0118	-0.0755	0.0244	-0.0758	0.0128	-0.0164	0.1463	-0.0526	-0.055	0.0001	1.0353	0.0653	0.0509	1.2618	0.2084	0.8933	27.2889	33.6527	-83.3397
105	0.017	-0.026	-0.0133	0.0247	0.0272	0.0446	0.0999	0.0006	-0.1156	-0.0058	-0.0843	-0.1725	0.057	0.1159	0	0.7591	0.1267	0.1447	0.8369	-0.0779	0.8832	111.7882	-66.2952	-35.1618
106	0.0024	0.0057	0.0059	0.0046	0.0061	0.006	-0.0502	0.0228	-0.0451	-0.0359	-0.0002	-0.0304	0.0226	0.0218	0	1.6774	-0.0711	-0.1944	1.8638	-0.0362	1.7048	-5.6503	26.0097	-81.1096
107	0.0177	-0.0246	-0.0062	0.0205	0.004	0.0026	0.0251	-0.019	-0.1731	-0.0065	-0.038	-0.1809	0.0662	-0.012	0	0.5936	0.0948	0.2487	0.9383	0.1484	0.836	139.5798	-29.3967	-39.0904
108	-0.0041	-0.0017	0.0033	0.0036	-0.0043	0.0128	-0.0223	-0.0144	0.0881	0.0298	-0.0619	-0.1448	-0.0512	0.0608	0.0001	1.0145	-0.2547	0.0097	0.4838	0.2375	0.9231	121.277	40.1117	-26.3301
109	0.0002	-0.0012	-0.0046	0.0072	0.007	0.0186	0.0541	0.0094	0.0034	-0.0142	0.0041	0.0843	0.0534	0.1221	0	1.2	0.3957	0.2066	1.0964	0.2532	0.7191	28.8518	70.5047	-42.8808
110	0.0214	0.0125	0.0135	0.0257	0.0279	0.0232	0.0839	-0.0158	0.1075	-0.0312	0.0307	-0.1471	0.053	-0.0142	-0.0001	1.0654	0.2074	0.1223	1.051	0.4728	1.1519	87.7751	77.5882	-85.3603
111	0.0152	-0.0043	0.0017	-0.0044	-0.015	0.0074	-0.0563	-0.0667	0.0612	-0.0602	0.0135	-0.0286	-0.1458	-0.0346	0.0001	0.9296	0.2144	0.2164	0.9128	0.3328	0.8409	114.5208	30.7619	-95.3309
112	0.0037	-0.0125	-0.0109	0.0152	0.008	-0.0026	-0.0487	-0.1771	0.1677	0.0391	0.0428	-0.0934	-0.0712	-0.0366	-0	1.2054	0.2833	0.1924	0.8954	0.1871	0.761	56.3257	90.5355	-41.0363
113	0.0054	-0.0243	-0.0162	0.0579	0.0184	0.0048	0.0471	0.0582	0.104	0.0569	0.0132	-0.0168	0.0226	-0.017	0.0142	1.3422	0.3628	0.2412	0.7708	0.4942	1.0781	64.1716	95.5313	-73.5699
114	0.036	-0.0099	0.0242	-0.0008	-0.0055	0.0119	-0.1137	-0.0374	-0.087	-0.0482	-0.0866	0.0497	0.0196	0.0498	-0.0067	0.9932	0.256	0.0105	1.2028	0.4193	0.6399	59.3389	56.156	-99.4026
115	0.0141	-0.0136	-0.0245	0.01	0.0168	0.0347	-0.007	0.0547	-0.0309	-0.0415	-0.0128	-0.0184	0.0639	-0.0794	0	1.2339	0.173	0.4835	1.3399	0.1356	0.9158	117.5546	-11.5534	-93.6964
116	0.0223	-0.0225	-0.0006	0.0276	-0.0113	0.034	-0.0483	0.1189	0.0323	-0.1253	-0.0051	-0.1323	0.1102	0.0307	-0.0016	0.9807	0.2368	0.3749	1.132	0.0842	0.6046	114.5789	-32.6173	-72.8881
117	0.022	0.0264	-0.0283	0.0195	-0.0248	0.0185	-0.0207	0.0751	0.0627	-0.2766	0.0639	0.0309	0.0318	-0.0711	-0.0001	0.7417	0.0768	0.072	0.7869	0.0832	0.6499	64.1473	-20.8524	-44.9855
