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- PDB-5k9q: Crystal structure of multidonor HV1-18-class broadly neutralizing... -

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Open data

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Basic information

Entry

Database: PDB / ID: 5k9q

Title

Crystal structure of multidonor HV1-18-class broadly neutralizing Influenza A antibody 16.a.26 in complex with A/Hong Kong/1-4-MA21-1/1968 (H3N2) Hemagglutinin

Components

(Hemagglutinin ...) x 2
16.a.26 Heavy chain
16.a.26 Light chain

Keywords

IMMUNE SYSTEM / HV1-18 (QxxV) / multidonor / H5 /

universal influenza vaccine

Function / homology

Function and homology information

viral budding from plasma membrane / clathrin-dependent endocytosis of virus by host cell / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane /

viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane /

membrane
Similarity search - Function

Biological species

Influenza A virus

Homo sapiens (human)

Method

X-RAY DIFFRACTION /

SYNCHROTRON / Resolution: 2.503 Å

Authors

Joyce, M.G. / Thomas, P.V. / Wheatley, A.K. / McDermott, A.B. / Mascola, J.R. / Kwong, P.D.

Citation

Journal: Cell(Cambridge,Mass.) / Year: 2016
Title: Vaccine-Induced Antibodies that Neutralize Group 1 and Group 2 Influenza A Viruses.
Authors: Joyce, M.G. / Wheatley, A.K. / Thomas, P.V. / Chuang, G.Y. / Soto, C. / Bailer, R.T. / Druz, A. / Georgiev, I.S. / Gillespie, R.A. / Kanekiyo, M. / Kong, W.P. / Leung, K. / Narpala, S.N. / ...

History

Deposition	Jun 1, 2016	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Nov 30, 2016	Provider: repository / Type: Initial release
Revision 1.1	Jul 29, 2020	Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Structure summary Category: chem_comp / citation ...chem_comp / citation / entity / pdbx_chem_comp_identifier / pdbx_entity_nonpoly / struct_conn / struct_site / struct_site_gen Item: _chem_comp.name / _chem_comp.type ..._chem_comp.name / _chem_comp.type / _citation.journal_id_CSD / _entity.pdbx_description / _pdbx_entity_nonpoly.name / _struct_conn.pdbx_role Description: Carbohydrate remediation / Provider: repository / Type: Remediation

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Structure visualization

Structure viewer	Molecule: MolmilJmol/JSmol

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Downloads & links

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Download

PDBx/mmCIF format	5k9q.cif.gz	2.1 MB	Display	PDBx/mmCIF format
PDB format	pdb5k9q.ent.gz	1.8 MB	Display	PDB format
PDBx/mmJSON format	5k9q.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/k9/5k9q ftp://data.pdbj.org/pub/pdb/validation_reports/k9/5k9q	HTTPS FTP

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Related structure data

Related structure data	5k9jC 5k9kC 5k9oC 5kanC 5kaqC C: citing same article (ref.)
Similar structure data	4ubd-1 5kan-d 4ubd-2 5kaq-d 2753 4py8-1 4kvn-1 4fqv-d Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#170 - Feb 2014 Broadly Neutralizing Antibodies similarity (6) #21 - Sep 2001 Antibodies similarity (1) #256 - Apr 2021 SARS-CoV-2 Spike and Antibodies similarity (1) #76 - Apr 2006 Hemagglutinin similarity (5) #259 - Jul 2021 Designed Proteins and Citizen Science similarity (5) #62 - Feb 2005 Major Histocompatibility Complex similarity (5) #63 - Mar 2005 T-Cell Receptor similarity (5) #200 - Aug 2016 Quasisymmetry in Icosahedral Viruses similarity (1)

PDB pages

PDBj /

wwPDB /

NCBI

Related items in Molecule of the Month

#170 - Feb 2014
Broadly Neutralizing Antibodies
similarity (6)
#21 - Sep 2001
Antibodies
similarity (1)
#256 - Apr 2021
SARS-CoV-2 Spike and Antibodies
similarity (1)
#76 - Apr 2006
Hemagglutinin
similarity (5)
#259 - Jul 2021
Designed Proteins and Citizen Science
similarity (5)
#62 - Feb 2005
Major Histocompatibility Complex
similarity (5)
#63 - Mar 2005
T-Cell Receptor
similarity (5)
#200 - Aug 2016
Quasisymmetry in Icosahedral Viruses
similarity (1)

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Assembly

Deposited unit

A: Hemagglutinin HA1

B: Hemagglutinin HA2

C: Hemagglutinin HA1

D: Hemagglutinin HA2

E: Hemagglutinin HA1

F: Hemagglutinin HA2

G: 16.a.26 Heavy chain

H: 16.a.26 Heavy chain

I: 16.a.26 Light chain

J: 16.a.26 Heavy chain

K: 16.a.26 Light chain

L: 16.a.26 Light chain

M: Hemagglutinin HA1

N: Hemagglutinin HA2

O: Hemagglutinin HA1

P: Hemagglutinin HA2

Q: Hemagglutinin HA1

R: Hemagglutinin HA2

S: 16.a.26 Heavy chain

T: 16.a.26 Light chain

U: 16.a.26 Heavy chain

V: 16.a.26 Light chain

X: 16.a.26 Heavy chain

Y: 16.a.26 Light chain

hetero molecules

627 kDa, 24 polymers
Search similar-shape structures of this assembly by Omokage search (details)

A: Hemagglutinin HA1

B: Hemagglutinin HA2

C: Hemagglutinin HA1

D: Hemagglutinin HA2

E: Hemagglutinin HA1

F: Hemagglutinin HA2

G: 16.a.26 Heavy chain

H: 16.a.26 Heavy chain

I: 16.a.26 Light chain

J: 16.a.26 Heavy chain

K: 16.a.26 Light chain

L: 16.a.26 Light chain

hetero molecules

defined by author&software
313 kDa, 12 polymers
Search similar-shape structures of this assembly by Omokage search (details)

M: Hemagglutinin HA1

N: Hemagglutinin HA2

O: Hemagglutinin HA1

P: Hemagglutinin HA2

Q: Hemagglutinin HA1

R: Hemagglutinin HA2

S: 16.a.26 Heavy chain

T: 16.a.26 Light chain

U: 16.a.26 Heavy chain

V: 16.a.26 Light chain

X: 16.a.26 Heavy chain

Y: 16.a.26 Light chain

hetero molecules

defined by author&software
313 kDa, 12 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	121.808, 233.909, 302.452
Angle α, β, γ (deg.)	90.00, 90.00, 90.00
Int Tables number	19
Space group name H-M	P2₁2₁2₁

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Components

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Hemagglutinin ... , 2 types, 12 molecules ACEMOQBDFNPR

#1: Protein	Hemagglutinin HA1 Mass: 35225.617 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Influenza A virus / Strain: A/Hong Kong/1/1968 H3N2 / Gene: HA / Production host: Homo sapiens (human) / References: UniProt: Q91MA7
#2: Protein	Hemagglutinin HA2 Mass: 19728.725 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Influenza A virus (strain A/Hong Kong/1/1968 H3N2) Strain: A/Hong Kong/1/1968 H3N2 / Gene: HA / Production host: Homo sapiens (human) / References: UniProt: Q91MA7

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Antibody , 2 types, 12 molecules GHJSUXIKLTVY

#3: Antibody	16.a.26 Heavy chain Mass: 24567.611 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#4: Antibody	16.a.26 Light chain Mass: 23585.182 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)

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Sugars / Non-polymers , 2 types, 1705 molecules

#5: Sugar

...
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose /

N-Acetylglucosamine

Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 36
Source method: isolated from a genetically manipulated source
Formula: C₈H₁₅NO₆

Identifier	Type	Program
DGlcpNAcb	CONDENSED IUPAC CARBOHYDRATE SYMBOL	GMML 1.0
N-acetyl-b-D-glucopyranosamine	COMMON NAME	GMML 1.0
b-D-GlcpNAc	IUPAC CARBOHYDRATE SYMBOL	PDB-CARE 1.0
GlcNAc	SNFG CARBOHYDRATE SYMBOL	GMML 1.0

#6: Water

ChemComp-HOH / water /

Water

Mass: 18.015 Da / Num. of mol.: 1669 / Source method: isolated from a natural source / Formula: H₂O

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Experimental details

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 3.5 Å³/Da / Density % sol: 64.81 %
Crystal grow	Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 0.01M L-cysteine, 0.09M sodium cacodylate, pH 6.5, 0.18M magnesium acetate, and 7.9% (w/v) PEG-8000

