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- PDB-5kan: Crystal structure of multidonor HV1-18-class broadly neutralizing... -

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基本情報

登録情報
データベース: PDB / ID: 5kan
タイトルCrystal structure of multidonor HV1-18-class broadly neutralizing Influenza A antibody 16.g.07 in complex with A/Hong Kong/1-4-MA21-1/1968 (H3N2) Hemagglutinin
要素
  • (Hemagglutinin ...) x 2
  • 16.g.07 Heavy chain
  • 16.g.07 Light chain
キーワードIMMUNE SYSTEM / Influenza / multidonor / H5 / universal influenza vaccine
機能・相同性
機能・相同性情報


viral budding from plasma membrane / clathrin-dependent endocytosis of virus by host cell / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane / membrane
類似検索 - 分子機能
Hemagglutinin Ectodomain; Chain B - #10 / Hemagglutinin Ectodomain; Chain B / Hemagglutinin (Ha1 Chain); Chain: A; domain 1 / Haemagglutinin, alpha/beta domain, HA1 chain / Haemagglutinin, influenzavirus A / Haemagglutinin, HA1 chain, alpha/beta domain superfamily / Haemagglutinin / Haemagglutinin, influenzavirus A/B / Viral capsid/haemagglutinin protein / Immunoglobulins ...Hemagglutinin Ectodomain; Chain B - #10 / Hemagglutinin Ectodomain; Chain B / Hemagglutinin (Ha1 Chain); Chain: A; domain 1 / Haemagglutinin, alpha/beta domain, HA1 chain / Haemagglutinin, influenzavirus A / Haemagglutinin, HA1 chain, alpha/beta domain superfamily / Haemagglutinin / Haemagglutinin, influenzavirus A/B / Viral capsid/haemagglutinin protein / Immunoglobulins / Alpha-Beta Complex / Immunoglobulin-like / Sandwich / Mainly Beta / Alpha Beta
類似検索 - ドメイン・相同性
生物種Influenza A virus (A型インフルエンザウイルス)
Homo sapiens (ヒト)
手法X線回折 / シンクロトロン / 分子置換 / 解像度: 2.785 Å
データ登録者Joyce, M.G. / Thomas, P.V. / Wheatley, A.K. / McDermott, A.B. / Mascola, J.R. / Kwong, P.D.
引用ジャーナル: Cell(Cambridge,Mass.) / : 2016
タイトル: Vaccine-Induced Antibodies that Neutralize Group 1 and Group 2 Influenza A Viruses.
著者: Joyce, M.G. / Wheatley, A.K. / Thomas, P.V. / Chuang, G.Y. / Soto, C. / Bailer, R.T. / Druz, A. / Georgiev, I.S. / Gillespie, R.A. / Kanekiyo, M. / Kong, W.P. / Leung, K. / Narpala, S.N. / ...著者: Joyce, M.G. / Wheatley, A.K. / Thomas, P.V. / Chuang, G.Y. / Soto, C. / Bailer, R.T. / Druz, A. / Georgiev, I.S. / Gillespie, R.A. / Kanekiyo, M. / Kong, W.P. / Leung, K. / Narpala, S.N. / Prabhakaran, M.S. / Yang, E.S. / Zhang, B. / Zhang, Y. / Asokan, M. / Boyington, J.C. / Bylund, T. / Darko, S. / Lees, C.R. / Ransier, A. / Shen, C.H. / Wang, L. / Whittle, J.R. / Wu, X. / Yassine, H.M. / Santos, C. / Matsuoka, Y. / Tsybovsky, Y. / Baxa, U. / Mullikin, J.C. / Subbarao, K. / Douek, D.C. / Graham, B.S. / Koup, R.A. / Ledgerwood, J.E. / Roederer, M. / Shapiro, L. / Kwong, P.D. / Mascola, J.R. / McDermott, A.B.
履歴
登録2016年6月1日登録サイト: RCSB / 処理サイト: RCSB
改定 1.02016年11月30日Provider: repository / タイプ: Initial release
改定 1.12020年7月29日Group: Advisory / Data collection ...Advisory / Data collection / Database references / Derived calculations / Structure summary
カテゴリ: chem_comp / citation ...chem_comp / citation / entity / pdbx_chem_comp_identifier / pdbx_entity_nonpoly / pdbx_unobs_or_zero_occ_atoms / struct_conn / struct_site / struct_site_gen
Item: _chem_comp.name / _chem_comp.type ..._chem_comp.name / _chem_comp.type / _citation.journal_id_CSD / _entity.pdbx_description / _pdbx_entity_nonpoly.name / _struct_conn.pdbx_role
解説: Carbohydrate remediation / Provider: repository / タイプ: Remediation
改定 1.22023年9月27日Group: Advisory / Data collection ...Advisory / Data collection / Database references / Refinement description / Structure summary
カテゴリ: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_unobs_or_zero_occ_atoms / struct_ncs_dom_lim
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI ..._chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id
改定 1.32024年11月20日Group: Structure summary
カテゴリ: pdbx_entry_details / pdbx_modification_feature

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構造の表示

構造ビューア分子:
MolmilJmol/JSmol

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集合体

登録構造単位
A: Hemagglutinin HA1
B: Hemagglutinin HA2
C: Hemagglutinin HA1
D: Hemagglutinin HA2
E: Hemagglutinin HA1
F: Hemagglutinin HA2
G: 16.g.07 Heavy chain
H: 16.g.07 Heavy chain
I: 16.g.07 Light chain
J: 16.g.07 Heavy chain
K: 16.g.07 Light chain
L: 16.g.07 Light chain
ヘテロ分子