118	0.0345	-0.0243	0.007	0.0187	0.0123	0.021	0.0017	0.0925	-0.163	-0.1687	0.0932	0.137	-0.0014	-0.0018	-0	1.1122	-0.0579	-0.0653	1.1781	-0.2237	0.7496	44.7682	-76.3489	-67.8049
119	-0.0016	0.0009	0.0014	0.0008	0	0.0006	0.0264	-0.036	0.0114	-0.0333	0.008	-0.0229	0.0142	-0.0061	0	1.5865	0.0489	0.0066	1.2565	0.1472	1.6148	90.4101	102.3792	-50.2814
120	0.0043	-0.0006	-0.0024	0.009	-0.0165	0.0285	0.0589	0.0061	0.0916	-0.005	0.0632	-0.0858	-0.0664	0.0741	-0.0002	0.9632	-0.0302	0.1661	0.8732	0.263	0.7936	117.5102	23.4868	-43.4077
121	0.0188	-0.0151	-0.0207	0.0061	0.0092	0.0199	-0.0043	-0.0133	-0.0851	0.0589	-0.0692	-0.1403	0.0017	-0.0023	0.0001	0.7767	0.0258	0.324	1.1159	0.1642	0.9752	152.0061	-16.7339	-57.6358
122	0.0032	-0.0035	0	0.0046	-0.0043	0.0021	-0.0314	-0.0016	0.0182	0.031	-0.0029	-0.0118	-0.0285	-0.05	0.0001	1.0943	0.0765	-0.1079	1.2481	0.2688	1.4289	117.7454	57.2619	-58.5056
123	0.0032	-0.0044	0.0025	0.0015	-0.0056	0.0109	-0.0094	0.0292	-0.0259	-0.0643	-0.0113	-0.0219	0.0792	-0.0092	0.0001	0.9282	0.1485	0.0909	1.0949	0.2327	0.5997	48.7082	48.1181	-73.0759
124	0.0148	-0.0065	-0.0024	0.0081	-0.0065	0.0099	0.0077	0.0925	-0.0343	-0.1083	0.0396	0.0603	0.0529	0.0309	-0	0.8635	0.162	0.0133	0.9742	-0.0075	0.8795	49.7769	-12.261	-73.6103
125	0.0052	-0.0044	0.0058	-0.0029	-0.0041	-0.003	-0.0081	0.0417	0.0574	-0.0667	0.0517	0.0675	-0.0223	-0.0202	0	1.1673	0.0334	0.0171	1.3945	0.0977	1.2606	2.49	43.4078	-60.7056
126	0.037	-0.0256	0.0012	0.0082	-0.01	0.0503	0.0942	0.0502	0.0582	-0.0787	-0.0433	-0.0823	0.0198	0.1075	-0	1.2168	0.2717	0.3609	1.4907	0.636	1.1352	106.3837	58.1436	-117.3143
127	0.0296	0.0085	0.0015	-0.0016	0.0024	0.0114	0.041	-0.1484	0.1748	0.2012	-0.124	0.1185	-0.0362	0.0371	-0.0026	1.375	-0.289	0.4427	0.7889	-0.2298	0.7499	169.107	1.7332	325.086
128	0.0124	0.0028	-0.0043	0.0851	0.0776	0.062	-0.0017	0.0465	0.1355	0.0294	-0.1321	0.1488	-0.1208	-0.2132	0.0001	0.8052	0.1996	0.2395	0.8954	-0.1107	0.9806	137.5539	25.4161	263.284
129	0.0633	-0.0158	0.0221	0.0564	0.0265	0.0662	0.113	-0.3172	0.1003	0.1832	-0.0647	-0.0465	0.0595	-0.0065	-0.0001	1.1693	-0.275	-0.0132	0.6067	0.0208	0.5496	201.0392	-16.7363	312.4985
130	0.0018	-0.0246	0.014	0.0819	-0.0675	0.0419	-0.1738	-0.2319	-0.2453	0.1564	-0.1214	-0.0743	0.213	0.0774	-0.0001	0.9496	0.0303	0.1007	0.7393	0.1353	1.1633	232.3642	23.2652	313.8019
131	0.048	0.026	0.0004	0.0375	0.0407	0.0145	-0.029	-0.087	-0.0767	0.3208	0.0896	0.0755	0.2102	-0.0615	0.0017	1.2151	-0.1546	-0.1077	0.4086	0.1222	0.6056	201.9599	-87.2271	287.7641
132	0.0683	-0.0531	0.0144	0.0495	0.0118	0.0223	0.0258	0.102	0.199	0.0458	0.0724	0.1413	0.0684	-0.1093	-0.0002	0.8726	-0.0972	0.1499	0.7526	0.0037	1.2595	188.3866	70.1361	288.0915
133	0.0783	-0.016	-0.057	0.0995	0.0821	0.2647	0.1174	0.0952	-0.1992	0.2219	-0.004	-0.2672	0.1351	-0.1078	0.0095	0.9179	0.0851	-0.0591	0.3976	0.0308	0.881	221.9657	-102.7779	234.8042
134	0.0579	-0.0449	0.0325	0.0424	-0.0221	0.0259	0.1101	-0.0447	-0.0717	-0.0063	-0.0316	0.1035	0.0281	-0.007	0.0348	1.0125	-0.3857	0.382	0.96	0.0286	0.7693	139.9472	-52.5581	311.2324
135	0.0049	-0.0125	0.0047	0.0485	-0.0008	-0.0009	0.0955	0.096	-0.2132	-0.0087	0.0075	0.1157	0.1347	-0.1021	-0.0001	0.7269	-0.2185	-0.0475	0.48	0.0109	0.7188	175.4314	-91.6331	240.527
136	0.0292	0.0088	-0.0077	0.0313	0.0245	0.0307	-0.0208	-0.103	-0.0039	0.2615	-0.0469	-0.0796	0.1126	0.0523	-0	1.3412	-0.129	-0.2792	0.7161	0.0902	0.5462	227.6118	-49.6277	304.7804
137	0.002	-0.0058	0.0064	0.0088	0.0053	0.0197	0.0976	0.0451	-0.0948	0.0593	0.0195	0.1598	0.1248	-0.0699	-0.0001	0.7332	-0.2114	0.1016	0.4881	0.0178	0.7659	168.4805	-71.0176	262.7943
138	-0.0009	0.0012	0.0005	-0.0015	0.0004	-0.001	0.0039	0.0105	0.0393	0.0788	-0.0081	0.0036	0.0116	0.0176	0	1.7749	0.1182	0.0653	1.9424	0.0435	2.0866	297.7529	20.1674	295.9233
139	0.0106	0.0096	-0.0003	0.03	-0.0008	0.0085	-0.0024	-0.1876	-0.0666	0.1294	-0.0699	0.2117	-0.0029	-0.1146	0.0001	0.6562	-0.1794	0.1806	0.7352	0.0051	0.6785	143.0725	-33.0762	274.387
140	0.0009	0.0046	0.005	0.0222	0.0122	0.0037	-0.0152	0.0502	0.11	-0.1181	-0.0331	-0.0541	-0.198	-0.0063	0	0.9164	0.1645	0.148	0.6415	-0.1274	0.8311	160.6341	37.1566	260.4091
141	0.0058	-0.0044	-0.0179	0.0004	0.0134	0.0398	-0.0601	0.1753	0.071	-0.1138	-0.1552	-0.0635	0.118	0.0619	0.0001	1.0415	-0.218	0.3599	0.808	-0.0838	1.1239	257.5318	60.1009	258.6785
142	0.0152	0.0124	-0.0212	0.0352	-0.0303	0.024	0.0509	-0.0562	0.1532	0.06	0.0511	0.0506	0.0017	-0.0062	-0.0001	0.9946	-0.104	0.2205	0.5963	-0.1449	0.9705	205.6956	71.0705	309.776
143	0.0503	0.0167	0.0128	0.0412	-0.0127	0.0344	-0.1079	-0.0005	-0.0006	0.11	-0.1154	0.0507	-0.0847	-0.0087	-0.0189	1.1189	-0.1669	0.2753	0.8373	-0.2488	0.7641	181.4216	25.6927	326.3171
144	0.0211	0.0343	-0.0182	0.0401	-0.0037	0.0257	-0.1149	0.2892	0.0713	-0.1605	0.0362	0.0254	0.1506	-0.099	0.0001	0.9684	-0.334	0.0479	0.8876	0.1358	0.9551	230.4158	81.2345	260.5031
