Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/V66D at cryogenic temperature
要素
Thermonuclease
キーワード
HYDROLASE / nuclease / hyperstable / pdTp / ionizable group
機能・相同性
機能・相同性情報
endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters / micrococcal nuclease / nucleic acid binding / extracellular region / membrane / metal ion binding / cytoplasm 類似検索 - 分子機能
Thermonuclease family signature 1. / OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #90 / Thermonuclease active site / Thermonuclease family signature 2. / Staphylococcal nuclease (SNase-like), OB-fold / Staphylococcal nuclease homologue / Thermonuclease domain profile. / Staphylococcal nuclease homologues / SNase-like, OB-fold superfamily / OB fold (Dihydrolipoamide Acetyltransferase, E2P) ...Thermonuclease family signature 1. / OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #90 / Thermonuclease active site / Thermonuclease family signature 2. / Staphylococcal nuclease (SNase-like), OB-fold / Staphylococcal nuclease homologue / Thermonuclease domain profile. / Staphylococcal nuclease homologues / SNase-like, OB-fold superfamily / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Beta Barrel / Mainly Beta 類似検索 - ドメイン・相同性
解像度: 1.7→38.32 Å / Cor.coef. Fo:Fc: 0.954 / Cor.coef. Fo:Fc free: 0.947 / SU B: 2.105 / SU ML: 0.071 / SU R Cruickshank DPI: 0.1165 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.117 / ESU R Free: 0.108 / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS