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- PDB-5i9e: Crystal structure of a nuclear actin ternary complex -

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Basic information

Entry
Database: PDB / ID: 5i9e
TitleCrystal structure of a nuclear actin ternary complex
Components
  • Actin
  • Actin-related protein 4
  • Helicase SWR1
KeywordsHYDROLASE / nuclear actin / Arp4 / chromatin remodeling
Function / homology
Function and homology information


Swr1 complex / kinetochore assembly / Ino80 complex / SWI/SNF complex / recombinational repair / NuA4 histone acetyltransferase complex / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / chromatin organization / molecular adaptor activity / DNA helicase ...Swr1 complex / kinetochore assembly / Ino80 complex / SWI/SNF complex / recombinational repair / NuA4 histone acetyltransferase complex / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / chromatin organization / molecular adaptor activity / DNA helicase / histone binding / forked DNA-dependent helicase activity / single-stranded 3'-5' DNA helicase activity / four-way junction helicase activity / double-stranded DNA helicase activity / cytoskeleton / hydrolase activity / chromatin remodeling / DNA repair / DNA-templated transcription / chromatin binding / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / chromatin / structural molecule activity / ATP hydrolysis activity / DNA binding / ATP binding / nucleus / cytosol / cytoplasm
Similarity search - Function
: / domain in helicases and associated with SANT domains / HSA domain / Helicase/SANT-associated domain / HSA domain profile. / ATPase, substrate binding domain, subdomain 4 / : / SNF2-like, N-terminal domain superfamily / SNF2, N-terminal / SNF2-related domain ...: / domain in helicases and associated with SANT domains / HSA domain / Helicase/SANT-associated domain / HSA domain profile. / ATPase, substrate binding domain, subdomain 4 / : / SNF2-like, N-terminal domain superfamily / SNF2, N-terminal / SNF2-related domain / Actin; Chain A, domain 4 / ATPase, nucleotide binding domain / Actins signature 1. / Actin, conserved site / Actins signature 2. / Actin/actin-like conserved site / Actins and actin-related proteins signature. / Actin / Actin family / Actin / Helicase conserved C-terminal domain / ATPase, nucleotide binding domain / Nucleotidyltransferase; domain 5 / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / Alpha-Beta Complex / P-loop containing nucleoside triphosphate hydrolase / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
ADENOSINE-5'-TRIPHOSPHATE / Actin / Actin-related protein 4 / Helicase SWR1
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
Saccharomyces bayanus (yeast)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å
AuthorsChen, Z. / Cao, T.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation31270762 China
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2016
Title: Crystal structure of a nuclear actin ternary complex.
Authors: Cao, T. / Sun, L. / Jiang, Y. / Huang, S. / Wang, J. / Chen, Z.
History
DepositionFeb 20, 2016Deposition site: RCSB / Processing site: PDBJ
Revision 1.0Jul 27, 2016Provider: repository / Type: Initial release
Revision 1.1Dec 25, 2019Group: Database references / Derived calculations / Structure summary
Category: audit_author / citation ...audit_author / citation / citation_author / pdbx_struct_oper_list
Item: _audit_author.name / _citation.journal_id_CSD ..._audit_author.name / _citation.journal_id_CSD / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.name / _pdbx_struct_oper_list.symmetry_operation
Revision 1.2Nov 8, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Actin-related protein 4
B: Actin
C: Actin-related protein 4
D: Actin
H: Helicase SWR1
E: Helicase SWR1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)213,01512
Polymers211,9046
Non-polymers1,1126
Water00
1
A: Actin-related protein 4
B: Actin
E: Helicase SWR1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)106,5086
Polymers105,9523
Non-polymers5563
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area7620 Å2
ΔGint-59 kcal/mol
Surface area33960 Å2
MethodPISA
2
C: Actin-related protein 4
D: Actin
H: Helicase SWR1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)106,5086
Polymers105,9523
Non-polymers5563
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area7300 Å2
ΔGint-54 kcal/mol
Surface area33640 Å2
MethodPISA
Unit cell
Length a, b, c (Å)110.260, 202.220, 87.010
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number18
Space group name H-MP21212

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Components

#1: Protein Actin-related protein 4 / Actin-like protein ARP4 / Actin-like protein 4