分子量 (理論値)分子数
合計 (水以外)314,04129
ポリマ-310,28012
非ポリマー3,76117
2,666148
1


  • 登録構造と同一
  • 登録者・ソフトウェアが定義した集合体
タイプ名称対称操作
identity operation1_555x,y,z1
Buried area52490 Å2
ΔGint-176 kcal/mol
Surface area117220 Å2
手法PISA
単位格子
Length a, b, c (Å)128.847, 147.562, 209.786
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
非結晶学的対称性 (NCS)NCSドメイン:
IDEns-ID詳細
11chain A
21chain C
31chain E
12chain B
22chain D
32chain F
13chain G
23chain H
33chain J
14chain I
24chain K
34chain L

NCSドメイン領域:

Component-ID: 1

Dom-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11ASNASNLYSLYSchain AAA8 - 3261 - 319
21ASNASNLYSLYSchain CCC8 - 3261 - 319
31ASNASNLYSLYSchain EEE8 - 3261 - 319
12GLYGLYVALVALchain BBB4 - 1761 - 173
22GLYGLYLYSLYSchain DDD4 - 1741 - 171
32GLYGLYILEILEchain FFF4 - 1731 - 170
13GLNGLNCYSCYSchain GGG1 - 2311 - 231
23GLNGLNCYSCYSchain HHH1 - 2311 - 231
33GLNGLNCYSCYSchain JJJ1 - 2311 - 231
14ASPASPCYSCYSchain III1 - 2141 - 214
24ASPASPCYSCYSchain KKK1 - 2141 - 214
34ASPASPCYSCYSchain LLL1 - 2141 - 214

NCSアンサンブル:
ID
1
2
3
4

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要素

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Hemagglutinin ... , 2種, 6分子 ACEBDF

#1: タンパク質 Hemagglutinin HA1


分子量: 35154.539 Da / 分子数: 3 / 由来タイプ: 組換発現
由来: (組換発現) Influenza A virus (strain A/Hong Kong/1/1968 H3N2) (A型インフルエンザウイルス)
: A/Hong Kong/1/1968 H3N2 / 遺伝子: HA / 発現宿主: Homo sapiens (ヒト) / 参照: UniProt: Q91MA7
#2: タンパク質 Hemagglutinin HA2


分子量: 19980.070 Da / 分子数: 3 / 由来タイプ: 組換発現
由来: (組換発現) Influenza A virus (strain A/Hong Kong/1/1968 H3N2) (A型インフルエンザウイルス)
: A/Hong Kong/1/1968 H3N2 / 遺伝子: HA / 発現宿主: Homo sapiens (ヒト) / 参照: UniProt: Q91MA7

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抗体 , 2種, 6分子 GHJIKL

#3: 抗体 16.g.07 Heavy chain


分子量: 24957.084 Da / 分子数: 3 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 発現宿主: Homo sapiens (ヒト)
#4: 抗体 16.g.07 Light chain


分子量: 23335.080 Da / 分子数: 3 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 発現宿主: Homo sapiens (ヒト)

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/ 非ポリマー , 2種, 165分子

#5: 糖
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE / N-アセチル-β-D-グルコサミン


タイプ: D-saccharide, beta linking / 分子量: 221.208 Da / 分子数: 17 / 由来タイプ: 組換発現 / : C8H15NO6
識別子タイププログラム
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0
#6: 水 ChemComp-HOH / water


分子量: 18.015 Da / 分子数: 148 / 由来タイプ: 天然 / : H2O

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詳細

Has protein modificationY

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実験情報

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実験

実験手法: X線回折 / 使用した結晶の数: 1

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試料調製

結晶マシュー密度: 3.21 Å3/Da / 溶媒含有率: 61.73 %
結晶化温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 6
詳細: 0.010M Tris(2-carboxyethyl)phosphine HCl (TCEP) , 0.09 M 2-(N-morpholino)ethanesulfonic acid (MES), pH 6.0, 12% (w/v) PEG-6000

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データ収集

回折平均測定温度: 100 K
放射光源由来: シンクロトロン / サイト: APS / ビームライン: 22-ID / 波長: 1 Å
検出器タイプ: MARMOSAIC 300 mm CCD / 検出器: CCD / 日付: 2015年4月9日
放射プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長波長: 1 Å / 相対比: 1
反射解像度: 2.78→50 Å / Num. obs: 83716 / % possible obs: 83.2 % / 冗長度: 4.1 % / Net I/σ(I): 14.05

