温度: 293 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 7 詳細: 12% PEG-3350, 0.1M DL-Malic acid. 0.001 M Bromosporine was also added but not located in the electron density.
構造決定の手法: 単波長異常分散 / 解像度: 2.1→49.41 Å / Cor.coef. Fo:Fc: 0.953 / Cor.coef. Fo:Fc free: 0.947 / Rfactor Rfree error: 0 / SU R Cruickshank DPI: 0.173 / 交差検証法: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.169 / SU Rfree Blow DPI: 0.15 / SU Rfree Cruickshank DPI: 0.153 詳細: The structure was solved by SAD with a nearly isomorphous selenomethionyl derivative and the CCP4 SHELX-PHASER-PARROT-BUCCANEER pipeline. ARP/WARP in atom update and autobuild modes with the ...詳細: The structure was solved by SAD with a nearly isomorphous selenomethionyl derivative and the CCP4 SHELX-PHASER-PARROT-BUCCANEER pipeline. ARP/WARP in atom update and autobuild modes with the current native data gave a model that was further refined with REFMAC and BUSTER.