Mass: 54991.797 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: ARP4, ACT3, YJL081C, J1012 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P80428
#2: Protein Actin


Mass: 41735.547 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces bayanus (yeast) / Gene: ACT1 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P60011
#3: Protein Helicase SWR1 / Swi2/Snf2-related 1


Mass: 9224.541 Da / Num. of mol.: 2 / Fragment: UNP residues 340-410
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: SWR1, YDR334W, D9651.6 / Production host: Escherichia coli (E. coli) / References: UniProt: Q05471, DNA helicase
#4: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg
#5: Chemical ChemComp-ATP / ADENOSINE-5'-TRIPHOSPHATE


Mass: 507.181 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H16N5O13P3 / Comment: ATP, energy-carrying molecule*YM

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.29 Å3/Da / Density % sol: 46.26 %
Crystal growTemperature: 291 K / Method: evaporation
Details: polyethylene glycol (PEG) 3350, citric acid, BIS-TRIS propane

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL17U / Wavelength: 0.987 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Jun 1, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.987 Å / Relative weight: 1
ReflectionResolution: 2.8→48.403 Å / Num. obs: 48531 / % possible obs: 99.7 % / Redundancy: 9.4 % / Rmerge(I) obs: 0.12 / Net I/σ(I): 15.4
Reflection shellResolution: 2.8→2.87 Å

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Processing

Software
NameVersionClassification
PHENIX(1.10.1_2155: ???)refinement
HKL-2000V705data reduction
HKL-2000V705data scaling
PHASER5phasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3QB0, 1YAG