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Data collection

Diffraction	Mean temperature: 100 K
Diffraction source	Source: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
Detector	Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Feb 3, 2015
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1 Å / Relative weight: 1
Reflection	Resolution: 2.5→50 Å / Num. obs: 244485 / % possible obs: 82.55 % / Redundancy: 4.1 % / Net I/σ(I): 14.05

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Processing

Software

Name	Version	Classification
PHENIX	(1.10.1_2155: ???)	refinement
PDB_EXTRACT	3.2	data extraction
HKL-2000		data reduction
HKL-2000		data scaling
PHASER		phasing

Refinement

Resolution: 2.503→49.277 Å / SU ML: 0.3 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 23.84 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.218	12282	5.02 %
R_work	0.182	-	-
obs	0.1838	244444	82.54 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 2.503→49.277 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	43247	0	504	1669	45420

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.003	44707
X-RAY DIFFRACTION	f_angle_d	0.635	60799
X-RAY DIFFRACTION	f_dihedral_angle_d	14.308	26747
X-RAY DIFFRACTION	f_chiral_restr	0.044	6826
X-RAY DIFFRACTION	f_plane_restr	0.004	7855

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.503-2.5315	0.3013	192	0.2826	3454	X-RAY DIFFRACTION	37
2.5315-2.5613	0.3125	300	0.2765	4536	X-RAY DIFFRACTION	49
2.5613-2.5925	0.362	303	0.2807	5075	X-RAY DIFFRACTION	55
2.5925-2.6253	0.327	269	0.2795	5597	X-RAY DIFFRACTION	60
2.6253-2.6599	0.3304	316	0.2687	6010	X-RAY DIFFRACTION	65
2.6599-2.6963	0.3146	332	0.2755	6398	X-RAY DIFFRACTION	69
2.6963-2.7348	0.3058	356	0.2701	6684	X-RAY DIFFRACTION	72
2.7348-2.7756	0.3246	425	0.2695	7090	X-RAY DIFFRACTION	76
2.7756-2.819	0.305	394	0.263	7438	X-RAY DIFFRACTION	80
2.819-2.8652	0.3304	406	0.2553	7707	X-RAY DIFFRACTION	83
2.8652-2.9146	0.287	392	0.2471	7845	X-RAY DIFFRACTION	84
2.9146-2.9676	0.2867	428	0.251	8097	X-RAY DIFFRACTION	86
2.9676-3.0247	0.2854	448	0.2449	8200	X-RAY DIFFRACTION	89
3.0247-3.0864	0.2645	467	0.2328	8358	X-RAY DIFFRACTION	90
3.0864-3.1535	0.2784	431	0.2279	8509	X-RAY DIFFRACTION	91
3.1535-3.2268	0.2691	476	0.2223	8589	X-RAY DIFFRACTION	92
3.2268-3.3075	0.2704	444	0.2093	8674	X-RAY DIFFRACTION	93
3.3075-3.3969	0.2404	471	0.2005	8695	X-RAY DIFFRACTION	93
3.3969-3.4969	0.2447	485	0.1911	8628	X-RAY DIFFRACTION	93
3.4969-3.6097	0.2251	466	0.1891	8687	X-RAY DIFFRACTION	93
3.6097-3.7387	0.2218	464	0.178	8687	X-RAY DIFFRACTION	93
3.7387-3.8883	0.2112	417	0.1767	8814	X-RAY DIFFRACTION	93
3.8883-4.0652	0.1872	451	0.1583	8842	X-RAY DIFFRACTION	94
4.0652-4.2794	0.1645	439	0.1457	8828	X-RAY DIFFRACTION	94
4.2794-4.5473	0.1534	436	0.1341	8844	X-RAY DIFFRACTION	94
4.5473-4.8982	0.16	471	0.1347	8839	X-RAY DIFFRACTION	93
4.8982-5.3905	0.1723	459	0.1386	8810	X-RAY DIFFRACTION	93
5.3905-6.1693	0.183	469	0.1537	8794	X-RAY DIFFRACTION	92
6.1693-7.7677	0.2045	423	0.1617	8794	X-RAY DIFFRACTION	91
7.7677-49.2869	0.1604	452	0.1391	8639	X-RAY DIFFRACTION	87