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解析

ソフトウェア
名称バージョン分類
PHENIX1.9_1692精密化
PDB_EXTRACT3.2データ抽出
HKL-2000データ削減
HKL-2000データスケーリング
PHASER位相決定
精密化構造決定の手法: 分子置換
開始モデル: 4lss, 5k9q
解像度: 2.785→40.673 Å / SU ML: 0.29 / 交差検証法: FREE R-VALUE / σ(F): 1.34 / 位相誤差: 26.46
Rfactor反射数%反射
Rfree0.2427 4106 4.91 %
Rwork0.2176 --
obs0.2189 83699 83.25 %
溶媒の処理減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å
原子変位パラメータBiso max: 241 Å2 / Biso mean: 71.5849 Å2 / Biso min: 16.15 Å2
精密化ステップサイクル: final / 解像度: 2.785→40.673 Å
タンパク質核酸リガンド溶媒全体
原子数21758 0 238 148 22144
Biso mean--94.69 45.34 -
残基数----2806
拘束条件
Refine-IDタイプDev ideal
X-RAY DIFFRACTIONf_bond_d0.00422517
X-RAY DIFFRACTIONf_angle_d0.97330567
X-RAY DIFFRACTIONf_chiral_restr0.0393446
X-RAY DIFFRACTIONf_plane_restr0.0033947
X-RAY DIFFRACTIONf_dihedral_angle_d13.7958169
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDRmsタイプ
11A4495X-RAY DIFFRACTION8.279TORSIONAL
12C4495X-RAY DIFFRACTION8.279TORSIONAL
13E4495X-RAY DIFFRACTION8.279TORSIONAL
21B2501X-RAY DIFFRACTION8.279TORSIONAL
22D2501X-RAY DIFFRACTION8.279TORSIONAL
23F2501X-RAY DIFFRACTION8.279TORSIONAL
31G3182X-RAY DIFFRACTION8.279TORSIONAL
32H3182X-RAY DIFFRACTION8.279TORSIONAL
33J3182X-RAY DIFFRACTION8.279TORSIONAL
41I2948X-RAY DIFFRACTION8.279TORSIONAL
42K2948X-RAY DIFFRACTION8.279TORSIONAL
43L2948X-RAY DIFFRACTION8.279TORSIONAL
LS精密化 シェル

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 29

解像度 (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.7846-2.81730.2971230.355753455716
2.8173-2.85170.405490.365987192027
2.8517-2.88770.3669450.32571070111533
2.8877-2.92570.3395580.32091367142541
2.9257-2.96580.3821790.32551595167449
2.9658-3.00820.3771080.30091927203559
3.0082-3.0530.32781020.30982233233568
3.053-3.10070.30751200.30262389250974
3.1007-3.15160.30281240.29062637276180
3.1516-3.20590.3171420.28472781292385
3.2059-3.26410.30921550.28782970312591
3.2641-3.32690.30291690.28263077324694
3.3269-3.39480.30071580.25863168332697
3.3948-3.46860.27952080.26193180338898
3.4686-3.54920.27811400.24443255339599
3.5492-3.63790.25491860.23683234342099
3.6379-3.73620.24022100.23732543464100
3.7362-3.84610.23731510.228832923443100
3.8461-3.97010.25751670.227232943461100
3.9701-4.11190.22111900.20232773467100
4.1119-4.27630.20541490.188433263475100
4.2763-4.47070.19411580.169933173475100
4.4707-4.70610.20491660.165432853451100
4.7061-5.00050.1971780.166433203498100
5.0005-5.38570.19441820.173433213503100
5.3857-5.92620.22711710.192933493520100
5.9262-6.78040.19231650.205933763541100
6.7804-8.52960.20921570.2134053562100
8.5296-40.67710.24611960.1913489368599
精密化 TLS