3qb0

1yag


Resolution: 2.8→48.403 Å / SU ML: 0.52 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 34.97
RfactorNum. reflection% reflection
Rfree0.2844 1999 4.12 %
Rwork0.2317 --
obs0.2338 48531 99.63 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Refinement stepCycle: LAST / Resolution: 2.8→48.403 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms12589 0 66 0 12655
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00712940
X-RAY DIFFRACTIONf_angle_d0.70817524
X-RAY DIFFRACTIONf_dihedral_angle_d10.6297782
X-RAY DIFFRACTIONf_chiral_restr0.0461957
X-RAY DIFFRACTIONf_plane_restr0.0042230
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.8-2.870.41581390.3593237X-RAY DIFFRACTION99
2.87-2.94760.38561400.35353256X-RAY DIFFRACTION99
2.9476-3.03430.3771400.33473261X-RAY DIFFRACTION99
3.0343-3.13220.41811410.32953276X-RAY DIFFRACTION100
3.1322-3.24420.36471420.30183324X-RAY DIFFRACTION100
3.2442-3.3740.33651420.27513289X-RAY DIFFRACTION100
3.374-3.52750.29871410.26053277X-RAY DIFFRACTION100
3.5275-3.71350.32791430.25593328X-RAY DIFFRACTION100
3.7135-3.9460.28521430.24183323X-RAY DIFFRACTION100
3.946-4.25050.2781420.2093334X-RAY DIFFRACTION100
4.2505-4.6780.2431440.18893347X-RAY DIFFRACTION100
4.678-5.35410.24111450.20123372X-RAY DIFFRACTION100
5.3541-6.74270.30051450.22523397X-RAY DIFFRACTION100
6.7427-48.40980.23751520.20013511X-RAY DIFFRACTION98
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.647-0.0314-0.14892.2479-0.63262.622-0.0307-1.00031.55280.7288-0.1468-0.0894-0.9143-0.1735-0.1570.7339-0.0355-0.05540.8891-0.00040.944226.474227.291398.821
26.23352.7208-2.81551.1789-0.98163.16250.03120.69961.1739-0.27940.3118-0.5057-0.1142-0.055-0.41910.6039-0.10370.13050.9406-0.06770.608517.087215.5523100.6498
31.8996-0.2251-0.22293.4424-3.72812.97550.2606-0.6361-0.57450.3803-0.27950.28940.16920.16310.13260.6861-0.03950.01580.66620.08510.593419.9613195.3795112.5874
44.0987-0.04481.68652.7837-0.95131.41950.02340.081-0.32650.3299-0.02480.429-0.0212-0.5821-0.0140.5292-0.02240.09140.4901-0.00730.397413.3112202.1938101.3071
53.2583-1.38330.68392.8728-0.98742.10170.05420.5454-0.2735-0.13610.4760.2378-0.09160.0398-0.44490.55580.10580.02670.7935-0.04360.521431.5147201.466390.5342
61.65450.2679-0.71912.23860.61643.11560.0569-0.3588-0.41980.34880.1155-0.22360.78190.275-0.240.77890.1675-0.1940.82070.10980.856938.0642186.7999115.686
72.83170.52782.72361.25990.56712.65390.1750.2929-0.0255-0.64370.8013-0.3554-0.5880.2455-0.22020.83360.17410.60651.24450.14811.505160.3917189.41797.4194
83.155-1.39670.19331.3473-1.89145.29450.1513-0.148-0.76220.0557-0.0113-0.36020.66470.8921-0.28310.73510.2373-0.04490.8443-0.050.940447.0183186.8187105.7369
92.1875-0.5957-0.00952.3965-0.13752.74850.38230.8566-0.0066-0.6091-0.2706-0.77180.02920.5566-0.05540.66320.14390.09921.0754-0.14470.74942.0287200.096786.6016
104.0413-2.5192-2.51736.1756-0.45165.68670.1906-0.63420.3534-0.33730.0269-1.39250.63431.1958-0.04110.55560.01310.01890.6981-0.04550.783932.8273209.232102.2672
119.51124.63981.90585.53210.69293.1896-0.47781.09670.6242-0.40340.43521.2872-0.3723-0.45760.11280.57370.0226-0.01910.6010.07880.5159.4942211.264790.893
121.32720.352-0.25840.074-0.07140.04640.26360.6656-0.8687-0.35970.5711.8068-0.014-0.752-0.57460.5876-0.1604-0.06060.77340.00720.841315.4154206.001584.7322
133.759-0.0173-0.34335.1631-1.36172.7336-0.6058-1.47310.53661.3030.37930.13861.1608-0.86130.19371.89520.2195-0.07231.2187-0.00440.627124.5292227.8437134.0825