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	7.4495	-6.6342	4.8435	6.8552	-6.8324	6.5103	-0.3003	-0.6072	0.0481	0.4065	0.8691	0.7068	-0.198	-0.4561	-0.3161	0.3922	0.0663	0.1024	0.4743	-0.1014	0.4044	-42.4795	9.1497	61.0828
2	0.9616	-1.5955	-0.3166	4.7991	0.8589	0.4249	-0.125	-0.0812	-0.1394	0.3061	0.0328	0.4516	0.081	-0.0583	0.1257	0.309	-0.006	0.0449	0.4723	-0.0222	0.3337	-26.0516	-33.1128	55.3088
3	1.2363	-1.4181	0.0229	1.4916	0.3753	1.57	0.0002	-0.0082	-0.1966	0.061	0.0139	0.1636	0.2495	0.0314	0.0034	0.3594	-0.0137	-0.0119	0.3808	-0.0113	0.3425	-9.2485	-57.8908	46.6536
4	1.6348	-1.3056	0.485	4.1406	-1.7886	3.9338	-0.0238	-0.2466	-0.1497	0.3294	0.1727	0.1768	0.3821	0.257	-0.1575	0.4583	0.0614	-0.0533	0.4213	-0.0521	0.2875	-0.0011	-63.7538	55.2176
5	1.4856	-0.9664	0.1882	2.5664	-0.2348	1.6806	-0.0056	0.0544	0.0026	0.0707	0.1169	-0.1332	0.0227	0.1115	-0.1272	0.3317	-0.0013	-0.0006	0.3711	-0.0452	0.3185	-2.2919	-53.1713	45.4324
6	1.5555	-0.9998	-0.5825	4.0242	0.5137	1.1384	-0.1049	-0.0637	-0.1238	0.0013	-0.0639	0.3342	-0.0458	-0.1202	0.0947	0.2837	0.0018	0.0053	0.4284	-0.0329	0.2936	-26.3767	-32.0446	54.7588
7	2.225	-2.3815	1.0584	5.9227	-0.9328	0.3044	-0.2255	-0.604	0.0539	0.2089	0.53	0.144	-0.3064	-0.2136	-0.3413	0.3301	-0.0096	0.0396	0.5064	-0.0515	0.2843	-28.3484	-14.1422	61.2949
8	6.159	-3.1253	-3.6845	5.362	5.5623	5.8167	-0.2399	0.091	-0.4717	0.0232	-0.444	0.1796	0.4809	0.1508	0.5984	0.9524	0.4085	0.021	1.8836	0.0315	0.6513	-48.1289	10.3143	70.8643
9	4.9241	-1.496	1.1955	4.7935	-3.4203	4.7182	-0.1773	-0.9868	0.1585	0.3075	-0.097	1.3179	-0.3857	-0.673	0.4037	0.3759	0.0668	0.0614	0.6238	-0.0673	0.6816	-43.2461	8.9444	60.5979
10	0.4508	-0.8508	0.0623	4.2032	-0.0112	0.9275	0.0486	-0.0916	0.0875	-0.1445	0.0133	0.1596	-0.1306	-0.1289	0.0018	0.3444	-0.0291	-0.0326	0.4981	-0.0824	0.3494	-32.5099	-1.4517	51.3355
11	3.4676	0.0242	0.7516	4.0969	-5.3383	9.0974	-0.193	-0.2178	-0.42	-0.1899	0.2699	-0.3409	0.8582	-0.1647	0.0723	0.4878	0.0144	-0.0778	0.5966	-0.0387	0.4569	-12.3936	-40.5183	65.5396
12	2.8372	-4.4244	1.2501	7.7236	-2.2478	1.0951	-0.0931	0.0055	0.2205	0.3101	-0.0648	-0.1743	-0.1899	0.0438	0.124	0.3374	-0.0395	-0.0219	0.4411	-0.0661	0.3176	-22.3135	-3.6832	60.8234
13	2.1116	0.427	-0.2318	4.2537	-0.2923	2.3776	0.0624	0.3104	0.3068	0.0517	-0.2716	0.5351	-0.741	-0.3534	0.176	0.667	0.1441	-0.0699	0.5311	-0.0519	0.6375	-42.0445	27.878	52.163
14	1.2057	-1.4213	0.003	2.1852	0.0592	0.3708	0.1849	0.1332	0.1544	-0.2447	-0.1739	-0.2087	-0.02	0.1171	0.0106	0.3847	-0.015	0.0158	0.507	-0.0377	0.364	8.458	-15.9975	47.551
15	1.3326	0.0019	0.5867	2.1524	-0.0898	1.1873	0.1165	-0.2134	-0.15	0.3273	-0.024	-0.2743	0.2412	0.0734	-0.0948	0.36	0.0055	-0.0239	0.5605	-0.018	0.3915	23.3413	-44.3836	59.225
16	1.9495	-3.6816	-0.2031	8.0843	-0.6842	0.5105	0.0293	0.0531	0.159	-0.4583	0.0289	0.075	-0.0231	0.0097	-0.0701	0.3099	-0.0162	0.0059	0.4442	-0.0565	0.3375	-3.0724	-6.6566	48.2104
17	7.8931	-2.0288	3.1058	5.4759	-0.8336	8.6761	0.2304	1.289	0.6058	-0.7481	-0.2062	0.2038	-0.7233	-0.0185	0.0153	0.7017	0.0143	-0.0241	0.5234	0.0601	0.5498	-14.7341	21.1055	42.5899
18	3.1193	-3.4225	1.8079	3.5122	-2.4328	3.754	-0.0525	0.2595	0.3188	0.2862	-0.0694	-0.3057	-0.6408	0.2139	0.0313	0.4563	-0.0329	0.017	0.4851	-0.021	0.4493	-7.0743	19.1458	51.0179
19	0.9956	1.3986	0.7464	7.0166	0.7979	2.78	0.0867	0.0025	-0.0688	0.558	0.1222	-0.1148	0.2939	0.0837	-0.1555	0.3209	0.008	0.0371	0.6454	-0.0526	0.4669	4.7574	-24.9211	55.8292
20	0.6073	-1.6741	0.0434	8.8291	-0.4305	0.4342	0.0148	0.0276	0.0345	0.0616	0.0015	0.2512	-0.0804	-0.0615	-0.0079	0.3045	0.0045	-0.0211	0.4224	-0.0253	0.3224	-12.6894	0.3709	56.1025
21	4.2398	-0.1791	-0.2806	4.8082	0.806	4.3629	0.1433	-0.1924	0.6741	-0.071	-0.2205	-0.0925	-0.6538	-0.263	0.1063	0.7281	0.082	-0.0144	0.3971	-0.0139	0.6176	-19.3198	37.8735	53.2852
22	7.2601	0.2478	0.0143	1.5935	0.484	1.6727	-0.1245	-0.0245	0.0066	0.3871	0.2312	0.1645	-0.5161	0.3407	-0.119	0.5838	-0.047	0.0506	0.5033	-0.1383	0.2789	-15.143	21.8719	77.7922
23	0.5143	-0.63	0.0335	3.9241	-0.4109	1.0524	-0.0657	-0.1682	-0.0477	0.4539	-0.0666	-0.336	0.357	0.262	0.1162	0.5118	0.0861	-0.0233	0.6485	0.0017	0.3383	-0.5526	-36.5317	86.3733
24	4.2666	-0.8406	1.4205	2.1574	-0.7381	2.7005	0.0065	-0.1799	-0.4963	0.32	0.1011	0.069	1.0639	-0.038	-0.1279	0.9973	0.1082	0.0166	0.5246	0.075	0.4574	-2.5994	-62.3516	83.9151
25	4.662	-0.9272	1.1327	2.3621	-0.6546	3.2679	0.0728	0.2512	-0.1364	-0.1429	-0.1104	-0.3628	0.724	0.5302	0.0178	0.8253	0.2185	0.0041	0.614	0.0462	0.4225	5.2522	-58.9303	77.397
26	0.0923	-0.5944	-0.0002	2.8726	-0.7508	1.4008	-0.1135	-0.0816	-0.0036	0.3698	0.1399	0.082	0.1186	0.0255	-0.0104	0.4704	0.052	-0.0291	0.5654	-0.0336	0.3358	-4.8954	-30.2813	84.5126