手法: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
16.77591.8276-1.39952.5557-0.59351.1-0.01932.1508-0.154-0.4260.0463-0.292-0.17150.23210.06370.3899-0.01310.02590.62170.02130.343740.66841.862-81.6655
26.08722.7675-0.24972.3926-0.53230.3958-0.24721.1456-0.575-0.47540.28820.3507-0.1746-0.14780.03280.6301-0.0155-0.12190.7174-0.19250.83090.2308-19.0835-84.5247
35.60950.7741-1.13051.91270.39250.76670.07620.2888-1.4043-0.06820.17750.35090.1177-0.1369-0.15710.6524-0.0931-0.24840.6318-0.17231.6651-26.143-35.4354-79.6517
44.60730.0864-0.68743.9181-0.2573.1605-0.01010.0944-0.5750.27950.28150.3998-0.9946-0.3635-0.28860.5634-0.0341-0.06820.71010.04011.4967-37.8816-28.5759-76.0521
55.28082.5748-1.03142.4658-0.87261.29680.1399-0.3265-1.08950.13660.07840.2850.2527-0.1061-0.34540.5749-0.1261-0.19430.65340.07541.6764-25.6051-34.8079-71.0253
64.68640.76680.26691.2936-0.74221.013-0.17090.6937-1.0795-0.28990.00420.20250.31830.01710.09020.5112-0.0891-0.04350.6946-0.28040.79481.7269-18.989-82.8227
74.654-0.2285-2.70610.4725-0.05661.61770.24620.21461.4783-0.086-0.02520.2704-0.1540.0688-0.04690.4197-0.0680.0030.5427-0.00440.537622.0639-0.6848-80.461
86.45591.79044.61956.63392.07554.2555-0.51051.36681.3359-1.21880.7144-0.4895-0.53140.83460.00260.5765-0.05620.0710.70130.05250.57840.70491.4592-82.3214
910.04851.815-4.51581.5689-1.59753.4537-0.13180.422-0.7173-0.04030.0956-0.3513-0.33260.0032-0.15430.42310.0209-0.00920.4306-0.03090.321539.5757-7.7471-77.3661
103.20781.8224-2.50873.1807-0.11192.22570.3373-0.7253-0.32970.563-0.28350.06550.2698-0.11210.37250.63270.0118-0.0020.5757-0.06851.16874.6383-21.1542-71.7265
114.771-2.0492-4.01355.51830.52513.75040.77120.2913-0.1743-1.0517-0.6122-0.11460.276-1.8411-0.04410.54680.0194-0.05890.9772-0.2130.877-15.4538-12.0019-77.6284
122.54670.1748-1.34490.3303-0.12110.55340.338-0.16680.2591-0.0372-0.18210.0743-0.05210.0853-0.00820.3523-0.0182-0.01430.4696-0.05120.430217.3234-4.0959-69.6415
135.5665-0.21651.76154.74471.82117.64-0.2154-0.0259-0.1061-0.02350.2687-0.2533-0.11240.3012-0.03930.2849-0.0540.03780.4532-0.04820.491158.38420.6522-71.4172
144.91481.2242-2.57241.1337-2.55936.05570.13810.12030.2878-0.8641-0.0877-0.4108-0.56970.62540.28631.12540.2718-0.07630.89560.04371.772567.669915.4783-67.1948
153.87640.52910.23510.6145-0.12530.20730.0711-0.4624-0.66440.1515-0.0503-0.11030.01320.1011-0.01450.50640.02760.0160.5051-0.01290.49039.65-18.3457-45.3207
169.82564.17965.33585.97740.60171.92110.4807-0.8396-0.14630.3582-0.16230.8215-0.0167-0.2138-0.08660.51340.02480.05810.48120.06180.5751-21.6949-24.3486-42.5544
175.1550.74441.10373.84370.01292.59420.0201-0.8271-0.35680.34960.06580.8147-0.1723-0.2268-0.12170.42860.10660.07420.56180.20280.6845-38.2956-19.8167-43.3752
182.8146-1.10471.37026.82660.58941.99470.10940.1287-0.199-0.75070.1014-0.2683-0.20050.0911-0.18580.591-0.0544-0.05730.53080.23570.898-39.6513-23.4732-54.295
194.7832-1.66481.24452.7915-0.52792.5721-0.1016-0.5651-0.0213-0.02890.22910.4714-0.43470.0122-0.10030.46860.04840.01270.46950.09770.5383-33.5984-12.8171-46.6126
206.77421.8868-3.13375.1458-2.29971.52920.0652-0.2355-0.08280.19010.1491-0.0665-0.25710.1035-0.14450.4403-0.0303-0.00890.5362-0.05940.3556-0.3537-18.8274-44.657
215.85520.64470.86023.05741.10461.9250.69651.3645-0.80430.6621-0.1553-0.47870.68970.5922-0.03820.47640.092-0.090.57140.00710.452725.4634-16.5351-53.8972
227.47691.87190.45722.0114-0.05380.62620.2879-0.085-0.55550.0579-0.2089-0.41980.23240.2726-0.0880.5150.0675-0.03010.632-0.01220.446938.9854-7.0406-45.6402
234.07263.44082.0482.99251.81640.96320.14640.54561.13670.54590.36710.7345-0.4977-0.58830.00930.7556-0.0490.00461.1897-0.0540.9099-0.6462-7.7007-47.5903
246.49561.65431.04395.07790.81913.69230.24090.7244-1.07660.0906-0.33411.15790.7412-0.7145-0.03611.0646-0.0334-0.13110.84160.02591.0532-13.1558-23.2927-58.683
258.77052.12563.51671.11980.89081.73950.03050.21550.17820.16110.01370.05410.14510.16540.02750.39720.0018-0.01710.4468-0.04770.437417.7801-7.6426-58.3197
267.45771.8728-0.44365.5956-1.38931.1476-0.0709-0.38030.1371-0.0603-0.084-0.5841-0.4360.32740.13470.5102-0.0131-0.12770.6965-0.13260.589557.19164.7194-48.7596
275.6165.6601-2.51854.7718-2.44520.78940.4062-1.73860.96550.0433-0.2294-0.0965-0.04630.1036-0.07990.4368-0.08170.03110.5801-0.27010.703233.341621.719-54.1655
287.05461.17531.31350.16230.29620.7477-0.22810.22650.88740.08410.07740.1666-0.1344-0.03950.15540.47580.0071-0.00760.47590.00620.6344-17.449111.5872-64.8248
295.56971.72560.58793.1208-0.71491.8616-0.0510.6519-0.0045-0.09740.04390.1134-0.0722-0.0566-0.00880.38530.0364-0.03060.5203-0.00460.497-42.24590.1087-76.8416
309.9245-1.4449-1.98546.65920.9123.1691-0.1155-0.3404-0.92350.34120.3780.07110.73920.7178-0.24130.45080.0284-0.0630.49070.0240.6245-43.0667-8.8193-69.5146
315.20660.52060.6752.94080.03761.8577-0.06060.8064-0.5212-0.1552-0.0862-0.2951-0.02780.44860.05940.3830.0445-0.00470.6564-0.11490.5371-34.2236-3.0604-79.5509
323.02580.0971-0.64821.6453-0.0362.06380.0530.23060.7329-0.1694-0.020.1473-0.1372-0.05470.04440.4059-0.0035-0.04120.5083-0.01280.5839-8.828612.5585-64.2001
337.07746.6005-0.64847.75060.54961.05730.8782-0.4735-0.02860.9921-0.3827-0.3412-0.18110.4121-0.12310.4998-0.0559-0.030.5459-0.03540.461116.276413.391-53.6543
347.78642.3530.51013.19270.09560.7214-0.0991-0.47481.12510.28550.1857-0.1648-0.06730.069-0.0390.437-0.0062-0.06720.4748-0.1430.710831.078421.7532-60.9175
352.608-0.15071.51173.07512.3912.41470.05561.1392-0.00580.317-0.13830.5135-0.4975-0.30330.33990.63580.0149-0.01790.56890.08340.7622-11.17371.9974-65.6039
369.44114.91980.50763.68170.51590.4885-0.1065-0.1191-0.561-0.02520.117-0.376-0.00880.0241-0.0630.3829-0.0354-0.02120.4227-0.01690.397414.58773.385-60.9112
377.1645-0.0484-2.37152.8975-1.08083.9554-0.0441-0.13710.9985-0.14630.0249-0.3873-0.0960.51970.11160.4825-0.1032-0.00160.4882-0.15830.789152.486921.6725-62.7686
383.3579-0.39110.47368.83384.15283.36850.1766-0.6022-0.18140.92120.2521-0.99430.31350.6377-0.06210.65040.0288-0.04420.8898-0.00760.563328.82513.0508-18.5288
394.34431.8389-0.27562.83322.88395.6610.1748-0.22650.41650.2065-0.0765-0.1809-0.43370.4396-0.19440.5182-0.03920.00080.56620.03450.466225.713210.0441-25.0185
40-0.1969-0.8888-1.27934.31284.80649.6684-0.0464-0.0533-0.00010.5999-0.0177-0.0147-0.1204-0.0695-0.01750.60920.0002-0.00650.64940.04410.524315.08819.1498-14.2371
415.90092.10530.35794.46840.98455.21130.0283-0.1798-0.01460.61620.1145-0.121-0.40670.3922-0.15410.96140.0229-0.08170.65260.05860.49657.658717.86718.1341
423.5728-1.54761.35976.2005-1.39670.69660.43640.92930.103-0.8494-0.4152-0.60640.10961.2079-0.09590.69130.02770.17010.6149-0.15850.556245.2182-41.1825-82.6568
433.346-2.6462.15997.2741-2.70323.12880.30090.2874-0.7394-0.6871-0.0825-0.4144-0.35990.28480.1280.5467-0.00580.13080.4582-0.03650.656740.3867-28.0072-85.5318
446.06213.0904-1.43172.1296-0.19931.5406-0.3891-0.0773-1.16290.49410.449-0.27070.17310.03130.13180.5453-0.04270.0240.44860.04990.572336.095-40.0071-74.5716
456.55443.1057-0.49512.3440.86162.95170.0371-0.7901-0.18390.6939-0.1573-0.32440.17650.3573-0.08680.53370.05930.02220.48560.02450.445639.0456-28.8852-71.3686
466.7761-0.0274-0.73058.08420.89063.66750.0933-0.03770.06670.43070.0042-0.6476-1.13350.046-0.10470.48990.0071-0.00730.52020.02790.735646.8385-30.7853-77.1096
473.6265-1.6528-0.34548.11111.1740.70920.0302-0.4455-0.58740.76820.0081-0.94170.17250.10630.040.4037-0.0149-0.0920.48840.08310.712241.5274-40.3581-73.9771
482.2617-0.6431-0.14848.5144-0.09190.7670.14370.283-0.1489-0.6089-0.40230.0188-0.09040.29120.1540.4477-0.03390.04810.4334-0.03480.350829.0177-25.5772-80.3746
491.1264-2.84580.2338.56122.35856.60420.53210.2251-0.2342-0.2414-0.5536-0.29320.9706-0.2405-0.18650.6967-0.05820.17320.78450.01490.547639.6491-64.1556-88.3433
503.92121.068-0.45914.70640.57373.1972-0.1920.78840.1321-0.77920.1501-0.38440.24-0.0652-0.06910.7169-0.04360.21990.68660.01480.642635.2445-62.0808-91.8593
518.70231.62560.2754.14073.51437.8213-0.05620.8003-0.5481-0.9178-0.4032-0.52820.54880.3073-0.01441.1562-0.03590.38881.0652-0.02070.972242.2009-64.2457-97.6702