144.76142.140.56231.13480.41120.21680.0301-1.68051.4301-0.0541-0.44230.51471.5702-0.8346-0.00531.60510.29060.33311.91750.31240.70739.1497223.6939136.7276
152.3012-0.5364-0.37530.15140.60543.3631-0.1409-0.30660.20710.91250.58480.2442-0.024-0.7067-0.20541.50130.12160.38631.26270.07610.980810.0091223.5701131.0261
165.19520.43560.89491.9738-0.78021.668-0.1168-0.7810.3721.23490.2235-0.0118-0.8663-0.4464-0.15091.2020.08220.05160.7007-0.18760.580723.5673227.2763122.4378
172.8981-0.2298-0.08273.2644-0.33283.3133-0.3335-0.8781-0.3120.54160.02310.2104-0.17060.12710.0270.89630.1523-0.03140.85520.03110.618328.9717210.3475123.9553
182.2063-0.43520.2011.77981.07571.84050.19950.9678-0.232.3126-0.55760.71470.8262-0.96070.26112.1099-0.03170.06971.54870.10730.774317.2287203.5958144.8417
190.66610.6833-0.38810.7447-0.53220.3981.03560.1915-0.42770.11920.3070.27130.5838-0.1968-0.64582.41550.3405-0.16461.19640.40981.334130.9172191.1486148.9318
200.5811-0.3532-1.09030.10610.47541.8613-0.8214-1.3933-0.47280.76470.06530.51671.80281.1710.14031.88730.48580.34531.68360.19480.99820.1379201.4547151.8308
218.2617-0.1427-1.22660.40970.6451.108-0.61270.9638-1.88470.55740.3162-0.37450.0261-0.88930.86481.33030.03320.11751.40990.07040.403124.548196.9452131.1968
222.8425-0.34250.0323.33650.85231.6267-0.14-0.7382-0.53540.64080.063-0.1645-0.27150.7982-0.05250.9647-0.0139-0.15921.0710.13550.627336.6492205.638127.4701
232.02970.04260.47875.34741.66392.6971-0.2437-0.94460.39810.8714-0.1469-0.866-0.65950.66060.2361.18910.1085-0.36131.27390.07090.832141.4605203.7538132.2246
242.2834-0.8572-0.05361.0697-0.90762.486-0.3297-0.27231.08590.52720.0251-0.3208-0.45240.38330.18780.95060.0330.01120.925-0.24840.832928.6573230.8171121.2806
255.449-1.1580.71481.1994-0.31461.6417-0.20910.27090.48490.9919-0.072-0.88580.04460.75160.02150.91720.18480.1320.65860.27031.3225.0719243.921798.8988
260.02190.20450.02771.587-0.37712.0555-0.2265-0.954-0.818-0.44810.91571.27450.174-1.0035-0.58360.61840.07930.22680.85890.25311.4009-3.5798246.185993.2966
271.0659-1.893-0.90663.41371.43634.8245-0.89380.13280.0837-0.0521-0.61080.50511.2438-0.63370.43111.2418-0.0471-0.08841.3379-0.00242.6447-28.2314243.763889.7788
282.0532-1.12990.48491.5549-0.93281.38250.3403-0.15760.43270.056-0.17480.72380.17110.0029-0.30070.64920.05580.20120.96030.52051.9202-16.0083246.313293.2678
292.32490.14490.98550.6882-1.25112.93040.2417-0.24150.46970.42060.37620.73850.0161-0.8266-0.71260.74710.08660.24490.6520.21011.446-8.3122239.6491107.0271
300.7358-0.49690.15110.9031-0.06570.97140.2477-0.42370.67120.23790.18760.5649-0.2635-0.313-0.21690.91230.19410.39260.85520.21331.9616-2.9198261.1008106.7977
312.16311.4986-0.88621.4158-0.25256.25530.14681.0195-0.1945-0.49440.66521.0287-0.9812-1.6308-0.22830.79380.198-0.00751.45080.66432.4641-18.3599267.556676.3512
320.924-0.7863-0.15761.69020.01362.5439-0.12710.32631.3256-0.4471-0.16310.5976-0.6635-0.45340.03430.80670.14230.1011.14770.65832.218-15.7542264.083485.9229
330.6506-0.11230.00011.36640.0690.7265-0.6334-0.0846-0.2666-0.5341.0618-0.4457-1.61771.0498-0.04431.5654-0.15210.30151.65380.36772.44290.5174286.7997.1693
341.8368-0.11320.65392.1940.64191.99920.14640.1831.24910.56320.22940.5635-0.488-0.7114-0.21320.98710.18520.48480.81310.08442.2571-6.4745273.3165101.3167
350.92590.82160.77340.86660.21120.4921-0.43610.2640.09190.22250.63080.3893-0.36450.59-0.37580.98820.09030.47340.68670.10912.03576.7177265.4835103.9332