145	0.0064	0.0118	-0.0013	0.0182	0.0139	0.0028	0.0738	-0.0179	0.1803	0.0969	0.1219	0.0816	-0.0609	0.0062	-0.0001	1.0123	-0.1903	0.0808	0.622	-0.1342	1.142	227.5128	87.8678	293.7527
146	0.0106	0.0127	-0.0045	0.0084	-0.0082	0.0041	-0.0114	-0.0235	0.0354	0.0398	-0.0378	0.0027	-0.0364	-0.0196	0	1.2291	0.2372	0.2287	1.2601	0.216	1.4194	264.52	27.4444	301.8145
147	0.0254	-0.0336	0.0012	0.0228	0.004	-0.0022	-0.1137	0.0051	-0.1935	-0.0282	-0.0618	-0.037	0.0956	-0.0574	-0	0.955	-0.1381	-0.1456	0.8055	-0.1234	0.8018	236.143	50.4202	321.1676
148	0.0166	0.0057	0.0135	0.0152	0.0095	0.0322	-0.0263	-0.139	0.1024	0.0975	-0.0957	0.1346	0.0786	-0.0336	-0.0025	1.2132	-0.2002	0.3162	0.8625	0.0059	0.6608	176.8025	-18.6079	323.6533
149	0.0122	0.0156	0.0098	0.0238	-0.0107	0.0199	0.0379	-0.1459	-0.0598	0.1117	-0.0759	0.1129	-0.0107	0.1295	0	0.9453	-0.1719	0.2285	0.5871	0.0383	0.4528	174.5415	-38.5561	301.8522
150	0.0158	0.0157	0.0262	0.074	0.0579	0.0463	0.0293	-0.0441	0.0035	0.1279	-0.0277	-0.1534	0.0035	-0.0049	-0	0.5628	-0.1017	0.0065	0.3936	-0.0154	0.5275	218.4124	-27.093	264.4206
151	0.0261	-0.0493	0.0207	0.0344	-0.0094	0.0167	0.0495	-0.0243	-0.1364	0.3315	0.0527	-0.1815	0.0636	0.0527	0	1.016	0.0388	-0.3626	0.4711	0.0982	0.9397	239.3005	-84.5636	280.0035
152	0.01	0.0077	0.0062	0.0061	0.0016	0.0016	0.0002	0.0559	-0.0299	-0.0468	-0.0262	0.0416	-0.0607	-0.0484	-0	1.014	-0.0669	0.1575	0.773	0.1979	1.4881	198.5611	94.3171	274.162
153	0.0121	-0.0114	0.0302	0.0083	-0.0086	0.0418	0.1163	-0.0252	-0.0082	0.0262	0.0408	0.1015	-0.1723	-0.025	-0	0.9085	0.0086	0.1635	0.6594	-0.0945	0.5271	167.632	18.8244	275.9106
154	0.0124	0.0126	-0.0099	0.0135	-0.0067	0.0092	0.0449	-0.0319	-0.0732	0.1175	-0.0922	0.1055	-0.0572	0.0076	0	0.8074	-0.0596	0.4114	0.8977	0.0206	0.7833	135.2588	-20.8823	295.4198
155	0.0062	0.0045	-0.0062	0.002	-0.0023	0.0025	-0.0173	-0.0151	0.0374	-0.0597	-0.0001	0.0576	0.0081	-0.066	-0	0.8892	-0.1631	0.2779	1.0505	0.0973	1.2414	171.767	50.241	288.5394
156	0.004	0.005	0.0055	0.0131	0.0072	0.0115	0.0484	0.0019	0.0163	-0.055	-0.0244	0.0025	0.0258	0.0575	-0	0.8811	-0.171	0.0219	0.7916	-0.1019	1.1564	242.0869	40.5223	293.141
157	0.0234	0.0014	-0.0023	0.0237	0.0163	0.0079	0.0977	0.0652	0.119	0.077	0.0217	-0.1152	-0.0555	-0.0004	-0.0001	0.7739	-0.2132	-0.1578	0.6894	0.0158	0.7554	239.0251	-20.3356	289.929
158	-0.0005	0.0011	-0	0.0044	-0.0026	0.0006	-0.0046	-0.0554	0.0075	0.0256	0.0058	0.0144	-0.0043	-0.0216	-0	1.7169	0.0862	0.0204	1.7852	0.056	1.812	294.8078	39.816	279.5578