27	1.3544	-1.8205	-0.0357	4.1472	-0.1323	0.925	-0.0228	-0.3534	0.2644	-0.1924	0.2984	-0.4417	-0.044	-0.0207	-0.3272	0.3765	0.0015	-0.0486	0.5344	-0.0873	0.3439	-6.7305	-4.0619	76.1721
28	1.2252	-0.5353	0.4062	6.1381	1.5707	1.7375	-0.0112	-0.0731	0.1616	-0.0936	-0.1533	0.0204	-0.3591	-0.021	0.1924	0.5031	0.0025	0.0024	0.5158	-0.1027	0.3583	-20.0354	16.3475	79.5758
29	6.6462	-0.6281	-1.2066	2.2959	1.7079	2.8455	-0.2918	-0.6267	-0.4747	0.3138	0.211	0.2468	0.0299	0.3149	0.065	0.5959	0.0337	-0.0632	0.6371	-0.0105	0.3051	-3.5361	-27.8294	74.5935
30	1.4951	-2.4211	-0.5501	5.8016	1.0404	0.654	0.1657	0.1509	0.0677	-0.0796	-0.2371	0.0887	-0.2363	-0.0823	0.0333	0.4061	0.0069	-0.014	0.5333	-0.085	0.3324	-16.1189	6.3656	68.6512
31	5.176	-0.0739	-0.8818	2.298	0.8194	6.7001	-0.0268	0.2044	0.4309	0.2898	-0.0103	0.4743	-0.9061	-0.7897	0.077	0.6444	0.1292	0.034	0.5741	-0.0934	0.5935	-34.9608	33.5727	74.2269
32	2.6908	2.2724	2.5119	4.5771	0.1347	3.7082	-0.0101	-0.1375	0.9588	0.113	0.2402	-0.7812	-0.7905	0.8489	-0.0068	0.6111	-0.1405	-0.1163	0.6033	-0.1653	1.0726	21.0736	29.1495	65.4778
33	4.7144	-0.5986	0.7027	2.5522	-0.2376	5.756	-0.1006	-0.1184	0.4729	-0.0094	0.2849	-0.4209	-0.3889	0.2258	0.0019	0.4595	-0.0582	-0.0163	0.4657	-0.1476	0.6803	14.5436	20.961	62.47
34	5.1985	-1.452	1.7862	5.1478	-0.1672	2.0666	0.0982	-0.9565	0.3541	0.4933	0.3474	0.508	-0.0952	-0.0517	-0.2266	0.4733	-0.0116	0.1146	0.6284	-0.2138	0.7046	4.6663	20.5856	66.5359
35	3.5855	-3.2841	-2.251	4.3155	-0.6825	8.246	-0.3633	-0.3979	1.376	0.2309	0.3089	-0.0327	-1.2134	0.0918	-0.0764	0.717	-0.0589	-0.1135	0.6164	-0.1743	0.8749	9.5607	28.1927	64.1672
36	5.3715	0.748	2.6648	2.5575	3.3023	9.5359	0.1059	-0.6578	0.4851	0.5324	0.3842	-0.7029	-0.0781	-0.0181	-0.1464	0.6342	0.0227	-0.0929	0.569	-0.2078	0.7771	17.3842	23.3846	71.2047
37	3.4967	-0.5462	0.7863	4.4423	1.4658	3.4248	0.0209	0.5676	0.3428	0.0135	0.1143	-0.0286	-0.0017	0.4304	-0.0959	0.3395	-0.0368	0.0578	0.4842	-0.0098	0.4239	10.789	12.3688	55.397
38	0.2275	-0.7029	-0.6625	2.7837	1.3385	4.9331	-0.3363	-0.7897	0.7509	0.7535	-0.4102	-1.3852	-0.5204	0.127	0.0623	0.9388	-0.1302	-0.316	0.8629	-0.3502	1.4441	28.8233	32.5932	77.5404
39	1.5402	0.0501	-3.5255	3.6096	-0.6279	7.9957	-1.3463	-1.1587	1.5294	0.9725	-0.0299	-1.9286	0.0549	2.4276	-1.0802	1.2784	-0.0634	-0.6291	1.704	-0.478	2.196	56.2425	21.2536	85.9087
40	4.3297	-3.0141	0.8326	5.8566	0.7231	2.4315	-0.1036	0.2399	1.6665	-0.0337	-0.124	-1.4403	-0.8755	0.8346	0.4333	1.0345	-0.1785	-0.1669	1.3052	-0.3901	1.6102	43.6313	25.0262	77.8111
41	2.7382	2.7528	2.1441	5.101	1.6473	4.3948	-0.8596	1.0422	0.9632	1.2204	-0.0731	-1.4358	-1.0999	1.4386	0.7561	1.0006	-0.1911	-0.2061	1.611	-0.2534	2.1397	53.8211	27.8648	77.9218
42	2.6641	-0.3785	-0.4308	1.8499	0.1211	3.9382	0.2018	0.2238	0.1444	-0.326	-0.1518	0.0699	-0.0534	-0.2168	-0.0176	0.3786	0.0505	-0.0107	0.4082	-0.0282	0.3917	-38.935	-2.4359	24.9163
43	1.8466	0.1282	0.1284	6.0254	2.867	6.345	0.0529	0.3649	0.4631	-0.3505	0.233	-0.81	-0.3362	0.4856	-0.1465	0.3178	-0.0007	0.0085	0.4372	0.0152	0.4226	-29.787	1.1144	32.2402
44	9.6932	1.433	-4.1684	6.3428	-0.4037	9.2593	0.2656	-0.0222	0.4783	-0.3582	-0.1928	-0.1178	-0.5085	-0.4016	-0.1476	0.4583	0.0363	-0.0135	0.3836	-0.0176	0.3921	-36.9554	5.6458	28.4189
45	3.2813	0.6274	-1.8584	4.4603	-2.8846	5.7944	0.2165	0.0282	0.141	-0.5292	-0.2976	-0.3746	-0.4241	0.3525	0.3113	0.4309	0.088	0.0378	0.4713	0.0207	0.365	-31.6391	-1.1615	20.8071
46	0.199	0.0228	-0.4883	1.4319	-0.5347	4.4107	0.084	-0.0699	-0.0267	-0.0462	-0.0415	0.1101	-0.0371	0.0201	-0.0185	0.2889	0.0209	0.0198	0.4094	-0.0428	0.3413	-35.874	-9.0187	39.0848
47	2.7522	-0.8414	-1.3046	3.359	-1.1439	6.354	0.4315	1.3375	-0.0179	-0.9723	-0.9333	-0.0729	0.102	0.4841	-0.0118	0.7271	0.1925	0.053	0.6625	0.0866	0.4446	-35.8501	-0.324	6.0264
48	5.2156	2.3377	-1.1728	2.9005	0.6478	1.9023	0.2473	1.3884	-1.0186	-1.0925	-0.3247	-0.6465	0.6801	-0.5929	0.1241	1.6651	0.9005	-0.3167	1.0182	-0.1196	0.5519	-38.8321	-24.6526	-11.6021
49	2.5633	1.3687	0.4612	3.458	1.2511	1.8626	0.4295	0.5465	-0.0904	-0.9154	-0.6228	0.4306	-0.4766	-0.3726	0.1275	1.0003	0.3923	-0.1274	0.8565	-0.1194	0.4595	-42.3801	-17.1423	-3.114
50	8.3708	-0.6239	-5.1646	3.046	2.4391	9.5475	-0.0912	-0.8112	0.083	0.528	0.1603	-0.5505	0.4284	1.0005	-0.1743	0.491	-0.0294	-0.0325	0.6264	-0.0933	0.5828	26.0169	-0.4865	69.7438
51	5.9474	-0.3639	-1.7103	2.1977	0.2788	2.5137	0.0958	-0.4936	-0.162	0.123	0.0038	-0.1335	0.0741	0.3355	-0.0833	0.3752	0.0391	-0.0273	0.5393	-0.1115	0.4523	17.2488	1.3595	65.5344
52	4.2219	-0.3723	1.0874	8.3338	0.3599	5.7717	-0.1425	0.4127	0.1279	-0.5356	0.1346	-0.6977	-0.062	0.6259	-0.0198	0.3958	0.027	0.0389	0.5893	-0.1045	0.4118	21.8169	2.4345	58.7299