527.491.8678-0.92772.15691.08832.43150.16160.20040.1187-0.04680.04750.2985-0.7241-0.7046-0.00580.62450.17920.08730.51210.06080.5246-0.74837.1859-27.3443
535.91520.17941.63922.12321.63535.78810.1541.1851-0.50620.16350.05290.2702-0.4437-0.1919-0.48550.42570.12380.05440.5633-0.05090.4588.91312.8605-34.2312
548.6895-0.5012-2.76734.29233.91013.7314-0.2291-0.4299-0.99420.77190.32090.25450.4272-0.5717-0.10540.6110.08060.00160.5610.14650.43677.703-4.7911-24.0558
557.92324.9907-5.15945.7786-4.29949.2509-0.59250.5532-0.2509-0.41510.67-0.01970.0986-0.1738-0.14960.42050.0910.06260.598-0.00040.51680.33140.2113-33.4434
567.35533.4881-2.78973.1816-0.32932.4343-0.266-1.5567-0.76940.0244-0.3307-0.17670.81530.84030.0820.55970.17020.01150.64170.2060.51722.61530.6696-21.3998
572.1747-0.8699-0.34950.75811.69362.15990.1910.71520.11830.0954-0.0438-0.1258-0.074-1.1819-0.20150.56830.08860.04480.71070.09930.51213.59968.9207-22.835
587.25992.69750.8898.92090.05162.36040.29490.07960.4691.1453-0.1918-0.8073-1.28720.2462-0.04361.78140.1885-0.23950.8739-0.04410.60833.05929.74768.2516
593.20232.59881.82032.48231.26221.2589-0.09520.3750.4324-0.2946-0.27120.1167-1.02920.1644-0.07671.28990.0206-0.10290.85340.12040.5666-3.884324.1718-4.2962
601.6050.48980.06595.41523.58432.78260.26790.45340.9786-0.78080.22890.6933-1.6523-1.5951-0.05621.5930.16940.16211.07120.36640.86770.048932.475-6.2993
613.24842.63090.81697.20974.22383.8274-0.031-0.17160.37690.37220.01490.0242-1.1843-0.1249-0.01811.32240.1406-0.0020.84120.22690.499-1.037726.521-1.0877
625.93744.68883.62815.07225.97318.8051-0.271-0.43931.14770.4055-1.03731.7877-1.1072-0.72690.53151.27530.1585-0.10121.14990.10370.905-10.946429.41741.2262
634.2888-0.6656-0.49034.4215-2.88193.4790.18250.3681.0577-0.1950.2122-0.2938-2.08320.5641-0.34421.0769-0.2182-0.071.0280.23280.97720.357740.5375-90.8471
643.7162-0.3197-1.68675.61131.23347.54810.38660.4410.49470.71340.3331-0.2601-1.21590.3716-0.29650.5808-0.06670.01670.5230.1151.08621.14735.7473-78.5255
655.3566-0.38760.50726.8419-0.53687.8295-0.41941.35060.8074-0.91680.06930.1046-0.6083-0.59770.01590.6477-0.1401-0.23220.94360.22770.658115.752529.3966-92.3629
666.08890.10511.56485.8023-0.76849.63780.32611.2279-0.3508-0.54020.3806-0.88960.64621.1077-0.42550.5257-0.0178-0.0130.63090.04030.672423.506923.9331-85.0752
674.45130.041-0.05346.8318-0.82796.9488-0.35820.60430.7162-0.46430.15610.1561-0.79271.1150.14110.5989-0.2201-0.0430.92080.20511.005828.150232.566-85.9238
681.26421.2309-2.00741.9843-3.32816.5582-0.21320.8525-0.0275-1.08190.95850.96070.44820.207-0.15650.7564-0.3057-0.01311.08170.18060.924419.723131.8779-94.9613
692.251-2.2683.02952.9744-2.83867.66570.0064-0.0660.20880.2910.85620.2993-0.5676-0.3664-0.43780.4515-0.0652-0.06390.52730.11940.795213.323825.5727-76.0897
702.8022-1.9160.97395.3574-2.23894.3274-0.27621.58571.4302-1.4379-0.24010.2439-1.62370.68550.46021.6262-0.5443-0.17991.49690.49291.253420.627943.1063-104.5677
712.19-2.77292.75222.5904-2.23225.2738-0.84940.76050.36950.8325-0.22810.7178-0.63990.3645-0.85961.5704-0.1912-0.44951.93120.83462.19780.970850.1329-111.8542
722.08730.6882.3913.4902-1.65194.39170.35580.44680.7318-1.1733-0.05250.8854-0.8120.2849-0.12341.3733-0.1071-0.24011.660.71451.82875.080743.2599-108.3233
731.67131.8591-1.92312.7754-1.64032.78870.73840.04550.7375-0.2898-0.6361-0.6088-1.65520.29130.08551.9496-0.2481-0.32612.06841.12832.8218-4.332454.2284-109.1297
743.16920.73281.2862.7845-2.80914.3902-0.28990.70590.3851-0.83470.5173-0.4105-0.1572-0.78360.04021.9831-0.2033-0.50751.76910.60442.45815.033357.3367-111.1966
752.40521.45490.76982.3314-0.60434.55310.14510.72370.3011-0.48990.38290.53630.4233-1.0425-0.20850.719-0.2173-0.22630.9820.32640.84351.944620.2208-87.675
760.2402-0.44741.0341.9579-0.31472.4449-0.46570.90640.7125-1.45260.96560.72080.4641-0.36893.5321.6982-0.4636-0.4982.20.77661.3355-2.106138.3685-119.6584
774.55920.1856-1.04266.81842.61183.57660.298-1.0697-0.07170.6496-0.42040.56140.3255-0.15060.02220.7228-0.01610.0870.60930.01960.570421.8901-41.2037-65.1075