159	0.0028	0.0058	-0.0021	0.0132	0.0014	0.0495	0.0602	-0.1571	0.1825	0.3329	-0.0336	-0.0677	0.004	-0.0835	-0	1.5738	-0.1537	0.3855	1.2116	-0.3975	0.958	191.1688	53.4343	344.4866
160	0.0247	0.0081	0.0061	0.0302	-0.0348	0.0464	0.0972	-0.002	0.0087	0.0178	-0.0222	-0.0243	0.0452	0.1602	-0.0001	1.1311	-0.0052	-0.1112	1.2014	0.2594	1.5576	324.7389	-14.8542	187.6109

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	chain 1	1	3 - 3396
2	X-RAY DIFFRACTION	2	chain 3	3	1 - 121
3	X-RAY DIFFRACTION	3	chain 4	4	1 - 158
4	X-RAY DIFFRACTION	4	chain j	j	2 - 253
5	X-RAY DIFFRACTION	5	chain k	k	2 - 387
6	X-RAY DIFFRACTION	6	chain l	l	2 - 362
7	X-RAY DIFFRACTION	7	chain m	m	2 - 297
8	X-RAY DIFFRACTION	8	chain n	n	2 - 176
9	X-RAY DIFFRACTION	9	chain o	o	23 - 244
10	X-RAY DIFFRACTION	10	chain p	p	24 - 256
11	X-RAY DIFFRACTION	11	chain q	q	1 - 191
12	X-RAY DIFFRACTION	12	chain r	r	2 - 221
13	X-RAY DIFFRACTION	13	chain s	s	6 - 174
14	X-RAY DIFFRACTION	14	chain t	t	2 - 194
15	X-RAY DIFFRACTION	15	chain u	u	3 - 138
16	X-RAY DIFFRACTION	16	chain v	v	2 - 204
17	X-RAY DIFFRACTION	17	chain w	w	3 - 199
18	X-RAY DIFFRACTION	18	chain x	x	2 - 184
19	X-RAY DIFFRACTION	19	chain y	y	2 - 186
20	X-RAY DIFFRACTION	20	chain z	z	2 - 189
21	X-RAY DIFFRACTION	21	chain 0	0	1 - 172
22	X-RAY DIFFRACTION	22	chain 2	2	2 - 160
23	X-RAY DIFFRACTION	23	chain 5	5	9 - 108
24	X-RAY DIFFRACTION	24	chain l2	l2	2 - 137
25	X-RAY DIFFRACTION	25	chain 6	6	1 - 1800
26	X-RAY DIFFRACTION	26	chain 7	7	1 - 98
27	X-RAY DIFFRACTION	27	chain 8	8	22 - 142
28	X-RAY DIFFRACTION	28	chain 9	9	2 - 127
29	X-RAY DIFFRACTION	29	chain AA	AA	2 - 136
30	X-RAY DIFFRACTION	30	chain AB	AB	2 - 149
31	X-RAY DIFFRACTION	31	chain AC	AC	2 - 59
32	X-RAY DIFFRACTION	32	chain AD	AD	9 - 105
33	X-RAY DIFFRACTION	33	chain AE	AE	4 - 112
34	X-RAY DIFFRACTION	34	chain AF	AF	2 - 128
35	X-RAY DIFFRACTION	35	chain AG	AG	2 - 107
36	X-RAY DIFFRACTION	36	chain AH	AH	2 - 113
37	X-RAY DIFFRACTION	37	chain AI	AI	2 - 120
38	X-RAY DIFFRACTION	38	chain AJ	AJ	2 - 100
39	X-RAY DIFFRACTION	39	chain AK	AK	2 - 100
40	X-RAY DIFFRACTION	40	chain AL	AL	2 - 78
41	X-RAY DIFFRACTION	41	chain AM	AM	2 - 51
42	X-RAY DIFFRACTION	42	chain AN	AN	77 - 500
43	X-RAY DIFFRACTION	43	chain AO	AO	1 - 25
44	X-RAY DIFFRACTION	44	chain AP	AP	2 - 501
45	X-RAY DIFFRACTION	45	chain AQ	AQ	2 - 501
46	X-RAY DIFFRACTION	46	chain o1	o1	1 - 1515
47	X-RAY DIFFRACTION	47	chain i	i	9 - 176
48	X-RAY DIFFRACTION	48	chain AR	AR	3 - 3396
49	X-RAY DIFFRACTION	49	chain AS	AS	1 - 121