53	4.0375	-4.8575	1.0403	6.3722	0.4735	4.5271	0.1885	0.6753	-0.2448	0.0635	-0.0604	-0.7402	0.5495	0.5296	-0.0625	0.4121	-0.0194	-0.0095	0.6554	-0.1829	0.5681	28.9886	3.91	62.7713
54	8.0788	-0.4479	2.8546	2.5337	0.5822	4.1743	-0.2506	-1.1271	0.9028	0.115	0.0662	0.1036	-0.0478	-0.2973	0.328	0.4171	-0.019	0.0657	0.6442	-0.1908	0.5302	13.4039	8.0126	69.1283
55	1.9754	-1.9172	-0.2879	4.475	0.1706	0.0945	-0.0069	-0.7939	-0.5304	0.7119	0.3745	0.0828	0.4706	0.6902	-0.0284	0.4804	0.1709	-0.07	1.0435	-0.1861	0.7525	42.3371	1.4015	73.2667
56	2.2805	1.0632	0.4599	6.9009	5.3199	4.4443	-0.8127	-1.3538	1.3619	1.0482	0.4959	-0.3856	-0.3888	0.1233	0.2551	1.2448	0.3278	-0.3942	1.6328	-0.5816	1.3716	46.4681	16.6935	88.5208
57	1.0276	-0.0467	1.5977	1.2511	0.8314	9.2163	-1.2404	-1.3375	-0.5881	1.8349	0.0833	-0.5478	-0.2239	-0.4333	0.7726	1.5282	0.3387	-0.0601	2.0197	-0.2649	1.1685	39.7175	15.0074	94.5424
58	5.4698	1.9811	1.9366	2.2099	2.1096	2.1256	-0.2064	0.5896	0.84	-0.173	-0.1807	-1.079	-0.6931	0.8268	0.2582	0.698	-0.015	-0.0592	1.3098	-0.1957	0.7741	44.3369	8.9578	71.8133
59	2.3094	0.3784	1.4292	1.0955	0.341	4.9176	-0.3602	-1.0151	0.5409	1.602	-0.8563	0.6584	-0.0468	-0.3023	0.7742	1.6526	0.321	-0.4324	2.2006	-0.6557	1.1757	45.327	19.3602	96.9874
60	3.4865	2.4787	0.1263	6.5109	2.8471	3.6207	-0.6749	-0.8899	1.1874	0.7893	0.1621	-0.4226	0.2633	0.3428	0.4535	1.5219	0.3287	-0.403	1.933	-0.5722	1.1762	51.9541	10.0399	93.2743
61	4.5656	1.4318	-1.2208	2.4206	-0.9357	2.7225	0.2636	-0.2235	0.3481	0.2562	0.0321	0.8792	-0.1159	-0.3467	-0.2884	0.4753	-0.0166	0.059	0.5051	-0.0609	0.6601	-39.7336	0.1619	93.4311
62	4.834	-1.8543	-0.0227	4.1371	-1.8328	5.1487	-0.0334	-1.5384	0.5216	1.7668	0.215	-0.0014	-0.1736	-0.3876	-0.145	1.3507	-0.0738	-0.127	1.6507	-0.1242	0.9206	-58.7095	-19.6864	118.4914
63	5.2792	-0.7717	2.5771	7.0359	-3.8246	6.9883	-0.0326	-0.1001	-0.7622	0.1191	0.0757	0.0535	0.8836	-0.2478	0.0332	0.6814	-0.0544	-0.0288	0.5337	-0.0234	0.7441	-31.9612	-25.1851	92.294
64	7.8192	-0.4842	-1.588	6.3961	-1.1439	4.5373	0.0418	-0.6404	-0.33	0.4579	0.0096	-0.0758	0.2724	0.1847	0.0784	0.4014	-0.0392	-0.0993	0.4051	-0.0473	0.4985	-26.0638	-15.9523	93.4888
65	7.4706	-0.335	1.1337	5.3346	-0.2482	0.5889	0.0136	-1.5051	-0.8428	0.9121	0.0881	0.0072	0.0173	-0.4234	0.203	0.6171	-0.0958	-0.0531	0.7333	0.0829	0.6226	-30.3539	-20.4932	100.3595
66	2.9554	2.1527	-2.4375	5.3096	-5.3544	6.8997	0.0469	-0.3761	-0.5473	0.085	0.0101	0.1656	0.6026	-0.339	-0.1427	0.6803	-0.1303	-0.0846	0.7396	0.0552	0.7033	-37.2186	-22.5429	96.2025
67	9.6559	0.1645	-0.9961	4.824	-0.9405	2.4465	-0.172	-0.6571	-0.5519	0.3947	0.191	0.2717	0.4216	-0.69	-0.2569	1.0389	-0.3021	-0.0812	1.2555	0.1684	0.6071	-61.5869	-31.1208	108.38
68	3.3882	1.7146	1.4042	6.405	3.3125	4.9587	-0.1944	0.7474	-0.2969	-0.867	0.5489	-0.6362	0.1357	0.5128	-0.3034	0.3865	0.0571	0.0176	0.567	-0.0163	0.3666	-22.8691	-24.6349	25.5813
69	3.2011	0.1209	1.0392	5.2559	2.0619	5.4806	0.1293	0.0732	-0.0552	-0.0588	0.1763	-0.4821	0.1226	0.2215	-0.2329	0.2767	0.0527	0.0226	0.3423	-0.0257	0.3146	-24.146	-18.6921	33.274
70	2.7599	-0.3939	0.0131	5.3702	0.3531	4.8574	0.2649	0.1752	-0.4602	-0.2638	0.0642	0.7421	0.4158	-0.6682	-0.0661	0.3079	-0.0269	-0.0533	0.4541	-0.0506	0.4271	-36.0193	-21.067	32.0498
71	6.9172	4.0167	4.2932	8.7513	4.0563	6.8078	0.3761	0.2165	-0.0993	0.6626	-0.0734	-0.2989	0.6138	0.501	-0.2412	0.3248	0.0225	0.0425	0.4747	-0.0157	0.275	-24.2515	-24.6845	36.1908
72	0.872	-2.0889	-0.3902	7.0754	-0.984	5.5263	0.042	0.0404	-0.0776	-0.6227	-0.0734	0.4601	0.5469	-0.2673	0.0312	0.3081	0.0425	-0.0364	0.4295	-0.0534	0.3358	-31.6374	-24.908	25.8668
73	1.9923	2.6327	2.4342	6.1604	5.0323	4.4149	0.0825	-0.0823	0.1008	0.0574	-0.0797	-0.6911	-0.2531	-0.1059	-0.1524	0.3922	-0.0038	-0.0066	0.3631	-0.0527	0.3803	-23.7017	-10.526	31.1563
74	3.0163	1.1609	1.9569	3.8654	4.6209	5.802	0.2945	0.0979	-0.0865	0.24	-0.0388	-0.3342	0.8257	-0.342	-0.3627	0.5331	0.1127	-0.079	0.4198	-0.0673	0.4271	-28.4363	-33.3619	11.3744
75	1.3829	0.6285	0.0136	2.914	-2.0388	2.2999	0.6553	0.7825	-0.353	-1.0418	-0.6032	-0.6705	-0.9963	-0.1101	0.1013	1.3616	0.5177	0.1681	0.909	-0.0154	0.2628	-27.9573	-21.8994	-8.1869
76	0.5249	-0.1896	0.7169	3.3326	-1.9343	2.9714	0.6757	0.7463	0.2583	-1.2452	-0.658	-0.1995	-0.4484	0.121	-0.1142	1.2065	0.4764	0.1149	0.9506	0.0832	0.4789	-26.92	-23.4433	-7.5124
77	1.0975	0.8423	1.0146	3.8639	1.6273	1.9329	0.4629	-1.3857	1.1139	0.938	-0.3717	-0.1549	-0.5647	0.0518	0.4623	0.9556	-0.1596	0.3997	0.4013	-0.515	1.2154	37.7072	19.0137	31.2042
78	2.7051	2.2249	0.4252	2.3113	0.5995	0.4446	0.3858	-0.3524	0.4281	0.3263	-0.3119	0.2401	-0.0207	-0.0465	-0.0528	0.4532	0.0081	0.0718	0.4623	-0.0462	0.4549	10.087	-16.8033	23.8696
79	1.989	1.8653	0.6133	3.947	0.1832	0.6371	0.2732	-0.0782	0.1186	-0.0766	-0.23	-0.1476	0.0657	-0.0983	-0.0943	0.3877	0.0772	0.0878	0.4292	-0.0268	0.3664	-1.5817	-33.2866	19.8656