363.4040.31210.16213.0115-0.31263.1968-0.0776-0.6026-0.15151.35880.76790.71280.6512-0.5257-0.75911.07970.09930.3910.70480.20611.3239-0.9077235.4931111.8261
373.24052.2636-0.42612.3527-0.16740.57170.6224-0.01650.43-0.5019-0.53420.43040.1948-0.3187-0.19221.03310.1921-0.22151.73530.170.92537.7314231.3165.9778
381.0261.26241.65371.44122.23313.38560.05850.3092-0.7983-1.2674-0.0650.4651-0.3597-0.4866-0.04241.0081-0.0821-0.24581.07980.04251.1011-3.9642224.096670.7121
396.49111.57590.57664.85980.79332.86840.4951.5502-0.7158-1.8673-0.33640.26770.5680.3483-0.16851.0110.2019-0.17430.95410.13280.750611.4764224.080769.9264
403.9234-1.60880.77454.6-2.22331.91450.1251.08680.1969-0.313-0.40740.8601-0.1252-0.55230.19850.7502-0.0114-0.04840.84810.17640.6669.4456232.343781.6865
412.6455-1.64680.31952.373-1.04690.75450.03531.10490.5944-0.3707-0.07450.47270.2472-0.0042-0.06830.7130.0113-0.07420.93430.47080.95315.321246.150675.5859
421.0173-0.09650.84060.8066-0.22361.32960.44270.43650.8315-0.75360.14680.47961.1835-0.9582-0.18061.26770.2353-0.36941.8992-0.0591.5383-16.2099236.706260.6097
433.5745-0.46891.24581.3191-1.4941.8365-0.07661.5248-0.2666-0.33460.2647-0.2995-0.7534-0.1867-0.14311.2127-0.0609-0.45391.41510.87931.837-18.0083254.258155.9423
440.148-0.3357-0.19110.87570.46840.2861-0.4022-0.7520.3327-1.0087-0.0940.1768-0.3888-0.62030.08771.22870.2462-0.46172.09730.28081.4346-19.2586248.383651.9916
451.76150.32690.29660.91711.63442.57980.2270.2979-0.0493-0.9227-0.69921.6526-0.2604-1.3088-0.08950.83460.1158-0.17531.15890.49011.9331-13.9407250.641271.5843
461.5348-0.68-1.2163.37280.11380.8688-0.24070.91750.9877-0.23640.19240.1244-0.09050.88120.18170.89930.06780.03051.20180.56981.3170.6332257.717775.2528
471.2223-1.2548-0.24971.92860.70791.97660.37420.75011.0868-0.8186-0.02070.40080.2717-0.5023-0.110.6337-0.0213-0.15691.2320.73961.20385.2193250.888866.8723
484.6762-0.69650.91044.9613-1.01814.0799-0.41340.61790.93821.04640.170.1301-0.78450.40360.17070.6972-0.03250.1060.98210.26280.742519.77234.066485.8472
490.84040.92790.47921.01920.52010.2572-0.52370.09270.0785-0.8567-0.03420.15761.18080.13030.08421.3510.13340.0592.2160.05871.427121.679216.707479.7385
500.902-0.86710.61110.8305-0.59790.47210.5029-0.43130.310.29320.17080.3003-0.26480.8019-0.1410.72310.04430.06720.92540.15860.591325.4344220.254387.7228
515.8381.5928-0.99233.6714.80488.28220.1146-0.2673-0.1690.5241-0.1293-0.6606-0.79650.9889-0.27760.67250.0237-0.0330.99660.20450.758131.2534221.475999.0602
524.0787-0.3457-0.25764.12810.58173.71210.70580.89170.18280.494-0.9245-0.0590.84770.04990.19310.6911-0.1572-0.04480.9129-0.07010.686236.5276223.5623111.3521
535.1325-1.91914.4923.2324-2.39374.1424-0.5683-0.07680.42032.168-0.4088-1.0651.57091.2981-0.09391.4390.1071-0.03391.3621-0.27410.788340.7738227.6017120.668
540.0328-0.0003-0.0719-0.00330.00770.1426-0.78580.3552-0.05610.3546-0.0102-0.0457-1.4424-0.0840.15181.43610.2524-0.60291.1779-0.08861.659745.7079228.5509127.9789
550.3807-0.247-0.10910.20140.01410.0372-0.2316-0.33541.066-0.0036-0.0573-0.2731-1.03560.3113-0.1261.2202-0.0155-0.13760.9909-0.25991.362612.4438243.7009118.393
560.63780.3371.12341.7467-0.61963.12490.30140.4699-0.15931.4107-0.44990.8674-0.43251.21550.01180.91180.13960.20050.65830.13731.048215.0101246.2832107.0756
572.7031.43771.53452.5477-2.44346.8505-0.29650.15511.0368-0.4342-0.75141.0515-1.0969-0.23310.21920.7650.2242-0.10560.94460.58391.324514.289247.921695.6927