50	X-RAY DIFFRACTION	50	chain AT	AT	1 - 158
51	X-RAY DIFFRACTION	51	chain CD	CD	2 - 253
52	X-RAY DIFFRACTION	52	chain CE	CE	2 - 387
53	X-RAY DIFFRACTION	53	chain CF	CF	2 - 362
54	X-RAY DIFFRACTION	54	chain CG	CG	2 - 297
55	X-RAY DIFFRACTION	55	chain CH	CH	2 - 176
56	X-RAY DIFFRACTION	56	chain CI	CI	23 - 244
57	X-RAY DIFFRACTION	57	chain CJ	CJ	24 - 256
58	X-RAY DIFFRACTION	58	chain CK	CK	1 - 191
59	X-RAY DIFFRACTION	59	chain CL	CL	2 - 221
60	X-RAY DIFFRACTION	60	chain CM	CM	6 - 174
61	X-RAY DIFFRACTION	61	chain CN	CN	2 - 194
62	X-RAY DIFFRACTION	62	chain CO	CO	3 - 138
63	X-RAY DIFFRACTION	63	chain CP	CP	2 - 204
64	X-RAY DIFFRACTION	64	chain CQ	CQ	3 - 199
65	X-RAY DIFFRACTION	65	chain CR	CR	2 - 184
66	X-RAY DIFFRACTION	66	chain CS	CS	2 - 186
67	X-RAY DIFFRACTION	67	chain CT	CT	2 - 189
68	X-RAY DIFFRACTION	68	chain CU	CU	1 - 172
69	X-RAY DIFFRACTION	69	chain CV	CV	2 - 160
70	X-RAY DIFFRACTION	70	chain CW	CW	9 - 108
71	X-RAY DIFFRACTION	71	chain CX	CX	2 - 137
72	X-RAY DIFFRACTION	72	chain CY	CY	1 - 98
73	X-RAY DIFFRACTION	73	chain CZ	CZ	22 - 142
74	X-RAY DIFFRACTION	74	chain DA	DA	2 - 127
75	X-RAY DIFFRACTION	75	chain DB	DB	2 - 136
76	X-RAY DIFFRACTION	76	chain DC	DC	2 - 149
77	X-RAY DIFFRACTION	77	chain DD	DD	2 - 59
78	X-RAY DIFFRACTION	78	chain DE	DE	9 - 105
79	X-RAY DIFFRACTION	79	chain DF	DF	4 - 112
80	X-RAY DIFFRACTION	80	chain DG	DG	2 - 128
81	X-RAY DIFFRACTION	81	chain DH	DH	2 - 107
82	X-RAY DIFFRACTION	82	chain DI	DI	2 - 113
83	X-RAY DIFFRACTION	83	chain DJ	DJ	2 - 120
84	X-RAY DIFFRACTION	84	chain DK	DK	2 - 100
85	X-RAY DIFFRACTION	85	chain DL	DL	2 - 100
86	X-RAY DIFFRACTION	86	chain DM	DM	2 - 78
87	X-RAY DIFFRACTION	87	chain DN	DN	2 - 51
88	X-RAY DIFFRACTION	88	chain DO	DO	77 - 500
89	X-RAY DIFFRACTION	89	chain DP	DP	1 - 25
90	X-RAY DIFFRACTION	90	chain DQ	DQ	2 - 501
91	X-RAY DIFFRACTION	91	chain DR	DR	2 - 501
92	X-RAY DIFFRACTION	92	chain sM	sM	23 - 174
93	X-RAY DIFFRACTION	93	chain A	A	1 - 1798
94	X-RAY DIFFRACTION	94	chain B	B	2 - 207
95	X-RAY DIFFRACTION	95	chain C	C	20 - 233
96	X-RAY DIFFRACTION	96	chain D	D	34 - 250
97	X-RAY DIFFRACTION	97	chain E	E	3 - 225
98	X-RAY DIFFRACTION	98	chain F	F	2 - 261
99	X-RAY DIFFRACTION	99	chain G	G	20 - 225
100	X-RAY DIFFRACTION	100	chain H	H	1 - 226
101	X-RAY DIFFRACTION	101	chain I	I	4 - 187
102	X-RAY DIFFRACTION	102	chain J	J	2 - 200
103	X-RAY DIFFRACTION	103	chain K	K	2 - 186
104	X-RAY DIFFRACTION	104	chain L	L	1 - 96
105	X-RAY DIFFRACTION	105	chain M	M	2 - 156
106	X-RAY DIFFRACTION	106	chain N	N	20 - 143