80	2.6778	0.1893	0.259	3.2118	0.2759	0.9877	0.2004	0.2052	-0.079	-0.3751	-0.1728	0.1003	0.1326	-0.1655	-0.0186	0.4053	0.0828	0.0122	0.4469	-0.0304	0.3045	-9.5413	-44.2653	9.3925
81	3.6993	-0.3932	-1.6202	3.6094	-0.2452	4.7733	0.2602	0.1034	-0.1024	-0.686	-0.2187	-0.313	0.4866	1.0773	0.0389	0.4696	0.0906	0.0214	0.5622	-0.0394	0.4173	-1.5017	-52.1479	9.5757
82	2.5059	0.7449	0.6131	4.118	-0.6244	1.3088	0.1197	0.4438	0.0387	-0.5333	-0.1762	-0.1003	0.1318	0.0664	0.0169	0.4637	0.1366	0.0917	0.5416	-0.0221	0.3309	-4.243	-39.0431	4.3361
83	3.0821	1.6491	0.0586	1.3676	-0.6707	0.6139	0.126	-0.1963	0.5362	-0.004	-0.1024	0.3173	-0.0273	0.0266	0.0141	0.4311	0.0428	0.1001	0.405	-0.0733	0.4891	10.2699	-15.4564	23.4099
84	3.0025	1.5422	0.4977	4.6689	1.2978	2.1018	0.0854	-0.0022	0.104	0.3031	0.3468	-0.3836	-0.362	0.2475	-0.3286	0.5011	0.0063	0.1497	0.3971	-0.0684	0.4905	26.2274	-4.8179	24.3481
85	2.0814	2.7385	2.3201	5.8385	2.6956	3.262	0.4111	-0.2389	0.7902	0.6206	-0.1893	0.2202	-0.3506	0.2449	-0.2582	0.8221	-0.0556	0.3691	0.6473	-0.2224	0.9718	32.3395	18.9176	26.1477
86	0.9912	0.1164	-0.0829	1.1358	0.45	1.0913	0.2125	-0.0712	0.6872	0.1809	0.023	0.2927	-0.4887	0.1496	-0.105	0.6009	-0.0369	0.2217	0.4983	-0.1162	0.7407	34.1672	8.8058	18.4964
87	8.1078	5.931	-0.5931	4.1605	0.1131	3.6445	-0.3988	0.5632	1.0412	-0.6139	0.6856	0.4459	-0.7412	0.4801	-0.2073	0.7833	-0.0313	0.1749	0.5897	0.0462	1.0495	38.0766	23.0911	-3.2239
88	0.8699	0.7839	-0.2037	3.5999	0.6275	1.4566	-0.0947	0.3589	0.0448	-0.4461	-0.0203	0.1592	0.239	-0.4346	0.1309	0.4471	-0.0543	0.0701	0.6914	-0.0304	0.3838	19.1647	-34.6924	-16.538
89	5.6031	0.5553	-0.2086	2.0205	0.0889	2.4309	-0.0034	0.0062	-0.5447	-0.2198	0.0505	0.0495	0.7895	-0.7137	-0.0844	0.8249	-0.3451	0.1083	0.7041	-0.0967	0.4369	15.1322	-59.8496	-13.1904
90	3.0708	-0.2645	0.9133	2.5397	-0.4482	3.626	-0.0648	-0.2445	-0.0505	0.0419	0.0275	0.3641	0.5823	-0.7857	0.0267	0.6216	-0.2509	0.1282	0.7043	-0.1488	0.4459	13.4837	-55.4182	-7.6247
91	0.495	0.4517	-0.0603	2.4674	0.3498	1.4013	-0.0942	0.0886	0.0609	-0.4024	0.1478	-0.1185	-0.023	-0.0406	-0.0243	0.412	-0.0567	0.0507	0.5954	-0.0556	0.377	24.296	-27.6275	-13.9177
92	2.7552	2.5541	-0.1158	6.8997	0.6062	1.8981	-0.1005	0.3795	0.6106	0.353	0.4286	0.6344	-0.2284	0.117	-0.3805	0.4807	0.0057	0.0944	0.5421	0.0511	0.4964	27.8777	-2.288	-3.4337
93	5.0203	1.8032	1.3164	3.8102	-0.8054	5.2795	-0.2649	-0.0824	1.008	-0.7069	0.195	0.558	-2.2671	-0.6686	-0.1814	1.1179	0.1006	0.1208	0.5359	0.0905	1.1244	38.1259	21.1839	-4.7401
94	2.9896	1.845	-0.1783	3.7347	0.0468	1.5491	0.137	0.1163	0.8492	-0.5371	0.0876	-0.1356	-0.7262	0.1819	-0.2088	0.7625	-0.0882	0.2707	0.49	-0.0316	0.7669	44.7887	14.6624	-6.032
95	9.9599	2.1844	3.3052	2.9687	3.7699	4.8861	0.6014	0.2351	-1.1198	0.2809	0.1094	-0.4195	0.0988	0.4851	-0.4013	0.5964	0.0568	0.0872	0.8026	-0.0108	0.6193	37.0562	-17.1622	-5.2172
96	2.1085	-0.8082	-0.3928	1.0829	-0.13	2.9238	-0.0506	0.4826	-0.3584	-0.0651	0.164	0.2711	0.1271	-0.3218	-0.1068	0.5251	-0.0133	-0.0345	0.6259	0.0213	0.4064	18.0103	-28.8193	-3.3207
97	3.5666	4.0343	-0.6659	6.6738	-0.5644	0.8154	0.2907	-0.1996	0.6644	0.0102	-0.0973	0.0297	-0.5801	0.2308	-0.016	0.7215	-0.1218	0.2315	0.5066	-0.1158	0.7633	43.6231	13.7599	3.6699
98	1.4059	1.8593	-0.773	4.5293	-1.1136	0.632	0.0113	-0.1212	-0.217	-0.0471	-0.0422	-0.7246	-0.087	0.1551	0.0326	0.3269	-0.0032	0.0178	0.5362	-0.0712	0.4877	47.647	-27.872	15.7549
99	1.5186	0.7433	-0.5471	2.1549	-0.1878	3.3211	-0.2581	0.0151	-0.2323	-0.077	0.1006	-0.1659	0.3166	-0.4129	0.163	0.3187	-0.0023	0.0561	0.4691	-0.068	0.3933	21.7037	-58.9387	19.0898
100	0.8793	1.1077	-0.0079	5.2231	-0.0717	1.2646	0.0499	0.0078	-0.082	-0.0023	-0.0544	-0.3695	-0.0908	0.1801	-0.0277	0.2867	0.0248	0.0388	0.506	-0.0453	0.4909	47.8764	-23.5735	13.2692
101	5.897	4.5024	-0.0454	3.7491	-0.2885	4.382	-0.2681	0.609	-0.2576	-0.9416	0.3552	-1.6369	-0.2194	0.8922	-0.2351	0.6612	-0.1129	0.1628	0.6463	-0.2202	0.954	64.0043	6.4301	13.5873
102	3.8158	2.2912	0.649	4.0499	1.2924	1.4711	0.1794	-0.2548	0.3217	0.1412	-0.1486	-0.3224	-0.1705	0.2886	-0.0335	0.3678	0.008	-0.0258	0.4868	-0.0494	0.5003	52.8374	-4.3915	21.3745
103	6.6443	-0.826	5.2974	5.2462	2.6543	8.1565	0.0017	1.0348	-0.5883	0.4323	0.1979	0.0123	0.9246	0.7429	-0.2099	0.4454	0.0535	0.0262	0.5999	-0.0586	0.4949	30.9536	-40.223	3.5515
104	4.3069	4.0418	1.321	5.0074	1.7317	1.7972	0.0142	0.0557	0.4324	-0.0226	-0.0102	0.0793	-0.3146	0.142	-0.0441	0.3842	0.009	0.0848	0.4199	-0.0136	0.4406	43.3438	-4.1342	11.7756
105	0.5943	-0.283	0.8527	2.4133	0.6475	2.1771	0.1504	-0.276	0.5178	0.1449	-0.2483	-0.3081	-0.8956	0.4978	0.2827	1.1971	-0.4202	0.2412	0.8175	-0.2811	1.1995	65.4873	25.087	22.9346