583.8391-2.275-2.89164.52382.27272.3582-0.9763-0.5424-0.56780.7677-0.59190.7686-0.3818-0.39550.71620.6441-0.00780.02120.96140.39781.051617.2778249.803884.7848
590.77320.7713-1.99960.7915-1.85165.9059-0.29160.31640.89840.49850.6465-0.4045-2.2011-0.39160.09740.24450.6897-0.99760.9792.25980.517420.7145251.745172.0122
600.1698-0.2572-0.19260.36930.26370.20520.4199-0.3810.55660.49460.8354-0.7207-0.9330.5528-0.34971.0479-0.32830.09842.1270.57381.277522.1309255.456962.9868
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1(chain A and resid 0:9)
2X-RAY DIFFRACTION2(chain A and resid 10:20)
3X-RAY DIFFRACTION3(chain A and resid 21:76)
4X-RAY DIFFRACTION4(chain A and resid 77:140)
5X-RAY DIFFRACTION5(chain A and resid 141:189)
6X-RAY DIFFRACTION6(chain A and resid 190:255)
7X-RAY DIFFRACTION7(chain A and resid 256:260)
8X-RAY DIFFRACTION8(chain A and resid 261:306)
9X-RAY DIFFRACTION9(chain A and resid 307:440)
10X-RAY DIFFRACTION10(chain A and resid 441:457)
11X-RAY DIFFRACTION11(chain A and resid 458:482)
12X-RAY DIFFRACTION12(chain A and resid 483:489)
13X-RAY DIFFRACTION13(chain B and resid 5:28)
14X-RAY DIFFRACTION14(chain B and resid 29:58)
15X-RAY DIFFRACTION15(chain B and resid 59:95)
16X-RAY DIFFRACTION16(chain B and resid 96:148)
17X-RAY DIFFRACTION17(chain B and resid 149:183)
18X-RAY DIFFRACTION18(chain B and resid 184:220)
19X-RAY DIFFRACTION19(chain B and resid 221:236)
20X-RAY DIFFRACTION20(chain B and resid 237:260)
21X-RAY DIFFRACTION21(chain B and resid 261:275)
22X-RAY DIFFRACTION22(chain B and resid 276:312)
23X-RAY DIFFRACTION23(chain B and resid 313:334)
24X-RAY DIFFRACTION24(chain B and resid 335:375)
25X-RAY DIFFRACTION25(chain C and resid 1:29)
26X-RAY DIFFRACTION26(chain C and resid 30:44)
27X-RAY DIFFRACTION27(chain C and resid 45:54)
28X-RAY DIFFRACTION28(chain C and resid 55:99)
29X-RAY DIFFRACTION29(chain C and resid 100:139)
30X-RAY DIFFRACTION30(chain C and resid 140:199)
31X-RAY DIFFRACTION31(chain C and resid 200:216)
32X-RAY DIFFRACTION32(chain C and resid 217:250)
33X-RAY DIFFRACTION33(chain C and resid 251:265)
34X-RAY DIFFRACTION34(chain C and resid 266:401)
35X-RAY DIFFRACTION35(chain C and resid 402:454)
36X-RAY DIFFRACTION36(chain C and resid 455:482)
37X-RAY DIFFRACTION37(chain D and resid 5:52)
38X-RAY DIFFRACTION38(chain D and resid 53:79)
39X-RAY DIFFRACTION39(chain D and resid 80:103)
40X-RAY DIFFRACTION40(chain D and resid 104:127)
41X-RAY DIFFRACTION41(chain D and resid 128:180)
42X-RAY DIFFRACTION42(chain D and resid 181:214)
43X-RAY DIFFRACTION43(chain D and resid 215:232)
44X-RAY DIFFRACTION44(chain D and resid 233:256)
45X-RAY DIFFRACTION45(chain D and resid 257:279)
46X-RAY DIFFRACTION46(chain D and resid 280:304)
47X-RAY DIFFRACTION47(chain D and resid 305:357)
48X-RAY DIFFRACTION48(chain D and resid 358:375)
49X-RAY DIFFRACTION49(chain E and resid 350:353)
50X-RAY DIFFRACTION50(chain E and resid 354:360)
51X-RAY DIFFRACTION51(chain E and resid 361:370)
52X-RAY DIFFRACTION52(chain E and resid 371:378)
53X-RAY DIFFRACTION53(chain E and resid 379:384)
54X-RAY DIFFRACTION54(chain E and resid 385:390)
55X-RAY DIFFRACTION55(chain H and resid 350:354)
56X-RAY DIFFRACTION56(chain H and resid 355:364)
57X-RAY DIFFRACTION57(chain H and resid 365:370)
58X-RAY DIFFRACTION58(chain H and resid 371:379)
59X-RAY DIFFRACTION59(chain H and resid 380:387)
60X-RAY DIFFRACTION60(chain H and resid 388:393)

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