788.25381.32215.24155.77554.25785.3821-0.4684-0.7098-0.33620.4884-0.6841.55630.5367-1.39220.23470.6537-0.06950.31710.5307-0.03020.838111.6156-44.1742-71.9325
794.32460.55790.12482.5997-0.98821.5732-0.0136-0.1879-0.16970.66860.22890.462-0.2480.5229-0.12120.45690.00640.02570.4535-0.04460.411821.3882-31.3139-70.7881
803.77910.8158-0.03035.1649-3.38152.3293-0.19530.3963-0.8295-0.4633-0.03270.95920.459-0.51160.00320.5918-0.0621-0.04270.5028-0.13990.564819.6019-36.7993-82.1147
810.82191.30662.07378.6516.55037.5744-0.0017-0.34810.14930.7586-0.35480.63680.4456-0.6371-0.05750.42190.02160.160.5616-0.11410.966813.0689-34.7587-71.3349
821.69930.6179-1.16674.3255-1.88421.0974-0.322-0.1934-0.4613-0.7096-0.34260.66870.2829-0.37430.17190.4959-0.03350.07880.5399-0.13250.582718.6299-43.6112-77.8843
83-0.08212.62761.11525.7923-0.10580.1986-0.1210.0299-0.005-0.34470.0636-0.40650.1920.02180.07450.5487-0.01950.15080.5907-0.14520.529829.0847-57.7337-80.8709
847.9342-5.658-0.17136.82740.73421.1455-0.08650.5173-0.66880.4794-0.02760.2322-0.19490.2248-0.08430.6006-0.0660.00310.5484-0.11470.348626.9852-71.8798-81.1762
852.2023-0.30690.8967.37350.10062.30810.03060.2259-0.51871.1405-0.0746-0.84960.5068-0.4935-0.25680.7033-0.18170.10020.6926-0.09830.599733.7509-76.8952-77.0076
864.6467-3.06661.16276.94081.132.6386-0.25720.017-0.0607-0.0230.3317-0.36660.2549-0.0861-0.04430.5169-0.14160.1140.54830.01690.571530.2417-67.535-83.3763
877.7445-5.0654-0.47846.62961.61831.744-0.08470.0931-1.2409-0.54370.177-0.3099-0.04280.5395-0.07430.88480.01270.06990.4984-0.15140.766533.3055-83.569-83.4855
884.3331-4.5546-2.15915.34980.64383.2598-0.00911.1446-0.96550.2096-0.23060.55230.46670.1891-0.10210.8002-0.00040.10980.7204-0.22290.675724.5296-80.7245-86.2288
精密化 TLSグループ
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 8 through 26 )A8 - 26
2X-RAY DIFFRACTION2chain 'A' and (resid 27 through 79 )A27 - 79
3X-RAY DIFFRACTION3chain 'A' and (resid 80 through 175 )A80 - 175
4X-RAY DIFFRACTION4chain 'A' and (resid 176 through 228 )A176 - 228
5X-RAY DIFFRACTION5chain 'A' and (resid 229 through 265 )A229 - 265
6X-RAY DIFFRACTION6chain 'A' and (resid 266 through 308 )A266 - 308
7X-RAY DIFFRACTION7chain 'A' and (resid 309 through 326 )A309 - 326
8X-RAY DIFFRACTION8chain 'B' and (resid 4 through 21 )B4 - 21
9X-RAY DIFFRACTION9chain 'B' and (resid 22 through 55 )B22 - 55
10X-RAY DIFFRACTION10chain 'B' and (resid 56 through 65 )B56 - 65
11X-RAY DIFFRACTION11chain 'B' and (resid 66 through 75 )B66 - 75
12X-RAY DIFFRACTION12chain 'B' and (resid 76 through 126 )B76 - 126
13X-RAY DIFFRACTION13chain 'B' and (resid 127 through 170 )B127 - 170
14X-RAY DIFFRACTION14chain 'B' and (resid 171 through 176 )B171 - 176
15X-RAY DIFFRACTION15chain 'C' and (resid 8 through 79 )C8 - 79
16X-RAY DIFFRACTION16chain 'C' and (resid 80 through 104 )C80 - 104
17X-RAY DIFFRACTION17chain 'C' and (resid 105 through 195 )C105 - 195
18X-RAY DIFFRACTION18chain 'C' and (resid 196 through 228 )C196 - 228
19X-RAY DIFFRACTION19chain 'C' and (resid 229 through 265 )C229 - 265
20X-RAY DIFFRACTION20chain 'C' and (resid 266 through 308 )C266 - 308
21X-RAY DIFFRACTION21chain 'C' and (resid 309 through 326 )C309 - 326
22X-RAY DIFFRACTION22chain 'D' and (resid 4 through 55 )D4 - 55
23X-RAY DIFFRACTION23chain 'D' and (resid 56 through 65 )D56 - 65
24X-RAY DIFFRACTION24chain 'D' and (resid 66 through 75 )D66 - 75
25X-RAY DIFFRACTION25chain 'D' and (resid 76 through 126 )D76 - 126
26X-RAY DIFFRACTION26chain 'D' and (resid 127 through 174 )D127 - 174
27X-RAY DIFFRACTION27chain 'E' and (resid 8 through 26 )E8 - 26
28X-RAY DIFFRACTION28chain 'E' and (resid 27 through 104 )E27 - 104
29X-RAY DIFFRACTION29chain 'E' and (resid 105 through 195 )E105 - 195
30X-RAY DIFFRACTION30chain 'E' and (resid 196 through 228 )E196 - 228
31X-RAY DIFFRACTION31chain 'E' and (resid 229 through 265 )E229 - 265
32X-RAY DIFFRACTION32chain 'E' and (resid 266 through 308 )E266 - 308
33X-RAY DIFFRACTION33chain 'E' and (resid 309 through 326 )E309 - 326