106	3.6411	-1.1109	1.0272	2.6983	-0.9657	2.3292	-0.0075	0.1857	1.1349	-0.0913	0.193	0.0067	-0.6852	-0.1809	0.0176	0.7786	0.088	0.3421	0.5589	0.1323	1.2895	8.3688	25.1839	10.3727
107	8.8761	2.4961	-2.4364	4.7006	-2.7251	5.2473	-0.263	1.2563	1.8125	-1.0344	0.4799	0.6979	0.2395	-1.931	-0.0801	1.1409	0.2672	-0.1306	1.5197	0.1857	1.6553	-34.1222	29.796	-6.972
108	1.2956	-0.4091	1.5377	5.8406	-1.3994	3.51	0.1486	-1.107	1.0651	0.1255	-0.5785	0.6254	-0.6504	-0.4395	0.3721	1.1299	0.2226	0.2049	1.1869	0.0861	1.5058	-24.7954	32.6399	0.4914
109	2.0498	-0.2994	-3.7592	2.0154	-1.0013	8.4508	0.3002	0.3646	0.4853	-0.5097	-0.0555	0.1919	0.2685	-0.5188	-0.3978	0.7151	0.1898	0.1264	0.5999	0.1445	0.8263	-0.6184	5.0923	0.3349
110	4.9024	2.2807	1.3763	3.3774	-0.2668	2.6183	0.2744	-0.1623	0.547	0.2328	0.0971	0.3105	0.1476	-0.5171	-0.2227	0.5752	0.065	0.2109	0.534	0.1527	0.6755	0.1222	4.1797	11.9246
111	3.2578	4.7698	0.3774	8.3048	-1.6382	4.223	0.3333	-1.0973	0.8478	-0.3321	-0.0066	0.6821	-0.2478	-0.4855	-0.2622	0.5848	0.1344	0.2434	0.6943	0.1441	0.8535	-7.3448	7.3084	10.7978
112	8.4387	0.4372	3.5441	0.6672	1.4579	3.2467	-0.2977	-0.0364	-0.1568	-0.4562	0.2663	-0.1455	-0.2268	-0.3617	0.0527	0.7406	0.1289	0.2698	0.8124	0.3366	0.8779	-4.68	9.2092	2.6744
113	3.4728	1.9296	-0.1211	4.8276	-4.8045	7.4588	-0.4588	0.6968	0.9965	-1.7185	0.2122	0.2436	0.0279	-0.389	0.5781	1.6268	0.2024	0.1331	1.191	0.3379	1.1104	-23.2877	24.4343	-11.6615
114	5.7164	0.5361	0.3256	3.6396	0.8489	0.2069	-0.7983	0.7903	1.5377	-1.8607	-0.2481	0.4862	0.0975	-0.4215	0.5189	2.5109	0.1009	-0.2105	1.414	0.3905	1.0365	-30.1327	21.7945	-18.7266
115	2.7887	-0.983	-2.3568	-0.2876	0.709	2.1574	0.213	0.0372	0.597	-0.115	0.206	-0.2526	-0.4309	0.1848	-0.4288	0.7556	-0.2041	0.2459	0.5728	-0.1148	0.7557	61.2158	0.6903	-20.9523
116	2.8622	2.0385	2.2586	2.1286	2.4289	3.1416	0.3808	0.3398	-0.7921	0.3713	0.5766	-0.6695	1.0868	0.9461	-0.7996	0.7798	0.0614	-0.0997	0.6112	-0.0551	0.5925	81.853	-28.5335	-50.7554
117	1.9401	1.7133	0.847	4.4301	1.7021	4.6163	0.0791	-0.0647	0.2183	0.0411	-0.1584	0.1354	0.1362	0.0955	0.0035	0.4171	-0.0435	0.0575	0.3925	0.0386	0.4064	77.5585	-17.5075	-47.7287
118	4.5997	0.3075	3.4745	3.9507	3.1657	8.084	0.1571	-0.1854	-0.5443	0.0784	0.4046	-0.4804	0.5529	0.1954	-0.1653	0.5841	-0.1193	0.178	0.5827	-0.0251	0.5093	52.7704	-24.336	-22.0309
119	4.5336	0.4788	-0.5035	4.7463	2.4773	3.6432	-0.0359	0.2746	0.0675	-0.3539	0.118	-0.1387	-0.1742	-0.0739	-0.0365	0.4712	-0.0876	0.1038	0.4604	-0.0017	0.4558	46.845	-15.5335	-21.1939
120	3.3913	0.137	1.1873	4.7804	2.643	4.9653	-0.0583	0.633	-0.1265	-0.3451	0.0839	0.2134	-0.2193	-0.3469	0.2447	0.5167	-0.1193	0.1697	0.6515	-0.0396	0.5103	50.5627	-18.9376	-28.7993
121	1.8182	-3.3037	-3.9013	4.6074	5.221	6.9828	0.0276	-0.0037	0.306	0.2919	0.1137	-0.2837	0.3605	-0.1972	-0.041	0.4987	-0.1623	0.1297	0.4885	-0.0331	0.5139	57.1853	-20.7976	-23.6269
122	6.2616	1.2581	-1.1855	5.8992	-0.3224	2.3996	0.4908	-0.3185	0.2301	0.391	-0.2618	-0.5336	0.0762	0.2536	-0.2592	0.5957	-0.0783	-0.0346	0.4218	-0.002	0.4128	80.569	-29.0413	-39.8058
123	4.3465	-4.1804	1.4792	7.0213	-0.2238	4.0939	0.4596	-0.6909	-0.2321	1.295	-0.0582	-0.5193	1.118	0.2185	-0.2979	1.124	-0.1874	-0.1489	0.6665	0.0188	0.4432	83.9196	-30.3813	-31.3112
124	9.0871	1.4584	-0.5741	3.8973	-0.3099	2.7553	0.2786	0.1392	-0.0863	0.2444	-0.231	-0.6565	0.3328	0.2392	-0.0171	0.6209	-0.0351	-0.0806	0.4743	0.022	0.3663	80.5596	-32.2765	-39.1212
125	1.2726	0.3593	-0.3053	0.6046	-0.432	4.076	0.113	-0.3201	0.2291	0.1885	-0.1501	0.0308	-0.0057	-0.0592	0.04	0.3652	-0.0703	0.0256	0.5348	-0.1431	0.5573	56.4705	-7.8868	49.2907
126	2.0054	-0.3868	-0.243	7.0728	-0.4199	2.9932	0.1864	-0.4924	-0.2032	0.5034	-0.2507	-0.694	-0.2453	0.6325	0.0617	0.5759	-0.1142	-0.1074	1.1581	-0.0252	0.6575	61.9443	-24.1626	74.7572
127	4.3568	-2.6679	1.2121	9.279	-2.988	2.0571	0.1119	-0.3797	-0.3804	-0.0281	0.186	0.3595	0.224	-0.4425	-0.3579	0.4281	-0.1255	-0.0598	0.7105	-0.0626	0.5399	41.6394	-27.524	43.9406
128	1.6876	-0.6982	-0.8459	6.8015	0.2847	5.354	0.1301	-0.0506	-0.0922	0.0724	-0.0643	-0.2314	0.2735	0.3438	0.0099	0.2681	-0.0716	0.0086	0.5051	-0.0688	0.4453	49.5837	-23.6206	37.4468
129	1.2421	0.5676	-0.4242	1.4543	-1.18	2.3505	0.2033	-0.3484	0.0404	0.1399	-0.0757	0.0127	0.0751	0.4328	-0.0688	0.3806	-0.0319	-0.0515	0.6231	-0.1106	0.549	49.6568	-25.8719	58.0639
130	4.4697	-0.5903	-1.7724	8.0821	6.9812	7.9707	0.1866	-0.2202	-0.262	0.3889	-0.2087	0.3674	-0.273	0.0449	0.0298	0.5357	-0.0748	-0.0576	0.6734	0.0507	0.4977	45.0913	-30.5949	74.0364
131	1.6579	1.478	0.6702	4.2861	1.3979	5.8284	0.3801	-0.7655	0.1566	0.5883	-0.331	0.0481	-0.036	-0.0427	-0.1099	0.521	-0.0375	-0.0562	0.9903	-0.0461	0.5661	45.2814	-29.4389	79.1023

