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Yorodumi- PDB-5ix2: Crystal structure of mouse Morc3 ATPase-CW cassette in complex wi... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5ix2 | ||||||
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Title | Crystal structure of mouse Morc3 ATPase-CW cassette in complex with AMPPNP and unmodified H3 peptide | ||||||
Components |
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Keywords | TRANSCRIPTION / Morc3 / ATPase / CW domain / H3 | ||||||
Function / homology | Function and homology information Chromatin modifying enzymes / Interleukin-7 signaling / HDMs demethylate histones / PRC2 methylates histones and DNA / HATs acetylate histones / PKMTs methylate histone lysines / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / RMTs methylate histone arginines / Factors involved in megakaryocyte development and platelet production / Estrogen-dependent gene expression ...Chromatin modifying enzymes / Interleukin-7 signaling / HDMs demethylate histones / PRC2 methylates histones and DNA / HATs acetylate histones / PKMTs methylate histone lysines / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / RMTs methylate histone arginines / Factors involved in megakaryocyte development and platelet production / Estrogen-dependent gene expression / negative regulation of interferon-beta production / maintenance of protein location in nucleus / antiviral innate immune response / negative regulation of fibroblast proliferation / methylated histone binding / post-embryonic development / PML body / nuclear matrix / structural constituent of chromatin / positive regulation of cellular senescence / nucleosome / protein-macromolecule adaptor activity / chromatin organization / peptidyl-serine phosphorylation / protein stabilization / protein heterodimerization activity / protein phosphorylation / chromatin / negative regulation of transcription by RNA polymerase II / ATP hydrolysis activity / protein-containing complex / DNA binding / RNA binding / zinc ion binding / nucleoplasm / nucleus Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.9 Å | ||||||
Authors | Li, S. / Du, J. / Patel, D.J. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2016 Title: Mouse MORC3 is a GHKL ATPase that localizes to H3K4me3 marked chromatin Authors: Li, S. / Yen, L. / Pastor, W.A. / Johnston, J.B. / Du, J. / Shew, C.J. / Liu, W. / Ho, J. / Stender, B. / Clark, A.T. / Burlingame, A.L. / Daxinger, L. / Patel, D.J. / Jacobsen, S.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5ix2.cif.gz | 365.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5ix2.ent.gz | 301.5 KB | Display | PDB format |
PDBx/mmJSON format | 5ix2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ix/5ix2 ftp://data.pdbj.org/pub/pdb/validation_reports/ix/5ix2 | HTTPS FTP |
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-Related structure data
Related structure data | 5ix1SC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 51950.477 Da / Num. of mol.: 2 / Fragment: UNP residues 7-456 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Morc3 / Plasmid: pET-Sumo / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) RIL / References: UniProt: F7BJB9 #2: Protein/peptide | Mass: 3347.915 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Mus musculus (house mouse) / References: UniProt: P68433 #3: Chemical | #4: Chemical | #5: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.25 Å3/Da / Density % sol: 62.13 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 5% ethanol, 5% 2-Methyl-2,4-pentanediol (MPD), 0.1 M HEPES-Na |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.9792 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Mar 19, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→20 Å / Num. obs: 32131 / % possible obs: 99.6 % / Redundancy: 6.3 % / Rmerge(I) obs: 0.091 / Net I/σ(I): 12.5 |
Reflection shell | Resolution: 2.9→3.06 Å / Redundancy: 6.9 % / Rmerge(I) obs: 0.701 / Mean I/σ(I) obs: 2.6 / % possible all: 99.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5IX1 Resolution: 2.9→19.754 Å / SU ML: 0.38 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 30.56 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.9→19.754 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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