34X-RAY DIFFRACTION34chain 'F' and (resid 4 through 55 )F4 - 55
35X-RAY DIFFRACTION35chain 'F' and (resid 56 through 75 )F56 - 75
36X-RAY DIFFRACTION36chain 'F' and (resid 76 through 126 )F76 - 126
37X-RAY DIFFRACTION37chain 'F' and (resid 127 through 173 )F127 - 173
38X-RAY DIFFRACTION38chain 'G' and (resid 1 through 32 )G1 - 32
39X-RAY DIFFRACTION39chain 'G' and (resid 33 through 98 )G33 - 98
40X-RAY DIFFRACTION40chain 'G' and (resid 99 through 149 )G99 - 149
41X-RAY DIFFRACTION41chain 'G' and (resid 150 through 231 )G150 - 231
42X-RAY DIFFRACTION42chain 'H' and (resid 1 through 17 )H1 - 17
43X-RAY DIFFRACTION43chain 'H' and (resid 18 through 32 )H18 - 32
44X-RAY DIFFRACTION44chain 'H' and (resid 33 through 44 )H33 - 44
45X-RAY DIFFRACTION45chain 'H' and (resid 45 through 64 )H45 - 64
46X-RAY DIFFRACTION46chain 'H' and (resid 65 through 83 )H65 - 83
47X-RAY DIFFRACTION47chain 'H' and (resid 84 through 99 )H84 - 99
48X-RAY DIFFRACTION48chain 'H' and (resid 100 through 121 )H100 - 121
49X-RAY DIFFRACTION49chain 'H' and (resid 122 through 149 )H122 - 149
50X-RAY DIFFRACTION50chain 'H' and (resid 150 through 215 )H150 - 215
51X-RAY DIFFRACTION51chain 'H' and (resid 216 through 231 )H216 - 231
52X-RAY DIFFRACTION52chain 'I' and (resid 1 through 25 )I1 - 25
53X-RAY DIFFRACTION53chain 'I' and (resid 26 through 38 )I26 - 38
54X-RAY DIFFRACTION54chain 'I' and (resid 39 through 61 )I39 - 61
55X-RAY DIFFRACTION55chain 'I' and (resid 62 through 75 )I62 - 75
56X-RAY DIFFRACTION56chain 'I' and (resid 76 through 90 )I76 - 90
57X-RAY DIFFRACTION57chain 'I' and (resid 91 through 113 )I91 - 113
58X-RAY DIFFRACTION58chain 'I' and (resid 114 through 128 )I114 - 128
59X-RAY DIFFRACTION59chain 'I' and (resid 129 through 150 )I129 - 150
60X-RAY DIFFRACTION60chain 'I' and (resid 151 through 163 )I151 - 163
61X-RAY DIFFRACTION61chain 'I' and (resid 164 through 197 )I164 - 197
62X-RAY DIFFRACTION62chain 'I' and (resid 198 through 214 )I198 - 214
63X-RAY DIFFRACTION63chain 'J' and (resid 1 through 16 )J1 - 16
64X-RAY DIFFRACTION64chain 'J' and (resid 17 through 32 )J17 - 32
65X-RAY DIFFRACTION65chain 'J' and (resid 33 through 44 )J33 - 44
66X-RAY DIFFRACTION66chain 'J' and (resid 45 through 64 )J45 - 64
67X-RAY DIFFRACTION67chain 'J' and (resid 65 through 84 )J65 - 84
68X-RAY DIFFRACTION68chain 'J' and (resid 85 through 98 )J85 - 98
69X-RAY DIFFRACTION69chain 'J' and (resid 99 through 121 )J99 - 121
70X-RAY DIFFRACTION70chain 'J' and (resid 122 through 134 )J122 - 134
71X-RAY DIFFRACTION71chain 'J' and (resid 135 through 172 )J135 - 172
72X-RAY DIFFRACTION72chain 'J' and (resid 173 through 192 )J173 - 192
73X-RAY DIFFRACTION73chain 'J' and (resid 193 through 215 )J193 - 215
74X-RAY DIFFRACTION74chain 'J' and (resid 216 through 231 )J216 - 231
75X-RAY DIFFRACTION75chain 'K' and (resid 1 through 102 )K1 - 102
76X-RAY DIFFRACTION76chain 'K' and (resid 103 through 214 )K103 - 214
77X-RAY DIFFRACTION77chain 'L' and (resid 1 through 12 )L1 - 12
78X-RAY DIFFRACTION78chain 'L' and (resid 13 through 25 )L13 - 25
79X-RAY DIFFRACTION79chain 'L' and (resid 26 through 38 )L26 - 38
80X-RAY DIFFRACTION80chain 'L' and (resid 39 through 61 )L39 - 61
81X-RAY DIFFRACTION81chain 'L' and (resid 62 through 75 )L62 - 75
82X-RAY DIFFRACTION82chain 'L' and (resid 76 through 90 )L76 - 90
83X-RAY DIFFRACTION83chain 'L' and (resid 91 through 128 )L91 - 128
84X-RAY DIFFRACTION84chain 'L' and (resid 129 through 150 )L129 - 150
85X-RAY DIFFRACTION85chain 'L' and (resid 151 through 163 )L151 - 163
86X-RAY DIFFRACTION86chain 'L' and (resid 164 through 186 )L164 - 186
87X-RAY DIFFRACTION87chain 'L' and (resid 187 through 197 )L187 - 197
88X-RAY DIFFRACTION88chain 'L' and (resid 198 through 214 )L198 - 214

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万見について

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お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月:コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか? / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例:em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る