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	( CHAIN A AND RESID 8:26 )	A	8 - 26
2	X-RAY DIFFRACTION	2	( CHAIN A AND RESID 27:79 )	A	27 - 79
3	X-RAY DIFFRACTION	3	( CHAIN A AND RESID 80:175 )	A	80 - 175
4	X-RAY DIFFRACTION	4	( CHAIN A AND RESID 176:228 )	A	176 - 228
5	X-RAY DIFFRACTION	5	( CHAIN A AND RESID 229:265 )	A	229 - 265
6	X-RAY DIFFRACTION	6	( CHAIN A AND RESID 266:300 )	A	266 - 300
7	X-RAY DIFFRACTION	7	( CHAIN A AND RESID 301:325 )	A	301 - 325
8	X-RAY DIFFRACTION	8	( CHAIN A AND RESID 326:327 )	A	326 - 327
9	X-RAY DIFFRACTION	9	( CHAIN B AND RESID 4:21 )	B	4 - 21
10	X-RAY DIFFRACTION	10	( CHAIN B AND RESID 22:65 )	B	22 - 65
11	X-RAY DIFFRACTION	11	( CHAIN B AND RESID 66:75 )	B	66 - 75
12	X-RAY DIFFRACTION	12	( CHAIN B AND RESID 76:132 )	B	76 - 132
13	X-RAY DIFFRACTION	13	( CHAIN B AND RESID 133:171 )	B	133 - 171
14	X-RAY DIFFRACTION	14	( CHAIN C AND RESID 8:104 )	C	8 - 104
15	X-RAY DIFFRACTION	15	( CHAIN C AND RESID 105:288 )	C	105 - 288
16	X-RAY DIFFRACTION	16	( CHAIN C AND RESID 289:326 )	C	289 - 326
17	X-RAY DIFFRACTION	17	( CHAIN D AND RESID 3:22 )	D	3 - 22
18	X-RAY DIFFRACTION	18	( CHAIN D AND RESID 23:56 )	D	23 - 56
19	X-RAY DIFFRACTION	19	( CHAIN D AND RESID 57:75 )	D	57 - 75
20	X-RAY DIFFRACTION	20	( CHAIN D AND RESID 76:132 )	D	76 - 132
21	X-RAY DIFFRACTION	21	( CHAIN D AND RESID 133:171 )	D	133 - 171
22	X-RAY DIFFRACTION	22	( CHAIN E AND RESID 8:26 )	E	8 - 26
23	X-RAY DIFFRACTION	23	( CHAIN E AND RESID 27:146 )	E	27 - 146
24	X-RAY DIFFRACTION	24	( CHAIN E AND RESID 147:175 )	E	147 - 175
25	X-RAY DIFFRACTION	25	( CHAIN E AND RESID 176:249 )	E	176 - 249
26	X-RAY DIFFRACTION	26	( CHAIN E AND RESID 250:300 )	E	250 - 300
27	X-RAY DIFFRACTION	27	( CHAIN E AND RESID 301:325 )	E	301 - 325
28	X-RAY DIFFRACTION	28	( CHAIN F AND RESID 5:56 )	F	5 - 56
29	X-RAY DIFFRACTION	29	( CHAIN F AND RESID 57:75 )	F	57 - 75
30	X-RAY DIFFRACTION	30	( CHAIN F AND RESID 76:145 )	F	76 - 145
31	X-RAY DIFFRACTION	31	( CHAIN F AND RESID 146:172 )	F	146 - 172
32	X-RAY DIFFRACTION	32	( CHAIN G AND RESID 1:17 )	G	1 - 17
33	X-RAY DIFFRACTION	33	( CHAIN G AND RESID 18:52 )	G	18 - 52
34	X-RAY DIFFRACTION	34	( CHAIN G AND RESID 53:64 )	G	53 - 64
35	X-RAY DIFFRACTION	35	( CHAIN G AND RESID 65:83 )	G	65 - 83
36	X-RAY DIFFRACTION	36	( CHAIN G AND RESID 84:99 )	G	84 - 99
37	X-RAY DIFFRACTION	37	( CHAIN G AND RESID 100:121 )	G	100 - 121
38	X-RAY DIFFRACTION	38	( CHAIN G AND RESID 122:134 )	G	122 - 134
39	X-RAY DIFFRACTION	39	( CHAIN G AND RESID 135:149 )	G	135 - 149
40	X-RAY DIFFRACTION	40	( CHAIN G AND RESID 150:192 )	G	150 - 192
41	X-RAY DIFFRACTION	41	( CHAIN G AND RESID 193:231 )	G	193 - 231
42	X-RAY DIFFRACTION	42	( CHAIN H AND RESID 1:44 )	H	1 - 44
43	X-RAY DIFFRACTION	43	( CHAIN H AND RESID 45:64 )	H	45 - 64
44	X-RAY DIFFRACTION	44	( CHAIN H AND RESID 65:83 )	H	65 - 83
45	X-RAY DIFFRACTION	45	( CHAIN H AND RESID 84:99 )	H	84 - 99
46	X-RAY DIFFRACTION	46	( CHAIN H AND RESID 100:121 )	H	100 - 121
47	X-RAY DIFFRACTION	47	( CHAIN H AND RESID 122:134 )	H	122 - 134
48	X-RAY DIFFRACTION	48	( CHAIN H AND RESID 135:149 )	H	135 - 149
49	X-RAY DIFFRACTION	49	( CHAIN H AND RESID 150:231 )	H	150 - 231
50	X-RAY DIFFRACTION	50	( CHAIN I AND RESID 1:18 )	I	1 - 18
51	X-RAY DIFFRACTION	51	( CHAIN I AND RESID 19:38 )	I	19 - 38
52	X-RAY DIFFRACTION	52	( CHAIN I AND RESID 39:75 )	I	39 - 75
53	X-RAY DIFFRACTION	53	( CHAIN I AND RESID 76:90 )	I	76 - 90
54	X-RAY DIFFRACTION	54	( CHAIN I AND RESID 91:102 )	I	91 - 102
55	X-RAY DIFFRACTION	55	( CHAIN I AND RESID 103:113 )	I	103 - 113
56	X-RAY DIFFRACTION	56	( CHAIN I AND RESID 114:150 )	I	114 - 150
57	X-RAY DIFFRACTION	57	( CHAIN I AND RESID 151:163 )	I	151 - 163
58	X-RAY DIFFRACTION	58	( CHAIN I AND RESID 164:174 )	I	164 - 174
59	X-RAY DIFFRACTION	59	( CHAIN I AND RESID 175:197 )	I	175 - 197
60	X-RAY DIFFRACTION	60	( CHAIN I AND RESID 198:214 )	I	198 - 214
61	X-RAY DIFFRACTION	61	( CHAIN J AND RESID 1:134 )	J	1 - 134
62	X-RAY DIFFRACTION	62	( CHAIN J AND RESID 135:231 )	J	135 - 231
63	X-RAY DIFFRACTION	63	( CHAIN K AND RESID 1:25 )	K	1 - 25
64	X-RAY DIFFRACTION	64	( CHAIN K AND RESID 26:75 )	K	26 - 75
65	X-RAY DIFFRACTION	65	( CHAIN K AND RESID 76:90 )	K	76 - 90
66	X-RAY DIFFRACTION	66	( CHAIN K AND RESID 91:113 )	K	91 - 113
67	X-RAY DIFFRACTION	67	( CHAIN K AND RESID 114:214 )	K	114 - 214
68	X-RAY DIFFRACTION	68	( CHAIN L AND RESID 1:18 )	L	1 - 18
69	X-RAY DIFFRACTION	69	( CHAIN L AND RESID 19:38 )	L	19 - 38
70	X-RAY DIFFRACTION	70	( CHAIN L AND RESID 39:61 )	L	39 - 61
71	X-RAY DIFFRACTION	71	( CHAIN L AND RESID 62:75 )	L	62 - 75
72	X-RAY DIFFRACTION	72	( CHAIN L AND RESID 76:90 )	L	76 - 90
73	X-RAY DIFFRACTION	73	( CHAIN L AND RESID 91:102 )	L	91 - 102
74	X-RAY DIFFRACTION	74	( CHAIN L AND RESID 103:113 )	L	103 - 113
75	X-RAY DIFFRACTION	75	( CHAIN L AND RESID 114:155 )	L	114 - 155
76	X-RAY DIFFRACTION	76	( CHAIN L AND RESID 156:214 )	L	156 - 214
77	X-RAY DIFFRACTION	77	( CHAIN M AND RESID 8:26 )	M	8 - 26
78	X-RAY DIFFRACTION	78	( CHAIN M AND RESID 27:79 )	M	27 - 79
79	X-RAY DIFFRACTION	79	( CHAIN M AND RESID 80:104 )	M	80 - 104
80	X-RAY DIFFRACTION	80	( CHAIN M AND RESID 105:195 )	M	105 - 195
81	X-RAY DIFFRACTION	81	( CHAIN M AND RESID 196:228 )	M	196 - 228
82	X-RAY DIFFRACTION	82	( CHAIN M AND RESID 229:265 )	M	229 - 265
83	X-RAY DIFFRACTION	83	( CHAIN M AND RESID 266:300 )	M	266 - 300
84	X-RAY DIFFRACTION	84	( CHAIN M AND RESID 301:325 )	M	301 - 325
85	X-RAY DIFFRACTION	85	( CHAIN N AND RESID 4:56 )	N	4 - 56
86	X-RAY DIFFRACTION	86	( CHAIN N AND RESID 57:172 )	N	57 - 172
87	X-RAY DIFFRACTION	87	( CHAIN O AND RESID 8:26 )	O	8 - 26
88	X-RAY DIFFRACTION	88	( CHAIN O AND RESID 27:153 )	O	27 - 153
89	X-RAY DIFFRACTION	89	( CHAIN O AND RESID 154:195 )	O	154 - 195
90	X-RAY DIFFRACTION	90	( CHAIN O AND RESID 196:249 )	O	196 - 249
91	X-RAY DIFFRACTION	91	( CHAIN O AND RESID 250:300 )	O	250 - 300
92	X-RAY DIFFRACTION	92	( CHAIN O AND RESID 301:325 )	O	301 - 325
93	X-RAY DIFFRACTION	93	( CHAIN P AND RESID 9:21 )	P	9 - 21
94	X-RAY DIFFRACTION	94	( CHAIN P AND RESID 22:56 )	P	22 - 56
95	X-RAY DIFFRACTION	95	( CHAIN P AND RESID 57:61 )	P	57 - 61
96	X-RAY DIFFRACTION	96	( CHAIN P AND RESID 62:75 )	P	62 - 75
97	X-RAY DIFFRACTION	97	( CHAIN P AND RESID 76:171 )	P	76 - 171
98	X-RAY DIFFRACTION	98	( CHAIN Q AND RESID 9:89 )	Q	9 - 89
99	X-RAY DIFFRACTION	99	( CHAIN Q AND RESID 90:269 )	Q	90 - 269
100	X-RAY DIFFRACTION	100	( CHAIN Q AND RESID 270:326 )	Q	270 - 326
101	X-RAY DIFFRACTION	101	( CHAIN R AND RESID 5:22 )	R	5 - 22
102	X-RAY DIFFRACTION	102	( CHAIN R AND RESID 23:66 )	R	23 - 66
103	X-RAY DIFFRACTION	103	( CHAIN R AND RESID 67:75 )	R	67 - 75
104	X-RAY DIFFRACTION	104	( CHAIN R AND RESID 76:132 )	R	76 - 132
105	X-RAY DIFFRACTION	105	( CHAIN R AND RESID 133:171 )	R	133 - 171
106	X-RAY DIFFRACTION	106	( CHAIN S AND RESID 1:134 )	S	1 - 134
107	X-RAY DIFFRACTION	107	( CHAIN S AND RESID 135:149 )	S	135 - 149
108	X-RAY DIFFRACTION	108	( CHAIN S AND RESID 150:231 )	S	150 - 231
109	X-RAY DIFFRACTION	109	( CHAIN T AND RESID 1:13 )	T	1 - 13
110	X-RAY DIFFRACTION	110	( CHAIN T AND RESID 14:75 )	T	14 - 75
111	X-RAY DIFFRACTION	111	( CHAIN T AND RESID 76:90 )	T	76 - 90
112	X-RAY DIFFRACTION	112	( CHAIN T AND RESID 91:115 )	T	91 - 115
113	X-RAY DIFFRACTION	113	( CHAIN T AND RESID 116:189 )	T	116 - 189
114	X-RAY DIFFRACTION	114	( CHAIN T AND RESID 190:214 )	T	190 - 214
115	X-RAY DIFFRACTION	115	( CHAIN U AND RESID 1:134 )	U	1 - 134
116	X-RAY DIFFRACTION	116	( CHAIN U AND RESID 135:149 )	U	135 - 149
117	X-RAY DIFFRACTION	117	( CHAIN U AND RESID 150:231 )	U	150 - 231
118	X-RAY DIFFRACTION	118	( CHAIN V AND RESID 1:18 )	V	1 - 18
119	X-RAY DIFFRACTION	119	( CHAIN V AND RESID 19:75 )	V	19 - 75
120	X-RAY DIFFRACTION	120	( CHAIN V AND RESID 76:90 )	V	76 - 90
121	X-RAY DIFFRACTION	121	( CHAIN V AND RESID 91:115 )	V	91 - 115
122	X-RAY DIFFRACTION	122	( CHAIN V AND RESID 116:152 )	V	116 - 152
123	X-RAY DIFFRACTION	123	( CHAIN V AND RESID 153:165 )	V	153 - 165
124	X-RAY DIFFRACTION	124	( CHAIN V AND RESID 166:214 )	V	166 - 214
125	X-RAY DIFFRACTION	125	( CHAIN X AND RESID 1:149 )	X	1 - 149
126	X-RAY DIFFRACTION	126	( CHAIN X AND RESID 150:231 )	X	150 - 231
127	X-RAY DIFFRACTION	127	( CHAIN Y AND RESID 1:25 )	Y	1 - 25
128	X-RAY DIFFRACTION	128	( CHAIN Y AND RESID 26:75 )	Y	26 - 75
129	X-RAY DIFFRACTION	129	( CHAIN Y AND RESID 76:130 )	Y	76 - 130
130	X-RAY DIFFRACTION	130	( CHAIN Y AND RESID 131:152 )	Y	131 - 152
131	X-RAY DIFFRACTION	131	( CHAIN Y AND RESID 153:214 )	Y	153 - 214

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