107	X-RAY DIFFRACTION	107	chain O	O	2 - 151
108	X-RAY DIFFRACTION	108	chain P	P	11 - 137
109	X-RAY DIFFRACTION	109	chain Q	Q	8 - 131
110	X-RAY DIFFRACTION	110	chain R	R	3 - 143
111	X-RAY DIFFRACTION	111	chain S	S	2 - 126
112	X-RAY DIFFRACTION	112	chain T	T	2 - 146
113	X-RAY DIFFRACTION	113	chain U	U	2 - 144
114	X-RAY DIFFRACTION	114	chain V	V	15 - 121
115	X-RAY DIFFRACTION	115	chain W	W	1 - 87
116	X-RAY DIFFRACTION	116	chain X	X	2 - 130
117	X-RAY DIFFRACTION	117	chain Y	Y	2 - 145
118	X-RAY DIFFRACTION	118	chain Z	Z	2 - 135
119	X-RAY DIFFRACTION	119	chain a	a	36 - 105
120	X-RAY DIFFRACTION	120	chain b	b	2 - 500
121	X-RAY DIFFRACTION	121	chain c	c	2 - 101
122	X-RAY DIFFRACTION	122	chain d	d	5 - 67
123	X-RAY DIFFRACTION	123	chain e	e	4 - 99
124	X-RAY DIFFRACTION	124	chain f	f	2 - 61
125	X-RAY DIFFRACTION	125	chain g	g	82 - 501
126	X-RAY DIFFRACTION	126	chain h	h	2 - 319
127	X-RAY DIFFRACTION	127	chain s0	s0	2 - 207
128	X-RAY DIFFRACTION	128	chain s1	s1	20 - 235
129	X-RAY DIFFRACTION	129	chain s2	s2	34 - 250
130	X-RAY DIFFRACTION	130	chain s3	s3	3 - 225
131	X-RAY DIFFRACTION	131	chain s4	s4	2 - 261
132	X-RAY DIFFRACTION	132	chain s5	s5	20 - 225
133	X-RAY DIFFRACTION	133	chain s6	s6	1 - 218
134	X-RAY DIFFRACTION	134	chain s7	s7	2 - 187
135	X-RAY DIFFRACTION	135	chain s8	s8	2 - 200
136	X-RAY DIFFRACTION	136	chain s9	s9	2 - 186
137	X-RAY DIFFRACTION	137	chain c1	c1	2 - 147
138	X-RAY DIFFRACTION	138	chain c2	c2	20 - 143
139	X-RAY DIFFRACTION	139	chain c3	c3	2 - 151
140	X-RAY DIFFRACTION	140	chain c4	c4	10 - 137
141	X-RAY DIFFRACTION	141	chain c5	c5	4 - 138
142	X-RAY DIFFRACTION	142	chain c6	c6	2 - 143
143	X-RAY DIFFRACTION	143	chain c7	c7	2 - 122
144	X-RAY DIFFRACTION	144	chain c8	c8	2 - 146
145	X-RAY DIFFRACTION	145	chain c9	c9	2 - 144
146	X-RAY DIFFRACTION	146	chain c0	c0	1 - 98
147	X-RAY DIFFRACTION	147	chain d0	d0	12 - 121
148	X-RAY DIFFRACTION	148	chain d1	d1	1 - 87
149	X-RAY DIFFRACTION	149	chain d2	d2	2 - 130
150	X-RAY DIFFRACTION	150	chain d3	d3	2 - 145
151	X-RAY DIFFRACTION	151	chain d4	d4	2 - 135
152	X-RAY DIFFRACTION	152	chain d5	d5	37 - 105
153	X-RAY DIFFRACTION	153	chain d6	d6	2 - 500
154	X-RAY DIFFRACTION	154	chain d7	d7	2 - 1
155	X-RAY DIFFRACTION	155	chain d8	d8	5 - 67
156	X-RAY DIFFRACTION	156	chain d9	d9	4 - 99
157	X-RAY DIFFRACTION	157	chain e0	e0	2 - 63
158	X-RAY DIFFRACTION	158	chain e1	e1	102 - 501
159	X-RAY DIFFRACTION	159	chain sR	sR	2 - 319
160	X-RAY DIFFRACTION	160	chain p0	p0	3 - 221